SRR2933835.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933835.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351805
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	27932	7.939625644888504	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	22036	6.263697218629638	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	19199	5.4572845752618635	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	9552	2.7151404897599525	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCTAGAT	9041	2.5698895695058344	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	5961	1.6944045707138897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTA	5866	1.6674009749719305	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	4710	1.3388098520487202	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	4288	1.2188570372791745	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	2915	0.8285840167138045	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2869	0.8155085914071716	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2441	0.693850286380239	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGT	2119	0.6023223092338086	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATG	1861	0.5289862281661716	No Hit
TGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCTT	1583	0.44996517957391163	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTAT	1364	0.38771478517928964	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	1196	0.33996105797245635	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCTCTAGA	1062	0.3018717755574821	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	763	0.21688151106436807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.20352183738150398	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCTCTAGATCG	588	0.16713804522391665	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	570	0.16202157445175594	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	556	0.15804209718451984	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	542	0.15406261991728373	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	538	0.15292562641235913	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	445	0.1264905274228621	No Hit
GCACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	394	0.11199386023507339	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT	369	0.10488765082929463	No Hit
TGCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	360	0.10232941544321428	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	357	0.10147667031452083	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAT	50	2.1827873E-11	45.000004	25
CGCGGGC	25	3.8852682E-5	45.000004	4
CGTGCGG	25	3.8852682E-5	45.000004	2
TGGACCC	25	3.8852682E-5	45.000004	37
GTTTACG	25	3.8852682E-5	45.000004	1
TTTCGCG	50	2.1827873E-11	45.000004	1
ATCAGTG	25	3.8852682E-5	45.000004	38
CATTGGT	25	3.8852682E-5	45.000004	28
GCTATAC	25	3.8852682E-5	45.000004	33
GGCCGAT	25	3.8852682E-5	45.000004	8
ACCGAGG	60	0.0	45.000004	17
GACCACT	30	2.1611922E-6	45.000004	13
CCTCCGC	30	2.1611922E-6	45.000004	45
CGGGCTT	50	2.1827873E-11	45.000004	6
GTCCCGT	30	2.1611922E-6	45.000004	27
CGACTTG	25	3.8852682E-5	45.000004	19
TCGACTT	25	3.8852682E-5	45.000004	18
TGTAATC	25	3.8852682E-5	45.000004	24
TGTCGTG	25	3.8852682E-5	45.000004	1
GCCAACC	30	2.1611922E-6	45.000004	16

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content