SRR2933833.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933833.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421899
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	25964	6.154079530882984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	24073	5.705867992102375	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	19872	4.7101320458213936	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	9284	2.2005266663348335	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	6149	1.457457827584327	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTA	6148	1.4572208040312966	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	5884	1.394646586031254	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	4989	1.182510506068988	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	4195	0.9943138049627992	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	2450	0.5807077049246384	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGT	2205	0.5226369344321745	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	1931	0.45769248090182724	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATG	1866	0.442285949954847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1592	0.3773414964244997	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	1253	0.2969905119471722	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1180	0.2796877925759483	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	1104	0.26167400254563294	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCTCAT	926	0.21948381010621024	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	869	0.20597346758347376	No Hit
TGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCTT	832	0.19720359612134655	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTGCTCATTCG	536	0.12704462442432904	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	478	0.11329725834856211	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	460	0.10903083439401373	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	452	0.10713464596977001	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	115	0.0	45.000004	1
ATCCGTC	35	1.2094824E-7	45.000004	6
GCGATAC	35	1.2094824E-7	45.000004	33
GTACGGG	30	2.1622218E-6	45.000004	3
CGGGTAT	20	7.028205E-4	45.0	6
CGCGGGC	90	0.0	45.0	4
CCGATCG	25	3.886594E-5	45.0	40
CCGATCA	20	7.028205E-4	45.0	40
GCGAACA	20	7.028205E-4	45.0	33
GGGTACG	40	6.7975634E-9	45.0	7
CTCCCGG	20	7.028205E-4	45.0	2
CGATTGT	40	6.7975634E-9	45.0	10
CCTCGAA	25	3.886594E-5	45.0	42
TGTTGCG	20	7.028205E-4	45.0	1
TTGCGCG	20	7.028205E-4	45.0	1
GAGCATT	20	7.028205E-4	45.0	34
TCCCCCG	20	7.028205E-4	45.0	1
GGCGACG	20	7.028205E-4	45.0	32
GGCGAAC	20	7.028205E-4	45.0	32
CGATCGA	25	3.886594E-5	45.0	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content