##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933839.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742570
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.69143784424364	28.0	16.0	31.0	16.0	34.0
2	26.127239182837982	30.0	16.0	31.0	16.0	34.0
3	27.419600845711514	31.0	25.0	33.0	16.0	34.0
4	31.729183780653674	35.0	28.0	35.0	25.0	37.0
5	33.2428646457573	35.0	32.0	35.0	28.0	37.0
6	31.34665284080962	35.0	28.0	37.0	17.0	37.0
7	33.23742812125456	35.0	32.0	35.0	28.0	37.0
8	32.422956758285416	35.0	32.0	37.0	23.0	37.0
9	34.516992337422735	35.0	32.0	39.0	29.0	39.0
10	33.55721750138034	35.0	32.0	37.0	27.0	39.0
11	34.368015136620116	35.0	32.0	39.0	27.0	39.0
12	34.183361837941206	35.0	32.0	39.0	27.0	39.0
13	34.751891404177385	37.0	34.0	39.0	30.0	39.0
14	34.82429939264985	37.0	33.0	39.0	27.0	40.0
15	35.30019661446059	37.0	33.0	39.0	27.0	40.0
16	34.8275516112959	36.0	32.0	39.0	27.0	40.0
17	34.51037343280768	36.0	32.0	38.0	27.0	40.0
18	34.832545079925126	37.0	33.0	39.0	27.0	40.0
19	34.3561684420324	36.0	32.0	39.0	27.0	40.0
20	34.64408877277563	37.0	32.0	39.0	27.0	40.0
21	34.127237836163594	36.0	32.0	39.0	25.0	40.0
22	35.47319983301237	37.0	34.0	39.0	30.0	40.0
23	35.97599418236665	38.0	34.0	39.0	30.0	40.0
24	36.22357622850371	38.0	35.0	40.0	30.0	40.0
25	34.19447863501084	37.0	32.0	39.0	25.0	40.0
26	34.986638296726234	37.0	33.0	39.0	27.0	40.0
27	35.41757006073502	37.0	34.0	39.0	30.0	40.0
28	35.69128836338662	38.0	34.0	39.0	30.0	40.0
29	36.20943884078269	38.0	35.0	40.0	30.0	40.0
30	33.74192332036037	37.0	32.0	39.0	24.0	40.0
31	34.96877196762595	37.0	33.0	39.0	27.0	40.0
32	35.273504181423974	37.0	34.0	39.0	29.0	40.0
33	35.80672125186851	38.0	35.0	39.0	30.0	40.0
34	35.991553658240974	38.0	35.0	40.0	30.0	40.0
35	36.25311822454449	38.0	35.0	40.0	30.0	41.0
36	36.067422599889575	38.0	35.0	40.0	30.0	41.0
37	36.41905140256137	38.0	35.0	40.0	30.0	41.0
38	35.84236772290828	38.0	35.0	40.0	30.0	41.0
39	35.884798739512775	38.0	35.0	40.0	30.0	41.0
40	35.32934672825458	38.0	34.0	40.0	28.0	41.0
41	35.01768183470919	37.0	33.0	40.0	27.0	40.0
42	35.852768089203714	38.0	35.0	40.0	30.0	41.0
43	36.05627886933218	38.0	35.0	40.0	31.0	40.0
44	36.52161277724659	38.0	35.0	40.0	31.0	41.0
45	36.75930350000673	39.0	35.0	40.0	32.0	41.0
46	36.54886273347967	39.0	35.0	40.0	31.0	41.0
47	36.221997926121446	38.0	35.0	40.0	30.0	41.0
48	36.382506699705075	38.0	35.0	40.0	31.0	41.0
49	36.59788302786269	39.0	35.0	40.0	31.0	41.0
50	36.35301587729103	39.0	35.0	40.0	31.0	41.0
51	35.770220989267	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	11.0
11	12.0
12	13.0
13	13.0
14	28.0
15	45.0
16	106.0
17	250.0
18	601.0
19	1097.0
20	1607.0
21	2231.0
22	2604.0
23	3150.0
24	3938.0
25	5268.0
26	7137.0
27	9428.0
28	13109.0
29	17751.0
30	25082.0
31	36163.0
32	50114.0
33	67117.0
34	85143.0
35	107828.0
36	124393.0
37	113840.0
38	58759.0
39	5727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.524583540945635	3.151218066983584	35.22711663546871	13.097081756602073
2	14.544352720955601	28.318677027081623	33.6047780007272	23.53219225123557
3	14.276229850384475	28.33631846155918	42.302543867918175	15.084907820138168
4	15.239640707273388	3.0782283151756737	57.23433481018625	24.447796167364693
5	32.41135515843624	9.481261025896547	36.40518738974103	21.702196425926175
6	15.028751498175255	28.739916775522843	39.27723985617518	16.95409187012672
7	53.54969901827437	1.4610070431070472	32.88444187080006	12.104852067818522
8	48.76321424242832	19.00669297170637	20.739189571353542	11.490903214511764
9	46.06596011150464	3.795736428888859	21.564162301197193	28.57414115840931
10	40.77352976823734	18.23491388017291	24.116918270331418	16.874638081258333
11	28.79607309748576	24.676865480695422	27.55807533296524	18.968986088853576
12	20.933110683167914	14.77382603660261	32.1227628371736	32.17030044305587
13	21.246885815478674	19.56650551463162	38.9140417738395	20.272566896050204
14	17.81313546197665	27.03394966131139	25.339429279394537	29.813485597317424
15	11.028185894932465	21.926552378900304	45.4886408015406	21.556620924626635
16	16.008861117470406	24.16378253901989	25.51799830319027	34.30935804031943
17	13.879364908358808	27.010921529283433	32.784787966117676	26.324925596240085
18	12.27641838479874	26.954226537565486	34.51337921004081	26.255975867594973
19	12.736711690480359	26.492855892373786	31.330379627509863	29.440052789635995
20	15.322865184427057	25.451607255881605	44.30855003568687	14.916977524004471
21	19.17516193759511	35.29943305008282	27.558614002720283	17.96679100960179
22	17.509595055011648	22.52057045127059	32.45566074578828	27.514173747929487
23	15.938699381876456	33.621880765449724	28.78583837214	21.65358148053382
24	21.04879001306274	25.684581924936367	24.53075130964084	28.735876752360046
25	13.396312805526753	36.78979759483955	22.18726854033963	27.62662105929407
26	15.282464952799064	20.40117430006599	36.65405281656948	27.662307930565465
27	24.08486741990654	24.312994061165952	27.211306678158287	24.39083184076922
28	15.266574195025385	23.819976567865655	37.819599499037125	23.09384973807183
29	24.493044426788046	19.911792827612214	30.716026771886824	24.879135973712916
30	22.19952327726679	31.73909530414641	28.5004780694076	17.560903349179203
31	27.886663883539597	19.534319996767984	27.63739445439487	24.941621665297546
32	28.152497407651804	33.755470864699625	20.719258790417065	17.372772937231506
33	19.324777462057448	23.769610945769422	21.48282316818616	35.42278842398696
34	27.59699422276688	21.023068532259582	32.48986627523331	18.89007096974023
35	25.428309788976122	25.200317815155476	23.294100219507925	26.077272176360477
36	19.19253403719515	21.57870638458327	34.103855528771696	25.12490404944988
37	28.54828501016739	21.57022233594139	32.13528690897828	17.74620574491294
38	19.956367749841768	30.488304132943693	25.900049826952344	23.655278290262196
39	31.193557509729725	22.51222107006747	27.57585143488156	18.71836998532125
40	21.651965471268703	29.93239694574249	28.69008982318165	19.725547759807156
41	24.141293076746972	25.474096718154517	22.392771051887365	27.991839153211146
42	20.453829268621142	22.083574612494445	31.491172549389283	25.971423569495133
43	22.553294638889263	20.67145184965727	25.141602811856124	31.633650699597343
44	24.736388488627334	17.780949944113015	31.37010652194406	26.112555045315595
45	19.564754837927737	18.006787238913503	30.272297561172685	32.156160361986075
46	29.884590004982698	21.97247397551746	26.622944638215927	21.51999138128392
47	14.898124082578073	19.18593533269591	44.38140512005602	21.53453546467
48	22.482594233540272	22.340654753087254	26.533794793756815	28.642956219615662
49	19.502403813781864	18.548150342728633	38.77062095156012	23.17882489192938
50	24.00406695665055	15.846721521203389	31.378321235708416	28.770890286437645
51	19.870315256474136	16.21961565912978	27.723312280323743	36.186756804072346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	292.5
2	469.0
3	2001.0
4	3533.0
5	2357.5
6	1182.0
7	1392.5
8	1603.0
9	1884.5
10	2166.0
11	2198.5
12	2231.0
13	2133.5
14	2036.0
15	1883.0
16	1730.0
17	1660.5
18	1591.0
19	1660.5
20	1730.0
21	1933.5
22	2137.0
23	2183.0
24	2229.0
25	2747.5
26	3532.5
27	3799.0
28	4747.5
29	5696.0
30	10672.5
31	15649.0
32	12824.0
33	9999.0
34	10929.5
35	11860.0
36	12799.5
37	13739.0
38	17707.0
39	21675.0
40	22608.5
41	23542.0
42	24380.5
43	25219.0
44	31850.0
45	38481.0
46	118657.5
47	198834.0
48	157064.5
49	115295.0
50	104635.5
51	93976.0
52	69120.5
53	44265.0
54	35455.5
55	26646.0
56	22557.0
57	18468.0
58	16676.5
59	14885.0
60	13457.5
61	12030.0
62	10158.5
63	8287.0
64	7290.5
65	6294.0
66	5022.5
67	3751.0
68	2862.0
69	1973.0
70	1478.5
71	984.0
72	805.0
73	626.0
74	478.0
75	244.5
76	159.0
77	101.5
78	44.0
79	37.5
80	31.0
81	16.5
82	2.0
83	5.0
84	8.0
85	6.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742570.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.071043488238033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.78345276200125	19.43252971456016
2	11.761315340804416	6.367821581395948
3	4.597544674550051	3.7338099547158476
4	2.440938816937733	2.6431504346179864
5	1.5839324088946731	2.143935156180866
6	1.1416966331782155	1.8544151524285446
7	0.9483942003963438	1.7971814450045511
8	0.7991724173034107	1.7307545010736751
9	0.6893075412871282	1.6794246984258854
>10	4.029109408383018	17.0246660164717
>50	0.11130874938326017	2.1199057754287742
>100	0.08662943390903936	4.96380818498786
>500	0.010576849488894341	2.0995902389888492
>1k	0.010073189989423182	6.155743886607479
>5k	0.004029275995769273	8.263651224406532
>10k+	0.0025182974973557955	17.989612034705356
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	38503	5.185100394575596	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	34824	4.689658887377621	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	29117	3.921111814374403	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	14932	2.010854195564055	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	14565	1.961431245539141	TruSeq Adapter, Index 15 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	8647	1.1644693429575663	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	8264	1.112891713912493	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	8123	1.0939036050473356	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	8070	1.0867662307930566	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	7723	1.0400366295433427	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	7490	1.008659116312267	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	7097	0.9557348128795937	TruSeq Adapter, Index 14 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	5194	0.6994626769193477	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	4226	0.5691045961996849	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	3794	0.5109282626553725	TruSeq Adapter, Index 15 (95% over 22bp)
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	3604	0.48534144929097595	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCTGT	3172	0.42716511574666366	No Hit
TGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCTT	2968	0.39969295823962725	TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGT	2631	0.3543100313775132	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATG	2351	0.3166031485247182	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2321	0.31256312536191877	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	2286	0.30784976500531935	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	2071	0.27889626567192316	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	2012	0.2709508867850842	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTAT	1964	0.2644868497246051	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGAGAC	1905	0.25654147083776613	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTT	1674	0.22543329248421026	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	1635	0.22018126237257096	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCGACTCGTATGCCGTCTTCTGC	1608	0.21654524152605142	TruSeq Adapter, Index 14 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	1363	0.1835517190298558	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	1296	0.17452900063293697	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	1175	0.1582342405429791	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	1092	0.1470568431259006	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	989	0.1331860969336224	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	941	0.12672205987314328	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGT	922	0.12416337853670362	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	906	0.1220086995165439	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	904	0.12173936463902393	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGCGACTCGTATGCCGTCTTCTGC	879	0.11837267867002438	TruSeq Adapter, Index 14 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTGTGAGACTCG	822	0.11069663466070538	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	810	0.1090806253955856	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCTGTGAGAC	788	0.106117941742866	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	766	0.10315525809014639	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	747	0.10059657675370673	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.0482513433077016	0.0
2	0.0	0.0	0.0	5.275192911106024	0.0
3	0.0	0.0	0.0	6.4177114615457125	0.0
4	0.0	0.0	0.0	8.964946065690777	0.0
5	0.0	0.0	0.0	15.231156658631509	0.0
6	0.0	0.0	0.0	16.43400622163567	0.0
7	0.0	0.0	0.0	18.599458636896184	0.0
8	0.0	0.0	0.0	21.317047551072626	0.0
9	0.0	0.0	0.0	22.077783912627766	0.0
10	0.0	0.0	0.0	28.366079965525135	0.0
11	0.0	0.0	0.0	30.583514012147003	0.0
12	0.0	0.0	0.0	37.91656005494431	0.0
13	0.0	0.0	0.0	39.571488209865734	0.0
14	0.0	0.0	0.0	40.06948839840015	0.0
15	0.0	0.0	0.0	42.18390185437063	0.0
16	0.0	0.0	0.0	43.21060640747674	0.0
17	0.0	0.0	0.0	44.24970036494876	0.0
18	1.3466743875998222E-4	0.0	0.0	45.19048709212599	0.0
19	1.3466743875998222E-4	0.0	0.0	48.20851906217596	0.0
20	1.3466743875998222E-4	0.0	0.0	49.363965686736606	0.0
21	2.6933487751996444E-4	0.0	0.0	50.322259180952635	0.0
22	2.6933487751996444E-4	0.0	0.0	51.3915186447069	0.0
23	2.6933487751996444E-4	0.0	0.0	52.390347037989685	0.0
24	2.6933487751996444E-4	0.0	0.0	53.13020994653703	0.0
25	2.6933487751996444E-4	0.0	0.0	54.31218605653339	0.0
26	2.6933487751996444E-4	0.0	0.0	54.95185639064331	0.0
27	2.6933487751996444E-4	0.0	0.0	55.652800409389016	0.0
28	2.6933487751996444E-4	0.0	0.0	56.20614891525378	0.0
29	2.6933487751996444E-4	0.0	0.0	56.82077110575434	0.0
30	2.6933487751996444E-4	0.0	0.0	57.50811371318529	0.0
31	2.6933487751996444E-4	0.0	0.0	58.09809176239277	0.0
32	2.6933487751996444E-4	0.0	0.0	58.86825484466111	0.0
33	2.6933487751996444E-4	0.0	0.0	59.39372719070256	0.0
34	2.6933487751996444E-4	0.0	0.0	60.190285090967855	0.0
35	2.6933487751996444E-4	0.0	0.0	60.77487644262494	0.0
36	2.6933487751996444E-4	0.0	0.0	61.30573548621679	0.0
37	2.6933487751996444E-4	0.0	0.0	61.90433225150491	0.0
38	2.6933487751996444E-4	0.0	0.0	62.43963532057584	0.0
39	2.6933487751996444E-4	0.0	0.0	63.16266479927818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	35	1.2111559E-7	45.000004	1
ACCGGTC	35	1.2111559E-7	45.000004	17
ATAGGTA	20	7.0316705E-4	45.000004	33
TCTTACG	35	1.2111559E-7	45.000004	1
TTCCGCG	20	7.0316705E-4	45.000004	1
ATCGACG	20	7.0316705E-4	45.000004	16
TAGCGGG	65	0.0	44.999996	3
CTCAACT	3495	0.0	44.4206	2
GCTCAAC	3685	0.0	44.267296	1
TTGACCC	3170	0.0	44.148266	8
CTTGACC	3630	0.0	44.070248	7
TACGGCT	4140	0.0	44.02174	7
ACCCTGC	2735	0.0	44.0128	11
CTGCATT	2665	0.0	43.986866	14
TGCATTA	2650	0.0	43.896225	15
ACTTGAC	3345	0.0	43.856506	6
TCAACTT	3420	0.0	43.81579	3
CACCGGT	180	0.0	43.75	16
GCATTAG	2655	0.0	43.728813	16
CCTGCAT	2760	0.0	43.695652	13
>>END_MODULE