SRR2933830.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933830.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	478844
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	32482	6.78342007000192	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	27819	5.809616493054105	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTC	23040	4.811587907544002	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	12024	2.511047439249526	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGACCTAT	11232	2.345649104927701	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTA	6878	1.4363759387190818	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	6837	1.4278136512099975	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTC	5502	1.1490172164629817	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCT	5224	1.0909607304257753	No Hit
CTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCT	4013	0.8380599944867222	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	3832	0.8002606276783253	TruSeq Adapter, Index 16 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGC	2966	0.6194084085840065	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGACCTATCGT	2433	0.5080986709659095	No Hit
TGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTGCTT	2130	0.4448212779109689	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATG	2002	0.41809023398016887	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTAT	1616	0.33747942962635014	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTGACCTA	1310	0.27357552772928134	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTT	1222	0.25519793502685634	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	1044	0.2180250770605876	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	974	0.20340653741093132	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	847	0.17688432976084068	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	749	0.15641837425132193	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	670	0.1399203080752813	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTGACCTATCG	643	0.13428172849612818	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGC	641	0.13386405593470943	No Hit
TGCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCTG	600	0.12530176842562504	No Hit
GCACTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCGTCTTCT	482	0.10065908730191879	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTGACCTATCGTATGCCG	479	0.10003257845979066	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	35	1.2099372E-7	45.000004	1
CGGGTGC	25	3.887384E-5	45.000004	6
TCGTTAG	25	3.887384E-5	45.000004	1
ACCGGTC	35	1.2099372E-7	45.000004	17
CCCTACG	25	3.887384E-5	45.000004	32
GACCGGG	35	1.2099372E-7	45.000004	3
GGCCGGT	25	3.887384E-5	45.000004	9
TCGGGTA	25	3.887384E-5	45.000004	5
TACGGGA	25	3.887384E-5	45.000004	4
ACGGCCC	25	3.887384E-5	45.000004	14
TACGAGA	25	3.887384E-5	45.000004	35
CGTTTAC	25	3.887384E-5	45.000004	20
CGAACAC	20	7.02916E-4	45.0	34
GTTCTCG	20	7.02916E-4	45.0	1
CTCGTTA	40	6.8012014E-9	45.0	26
CTCGTCC	20	7.02916E-4	45.0	37
CTACTCA	20	7.02916E-4	45.0	29
ACGTTGT	20	7.02916E-4	45.0	13
GCGAACA	20	7.02916E-4	45.0	33
TTTCGGT	20	7.02916E-4	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content