FastQCFastQC Report
Sat 14 Jan 2017
SRR2933834.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933834.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1093967
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC536664.905632436810251No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG429243.923701537614937No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC348683.1872990684362508RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT159311.4562596495141078No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC113151.0343090787930533RNA PCR Primer, Index 48 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT111251.016941096029405TruSeq Adapter, Index 4 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA104810.9580727755041971No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC80100.7321975891411715No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT65550.599195405345865TruSeq Adapter, Index 4 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.4004691183554897No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGT37470.3425149021862634No Hit
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT34680.3170113906543799TruSeq Adapter, Index 4 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATG34380.3142690775864354No Hit
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC33790.30887586188614463RNA PCR Primer, Index 4 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT23000.21024400187574216TruSeq Adapter, Index 4 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTAT22660.2071360470654051No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC19370.17706201375361413RNA PCR Primer, Index 48 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGCAATC17270.1578658222780029No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG14060.12852307245099717TruSeq Adapter, Index 4 (96% over 25bp)
TGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCTT12060.1102409853313674RNA PCR Primer, Index 48 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCGCGCAATCTCGTATGCCGTCTTC11310.10338520266150623RNA PCR Primer, Index 32 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA207.033138E-445.09
CCTAGCG207.033138E-445.045
TATGCGA207.033138E-445.030
TACGGCT62800.043.638547
ACGGCTG62950.043.5702938
TGATACC51150.043.5483864
TTATACG1400.043.39285726
TATACGG1350.043.33333227
GATACCT51700.043.2156685
CGGCTGT66600.041.858119
CGTTTTT25200.041.696431
AATGATC12350.041.5384642
CACCGGT3600.041.2516
ATACCTG54500.041.1605536
GAATCTG41450.040.6031381
GCGACTG13000.040.32692311
ATGATAC204900.040.322113
AATGATA205300.039.8928382
GAATGAT213200.039.7971341
ACCACCT22750.039.65934414
Produced by FastQC (version 0.11.5)