FastQCFastQC Report
Sat 14 Jan 2017
SRR2933837.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933837.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498361
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC351817.059340518218721No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG296735.954117597484554No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC236794.751375007273843No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTAGACT110852.224291226640929No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC96681.9399591862124044TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTA66561.3355780247651803No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT64521.2946438425157667No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTC54961.1028150276606716No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT53381.0711111021929887No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT41500.8327296879169919Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC32370.6495291565752537TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC27400.5498022517813392TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGT25300.5076641229951783No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATG20650.41435826639725015No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTAT15720.31543399262783406No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15440.3098155754563459No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTT14530.29155571964900945No Hit
TGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCTT14130.2835294094040264TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGGTAGAC13250.26587152686506366No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG12300.2468090400332289No Hit
GCTGTCTCTTATACACATCTGACGCAGGTCGACTCGTATGCCGTCTTCTGC9410.18881894851322636TruSeq Adapter, Index 21 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG7360.1476841085076882No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC6670.13383872333509245No Hit
TTCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT6630.13303609231059413No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGGTAGACTCG6560.1316314880177221No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC6470.1298255682126009No Hit
TGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG6290.12621372860235852No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC5900.11838807611350005No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT5330.1069505840143992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG1350.045.00000417
CCCTCGA700.045.00000441
TCTCTCG453.8380676E-1045.0000041
GCGCGCG351.2100827E-745.0000041
CGCATGG351.2100827E-745.0000042
CAACATA351.2100827E-745.00000410
CGTTCGG351.2100827E-745.0000042
AGGCACG453.8380676E-1045.00000410
CGAGGCA453.8380676E-1045.00000434
CTTCTCG351.2100827E-745.0000041
CTTTCCG351.2100827E-745.0000041
CCGGTGA1150.045.00000418
TTGTTCG453.8380676E-1045.0000041
CGACTGA351.2100827E-745.00000441
TCACGGG207.029437E-445.03
CTTGTCG253.887615E-545.01
AACGTAG207.029437E-445.045
CGGGTAT253.887615E-545.06
CGGGTAC207.029437E-445.06
TCGTTCG207.029437E-445.01
Produced by FastQC (version 0.11.5)