##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933831.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1081575
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.884756951667708	30.0	16.0	33.0	16.0	34.0
2	26.782706700876037	31.0	16.0	33.0	16.0	34.0
3	27.79921041074359	31.0	25.0	33.0	16.0	34.0
4	31.718572452210896	35.0	28.0	35.0	19.0	37.0
5	33.448643875829234	35.0	33.0	37.0	28.0	37.0
6	32.2179118415274	35.0	32.0	37.0	17.0	37.0
7	33.23716986801655	35.0	32.0	35.0	28.0	37.0
8	32.60298083812958	35.0	32.0	37.0	25.0	37.0
9	34.4082167209856	35.0	32.0	39.0	28.0	39.0
10	33.42840672167903	35.0	31.0	37.0	27.0	39.0
11	34.0413998104616	35.0	32.0	39.0	27.0	39.0
12	34.185961213970366	35.0	32.0	39.0	27.0	39.0
13	34.43479925109216	35.0	33.0	39.0	27.0	39.0
14	34.51511545662576	37.0	32.0	39.0	25.0	40.0
15	34.98208630931743	37.0	33.0	39.0	27.0	40.0
16	34.85082310519381	36.0	32.0	39.0	27.0	40.0
17	34.23346601021658	36.0	32.0	38.0	27.0	40.0
18	34.58547118785105	36.0	32.0	39.0	27.0	40.0
19	34.300824260915796	36.0	32.0	39.0	27.0	40.0
20	34.08172341261586	36.0	32.0	39.0	25.0	40.0
21	33.90511522548136	36.0	32.0	39.0	25.0	40.0
22	34.829324827219565	37.0	33.0	39.0	27.0	40.0
23	35.428267110463906	37.0	34.0	39.0	29.0	40.0
24	35.72742250884127	37.0	34.0	39.0	30.0	40.0
25	33.86721124286342	36.0	32.0	39.0	24.0	40.0
26	34.486582067817764	36.0	32.0	39.0	27.0	40.0
27	34.944081547742876	37.0	33.0	39.0	27.0	40.0
28	35.1184198969096	37.0	34.0	39.0	27.0	40.0
29	35.606183574879225	38.0	34.0	40.0	29.0	40.0
30	33.81452234010587	36.0	32.0	39.0	24.0	40.0
31	34.64900261193167	37.0	33.0	39.0	26.0	40.0
32	34.79950627557035	37.0	33.0	39.0	27.0	40.0
33	35.220405427270414	38.0	34.0	39.0	27.0	40.0
34	35.16625199362041	38.0	34.0	40.0	27.0	41.0
35	35.34374500150244	38.0	34.0	40.0	27.0	41.0
36	35.03294085014909	38.0	34.0	40.0	25.0	41.0
37	35.23582738136514	38.0	34.0	40.0	27.0	41.0
38	35.16015347987888	38.0	34.0	40.0	28.0	41.0
39	34.83821556526362	37.0	34.0	40.0	26.0	41.0
40	34.68451748607355	37.0	33.0	40.0	25.0	41.0
41	34.018249312345425	37.0	33.0	39.0	24.0	40.0
42	34.77208977648337	37.0	33.0	40.0	26.0	41.0
43	34.88859672237247	38.0	34.0	40.0	26.0	40.0
44	35.45236899891362	38.0	35.0	40.0	29.0	41.0
45	35.7895596699258	38.0	35.0	40.0	30.0	41.0
46	35.51264036243441	38.0	35.0	40.0	29.0	41.0
47	35.023217992279776	38.0	34.0	40.0	26.0	41.0
48	35.26158888657744	38.0	34.0	40.0	28.0	41.0
49	35.59601691976978	38.0	35.0	40.0	29.0	41.0
50	35.25717495319326	38.0	34.0	40.0	27.0	41.0
51	34.57257980260269	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	12.0
11	13.0
12	24.0
13	24.0
14	54.0
15	91.0
16	258.0
17	913.0
18	1986.0
19	3369.0
20	4724.0
21	6077.0
22	7185.0
23	7833.0
24	9190.0
25	12248.0
26	15808.0
27	19403.0
28	23969.0
29	31193.0
30	41451.0
31	56159.0
32	74963.0
33	95182.0
34	112086.0
35	137430.0
36	156542.0
37	155601.0
38	96891.0
39	10890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35910593347664	3.1983912350045074	41.488662367380904	14.953840464137947
2	18.476665973233477	26.804613642142243	38.13521947160391	16.583500913020362
3	14.953470633104502	26.794535746480825	42.06541386404087	16.186579756373806
4	15.007003675195897	3.0495342440422535	62.59029655825995	19.353165522501907
5	32.49575850033516	3.8631625176247604	41.68384069528234	21.95723828675774
6	16.530337701962416	22.737766682846775	45.49504195270785	15.236853662482952
7	63.35168619836812	1.2872893696692322	31.002658160552897	4.35836627140975
8	60.847097982109425	18.30682107112313	13.850542033608395	6.995538913159051
9	57.36365947807596	3.2311212814645307	13.540531169821787	25.864688070637726
10	41.425606176178256	18.685712964889166	22.72112428634168	17.167556572590897
11	32.48734484432425	19.353165522501907	28.945288121489494	19.214201511684347
12	23.674363775050274	16.099114716963687	31.84004807803435	28.386473429951693
13	25.209994683678893	18.18727318956152	40.8298083812958	15.77292374546379
14	20.0626863601692	27.137646487760904	29.64731988072949	23.152347271340407
15	13.844439821556525	21.25132330166655	43.17083882301274	21.733398053764187
16	17.222846312091164	23.83403832374084	26.822550447264405	32.12056491690359
17	18.022790837436144	26.85602015579132	34.15371102327624	20.96747798349629
18	17.252062963733444	23.280216351154568	33.37660356424659	26.091117120865405
19	16.929108013776208	26.84520259806301	29.256131105101357	26.969558283059424
20	19.255437671913644	26.28019323671498	38.86517347386913	15.599195617502254
21	20.510274368397937	31.943323394124313	30.54221852391189	17.004183713565865
22	16.826017613202968	19.767561195478816	37.11272912188244	26.293692069435775
23	18.585303839308416	29.164875297598407	32.004253056884636	20.245567806208538
24	20.64507778008922	23.694519566373113	28.268035041490418	27.39236761204725
25	16.208399787347155	34.816263319695814	25.435961445114764	23.539375447842268
26	16.863000716547628	21.40951852622333	34.57235975313778	27.155121004091253
27	21.376973395280032	25.353766497931257	29.708989205556712	23.560270901232
28	15.211150405658413	24.777015001271295	36.31047315257842	23.701361440491876
29	20.07637010840672	19.4729907773386	35.788826479902	24.661812634352682
30	18.40889443635439	26.63587823313224	35.52809560132214	19.427131729191228
31	25.566049511129602	20.977555879157713	28.21995700714236	25.236437602570327
32	25.114300903774588	26.222499595497307	30.185886323186097	18.47731317754201
33	21.47784480965259	19.859926496082103	25.473314379492866	33.18891431477244
34	23.802047939347712	24.54226475279107	31.642188475140422	20.0134988327208
35	22.921110417677923	20.9679402722881	31.931211427778933	24.179737882255044
36	27.63053879758685	22.44412084229018	29.576589695582832	20.348750664540137
37	18.805075930934052	21.450939601969353	41.16644707949056	18.57753738760604
38	27.532348658206786	20.534590758847052	24.27829785266856	27.654762730277604
39	21.681621709081664	26.115895800106326	32.18602500982364	20.01645748098837
40	23.588562975290664	20.300302799158633	29.376973395280032	26.73416083027067
41	25.329265191965423	28.266278344081545	24.041883364537828	22.362573099415204
42	25.518803596606798	19.06395765434667	34.48850056630377	20.92873818274276
43	27.588193144257218	19.078288606892727	28.29854610174976	25.034972147100294
44	21.965282111735203	19.494348519520145	33.49485703719113	25.04551233155352
45	21.906109146383745	17.213877909530083	29.41293946328271	31.46707348080346
46	27.9551579871946	22.725839632018122	29.051799459122112	20.267202921665163
47	15.71060721632804	19.805468876407094	42.21445577051984	22.269468136745026
48	21.39047222800083	25.99597808751127	27.54177934955967	25.071770334928228
49	22.32975059519682	17.429119570996	37.55939255252756	22.681737281279617
50	24.823243880452118	18.118946906132262	30.53260291704228	26.525206296373344
51	20.54115525969073	18.44476804659871	28.64831380163188	32.36576289207868
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	113.0
1	393.5
2	674.0
3	6506.0
4	12338.0
5	8416.0
6	4494.0
7	4366.5
8	4239.0
9	4311.0
10	4383.0
11	4410.0
12	4437.0
13	4225.0
14	4013.0
15	3860.5
16	3708.0
17	3530.0
18	3352.0
19	3518.0
20	3684.0
21	3703.0
22	3722.0
23	4129.0
24	4536.0
25	5137.5
26	6705.5
27	7672.0
28	8941.5
29	10211.0
30	11854.0
31	13497.0
32	15598.5
33	17700.0
34	19771.0
35	21842.0
36	23950.0
37	26058.0
38	28635.5
39	31213.0
40	34015.5
41	36818.0
42	40391.0
43	43964.0
44	51188.0
45	58412.0
46	159996.5
47	261581.0
48	195541.5
49	129502.0
50	117337.5
51	105173.0
52	84223.5
53	63274.0
54	56192.5
55	49111.0
56	44183.5
57	39256.0
58	35866.0
59	32476.0
60	27862.0
61	23248.0
62	20209.5
63	17171.0
64	14719.0
65	12267.0
66	10316.5
67	8366.0
68	6798.5
69	5231.0
70	4328.5
71	3426.0
72	2757.0
73	2088.0
74	1664.0
75	933.5
76	627.0
77	498.0
78	369.0
79	284.0
80	199.0
81	153.0
82	107.0
83	59.5
84	12.0
85	12.5
86	13.0
87	12.0
88	11.0
89	8.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1081575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.861705273949475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.06491014088246	24.996226587391302
2	9.546746121051292	6.27444714710416
3	3.3891198581789213	3.341167737526955
4	1.8043067267994877	2.3717038351955697
5	1.249824847899725	2.053568789786975
6	0.9505864767013582	1.8742735580857244
7	0.7782253660821582	1.7901668831832325
8	0.7207058807010673	1.8946899392640535
9	0.6540719694247058	1.934452825846774
>10	4.712921770944798	24.583078708981006
>50	0.07156061817469482	1.636752625452166
>100	0.04447577406462481	2.791372699942481
>500	0.005987123431574361	1.3999960480121036
>1k	0.003706314505260319	3.1012962043295627
>5k	8.553033473677659E-4	2.278143873617233
>10k+	0.0019957078105247873	17.678662536280793
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	50344	4.654693386958833	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	47214	4.365300603286873	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	39513	3.653283406143818	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTCAGGT	17233	1.5933245498462891	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	12765	1.1802232854864434	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTA	11019	1.0187920393870051	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCT	10607	0.9806994429420058	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTC	9033	0.8351709312807711	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	7828	0.7237593324549847	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7455	0.6892725885860898	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	4009	0.37066315327184896	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	3967	0.3667799274206597	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGT	3504	0.3239719852992164	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	3297	0.30483322931835516	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATG	3189	0.29484779141529716	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG	2644	0.2444583131081987	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2393	0.22125141575942495	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTAT	2393	0.22125141575942495	No Hit
TGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCTT	2076	0.19194230635878234	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTTGTCAGG	2037	0.18833645378267802	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	1443	0.1334165453158588	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	1132	0.10466218246538612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4269699281140929	0.0
2	0.0	0.0	0.0	2.3100570926657884	0.0
3	0.0	0.0	0.0	2.909090909090909	0.0
4	0.0	0.0	0.0	4.565379192381481	0.0
5	0.0	0.0	0.0	9.47054064674202	0.0
6	0.0	0.0	0.0	10.498763377481913	0.0
7	0.0	0.0	0.0	11.950257726001434	0.0
8	0.0	0.0	0.0	13.691976978018168	0.0
9	0.0	0.0	0.0	14.164898412038	0.0
10	0.0	0.0	0.0	19.605945033862653	0.0
11	0.0	0.0	0.0	21.167094283799088	0.0
12	0.0	0.0	0.0	27.444883618796663	0.0
13	0.0	0.0	0.0	28.201188082194946	0.0
14	0.0	0.0	0.0	28.607077641402583	0.0
15	0.0	0.0	0.0	29.97425051429628	0.0
16	0.0	0.0	0.0	30.784735224094494	0.0
17	0.0	0.0	0.0	31.57423202274461	0.0
18	0.0	0.0	0.0	32.30880891292791	0.0
19	0.0	0.0	0.0	34.673970829577236	0.0
20	0.0	0.0	0.0	35.461433557543394	0.0
21	9.245775836164852E-5	0.0	0.0	36.28486235351224	0.0
22	9.245775836164852E-5	0.0	0.0	37.2251577560502	0.0
23	9.245775836164852E-5	0.0	0.0	38.10822180616231	0.0
24	9.245775836164852E-5	0.0	0.0	38.78464276633613	0.0
25	9.245775836164852E-5	0.0	0.0	39.3717495319326	0.0
26	9.245775836164852E-5	0.0	0.0	39.90865173473869	0.0
27	9.245775836164852E-5	0.0	0.0	40.5091648752976	0.0
28	9.245775836164852E-5	0.0	0.0	41.05799412893234	0.0
29	9.245775836164852E-5	0.0	0.0	41.64214224626124	0.0
30	9.245775836164852E-5	0.0	0.0	42.298684788387305	0.0
31	9.245775836164852E-5	0.0	0.0	42.864618727318955	0.0
32	9.245775836164852E-5	0.0	0.0	43.37248919399949	0.0
33	9.245775836164852E-5	0.0	0.0	43.894783070984445	0.0
34	9.245775836164852E-5	0.0	0.0	44.42992857638166	0.0
35	9.245775836164852E-5	0.0	0.0	45.00399879804914	0.0
36	9.245775836164852E-5	0.0	0.0	45.53137785174398	0.0
37	9.245775836164852E-5	0.0	0.0	46.063564708873635	0.0
38	9.245775836164852E-5	0.0	0.0	46.649561981369764	0.0
39	9.245775836164852E-5	0.0	0.0	47.53835841250029	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACG	20	7.0331007E-4	45.000004	7
ACGACCG	20	7.0331007E-4	45.000004	36
TATTGCG	25	3.890653E-5	45.0	1
TGATACC	5300	0.0	43.81132	4
CGTTTTT	4730	0.0	43.668076	1
TACGGCT	5740	0.0	43.667248	7
GATACCT	5355	0.0	43.57143	5
ACGGCTG	5785	0.0	43.327568	8
CGGCTGT	6020	0.0	42.533226	9
GCGACTG	1180	0.0	42.521187	11
CCGATGA	1580	0.0	41.86709	18
GGCGACT	1205	0.0	41.825726	10
ACCACCT	2190	0.0	41.712326	14
CGATGAA	1585	0.0	41.025234	19
ATGATAC	19340	0.0	40.904858	3
AATGATA	19295	0.0	40.732056	2
CATACGA	210	0.0	40.714283	18
GAATCTG	4920	0.0	40.609756	1
GCGATAC	50	1.0822987E-9	40.5	33
TCAAGCG	50	1.0822987E-9	40.5	17
>>END_MODULE