##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933835.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351805
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.92211025994514	25.0	16.0	31.0	16.0	33.0
2	24.736103807507	28.0	16.0	31.0	16.0	34.0
3	25.861750117252456	30.0	16.0	33.0	16.0	34.0
4	30.546623839911316	32.0	28.0	35.0	19.0	37.0
5	33.03922911840366	35.0	32.0	35.0	28.0	37.0
6	31.02835661801282	35.0	28.0	35.0	17.0	37.0
7	33.02610821335683	35.0	32.0	35.0	28.0	37.0
8	31.581549437898836	35.0	28.0	36.0	17.0	37.0
9	34.04056792825571	35.0	32.0	37.0	28.0	39.0
10	33.59420986057617	35.0	32.0	37.0	28.0	39.0
11	34.33345461264052	35.0	32.0	39.0	27.0	39.0
12	34.47578914455451	37.0	34.0	39.0	27.0	39.0
13	34.68119554867043	35.0	34.0	38.0	30.0	39.0
14	34.42013331248845	36.0	32.0	38.0	27.0	40.0
15	35.06054490413724	37.0	33.0	39.0	27.0	40.0
16	34.55015988971163	36.0	32.0	38.0	27.0	40.0
17	34.02792456048095	36.0	32.0	38.0	27.0	40.0
18	34.36029050184051	36.0	32.0	38.0	27.0	40.0
19	34.060974119185346	36.0	32.0	39.0	27.0	40.0
20	33.78126518952261	36.0	31.0	39.0	25.0	40.0
21	33.85651426216228	36.0	32.0	39.0	25.0	40.0
22	35.14601554838618	37.0	33.0	39.0	28.0	40.0
23	35.38724009039098	37.0	33.0	39.0	29.0	40.0
24	35.6663322010773	37.0	34.0	39.0	30.0	40.0
25	33.794397464504485	36.0	32.0	39.0	24.0	40.0
26	34.82823154872728	37.0	33.0	39.0	27.0	40.0
27	34.91570898651241	37.0	33.0	39.0	27.0	40.0
28	35.01832833529939	37.0	33.0	39.0	27.0	40.0
29	35.52155881809525	38.0	34.0	40.0	27.0	40.0
30	33.9575759298475	37.0	32.0	39.0	24.0	40.0
31	34.54149031423658	37.0	33.0	39.0	25.0	40.0
32	34.79328605335342	37.0	33.0	39.0	27.0	40.0
33	35.04565313170649	37.0	34.0	39.0	27.0	40.0
34	35.2470999559415	38.0	34.0	40.0	27.0	40.0
35	35.49457796222339	38.0	35.0	40.0	29.0	40.0
36	35.521058540953085	38.0	35.0	40.0	28.0	41.0
37	36.043129006125554	38.0	35.0	40.0	30.0	41.0
38	35.44398175125425	38.0	34.0	40.0	30.0	40.0
39	35.44536035587897	38.0	34.0	40.0	29.0	40.0
40	35.09554156421881	37.0	33.0	40.0	28.0	40.0
41	34.05204872017168	37.0	32.0	39.0	24.0	40.0
42	35.302895069711916	38.0	34.0	40.0	29.0	40.0
43	35.497113457739374	38.0	35.0	40.0	30.0	40.0
44	36.078097241369505	38.0	35.0	40.0	31.0	41.0
45	36.265823396483846	38.0	35.0	40.0	31.0	41.0
46	36.022151475959696	38.0	35.0	40.0	30.0	41.0
47	35.307582325435966	38.0	34.0	40.0	28.0	41.0
48	35.62483193814755	38.0	35.0	40.0	29.0	41.0
49	36.074305368030586	38.0	35.0	40.0	30.0	41.0
50	35.76373843464419	38.0	35.0	40.0	29.0	41.0
51	35.37534145336195	38.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	11.0
11	5.0
12	14.0
13	12.0
14	14.0
15	23.0
16	56.0
17	167.0
18	456.0
19	876.0
20	1316.0
21	1819.0
22	1999.0
23	2160.0
24	2357.0
25	2986.0
26	4248.0
27	5644.0
28	7693.0
29	10424.0
30	14046.0
31	19158.0
32	26462.0
33	34577.0
34	43122.0
35	51172.0
36	52078.0
37	43934.0
38	22799.0
39	2174.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.54187689202825	2.953909125794119	32.00636716362758	11.497846818550048
2	13.93868762524694	39.077329770753686	29.671551001264906	17.312431602734467
3	12.225238413325563	39.220306703997956	33.96995494663237	14.584499936044114
4	14.313042736743368	2.965279060843365	64.98287403533207	17.738804167081195
5	40.02359261522719	3.302966131805972	34.729182359545774	21.944258893421072
6	14.974488708233254	31.526555904549397	37.55915919330311	15.939796193914244
7	50.394963118773184	1.3680874348005287	42.202072170662724	6.034877275763562
8	43.87544236153551	27.142593197936353	16.46281320617956	12.519151234348575
9	41.23619618822927	3.7566265402708887	16.94717243927744	38.0600048322224
10	39.266639189323634	16.31983627293529	25.11305979164594	19.300464746095138
11	32.77497477295661	18.250166995921035	28.697431815920755	20.277426415201603
12	20.132175494947486	14.283480905615326	32.71727235258168	32.8670712468555
13	20.916701013345463	21.511064368044796	44.346441920950525	13.225792697659214
14	18.415315302511335	32.31392390670968	23.86577791674365	25.40498287403533
15	8.819942866076378	23.06277625389065	46.300081010787224	21.817199869245744
16	9.798041528687767	24.811188016088458	24.141498841687866	41.24927161353591
17	11.059820070777846	29.48025184406134	35.80563096033314	23.654297124827675
18	10.053580818919572	24.021830275294555	34.49695143616492	31.427637469620954
19	11.457199300748995	27.67527465499353	29.01863248106195	31.848893563195517
20	14.507468626085473	25.19435482724805	45.41976378959936	14.878412757067125
21	18.749875641335397	36.38805588323077	26.8984238427538	17.963644632680037
22	12.352297437500887	21.44568724151163	36.10750273589062	30.094512585096854
23	15.613763306377113	34.004064751780106	28.355196770938445	22.026975170904336
24	21.243018149258823	22.489163030656186	23.460155483861797	32.80766333622319
25	10.567501883145493	40.472705049672406	20.333139096942908	28.626653970239197
26	13.929875925583776	20.129333011185174	30.13970807691761	35.801082986313446
27	20.07703130995864	27.67015818422137	21.60088685493384	30.651923650886143
28	8.794076263839342	24.211708190616964	38.45994229757962	28.53427324796407
29	17.05035459984935	15.817569392134848	24.404712838077913	42.727363169937895
30	12.860817782578419	28.702548286692913	32.63228208808857	25.8043518426401
31	19.68391580563096	22.441977800201816	25.707423146345278	32.16668324782195
32	23.66339307286707	29.306576086184105	19.623370901493725	27.406659939455096
33	13.61748696010574	22.66227029178096	26.509856312445816	37.21038643566749
34	23.83564758886315	26.104233879563964	22.708602777106634	27.351515754466256
35	20.09095948039397	20.589246883927174	20.741319765210843	38.57847387046802
36	15.310470288938474	26.707977430678927	27.852077145009307	30.12947513537329
37	13.629425391907448	19.78795071133156	31.194838049487643	35.38778584727334
38	18.319239351345207	21.528687767371128	34.129702534074276	26.02237034720939
39	18.25130398942596	28.434217819530705	25.73897471610693	27.575503474936397
40	22.71201375762141	18.961356433251375	34.68825059336849	23.638379215758732
41	16.938076491238043	38.83742414121459	20.388283281931752	23.83621608561561
42	26.306902971816776	16.96110060971277	32.22353292306818	24.50846349540228
43	22.283367206264835	25.57467915464533	22.05227327638891	30.08968036270093
44	19.176248205682125	17.665468086013558	32.10926507582326	31.04901863248106
45	17.942041756086468	14.084791290629752	30.418555734000368	37.554611219283416
46	30.444422336237402	22.952772132289194	24.84814030499851	21.75466522647489
47	11.298872955188244	16.656954847145435	50.17154389505551	21.87262830261082
48	19.648953255354527	25.866317988658487	25.20003979477267	29.28468896121431
49	20.31437870411165	13.132843478631628	42.89904918918151	23.653728628075214
50	24.896178280581573	16.71693125453021	30.321911286081775	28.064979178806443
51	18.714344594306507	14.487571239749292	27.088301758076206	39.70978240786799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	121.0
2	182.0
3	718.5
4	1255.0
5	919.0
6	583.0
7	774.5
8	966.0
9	1114.0
10	1262.0
11	1282.0
12	1302.0
13	1289.5
14	1277.0
15	1124.5
16	972.0
17	924.0
18	876.0
19	969.0
20	1062.0
21	1087.0
22	1112.0
23	1261.5
24	1411.0
25	1563.0
26	1874.0
27	2033.0
28	2441.0
29	2849.0
30	2943.5
31	3038.0
32	3169.0
33	3300.0
34	3774.0
35	4248.0
36	4943.0
37	5638.0
38	6116.0
39	6594.0
40	7345.5
41	8097.0
42	9477.5
43	10858.0
44	13721.5
45	16585.0
46	67894.0
47	119203.0
48	86618.0
49	54033.0
50	48397.0
51	42761.0
52	30457.5
53	18154.0
54	15046.0
55	11938.0
56	10179.5
57	8421.0
58	7364.0
59	6307.0
60	5486.5
61	4666.0
62	4078.0
63	3490.0
64	2826.5
65	2163.0
66	1686.0
67	1209.0
68	1031.5
69	854.0
70	705.0
71	556.0
72	466.5
73	377.0
74	272.0
75	123.0
76	79.0
77	74.0
78	69.0
79	61.5
80	54.0
81	33.0
82	12.0
83	8.0
84	4.0
85	8.0
86	12.0
87	6.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	351805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.926862892795725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86186028154191	19.40933187419167
2	10.62908389247029	5.511576015122014
3	4.048809367188528	3.1491877602649194
4	2.0446871025742226	2.1204928866843846
5	1.2147524448537472	1.5747360043205754
6	0.870499495680393	1.3541592643652023
7	0.6709643467964741	1.21772004377425
8	0.5854492829890804	1.2143090632594762
9	0.5481734859448318	1.2791176930401784
>10	4.26917510853835	18.78171145947329
>50	0.13375433057053895	2.4675601540626197
>100	0.09538218655440073	4.891630306561874
>500	0.0076744288032276455	1.2145933116357073
>1k	0.0120598166907863	7.50643111951223
>5k	0.004385387887558654	8.646835604951606
>10k+	0.003289040915668991	19.660607438780005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	27932	7.939625644888504	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	22036	6.263697218629638	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTC	19199	5.4572845752618635	No Hit
GCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	9552	2.7151404897599525	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCTCTAGAT	9041	2.5698895695058344	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	5961	1.6944045707138897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTA	5866	1.6674009749719305	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTC	4710	1.3388098520487202	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCT	4288	1.2188570372791745	No Hit
CTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCT	2915	0.8285840167138045	Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2869	0.8155085914071716	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGC	2441	0.693850286380239	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGT	2119	0.6023223092338086	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATG	1861	0.5289862281661716	No Hit
TGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTGCTT	1583	0.44996517957391163	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTAT	1364	0.38771478517928964	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTT	1196	0.33996105797245635	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCTCTAGA	1062	0.3018717755574821	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	763	0.21688151106436807	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	716	0.20352183738150398	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCTCTAGATCG	588	0.16713804522391665	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	570	0.16202157445175594	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	556	0.15804209718451984	No Hit
TGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCTG	542	0.15406261991728373	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	538	0.15292562641235913	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGC	445	0.1264905274228621	No Hit
GCACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	394	0.11199386023507339	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGT	369	0.10488765082929463	No Hit
TGCCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCGTCTTCT	360	0.10232941544321428	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCTCTAGATCGTATGCCG	357	0.10147667031452083	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9812253947499325	0.0
2	0.0	0.0	0.0	5.501627321953923	0.0
3	0.0	0.0	0.0	6.598541805829934	0.0
4	0.0	0.0	0.0	9.95921035801083	0.0
5	0.0	0.0	0.0	17.899404499651794	0.0
6	0.0	0.0	0.0	19.306433961995992	0.0
7	0.0	0.0	0.0	21.521013061212887	0.0
8	0.0	0.0	0.0	24.24155427012123	0.0
9	0.0	0.0	0.0	24.768266511277556	0.0
10	0.0	0.0	0.0	32.548144568724155	0.0
11	0.0	0.0	0.0	34.75561745853527	0.0
12	0.0	0.0	0.0	45.066443057944035	0.0
13	0.0	0.0	0.0	46.17188499310698	0.0
14	0.0	0.0	0.0	46.76965932832109	0.0
15	0.0	0.0	0.0	48.93676894870738	0.0
16	0.0	0.0	0.0	50.22981481218289	0.0
17	0.0	0.0	0.0	51.19227981410156	0.0
18	0.0	0.0	0.0	52.03337075936954	0.0
19	0.0	0.0	0.0	55.57368428532852	0.0
20	0.0	0.0	0.0	56.49209078893137	0.0
21	0.0	0.0	0.0	57.269510097923565	0.0
22	0.0	0.0	0.0	58.169156208695156	0.0
23	0.0	0.0	0.0	58.83912963147198	0.0
24	0.0	0.0	0.0	59.37891729793493	0.0
25	0.0	0.0	0.0	59.8257557453703	0.0
26	0.0	0.0	0.0	60.28879635025085	0.0
27	0.0	0.0	0.0	60.751836955131395	0.0
28	0.0	0.0	0.0	61.153195662369775	0.0
29	0.0	0.0	0.0	61.56393456602379	0.0
30	0.0	0.0	0.0	62.05056778613152	0.0
31	0.0	0.0	0.0	62.49740623356689	0.0
32	0.0	0.0	0.0	62.86863461292477	0.0
33	0.0	0.0	0.0	63.222808089708785	0.0
34	0.0	0.0	0.0	63.59403646906667	0.0
35	0.0	0.0	0.0	63.97464504484018	0.0
36	0.0	0.0	0.0	64.35383237873253	0.0
37	0.0	0.0	0.0	64.69805716234846	0.0
38	0.0	0.0	0.0	65.0360284816873	0.0
39	0.0	0.0	0.0	65.40043490001564	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAAT	50	2.1827873E-11	45.000004	25
CGCGGGC	25	3.8852682E-5	45.000004	4
CGTGCGG	25	3.8852682E-5	45.000004	2
TGGACCC	25	3.8852682E-5	45.000004	37
GTTTACG	25	3.8852682E-5	45.000004	1
TTTCGCG	50	2.1827873E-11	45.000004	1
ATCAGTG	25	3.8852682E-5	45.000004	38
CATTGGT	25	3.8852682E-5	45.000004	28
GCTATAC	25	3.8852682E-5	45.000004	33
GGCCGAT	25	3.8852682E-5	45.000004	8
ACCGAGG	60	0.0	45.000004	17
GACCACT	30	2.1611922E-6	45.000004	13
CCTCCGC	30	2.1611922E-6	45.000004	45
CGGGCTT	50	2.1827873E-11	45.000004	6
GTCCCGT	30	2.1611922E-6	45.000004	27
CGACTTG	25	3.8852682E-5	45.000004	19
TCGACTT	25	3.8852682E-5	45.000004	18
TGTAATC	25	3.8852682E-5	45.000004	24
TGTCGTG	25	3.8852682E-5	45.000004	1
GCCAACC	30	2.1611922E-6	45.000004	16
>>END_MODULE