##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933833.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421899
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.082723590243162	28.0	16.0	31.0	16.0	34.0
2	25.66731137073091	30.0	16.0	33.0	16.0	34.0
3	26.65702454852939	30.0	16.0	33.0	16.0	34.0
4	31.008466481314247	33.0	28.0	35.0	19.0	37.0
5	33.1450939679876	35.0	32.0	35.0	28.0	37.0
6	31.37050099668404	35.0	28.0	37.0	17.0	37.0
7	33.005702786685916	35.0	32.0	35.0	28.0	37.0
8	31.932877300017303	35.0	30.0	37.0	17.0	37.0
9	34.06301745204421	35.0	32.0	38.0	28.0	39.0
10	33.292266632535274	34.0	32.0	37.0	27.0	39.0
11	33.911360301873195	35.0	32.0	37.0	27.0	39.0
12	34.05002619110261	35.0	32.0	39.0	27.0	39.0
13	34.13012593061372	35.0	32.0	37.0	27.0	39.0
14	34.13594959931169	36.0	32.0	38.0	25.0	40.0
15	34.68926212197706	36.0	32.0	39.0	27.0	40.0
16	34.604509610119955	36.0	32.0	39.0	27.0	40.0
17	33.95571451935179	36.0	32.0	38.0	26.0	40.0
18	34.309863261112255	36.0	32.0	39.0	27.0	40.0
19	34.08421446839172	36.0	32.0	39.0	27.0	40.0
20	33.523812571255206	35.0	31.0	39.0	25.0	40.0
21	33.66497668873356	36.0	32.0	39.0	25.0	40.0
22	34.72964619494239	37.0	33.0	39.0	27.0	40.0
23	35.0913062130984	37.0	33.0	39.0	27.0	40.0
24	35.39277884043337	37.0	34.0	39.0	29.0	40.0
25	33.706434478394115	36.0	32.0	39.0	24.0	40.0
26	34.525542843192326	36.0	33.0	39.0	27.0	40.0
27	34.7207696628814	37.0	33.0	39.0	27.0	40.0
28	34.770447429361056	37.0	33.0	39.0	27.0	40.0
29	35.226393046677046	37.0	34.0	40.0	27.0	40.0
30	33.70490093600601	36.0	32.0	39.0	24.0	40.0
31	34.13654452842979	36.0	32.0	39.0	25.0	40.0
32	34.46836802173032	37.0	33.0	39.0	26.0	40.0
33	34.81048307770343	37.0	33.0	39.0	26.0	40.0
34	35.10696635924712	38.0	34.0	40.0	26.0	40.0
35	35.0912754000365	38.0	34.0	40.0	26.0	41.0
36	34.985804659409006	38.0	34.0	40.0	26.0	41.0
37	35.42727761857696	38.0	35.0	40.0	29.0	41.0
38	34.91791400311449	37.0	34.0	40.0	27.0	41.0
39	35.00624794085788	37.0	34.0	40.0	27.0	40.0
40	34.364468747259416	37.0	33.0	40.0	25.0	40.0
41	33.878335810229466	36.0	32.0	39.0	24.0	40.0
42	34.76850620646174	37.0	33.0	40.0	27.0	40.0
43	34.9202297232276	37.0	34.0	40.0	27.0	40.0
44	35.708676721205784	38.0	35.0	40.0	30.0	41.0
45	35.88828368875015	38.0	35.0	40.0	30.0	41.0
46	35.616398711539965	38.0	35.0	40.0	30.0	41.0
47	35.04136535047488	37.0	34.0	40.0	27.0	41.0
48	35.30879191465256	38.0	35.0	40.0	29.0	41.0
49	35.670086916536896	38.0	35.0	40.0	30.0	41.0
50	35.25812575995677	38.0	34.0	40.0	28.0	41.0
51	35.049526071405715	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	2.0
11	1.0
12	13.0
13	13.0
14	2.0
15	33.0
16	83.0
17	290.0
18	715.0
19	1462.0
20	2184.0
21	2736.0
22	3153.0
23	3309.0
24	3504.0
25	4452.0
26	5882.0
27	7534.0
28	9969.0
29	13043.0
30	17862.0
31	24620.0
32	32226.0
33	41226.0
34	47630.0
35	54687.0
36	57069.0
37	52727.0
38	31837.0
39	3630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.360145437652136	3.4911199125857135	37.211749731570826	13.93698491819132
2	15.184439877790656	34.86071310906165	33.630086821727474	16.32476019142022
3	13.50323181614557	34.79529460842524	36.15296552018374	15.548508055245449
4	14.795721250820696	2.9452546699565536	63.762417071384384	18.49660700783837
5	38.19753068862453	3.8144200389192675	35.742440726334976	22.24560854612123
6	16.379275608617228	28.99035077115613	39.820430956224115	14.80994266400252
7	56.724002664144734	1.4380218962358289	36.912863031199414	4.925112408420024
8	52.490287959914575	24.100791895690673	14.287068706017317	9.121851438377432
9	49.094925562753176	3.5318879637069536	13.989841170517114	33.38334530302276
10	42.84105911604436	17.243700506519332	22.29870182200005	17.616538555436254
11	35.42269595329688	16.981078409761576	28.199403174693472	19.39682246224807
12	23.702592326599493	14.152202304342982	30.751672793725515	31.39353257533201
13	26.147490276108737	18.233984911080615	41.912637858824034	13.705886953986617
14	19.846219118793833	29.789357168421827	26.84173226293497	23.522691449849372
15	11.142951275068205	20.430719200566962	46.03139613983441	22.39493338453042
16	14.888871507161666	24.025181382273956	24.885339856221513	36.20060725434286
17	16.989137210564614	27.379538704761092	34.239711400121834	21.391612684552463
18	15.239903389199785	22.825131133280713	33.23473153527266	28.70023394224684
19	15.684085527578876	26.54000127992719	28.072595573822174	29.703317618671765
20	17.682431103178722	26.600442285949953	41.25442345205843	14.462703158812893
21	18.57766906297479	34.25132555422032	29.558496227770153	17.612509155034736
22	14.935328123555639	19.428109571248093	37.94960405215466	27.686958253041606
23	17.489730954564955	31.583862488415477	31.055774012263598	19.870632544755974
24	20.503722454900345	24.652345703592566	25.367919810191538	29.476012031315552
25	14.403684294108304	37.848632018563684	22.29253920962126	25.45514447770675
26	15.519117134669672	20.546860741551885	32.27597126326443	31.65805086051401
27	21.516050049893458	26.001246743888938	27.127345644336675	25.355357561880925
28	13.216433316978707	25.472210173524946	34.1406355549551	27.170720954541256
29	19.85024851919535	17.91945465620919	28.17546379583739	34.05483302875807
30	18.016871336504707	27.94815820848118	35.36960267741806	18.66536777759606
31	26.25438789852548	18.271671656012458	22.71799648731094	32.75594395815112
32	23.59403553931154	28.376696792360256	22.917570318962596	25.11169734936561
33	18.919694049997748	17.029905261685855	29.989168023626505	34.06123266468989
34	22.376682570947075	20.9009739297794	23.824422432857155	32.897921066416366
35	15.095556045404232	25.520800001896188	31.410124224044147	27.97351972865544
36	22.1185639216969	20.049585327293972	28.997224454194015	28.834626296815113
37	15.011412684078419	19.679117513907357	37.53813116409378	27.77133863792045
38	26.381195499396775	20.71633258196867	27.464393136745997	25.438078781888557
39	19.013792400550845	19.49281700122541	26.78745386929099	34.70593672893275
40	16.541873765996126	23.958340740319365	35.467967451925695	24.03181804175881
41	18.296322105527626	22.520792891189597	27.97494186997362	31.207943133309158
42	17.674135278822657	23.68979305473585	34.79292437289494	23.84314729354656
43	20.469354039710925	17.513196286314972	32.28900755868111	29.728442115292996
44	20.500167101604884	16.21549233347318	35.00197914666781	28.282361418254133
45	20.075894941680357	16.41767342420816	28.229031118822277	35.2774005152892
46	27.932988701087226	22.26694066589397	26.43571091659378	23.36435971642502
47	12.623637410849517	18.45038741499743	47.55687972713849	21.36909544701457
48	20.3695671238851	23.99104999063757	25.77204496810848	29.867337917368847
49	18.969231972581117	17.61345724924686	39.39141832523898	24.025892452933046
50	22.13942199436358	16.38354203257178	32.88393667678757	28.593099296277067
51	19.191323041770662	16.78268969587508	27.805707053109867	36.22028020924439
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	74.0
1	195.0
2	316.0
3	1454.0
4	2592.0
5	1796.0
6	1000.0
7	1054.5
8	1109.0
9	1217.0
10	1325.0
11	1398.5
12	1472.0
13	1410.5
14	1349.0
15	1265.0
16	1181.0
17	1136.5
18	1092.0
19	1135.0
20	1178.0
21	1251.0
22	1324.0
23	1527.0
24	1730.0
25	1939.0
26	2550.0
27	2952.0
28	3219.5
29	3487.0
30	3866.0
31	4245.0
32	4844.5
33	5444.0
34	6151.0
35	6858.0
36	7153.5
37	7449.0
38	8197.0
39	8945.0
40	10158.5
41	11372.0
42	12636.0
43	13900.0
44	16949.5
45	19999.0
46	71388.0
47	122777.0
48	89133.0
49	55489.0
50	49727.5
51	43966.0
52	34469.5
53	24973.0
54	22428.5
55	19884.0
56	17928.0
57	15972.0
58	13965.5
59	11959.0
60	9966.0
61	7973.0
62	6868.0
63	5763.0
64	4846.5
65	3930.0
66	3354.0
67	2778.0
68	2191.0
69	1604.0
70	1234.0
71	864.0
72	773.0
73	682.0
74	556.5
75	312.5
76	194.0
77	145.0
78	96.0
79	53.5
80	11.0
81	10.0
82	9.0
83	5.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.932602580836797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.08148307435192	22.473841940444007
2	9.907229762786319	5.930983423330417
3	3.739986470363358	3.3584258602527886
4	1.9525364162788512	2.3377798629234467
5	1.3277910354249067	1.987212068688576
6	1.0501270821436683	1.885982196550811
7	0.8475524682306912	1.775861583856961
8	0.7869650159258936	1.884472885338534
9	0.6380050058418281	1.7187435256003107
>10	4.500260872648493	21.617093292085972
>50	0.08761567564193921	1.7821522601219524
>100	0.06372049137595578	3.8504025419357233
>500	0.0031860245687977892	0.754106702176018
>1k	0.007965061421994473	5.427517886511872
>5k	0.0031860245687977892	6.548068471471495
>10k+	0.002389518426598342	16.66735549871111
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	25964	6.154079530882984	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	24073	5.705867992102375	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	19872	4.7101320458213936	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	9284	2.2005266663348335	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	6149	1.457457827584327	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTA	6148	1.4572208040312966	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	5884	1.394646586031254	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	4989	1.182510506068988	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	4195	0.9943138049627992	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	2450	0.5807077049246384	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGT	2205	0.5226369344321745	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	1931	0.45769248090182724	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATG	1866	0.442285949954847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1592	0.3773414964244997	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTAT	1253	0.2969905119471722	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1180	0.2796877925759483	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	1104	0.26167400254563294	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCTCAT	926	0.21948381010621024	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	869	0.20597346758347376	No Hit
TGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCTT	832	0.19720359612134655	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTGCTCATTCG	536	0.12704462442432904	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	478	0.11329725834856211	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	460	0.10903083439401373	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	452	0.10713464596977001	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3702355303046463E-4	0.0	0.0	0.5148151571821692	0.0
2	2.3702355303046463E-4	0.0	0.0	2.9319813509868475	0.0
3	2.3702355303046463E-4	0.0	0.0	3.6674654360403793	0.0
4	2.3702355303046463E-4	0.0	0.0	6.129192057814785	0.0
5	2.3702355303046463E-4	0.0	0.0	12.443736534099394	0.0
6	2.3702355303046463E-4	0.0	0.0	13.388038369372765	0.0
7	2.3702355303046463E-4	0.0	0.0	15.153626815896695	0.0
8	2.3702355303046463E-4	0.0	0.0	17.36932298962548	0.0
9	2.3702355303046463E-4	0.0	0.0	17.852139967148535	0.0
10	2.3702355303046463E-4	0.0	0.0	24.73151157030474	0.0
11	2.3702355303046463E-4	0.0	0.0	26.498759181699885	0.0
12	2.3702355303046463E-4	0.0	0.0	34.65995415964484	0.0
13	2.3702355303046463E-4	0.0	0.0	35.62345490271368	0.0
14	2.3702355303046463E-4	0.0	0.0	36.099161173645825	0.0
15	2.3702355303046463E-4	0.0	0.0	38.031377177950176	0.0
16	2.3702355303046463E-4	0.0	0.0	39.06740712824634	0.0
17	2.3702355303046463E-4	0.0	0.0	39.90670752952721	0.0
18	2.3702355303046463E-4	0.0	0.0	40.63342174311861	0.0
19	2.3702355303046463E-4	0.0	0.0	43.70903936724192	0.0
20	2.3702355303046463E-4	0.0	0.0	44.55213214537129	0.0
21	2.3702355303046463E-4	0.0	0.0	45.31274072704605	0.0
22	2.3702355303046463E-4	0.0	0.0	46.195416438531495	0.0
23	2.3702355303046463E-4	0.0	0.0	46.87282975309257	0.0
24	2.3702355303046463E-4	0.0	0.0	47.38669681606261	0.0
25	2.3702355303046463E-4	0.0	0.0	47.84438929696444	0.0
26	2.3702355303046463E-4	0.0	0.0	48.338109357926896	0.0
27	2.3702355303046463E-4	0.0	0.0	48.88444864766212	0.0
28	2.3702355303046463E-4	0.0	0.0	49.32957888025333	0.0
29	2.3702355303046463E-4	0.0	0.0	49.83799440150368	0.0
30	2.3702355303046463E-4	0.0	0.0	50.35328360579191	0.0
31	2.3702355303046463E-4	0.0	0.0	50.81524251064828	0.0
32	4.7404710606092925E-4	0.0	0.0	51.26961666180768	0.0
33	4.7404710606092925E-4	0.0	0.0	51.67943038499736	0.0
34	4.7404710606092925E-4	0.0	0.0	52.18429055295225	0.0
35	4.7404710606092925E-4	0.0	0.0	52.623258173164665	0.0
36	4.7404710606092925E-4	0.0	0.0	53.06530709956648	0.0
37	4.7404710606092925E-4	0.0	0.0	53.506170908203146	0.0
38	4.7404710606092925E-4	0.0	0.0	54.020037971173196	0.0
39	4.7404710606092925E-4	0.0	0.0	55.0003673865072	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	115	0.0	45.000004	1
ATCCGTC	35	1.2094824E-7	45.000004	6
GCGATAC	35	1.2094824E-7	45.000004	33
GTACGGG	30	2.1622218E-6	45.000004	3
CGGGTAT	20	7.028205E-4	45.0	6
CGCGGGC	90	0.0	45.0	4
CCGATCG	25	3.886594E-5	45.0	40
CCGATCA	20	7.028205E-4	45.0	40
GCGAACA	20	7.028205E-4	45.0	33
GGGTACG	40	6.7975634E-9	45.0	7
CTCCCGG	20	7.028205E-4	45.0	2
CGATTGT	40	6.7975634E-9	45.0	10
CCTCGAA	25	3.886594E-5	45.0	42
TGTTGCG	20	7.028205E-4	45.0	1
TTGCGCG	20	7.028205E-4	45.0	1
GAGCATT	20	7.028205E-4	45.0	34
TCCCCCG	20	7.028205E-4	45.0	1
GGCGACG	20	7.028205E-4	45.0	32
GGCGAAC	20	7.028205E-4	45.0	32
CGATCGA	25	3.886594E-5	45.0	41
>>END_MODULE