##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933838.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483427
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.31194368539614	28.0	16.0	31.0	16.0	34.0
2	26.308844975559907	30.0	16.0	33.0	16.0	34.0
3	27.3964424825258	31.0	25.0	33.0	16.0	34.0
4	31.43944380433861	35.0	28.0	35.0	19.0	37.0
5	33.33532467156365	35.0	32.0	35.0	28.0	37.0
6	31.839725542843077	35.0	30.0	37.0	17.0	37.0
7	33.13530481334307	35.0	32.0	35.0	28.0	37.0
8	32.27697873722403	35.0	32.0	37.0	17.0	37.0
9	34.230363632978715	35.0	32.0	39.0	28.0	39.0
10	33.48558727584516	35.0	32.0	37.0	27.0	39.0
11	34.02736711023588	35.0	32.0	39.0	27.0	39.0
12	34.238484817769795	35.0	32.0	39.0	27.0	39.0
13	34.50163106322154	35.0	33.0	38.0	27.0	39.0
14	34.40806574725863	37.0	32.0	39.0	26.0	40.0
15	34.9314022592863	37.0	32.0	39.0	27.0	40.0
16	34.68079151557526	36.0	32.0	39.0	27.0	40.0
17	34.112076900959195	36.0	32.0	38.0	27.0	40.0
18	34.36329580267548	36.0	32.0	39.0	27.0	40.0
19	34.13013753886316	36.0	32.0	39.0	27.0	40.0
20	34.17242106874461	36.0	32.0	39.0	25.0	40.0
21	33.97367337777989	36.0	32.0	39.0	25.0	40.0
22	35.10038123646383	37.0	33.0	39.0	27.0	40.0
23	35.529614605721235	37.0	34.0	39.0	29.0	40.0
24	35.79851352944705	38.0	34.0	39.0	30.0	40.0
25	33.7967635237585	36.0	32.0	39.0	24.0	40.0
26	34.693213246260555	37.0	33.0	39.0	27.0	40.0
27	34.97329276188545	37.0	33.0	39.0	27.0	40.0
28	35.09124024930341	37.0	33.0	39.0	27.0	40.0
29	35.536726330966204	38.0	34.0	40.0	28.0	40.0
30	33.820036944564535	37.0	32.0	39.0	24.0	40.0
31	34.33585836124172	36.0	32.0	39.0	25.0	40.0
32	34.7548233756079	37.0	33.0	39.0	27.0	40.0
33	35.29797053122809	38.0	34.0	39.0	28.0	40.0
34	35.58959677469401	38.0	35.0	40.0	30.0	40.0
35	35.717436138238035	38.0	35.0	40.0	30.0	41.0
36	35.54756974682837	38.0	34.0	40.0	28.0	41.0
37	36.01588450789881	38.0	35.0	40.0	30.0	41.0
38	35.38618860758708	38.0	34.0	40.0	29.0	41.0
39	35.2744674997466	38.0	34.0	40.0	28.0	41.0
40	34.82189658417921	37.0	33.0	40.0	26.0	41.0
41	34.412215287933854	37.0	33.0	39.0	26.0	40.0
42	35.31964908869384	37.0	34.0	40.0	29.0	41.0
43	35.321959675400834	38.0	34.0	40.0	28.0	40.0
44	35.958775575216116	38.0	35.0	40.0	30.0	41.0
45	36.112573356473675	38.0	35.0	40.0	31.0	41.0
46	35.81377539938812	38.0	35.0	40.0	30.0	41.0
47	35.34250465944186	38.0	34.0	40.0	28.0	41.0
48	35.570189501207004	38.0	34.0	40.0	29.0	41.0
49	35.885142948159704	38.0	35.0	40.0	30.0	41.0
50	35.561427061376385	38.0	35.0	40.0	29.0	41.0
51	35.052483622139434	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	7.0
11	5.0
12	13.0
13	11.0
14	15.0
15	34.0
16	97.0
17	256.0
18	713.0
19	1535.0
20	2184.0
21	2587.0
22	2752.0
23	2866.0
24	3340.0
25	4313.0
26	6028.0
27	7841.0
28	10220.0
29	13760.0
30	18588.0
31	25668.0
32	34209.0
33	44316.0
34	53123.0
35	63292.0
36	72179.0
37	69342.0
38	39846.0
39	4278.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.78261867872502	3.385412895845702	39.11614369904866	13.715824726380612
2	15.728744981145033	28.581150825253864	38.112476133935424	17.577628059665678
3	15.638141849751877	28.665134549787247	39.67506986577084	16.021653734690037
4	16.107499167402732	3.372380938590521	60.4188429690522	20.101276924954544
5	34.36051358322973	4.006396001878257	38.63582298878631	22.9972674261057
6	16.559894254975415	24.737344004368808	42.104599039772296	16.598162700883485
7	60.77649779594437	1.8060637903964818	32.373243530046935	5.04419488361221
8	57.26593673915607	20.67675160882615	12.800484871552479	9.256826780465303
9	53.312702848620376	4.0696940799748464	13.81842553270711	28.799177538697673
10	39.266735205108525	22.268098389208713	21.336210017231146	17.128956388451616
11	33.35415688407971	20.796935214623925	26.872516429574684	18.976391471721687
12	24.01851779069022	16.32945615366953	30.17808273017436	29.47394332546589
13	24.674252782736588	19.92896549013605	38.95624365209225	16.440538075035114
14	19.885939345547516	28.879230990408068	25.24248749035532	25.9923421736891
15	12.388013081602807	23.686720021844042	41.55642940919726	22.3688374873559
16	14.767482991227219	24.064853638708637	25.99130789136726	35.176355478696884
17	15.35454163710343	27.928518680173013	34.55640665498617	22.160533027737383
18	14.501879290978783	23.66086296379805	32.66367000601952	29.173587739203644
19	15.336751981167787	27.52721713929921	28.76918335136432	28.36684752816868
20	19.943652299106173	24.77437131149067	39.55633425522364	15.725642134179516
21	21.91168470110275	32.95719932895763	26.524790712972173	18.606325256967445
22	16.66849389876858	21.926164653608506	34.09863329933992	27.306708148282986
23	18.63363031026401	31.886717125853544	27.811644777805128	21.66800778607732
24	22.470610867824924	22.671468494726195	25.304958142594437	29.552962494854444
25	15.636073285108196	35.68832522800754	22.125987998187938	26.549613488696327
26	19.820779559271617	22.239759053590305	30.268892718031886	27.670568669106192
27	21.903203586063665	27.399379844319828	23.800284220782043	26.897132348834468
28	15.79845560963703	25.521950573716406	35.8827703045134	22.796823512133166
29	27.72331706752002	18.370095174659255	26.570092278668756	27.336495479151974
30	17.452686755187443	27.293055621634704	31.786805453563826	23.467452169614024
31	23.663965810763568	19.57337922788756	27.183628551984064	29.579026409364808
32	28.39684171550203	27.318912679680697	25.658268983734878	18.625976621082398
33	18.30038454616726	18.130348532456814	30.388455754436556	33.18081116693937
34	28.66389341100104	19.711559346085345	27.174940580480612	24.449606662433006
35	19.07485514876083	18.646041698126087	30.58786538608725	31.691237767025836
36	23.28541848097024	20.437211823088077	33.861368934709894	22.41600076123179
37	20.996344846274617	19.1846959313402	32.52921330418036	27.289745918204815
38	19.83939664106473	20.425834717547843	36.033154954108895	23.701613687278535
39	27.728074766200482	20.374120601455857	28.501924799400946	23.395879832942722
40	18.75815790181351	18.474350832700697	39.3327224172419	23.43476884824389
41	17.689951119817472	21.31945464361734	31.090940307430078	29.899653929135113
42	19.021899893882637	17.768763432741654	39.68479211959613	23.524544553779577
43	24.597922747384818	18.06539560264528	32.622091856681564	24.714589793288336
44	21.320902638867917	17.33974312564255	32.09419415961459	29.24516007587495
45	19.09367908701831	15.343164531563195	32.3780012287274	33.185155152691095
46	30.209111199829554	21.549685888458857	27.934724374104054	20.30647853760754
47	14.674190725797276	18.544061461192694	45.47408398786166	21.307663825148367
48	20.784730683226215	21.665111795576166	28.178608145593852	29.371549375603763
49	19.251717425795416	15.065563156381417	42.00758335798373	23.67513605983944
50	23.63748818332447	15.911606095646292	33.23376642181757	27.217139299211667
51	19.767617447929055	15.87975020013363	29.23233497508414	35.12029737685317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	81.0
1	210.5
2	340.0
3	1394.0
4	2448.0
5	1732.5
6	1017.0
7	1091.5
8	1166.0
9	1308.0
10	1450.0
11	1482.0
12	1514.0
13	1443.5
14	1373.0
15	1305.0
16	1237.0
17	1251.5
18	1266.0
19	1285.5
20	1305.0
21	1403.0
22	1501.0
23	1722.5
24	1944.0
25	2233.5
26	3007.0
27	3491.0
28	3946.5
29	4402.0
30	5093.5
31	5785.0
32	6497.5
33	7210.0
34	7963.0
35	8716.0
36	9460.0
37	10204.0
38	11013.0
39	11822.0
40	13024.0
41	14226.0
42	16304.5
43	18383.0
44	21114.0
45	23845.0
46	72436.5
47	121028.0
48	91832.0
49	62636.0
50	56986.5
51	51337.0
52	40226.5
53	29116.0
54	25342.0
55	21568.0
56	19355.0
57	17142.0
58	16481.5
59	15821.0
60	14270.5
61	12720.0
62	11041.5
63	9363.0
64	7475.0
65	5587.0
66	4637.5
67	3688.0
68	3123.5
69	2559.0
70	2101.0
71	1643.0
72	1315.0
73	987.0
74	745.0
75	398.0
76	293.0
77	200.5
78	108.0
79	67.0
80	26.0
81	18.5
82	11.0
83	21.5
84	32.0
85	17.0
86	2.0
87	2.5
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483427.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.464048525445342
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18595673489365	21.858186291434954
2	10.419471747103076	6.139996423323036
3	3.766907264211252	3.3296501527071865
4	1.899090663382076	2.238195978404387
5	1.2045341370995624	1.7745226133028469
6	0.9059716572126687	1.6016155722475323
7	0.7520858855060252	1.551164651809644
8	0.6375744020057242	1.5028418495422764
9	0.5772291138688465	1.5306755959198357
>10	5.4790948697499395	27.637309915617514
>50	0.09064481973579538	1.8334373209571688
>100	0.06515096418510294	3.6814803790791264
>500	0.0028326506167436058	0.5408295818733335
>1k	0.009206114504416718	6.288813116382048
>5k	0.0021244879625577045	4.757297247959878
>10k+	0.0021244879625577045	13.733983309439257
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	27226	5.631874098881527	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	22115	4.574630709496987	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	16481	3.409201389248015	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTGTCTTT	8832	1.8269562932976435	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	8471	1.7522811096608175	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTA	5497	1.1370899846305647	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	4975	1.0291109102305	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	4086	0.8452155134074016	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	3780	0.7819174353108123	No Hit
CTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCT	2839	0.5872655023405808	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2623	0.542584506037106	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	2240	0.463358480184185	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGT	1926	0.3984055503726519	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATG	1601	0.3311771994530715	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1374	0.2842207820415492	No Hit
TGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGCTT	1329	0.2749122411449919	TruSeq Adapter, Index 16 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTAT	1135	0.2347820870576116	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	1118	0.2312655271633566	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTGTCTT	1114	0.23043810130588485	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	862	0.17831027228516405	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	602	0.12452759154949972	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTG	592	0.12245902690582032	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCTGTCTTTCG	536	0.11087506490121568	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	489	0.10115281107592251	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6594584084049918	0.0
2	0.0	0.0	0.0	3.646052040949306	0.0
3	0.0	0.0	0.0	4.4455522757313926	0.0
4	0.0	0.0	0.0	6.533561427061376	0.0
5	0.0	0.0	0.0	11.692561648397794	0.0
6	0.0	0.0	0.0	12.686093246757007	0.0
7	0.0	0.0	0.0	14.230690466192414	0.0
8	0.0	0.0	0.0	16.09239864550387	0.0
9	0.0	0.0	0.0	16.5406566037892	0.0
10	0.0	0.0	0.0	22.314020524298396	0.0
11	0.0	0.0	0.0	24.05223539438219	0.0
12	0.0	0.0	0.0	31.6089088942074	0.0
13	0.0	0.0	0.0	32.44543643611134	0.0
14	0.0	0.0	0.0	32.856253374346075	0.0
15	0.0	0.0	0.0	34.40622886185505	0.0
16	0.0	0.0	0.0	35.35963030612688	0.0
17	0.0	0.0	0.0	36.25366394512512	0.0
18	0.0	0.0	0.0	36.95594164165427	0.0
19	0.0	0.0	0.0	39.655625358120254	0.0
20	0.0	0.0	0.0	40.51180426413916	0.0
21	0.0	0.0	0.0	41.31068392952814	0.0
22	0.0	0.0	0.0	42.186514199662	0.0
23	0.0	0.0	0.0	42.93223175370842	0.0
24	0.0	0.0	0.0	43.515153270297276	0.0
25	0.0	0.0	0.0	44.07552743227002	0.0
26	0.0	0.0	0.0	44.5757063631117	0.0
27	0.0	0.0	0.0	45.068438461236134	0.0
28	0.0	0.0	0.0	45.535313501314576	0.0
29	0.0	0.0	0.0	46.01211765168267	0.0
30	0.0	0.0	0.0	46.59524602473589	0.0
31	0.0	0.0	0.0	47.09273582154079	0.0
32	0.0	0.0	0.0	47.58774334077327	0.0
33	0.0	0.0	0.0	48.044068701168946	0.0
34	0.0	0.0	0.0	48.49398151116922	0.0
35	0.0	0.0	0.0	48.965614249928116	0.0
36	0.0	0.0	0.0	49.43455785465024	0.0
37	0.0	0.0	0.0	49.90701801926661	0.0
38	0.0	0.0	0.0	50.379685040347354	0.0
39	0.0	0.0	0.0	50.86331545403959	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	40	6.8012014E-9	45.000004	1
CCTCGAA	20	7.029225E-4	45.000004	42
GGAACTA	40	6.8012014E-9	45.000004	8
TTGCGGA	20	7.029225E-4	45.000004	3
CGTACTT	20	7.029225E-4	45.000004	40
GTATATG	20	7.029225E-4	45.000004	16
CTTTACG	20	7.029225E-4	45.000004	1
TTGCACG	20	7.029225E-4	45.000004	1
TTTCTCG	35	1.2099736E-7	45.000004	1
CCTTGCG	20	7.029225E-4	45.000004	1
TCGTTTA	25	3.887441E-5	45.0	9
CCGATAG	25	3.887441E-5	45.0	20
ATTGTCG	25	3.887441E-5	45.0	1
TAAGTTG	25	3.887441E-5	45.0	15
GTCGTTT	25	3.887441E-5	45.0	8
CCGGTCG	25	3.887441E-5	45.0	14
CACCGTG	25	3.887441E-5	45.0	16
CGCAATA	45	3.8380676E-10	45.0	28
CCACCGT	45	3.8380676E-10	45.0	15
ACGGCTG	3195	0.0	43.80282	8
>>END_MODULE