##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933837.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	498361
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.48253976535082	28.0	16.0	31.0	16.0	33.0
2	25.334169407317187	28.0	16.0	31.0	16.0	34.0
3	26.49846797803199	30.0	16.0	33.0	16.0	34.0
4	31.098121642744918	33.0	28.0	35.0	19.0	37.0
5	33.217214027582415	35.0	32.0	35.0	28.0	37.0
6	31.512094646250407	35.0	28.0	36.0	17.0	37.0
7	33.138160891402016	35.0	32.0	35.0	28.0	37.0
8	32.00950515790762	35.0	31.0	36.0	17.0	37.0
9	34.206312291692164	35.0	32.0	38.0	28.0	39.0
10	33.435134370466386	35.0	32.0	37.0	27.0	39.0
11	34.15943262012878	35.0	32.0	39.0	27.0	39.0
12	34.35409873565548	35.0	33.0	39.0	27.0	39.0
13	34.620217071560575	35.0	33.0	38.0	29.0	39.0
14	34.57723016046601	36.0	33.0	39.0	27.0	40.0
15	35.110104923940675	37.0	33.0	39.0	27.0	40.0
16	34.897112735547125	36.0	32.0	39.0	27.0	40.0
17	34.134583163610316	36.0	32.0	38.0	27.0	40.0
18	34.63098637333178	36.0	32.0	39.0	27.0	40.0
19	34.28484773086176	36.0	32.0	39.0	27.0	40.0
20	33.89389418513888	36.0	32.0	39.0	25.0	40.0
21	33.90222549517318	36.0	32.0	39.0	25.0	40.0
22	35.151079237741314	37.0	33.0	39.0	28.0	40.0
23	35.44441679826471	37.0	34.0	39.0	29.0	40.0
24	35.88283192304374	38.0	34.0	39.0	30.0	40.0
25	33.723469533129595	36.0	32.0	39.0	24.0	40.0
26	34.824488673872956	37.0	33.0	39.0	27.0	40.0
27	34.78092788159587	37.0	33.0	39.0	27.0	40.0
28	35.0230254775153	37.0	33.0	39.0	27.0	40.0
29	35.26355192320426	37.0	34.0	40.0	27.0	40.0
30	33.29100190424211	36.0	31.0	39.0	23.0	40.0
31	34.51453464456488	37.0	33.0	39.0	25.0	40.0
32	34.519974476333424	37.0	33.0	39.0	26.0	40.0
33	34.901160002488155	37.0	34.0	39.0	27.0	40.0
34	34.93339968416469	37.0	34.0	40.0	26.0	40.0
35	35.26128449056006	38.0	34.0	40.0	27.0	41.0
36	35.3435662100365	38.0	34.0	40.0	27.0	41.0
37	35.249718577497035	38.0	34.0	40.0	27.0	41.0
38	34.9533851966747	37.0	34.0	40.0	27.0	41.0
39	34.49947126681261	37.0	33.0	40.0	25.0	40.0
40	34.23021263702417	37.0	33.0	39.0	24.0	40.0
41	34.10626032133333	37.0	33.0	39.0	24.0	40.0
42	34.68618330888653	37.0	33.0	40.0	26.0	40.0
43	35.04820802590893	38.0	34.0	40.0	27.0	40.0
44	35.602031459123005	38.0	35.0	40.0	30.0	41.0
45	35.9608617046679	38.0	35.0	40.0	30.0	41.0
46	35.48958084601323	38.0	35.0	40.0	28.0	41.0
47	34.96861311378699	38.0	34.0	40.0	26.0	41.0
48	35.141869046735195	38.0	34.0	40.0	26.0	41.0
49	35.464731389494766	38.0	35.0	40.0	28.0	41.0
50	35.16241038122967	38.0	34.0	40.0	26.0	41.0
51	34.806824771601306	37.0	34.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	7.0
11	5.0
12	7.0
13	8.0
14	21.0
15	41.0
16	159.0
17	601.0
18	1383.0
19	2370.0
20	3165.0
21	3375.0
22	3364.0
23	3384.0
24	3608.0
25	4522.0
26	6003.0
27	8180.0
28	10890.0
29	14784.0
30	19860.0
31	26756.0
32	36228.0
33	46946.0
34	57164.0
35	68034.0
36	72461.0
37	64799.0
38	36281.0
39	3952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.41425793751919	3.1615636054988254	34.696133927012745	11.728044529969239
2	14.292049337729077	36.059202064367	33.0260594227879	16.622689175116033
3	13.275517145201974	36.39329722831442	36.20066578243482	14.130519844048791
4	14.382345327985135	2.7510178364679416	65.2789845112278	17.587652324319116
5	38.63705225729942	3.342356243767068	36.94711263521825	21.07347886371526
6	14.22161846532935	30.073179883658636	39.62208118211497	16.083120468897043
7	54.372633492588704	1.1132492309791497	38.9683382126611	5.545779063771041
8	49.731419593427255	25.266824651206655	14.790081888430272	10.211673866935815
9	47.14273388166409	3.1932675309665077	14.445351863408252	35.21864672396114
10	40.288866905716944	21.459945702011193	21.507300932456594	16.743886459815275
11	32.21098761740987	18.72457917052097	29.76657483230028	19.297858379768883
12	20.147844634712587	16.003860655227836	31.719576772660783	32.12871793739879
13	22.48309960049041	20.721123843960505	43.02523672598779	13.7705398295613
14	18.98764148880029	31.988458165867716	24.57194684174725	24.45195350358475
15	9.068927945806353	23.219112249955355	45.095824111437295	22.616135692801002
16	11.096173255932948	26.006248482525717	24.18146684832882	38.71611141321251
17	12.207014593838602	30.57121243435983	35.62517933786953	21.59659363393203
18	11.495883506133104	24.217585244431245	34.129075108204695	30.157456141230952
19	12.010971966104892	27.809961052329534	29.3405784160478	30.838488565517768
20	16.821139695923236	26.47418236980823	42.63897054544798	14.065707388820552
21	18.238184769674994	37.606072706331354	27.334201512558167	16.821541011435485
22	13.072250838247776	22.202780715184375	36.43282680627096	28.29214164029689
23	16.300232161023835	34.97805807436778	28.354144886939388	20.367564877668997
24	19.98611448327618	24.845844678857294	24.305673999369933	30.86236683849659
25	11.90743256394461	42.87253617357699	20.318002411906228	24.902028850572176
26	15.121568501548074	25.14984117938603	31.249636307817024	28.478954011248874
27	25.155660254313638	29.301048838091265	22.31133656124777	23.23195434634733
28	13.887322643625804	29.58718679832491	34.43006174239156	22.095428815657726
29	21.826146106938545	25.140610922604296	28.44985863661081	24.583384333846347
30	23.32064507455439	33.90152921275942	27.01615896910071	15.761666743585472
31	31.01125489354103	22.77906979077416	20.022232879378603	26.187442436306213
32	24.28520690824523	36.96055670487859	23.67881916923676	15.075417217639423
33	21.72421196682726	25.14602868201966	18.513487211077916	34.61627214007517
34	29.525584867194667	29.583976274226913	22.203583346208873	18.686855512369547
35	22.46905355756169	28.81987153890453	21.70314290243418	27.007932001099604
36	24.845242705588923	33.42817756606155	17.821017294692002	23.90556243365753
37	25.92738998436876	21.107189366744187	35.536689267418595	17.428731381468452
38	26.713767730620972	30.55877165348011	19.07151643086036	23.655944185038557
39	26.14851483161804	25.02944652571128	31.407353304130943	17.414685338539734
40	19.1828814855095	35.84891273594844	25.1275681684562	19.840637610085864
41	27.36991859314834	25.610752045204176	18.043747403990277	28.975581957657198
42	19.19411831985248	27.010941867441474	31.21612646254422	22.57881335016183
43	22.72750074745014	23.428398289593286	20.305160315514257	33.53894064744232
44	25.335850919313508	18.745246919401797	30.428344111999134	25.490558049285557
45	22.039846617211218	17.93318498036564	26.482208680053215	33.544759722369925
46	30.276646848368955	25.62800861223089	25.85595582318841	18.239388716211742
47	13.190638914361278	23.480970621697924	44.05461101490686	19.273779449033935
48	24.16481225457048	25.22508783793274	23.76088819149171	26.849211716005065
49	19.252309069128604	18.779760053455227	40.02339669436413	21.944534183052046
50	24.5348251568642	17.942214579391244	28.016839198893972	29.506121064850582
51	19.767999502368767	17.511201719235654	25.656903329112836	37.06389544928275
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	118.5
2	197.0
3	1296.0
4	2395.0
5	1615.5
6	836.0
7	1025.0
8	1214.0
9	1464.0
10	1714.0
11	1948.5
12	2183.0
13	2177.0
14	2171.0
15	2152.5
16	2134.0
17	1983.5
18	1833.0
19	1854.0
20	1875.0
21	1939.0
22	2003.0
23	2058.5
24	2114.0
25	2461.5
26	3242.0
27	3675.0
28	4017.5
29	4360.0
30	4807.5
31	5255.0
32	6428.5
33	7602.0
34	8061.5
35	8521.0
36	9200.0
37	9879.0
38	11096.5
39	12314.0
40	13597.5
41	14881.0
42	16800.0
43	18719.0
44	22236.5
45	25754.0
46	90824.5
47	155895.0
48	114831.5
49	73768.0
50	64543.5
51	55319.0
52	41414.0
53	27509.0
54	22305.5
55	17102.0
56	14550.5
57	11999.0
58	10141.0
59	8283.0
60	6886.0
61	5489.0
62	4637.0
63	3785.0
64	2982.0
65	2179.0
66	1686.0
67	1193.0
68	928.0
69	663.0
70	498.0
71	333.0
72	263.0
73	193.0
74	145.0
75	70.0
76	43.0
77	26.5
78	10.0
79	13.5
80	17.0
81	11.0
82	5.0
83	3.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	498361.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.70604819520176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.83646959640295	20.73422833396691
2	10.992481403336736	6.091164390914133
3	4.0169207647092415	3.338790009100228
4	2.039195333250888	2.259921767299185
5	1.1880014265576353	1.645741239008714
6	0.8400723992530954	1.396505182869902
7	0.6330173859838208	1.2276887143127864
8	0.5301106191238476	1.1749816289786852
9	0.4645159337911431	1.1582910764150902
>10	4.2466294975613295	21.404608526680736
>50	0.10993298508367248	2.1338813906785594
>100	0.08153969754550543	4.646572617040486
>500	0.006552297124192401	1.2227610350972142
>1k	0.008008363151790711	4.6915537636631655
>5k	0.0036401650689957777	6.779445461830645
>10k+	0.002912132055196622	20.093864862143565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	35181	7.059340518218721	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	29673	5.954117597484554	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	23679	4.751375007273843	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTAGACT	11085	2.224291226640929	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	9668	1.9399591862124044	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTA	6656	1.3355780247651803	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT	6452	1.2946438425157667	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTC	5496	1.1028150276606716	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT	5338	1.0711111021929887	No Hit
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	4150	0.8327296879169919	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	3237	0.6495291565752537	TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	2740	0.5498022517813392	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGT	2530	0.5076641229951783	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATG	2065	0.41435826639725015	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTAT	1572	0.31543399262783406	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1544	0.3098155754563459	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTT	1453	0.29155571964900945	No Hit
TGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCTT	1413	0.2835294094040264	TruSeq Adapter, Index 15 (95% over 24bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGGTAGAC	1325	0.26587152686506366	No Hit
CGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG	1230	0.2468090400332289	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTCGACTCGTATGCCGTCTTCTGC	941	0.18881894851322636	TruSeq Adapter, Index 21 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG	736	0.1476841085076882	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCC	667	0.13383872333509245	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT	663	0.13303609231059413	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGGTAGACTCG	656	0.1316314880177221	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	647	0.1298255682126009	No Hit
TGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTG	629	0.12621372860235852	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	590	0.11838807611350005	No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCT	533	0.1069505840143992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9376736943701454	0.0
2	0.0	0.0	0.0	4.659072439456539	0.0
3	0.0	0.0	0.0	5.6754039742275175	0.0
4	0.0	0.0	0.0	8.24181667506085	0.0
5	0.0	0.0	0.0	15.136216517745169	0.0
6	0.0	0.0	0.0	16.291804535266603	0.0
7	0.0	0.0	0.0	18.346539957982266	0.0
8	0.0	0.0	0.0	20.772692887284517	0.0
9	0.0	0.0	0.0	21.335136577701707	0.0
10	0.0	0.0	0.0	28.808634704561552	0.0
11	0.0	0.0	0.0	31.06563314545079	0.0
12	0.0	0.0	0.0	40.58042262536595	0.0
13	0.0	0.0	0.0	41.632872556239356	0.0
14	0.0	0.0	0.0	42.148161673967266	0.0
15	0.0	0.0	0.0	43.932009125914746	0.0
16	0.0	0.0	0.0	45.04545901465002	0.0
17	0.0	0.0	0.0	46.08045172074059	0.0
18	0.0	0.0	0.0	46.97197413120208	0.0
19	0.0	0.0	0.0	50.23547187681219	0.0
20	0.0	0.0	0.0	51.14786269391064	0.0
21	0.0	0.0	0.0	51.982197643876624	0.0
22	0.0	0.0	0.0	52.965821964399304	0.0
23	0.0	0.0	0.0	53.74457471591878	0.0
24	0.0	0.0	0.0	54.32808747072905	0.0
25	0.0	0.0	0.0	54.85361013401931	0.0
26	0.0	0.0	0.0	55.377126219748334	0.0
27	0.0	0.0	0.0	55.865928513667804	0.0
28	0.0	0.0	0.0	56.29834597811627	0.0
29	0.0	0.0	0.0	56.74621408978632	0.0
30	0.0	0.0	0.0	57.29561502605541	0.0
31	0.0	0.0	0.0	57.76856535724104	0.0
32	0.0	0.0	0.0	58.19235453817614	0.0
33	0.0	0.0	0.0	58.596679916767165	0.0
34	0.0	0.0	0.0	58.97732768013548	0.0
35	0.0	0.0	0.0	59.38787344916637	0.0
36	0.0	0.0	0.0	59.804438950880986	0.0
37	0.0	0.0	0.0	60.19752749512903	0.0
38	0.0	0.0	0.0	60.57295815683811	0.0
39	0.0	0.0	0.0	61.03427033816852	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	135	0.0	45.000004	17
CCCTCGA	70	0.0	45.000004	41
TCTCTCG	45	3.8380676E-10	45.000004	1
GCGCGCG	35	1.2100827E-7	45.000004	1
CGCATGG	35	1.2100827E-7	45.000004	2
CAACATA	35	1.2100827E-7	45.000004	10
CGTTCGG	35	1.2100827E-7	45.000004	2
AGGCACG	45	3.8380676E-10	45.000004	10
CGAGGCA	45	3.8380676E-10	45.000004	34
CTTCTCG	35	1.2100827E-7	45.000004	1
CTTTCCG	35	1.2100827E-7	45.000004	1
CCGGTGA	115	0.0	45.000004	18
TTGTTCG	45	3.8380676E-10	45.000004	1
CGACTGA	35	1.2100827E-7	45.000004	41
TCACGGG	20	7.029437E-4	45.0	3
CTTGTCG	25	3.887615E-5	45.0	1
AACGTAG	20	7.029437E-4	45.0	45
CGGGTAT	25	3.887615E-5	45.0	6
CGGGTAC	20	7.029437E-4	45.0	6
TCGTTCG	20	7.029437E-4	45.0	1
>>END_MODULE