SRR2933827.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727103
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	40462	5.564823690728824	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	33830	4.652710826389109	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	29236	4.020888374824475	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	12169	1.6736280829538592	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	11849	1.6296178120568887	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	9024	1.2410896392945703	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	8536	1.1739739761766903	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	8148	1.1206115227141136	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	6364	0.8752542624635025	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	5414	0.744598770738121	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	4875	0.6704689706960362	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGT	2929	0.4028315108038338	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2919	0.40145618983830356	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATG	2748	0.37793820132773487	No Hit
TGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCTT	2269	0.312060327078832	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTAT	1777	0.24439453557473975	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.24013104058159573	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTT	1698	0.23352949994705016	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	1521	0.2091863188571633	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTAGGGA	1461	0.20093439306398134	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	990	0.13615677558750275	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	767	0.10548711805617636	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	35	1.2111013E-7	45.000004	2
CACCGTT	25	3.8893857E-5	45.000004	16
ATCGGAA	25	3.8893857E-5	45.000004	23
CGGGTAT	40	6.8084773E-9	45.0	6
TCGTTCG	20	7.031575E-4	45.0	1
ACGTTTA	20	7.031575E-4	45.0	23
GCAACTA	20	7.031575E-4	45.0	9
TTCACGC	20	7.031575E-4	45.0	43
TTTCACG	20	7.031575E-4	45.0	1
TGCGCCG	20	7.031575E-4	45.0	1
CTACGAT	20	7.031575E-4	45.0	14
CACACCG	20	7.031575E-4	45.0	17
GCATATC	75	0.0	45.0	28
CCGATCG	55	1.8189894E-12	44.999996	40
CGATCGA	60	0.0	44.999996	41
TACGGCT	4530	0.0	43.956955	7
GATACCT	3705	0.0	43.42105	5
ACGGCTG	4620	0.0	43.149353	8
TGATACC	3735	0.0	43.012047	4
CGGCTGT	4785	0.0	42.695927	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content