##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933834.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1093967
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.692414853464502	30.0	16.0	33.0	16.0	34.0
2	26.726573105038817	31.0	16.0	33.0	16.0	34.0
3	27.794149183659105	31.0	25.0	34.0	16.0	34.0
4	31.68293650539733	35.0	28.0	35.0	19.0	37.0
5	33.39119187324663	35.0	33.0	37.0	28.0	37.0
6	32.07963951380618	35.0	32.0	37.0	17.0	37.0
7	33.10666500909077	35.0	32.0	35.0	28.0	37.0
8	32.55913478194498	35.0	32.0	37.0	25.0	37.0
9	34.28211362865608	35.0	32.0	39.0	28.0	39.0
10	33.323711775583725	35.0	31.0	37.0	27.0	39.0
11	33.85583477380945	35.0	32.0	39.0	27.0	39.0
12	34.05710409911816	35.0	32.0	39.0	26.0	39.0
13	34.22590352359806	35.0	33.0	38.0	27.0	39.0
14	34.24495345837671	36.0	32.0	39.0	25.0	40.0
15	34.719834327726524	36.0	32.0	39.0	27.0	40.0
16	34.648277324635934	36.0	32.0	39.0	27.0	40.0
17	34.07814678139286	36.0	32.0	38.0	26.0	40.0
18	34.37182840067388	36.0	32.0	39.0	27.0	40.0
19	34.17744136706135	36.0	32.0	39.0	27.0	40.0
20	34.065643662011745	36.0	32.0	39.0	25.0	40.0
21	33.83825746114828	36.0	32.0	39.0	25.0	40.0
22	34.81870294076512	37.0	33.0	39.0	27.0	40.0
23	35.42913725916778	37.0	34.0	39.0	29.0	40.0
24	35.71754358221043	38.0	34.0	39.0	30.0	40.0
25	33.72771847779686	36.0	32.0	39.0	24.0	40.0
26	34.60170645001175	37.0	33.0	39.0	27.0	40.0
27	34.87773762828312	37.0	33.0	39.0	27.0	40.0
28	35.02544135243568	37.0	33.0	39.0	27.0	40.0
29	35.431186681133894	38.0	34.0	40.0	27.0	40.0
30	32.98018404577103	36.0	30.0	39.0	21.0	40.0
31	34.10335503721776	36.0	32.0	39.0	25.0	40.0
32	34.490979161163	37.0	33.0	39.0	26.0	40.0
33	34.9309083363575	37.0	33.0	39.0	26.0	40.0
34	35.09783841742941	38.0	34.0	40.0	27.0	40.0
35	35.24741148498995	38.0	34.0	40.0	27.0	41.0
36	35.2879026515425	38.0	34.0	40.0	27.0	41.0
37	35.308204909288854	38.0	34.0	40.0	27.0	41.0
38	35.026593123924215	37.0	34.0	40.0	27.0	41.0
39	34.96993876414919	37.0	34.0	40.0	27.0	41.0
40	34.48782275882179	37.0	33.0	40.0	25.0	41.0
41	33.81427319105604	37.0	32.0	39.0	23.0	40.0
42	34.902105822204874	37.0	34.0	40.0	27.0	41.0
43	35.05786737625541	37.0	34.0	40.0	27.0	41.0
44	35.61251390581251	38.0	35.0	40.0	29.0	41.0
45	35.913577831872445	38.0	35.0	40.0	30.0	41.0
46	35.54367910549404	38.0	35.0	40.0	29.0	41.0
47	35.092869346150295	37.0	34.0	40.0	26.0	41.0
48	35.26301067582477	37.0	34.0	40.0	28.0	41.0
49	35.511693679973895	38.0	34.0	40.0	29.0	41.0
50	35.24405855021221	37.0	34.0	40.0	28.0	41.0
51	34.739161236125035	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	6.0
10	15.0
11	23.0
12	24.0
13	29.0
14	62.0
15	119.0
16	312.0
17	940.0
18	2057.0
19	3727.0
20	5529.0
21	6839.0
22	7297.0
23	7675.0
24	9122.0
25	11447.0
26	14894.0
27	19436.0
28	24837.0
29	33154.0
30	45159.0
31	61104.0
32	79768.0
33	99312.0
34	115318.0
35	136829.0
36	151455.0
37	150861.0
38	95209.0
39	11404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.673103484840034	3.3427882193887015	40.34061356512582	14.64349473064544
2	15.98311466433631	26.321909161793727	40.36502015143053	17.329956022439433
3	15.98558274609746	26.324194422683682	41.992674367691166	15.697548463527694
4	15.50906014532431	3.3053099407934603	61.324518929730054	19.861110984152173
5	32.8758545733098	4.188151927800381	41.31221508509854	21.623778413791275
6	17.8451452374706	22.615490229595594	44.057087645239754	15.482276887694052
7	65.57464713286598	1.6532491382281183	28.757631628742004	4.014472100163899
8	63.38646412551749	18.63264614014865	11.19494463726968	6.785945097064171
9	59.730595164205134	3.803862456545764	11.265422083115853	25.20012029613325
10	41.875029137076346	24.48401094365735	18.209415823329223	15.431544095937081
11	34.45661523610858	19.178092209362806	27.660249349386223	18.705043205142385
12	25.42224765463675	16.517134429100693	29.336076865207083	28.724541051055468
13	26.589376096353913	18.380079106590966	38.44759485432376	16.58294994273136
14	20.237813389252143	27.4243190151074	28.415756599604926	23.922110996035528
15	13.856816521887772	21.50494484751368	42.01708095399587	22.621157676602678
16	18.369749727368376	23.913975467267296	25.181929619449217	32.534345185915114
17	19.99914074190538	26.70537593912796	32.761134476634126	20.53434884233254
18	18.565185238677216	23.194666749545462	30.95440721703671	27.285740794740608
19	18.61893457480893	25.88908074923649	28.295186235050966	27.196798440903606
20	20.47164128351221	26.4222778200805	37.552412458511085	15.553668437896206
21	21.665735803730826	31.825822899593863	29.158100747097492	17.35034054957782
22	18.4179230269286	19.487607944298137	36.9840223699618	25.110446658811465
23	20.269258579097908	29.152707531397198	31.12104844113214	19.45698544837276
24	21.64471140354325	24.247349325893744	26.299147963329787	27.808791307233214
25	17.641665607829122	35.177386520799985	23.274193828515852	23.90675404285504
26	19.285042419012637	21.95148482541064	30.46901780401054	28.294454951566177
27	22.05642400547731	27.683924652206144	25.91897196167709	24.34067938063945
28	16.98945214983633	26.333244055807896	32.599246595189804	24.078057199165972
29	24.60055924904499	22.177360011773665	26.20563508771288	27.016445651468462
30	19.924732647328483	33.003189310098016	28.27324773050741	18.798830312066087
31	27.172757496341298	22.57508681706121	23.50290273838242	26.749252948215073
32	25.863211595962216	29.215506500653127	22.78441671458097	22.136865188803682
33	20.98244279763466	25.91184194770043	22.053407461102577	31.052307793562328
34	27.813179008141926	25.58623797609983	25.676734307342	20.92384870841625
35	18.453664507247478	27.53995321613906	27.235830696904017	26.770551579709444
36	29.166693328043714	25.67444904645204	21.530539769481162	23.628317856023077
37	17.840026253077106	29.296496146593086	28.303138942948003	24.560338657381802
38	26.364872066524857	26.393117891124685	22.08576675530432	25.156243287046138
39	21.11160574313485	27.478068351239116	24.114804194276427	27.295521711349615
40	19.47508471462119	28.104595476828827	30.822410548032984	21.597909260517
41	19.23558937335404	29.98664493535911	22.911111578320003	27.86665411296684
42	18.60805673297275	19.929120348237195	37.43211632526393	24.03070659352613
43	23.18872507123158	20.848983561661367	23.88847195573541	32.07381941137164
44	21.60229696142571	22.920344032315416	29.892035134515027	25.58532387174385
45	21.274773370677543	18.82515651751835	27.70467482108693	32.19539529071718
46	27.397170115734752	24.28345644795501	24.889233404663944	23.430140031646292
47	15.1738580779859	23.57959609384927	39.8725007244277	21.37404510373713
48	20.824485564921062	28.34875275031148	25.317582705876866	25.50917897889059
49	20.086986170515196	19.3758129815616	37.52773164089959	23.00946920702361
50	23.126200333282448	20.280867704418874	28.394183736803765	28.198748225494917
51	20.036619020500616	20.27538307828298	26.666800735305547	33.021197165910856
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	115.0
1	442.5
2	770.0
3	4232.0
4	7694.0
5	5273.0
6	2852.0
7	2791.5
8	2731.0
9	2809.5
10	2888.0
11	2838.5
12	2789.0
13	2691.0
14	2593.0
15	2420.0
16	2247.0
17	2232.5
18	2218.0
19	2262.5
20	2307.0
21	2600.5
22	2894.0
23	3427.5
24	3961.0
25	4850.0
26	6802.5
27	7866.0
28	9687.5
29	11509.0
30	12908.0
31	14307.0
32	16598.5
33	18890.0
34	21220.5
35	23551.0
36	25606.0
37	27661.0
38	29848.0
39	32035.0
40	34234.0
41	36433.0
42	39871.5
43	43310.0
44	49348.5
45	55387.0
46	150421.0
47	245455.0
48	184557.5
49	123660.0
50	113577.5
51	103495.0
52	86163.5
53	68832.0
54	62459.0
55	56086.0
56	52445.0
57	48804.0
58	44722.5
59	40641.0
60	35722.5
61	30804.0
62	26281.0
63	21758.0
64	18176.5
65	14595.0
66	12169.5
67	9744.0
68	8416.5
69	7089.0
70	5940.0
71	4791.0
72	3720.5
73	2650.0
74	2044.5
75	1077.5
76	716.0
77	533.0
78	350.0
79	257.0
80	164.0
81	132.5
82	101.0
83	57.5
84	14.0
85	22.0
86	30.0
87	16.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1093967.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.337762404958116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.27112406467299	21.046774672938703
2	9.80026690928467	5.554352703609645
3	3.4732689363898657	2.952740096638128
4	1.7342094342886194	1.965744596372309
5	1.153701197579925	1.6346655211667775
6	0.8223537369437137	1.3982198886204231
7	0.7113522857911921	1.4110692442682267
8	0.6317725943437719	1.4322417337982234
9	0.5798057939902198	1.4787358948001648
>10	6.672620153730965	34.475975103597214
>50	0.08670483160326506	1.6656745084646478
>100	0.05005973641283191	2.8819444855051217
>500	0.005235005115063855	1.0214709776018687
>1k	0.004580629475680873	3.012027428371998
>5k	6.543756393829819E-4	1.3504334019035324
>10k+	0.0022903147378404366	16.717929742343006
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	53666	4.905632436810251	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	42924	3.923701537614937	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	34868	3.1872990684362508	RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT	15931	1.4562596495141078	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	11315	1.0343090787930533	RNA PCR Primer, Index 48 (96% over 25bp)
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	11125	1.016941096029405	TruSeq Adapter, Index 4 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA	10481	0.9580727755041971	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC	8010	0.7321975891411715	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT	6555	0.599195405345865	TruSeq Adapter, Index 4 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4381	0.4004691183554897	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGT	3747	0.3425149021862634	No Hit
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	3468	0.3170113906543799	TruSeq Adapter, Index 4 (96% over 27bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATG	3438	0.3142690775864354	No Hit
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	3379	0.30887586188614463	RNA PCR Primer, Index 4 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT	2300	0.21024400187574216	TruSeq Adapter, Index 4 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTAT	2266	0.2071360470654051	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	1937	0.17706201375361413	RNA PCR Primer, Index 48 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGAGCAATC	1727	0.1578658222780029	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG	1406	0.12852307245099717	TruSeq Adapter, Index 4 (96% over 25bp)
TGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCTT	1206	0.1102409853313674	RNA PCR Primer, Index 48 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCGCGCAATCTCGTATGCCGTCTTC	1131	0.10338520266150623	RNA PCR Primer, Index 32 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3918765374092637	0.0
2	0.0	0.0	0.0	2.1669757862897145	0.0
3	9.141043559814876E-5	0.0	0.0	2.599529967540154	0.0
4	9.141043559814876E-5	0.0	0.0	4.229926496868735	0.0
5	9.141043559814876E-5	0.0	0.0	8.617718816015474	0.0
6	9.141043559814876E-5	0.0	0.0	9.28995115940426	0.0
7	9.141043559814876E-5	0.0	0.0	10.43258160438112	0.0
8	9.141043559814876E-5	0.0	0.0	11.8902124104292	0.0
9	9.141043559814876E-5	0.0	0.0	12.25164927278428	0.0
10	9.141043559814876E-5	0.0	0.0	17.12620216149116	0.0
11	9.141043559814876E-5	0.0	0.0	18.330900292239164	0.0
12	9.141043559814876E-5	0.0	0.0	24.903310611746058	0.0
13	9.141043559814876E-5	0.0	0.0	25.6072623762874	0.0
14	9.141043559814876E-5	0.0	0.0	25.96138640379463	0.0
15	9.141043559814876E-5	0.0	0.0	27.252741627489677	0.0
16	9.141043559814876E-5	0.0	0.0	27.987315887956402	0.0
17	9.141043559814876E-5	0.0	0.0	28.595012463812893	0.0
18	9.141043559814876E-5	0.0	0.0	29.118245797176698	0.0
19	9.141043559814876E-5	0.0	0.0	31.260357944983717	0.0
20	9.141043559814876E-5	0.0	0.0	31.933961444906473	0.0
21	9.141043559814876E-5	0.0	0.0	32.627858061532024	0.0
22	9.141043559814876E-5	0.0	0.0	33.44433607229468	0.0
23	9.141043559814876E-5	0.0	0.0	34.065835623926496	0.0
24	9.141043559814876E-5	0.0	0.0	34.590988576437866	0.0
25	9.141043559814876E-5	0.0	0.0	35.06129526759034	0.0
26	9.141043559814876E-5	0.0	0.0	35.554820209384744	0.0
27	9.141043559814876E-5	0.0	0.0	36.07247750617706	0.0
28	9.141043559814876E-5	0.0	0.0	36.49141153252338	0.0
29	9.141043559814876E-5	0.0	0.0	36.963455021952214	0.0
30	9.141043559814876E-5	0.0	0.0	37.552778100253484	0.0
31	9.141043559814876E-5	0.0	0.0	38.00206039121838	0.0
32	1.8282087119629752E-4	0.0	0.0	38.42026313407991	0.0
33	1.8282087119629752E-4	0.0	0.0	38.854188471864326	0.0
34	1.8282087119629752E-4	0.0	0.0	39.327146065649146	0.0
35	1.8282087119629752E-4	0.0	0.0	39.83228013276452	0.0
36	1.8282087119629752E-4	0.0	0.0	40.28777833335009	0.0
37	1.8282087119629752E-4	0.0	0.0	40.74501333221203	0.0
38	1.8282087119629752E-4	0.0	0.0	41.29109927447537	0.0
39	1.8282087119629752E-4	0.0	0.0	42.31407345925425	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTA	20	7.033138E-4	45.0	9
CCTAGCG	20	7.033138E-4	45.0	45
TATGCGA	20	7.033138E-4	45.0	30
TACGGCT	6280	0.0	43.63854	7
ACGGCTG	6295	0.0	43.570293	8
TGATACC	5115	0.0	43.548386	4
TTATACG	140	0.0	43.392857	26
TATACGG	135	0.0	43.333332	27
GATACCT	5170	0.0	43.215668	5
CGGCTGT	6660	0.0	41.85811	9
CGTTTTT	2520	0.0	41.69643	1
AATGATC	1235	0.0	41.538464	2
CACCGGT	360	0.0	41.25	16
ATACCTG	5450	0.0	41.160553	6
GAATCTG	4145	0.0	40.603138	1
GCGACTG	1300	0.0	40.326923	11
ATGATAC	20490	0.0	40.32211	3
AATGATA	20530	0.0	39.892838	2
GAATGAT	21320	0.0	39.797134	1
ACCACCT	2275	0.0	39.659344	14
>>END_MODULE