SRR2933838.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933838.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483427
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	27226	5.631874098881527	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	22115	4.574630709496987	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	16481	3.409201389248015	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	8832	1.8269562932976435	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	8471	1.7522811096608175	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA	5497	1.1370899846305647	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	4975	1.0291109102305	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	4086	0.8452155134074016	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	3780	0.7819174353108123	No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	2839	0.5872655023405808	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2623	0.542584506037106	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2240	0.463358480184185	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGT	1926	0.3984055503726519	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATG	1601	0.3311771994530715	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.2842207820415492	No Hit
TGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCTT	1329	0.2749122411449919	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTAT	1135	0.2347820870576116	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	1118	0.2312655271633566	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTGTCTT	1114	0.23043810130588485	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	862	0.17831027228516405	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	602	0.12452759154949972	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	592	0.12245902690582032	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCG	536	0.11087506490121568	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	489	0.10115281107592251	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8012014E-9	45.000004	1
CCTCGAA	20	7.029225E-4	45.000004	42
GGAACTA	40	6.8012014E-9	45.000004	8
TTGCGGA	20	7.029225E-4	45.000004	3
CGTACTT	20	7.029225E-4	45.000004	40
GTATATG	20	7.029225E-4	45.000004	16
CTTTACG	20	7.029225E-4	45.000004	1
TTGCACG	20	7.029225E-4	45.000004	1
TTTCTCG	35	1.2099736E-7	45.000004	1
CCTTGCG	20	7.029225E-4	45.000004	1
TCGTTTA	25	3.887441E-5	45.0	9
CCGATAG	25	3.887441E-5	45.0	20
ATTGTCG	25	3.887441E-5	45.0	1
TAAGTTG	25	3.887441E-5	45.0	15
GTCGTTT	25	3.887441E-5	45.0	8
CCGGTCG	25	3.887441E-5	45.0	14
CACCGTG	25	3.887441E-5	45.0	16
CGCAATA	45	3.8380676E-10	45.0	28
CCACCGT	45	3.8380676E-10	45.0	15
ACGGCTG	3195	0.0	43.80282	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content