FastQCFastQC Report
Sat 14 Jan 2017
SRR2933831.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933831.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1081575
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC503444.654693386958833No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG472144.365300603286873No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC395133.653283406143818No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT172331.5933245498462891No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC127651.1802232854864434Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA110191.0187920393870051No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT106070.9806994429420058No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC90330.8351709312807711No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT78280.7237593324549847No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.6892725885860898No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC40090.37066315327184896Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT39670.3667799274206597Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT35040.3239719852992164No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT32970.30483322931835516No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG31890.29484779141529716No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG26440.2444583131081987No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC23930.22125141575942495Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT23930.22125141575942495No Hit
TGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCTT20760.19194230635878234Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG20370.18833645378267802No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC14430.1334165453158588No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC11320.10466218246538612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG207.0331007E-445.0000047
ACGACCG207.0331007E-445.00000436
TATTGCG253.890653E-545.01
TGATACC53000.043.811324
CGTTTTT47300.043.6680761
TACGGCT57400.043.6672487
GATACCT53550.043.571435
ACGGCTG57850.043.3275688
CGGCTGT60200.042.5332269
GCGACTG11800.042.52118711
CCGATGA15800.041.8670918
GGCGACT12050.041.82572610
ACCACCT21900.041.71232614
CGATGAA15850.041.02523419
ATGATAC193400.040.9048583
AATGATA192950.040.7320562
CATACGA2100.040.71428318
GAATCTG49200.040.6097561
GCGATAC501.0822987E-940.533
TCAAGCG501.0822987E-940.517
Produced by FastQC (version 0.11.5)