##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933827.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	727103
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.43849220811907	30.0	16.0	31.0	16.0	34.0
2	26.422930451394095	30.0	16.0	33.0	16.0	34.0
3	27.417591455405905	31.0	25.0	33.0	16.0	34.0
4	31.47985636147836	35.0	28.0	35.0	19.0	37.0
5	33.3428358843245	35.0	32.0	35.0	28.0	37.0
6	31.90055741758733	35.0	31.0	37.0	17.0	37.0
7	33.1653630916115	35.0	32.0	35.0	28.0	37.0
8	32.2790127395981	35.0	32.0	37.0	17.0	37.0
9	34.25775715407583	35.0	32.0	39.0	28.0	39.0
10	33.42295658249244	35.0	32.0	37.0	27.0	39.0
11	33.9941108756256	35.0	32.0	39.0	27.0	39.0
12	34.17104866848301	35.0	32.0	39.0	27.0	39.0
13	34.38753381570424	35.0	33.0	38.0	27.0	39.0
14	34.41581866668134	37.0	32.0	39.0	25.0	40.0
15	34.86343888004863	37.0	32.0	39.0	27.0	40.0
16	34.50207192103457	36.0	32.0	39.0	27.0	40.0
17	34.096029035776226	36.0	32.0	38.0	27.0	40.0
18	34.41456574928174	36.0	32.0	39.0	27.0	40.0
19	34.05726148839985	36.0	32.0	39.0	26.0	40.0
20	34.16862672826271	36.0	32.0	39.0	26.0	40.0
21	34.03926541356589	36.0	32.0	39.0	25.0	40.0
22	35.10084953576041	37.0	33.0	39.0	27.0	40.0
23	35.67102597568707	37.0	34.0	39.0	30.0	40.0
24	35.938448885508656	38.0	34.0	40.0	30.0	40.0
25	34.00228028216085	36.0	32.0	39.0	24.0	40.0
26	34.77051944497547	37.0	33.0	39.0	27.0	40.0
27	35.13447613336763	37.0	33.0	39.0	28.0	40.0
28	35.33721082157548	37.0	34.0	39.0	28.0	40.0
29	35.80337861348392	38.0	34.0	40.0	30.0	40.0
30	34.059599534041254	37.0	32.0	39.0	24.0	40.0
31	34.763347146140234	37.0	33.0	39.0	26.0	40.0
32	34.718397531023804	37.0	33.0	39.0	27.0	40.0
33	35.4858197531849	38.0	34.0	39.0	29.0	40.0
34	35.63374515027444	38.0	34.0	40.0	30.0	40.0
35	35.83119998129563	38.0	35.0	40.0	30.0	41.0
36	35.773442002027224	38.0	35.0	40.0	29.0	41.0
37	36.09707978099389	38.0	35.0	40.0	30.0	41.0
38	35.729453736265704	38.0	35.0	40.0	30.0	41.0
39	35.49334000822442	38.0	34.0	40.0	29.0	41.0
40	35.26458562266969	37.0	34.0	40.0	28.0	41.0
41	34.60443843581996	37.0	33.0	40.0	26.0	40.0
42	35.5145873418209	38.0	34.0	40.0	30.0	41.0
43	35.608452997718345	38.0	35.0	40.0	30.0	40.0
44	36.139237494550294	38.0	35.0	40.0	31.0	41.0
45	36.358599813231415	38.0	35.0	40.0	31.0	41.0
46	36.12726807618728	38.0	35.0	40.0	31.0	41.0
47	35.660031659888624	38.0	35.0	40.0	30.0	41.0
48	35.87252700098885	38.0	35.0	40.0	30.0	41.0
49	36.202078660107304	38.0	35.0	40.0	31.0	41.0
50	35.90725248004753	38.0	35.0	40.0	30.0	41.0
51	35.28891642587089	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	7.0
11	14.0
12	17.0
13	20.0
14	27.0
15	46.0
16	144.0
17	350.0
18	827.0
19	1646.0
20	2496.0
21	3178.0
22	3638.0
23	4208.0
24	4591.0
25	5873.0
26	7955.0
27	10831.0
28	14503.0
29	20050.0
30	27199.0
31	37596.0
32	51675.0
33	67028.0
34	81624.0
35	99634.0
36	111961.0
37	103580.0
38	59962.0
39	6416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.27062328170837	3.5201340112748816	38.94853961543275	14.260703091583999
2	15.729133286480732	29.37135453986574	37.50954128919837	17.38997088445516
3	15.70217699555634	29.167807036967254	38.66233532250589	16.46768064497052
4	15.843697522909409	3.393466950349538	61.06356320906392	19.69927231767714
5	34.97248670408457	3.8540619417056456	38.14658996043202	23.02686139377777
6	17.650456675326602	24.5992658536686	41.06405832461151	16.686219146393288
7	60.87390644791728	1.56800343280113	32.22528307543773	5.332807043843857
8	57.00650389284599	19.8674740717615	13.427533650665724	9.698488384726787
9	53.12851136634011	4.1534693159015985	14.078197999458123	28.639821318300157
10	41.15029094915026	20.506723256539992	21.199334894781067	17.14365089952868
11	33.531287864305334	18.84918642888284	27.208249725279636	20.41127598153219
12	24.086683729815444	15.508256739416563	30.71861895769925	29.68644057306874
13	24.218164414120142	19.672866155138955	39.8099031361444	16.2990662945965
14	20.28378372802753	28.151444843440338	26.421016004610077	25.14375542392206
15	12.785121227666508	22.099619998817225	43.011512811802454	22.103745961713813
16	15.88770779380638	24.22462842265814	24.41112194558405	35.47654183795143
17	16.951105964354433	27.74778814005718	32.72645003527698	22.5746558603114
18	16.226861943906158	23.65882137743896	32.2239077544722	27.89040892418268
19	16.180926223657448	26.44948514859655	28.285951233869206	29.083637393876792
20	18.946971749532047	26.19725128351829	39.11949201144817	15.736284955501489
21	21.47549934465956	32.62577654060016	27.90044876723105	17.998275347509225
22	16.99002754767894	20.670111387245	34.48782359583168	27.852037469244383
23	18.635186486646322	30.235743766701557	29.673512555992755	21.45555719065937
24	22.212671382183817	22.48993608883473	25.436561257483465	29.860831271497982
25	15.592838978796678	35.38013183826775	22.44633841422742	26.58069076870815
26	16.39286318444567	20.912580473467994	32.59084338807569	30.103712954010643
27	21.210474994601867	26.62813934201894	24.644651445531103	27.51673421784809
28	12.726119958245254	24.95657424051338	37.40089093292147	24.916414868319894
29	20.15642900661942	20.915743711688712	30.563207688594325	28.36461959309754
30	20.50741091702276	26.315391354457347	29.414539618183394	23.7626581103365
31	25.31965897541339	21.176091970463606	27.149661052148044	26.35458800197496
32	27.337667428136044	32.12103374625053	21.849586647283807	18.691712178329617
33	25.60545067205059	20.904878676061024	21.614406762178124	31.875263889710258
34	29.903328689332874	20.92454576586811	29.84020145701503	19.331924087783985
35	23.84338945101313	23.100853661723306	22.78824320625826	30.267513681005305
36	21.56076924452244	25.494737334325396	32.59978297435164	20.34471044680052
37	19.02742802601557	25.12766416862535	30.215389016411702	25.62951878894737
38	26.70584497657141	23.07939865466103	27.152274161982554	23.06248220678501
39	27.092172635788874	29.295436822568465	25.76870127065904	17.843689270983614
40	32.904691632409715	19.07308868207118	26.294073879491624	21.72814580602748
41	26.96330506131869	26.95587832810482	22.119287088624308	23.961529521952187
42	26.462688229865645	19.357642589839404	31.846107085241016	22.33356209505393
43	23.976520520476466	25.47768335435282	25.361606264862065	25.18418986030865
44	22.987664746260158	18.17802979770404	30.741036689437394	28.093268766598406
45	20.788939118666818	15.769842787060432	29.806506093359538	33.63471200091321
46	29.46212572359074	25.140179589411677	25.662939088409757	19.73475559858782
47	17.16276786094955	18.698451251060717	42.576086194115554	21.56269469387418
48	23.845589964557977	23.84022621279241	25.705298974148093	26.608884848501518
49	23.33273277651172	16.04243140242854	38.1709331415219	22.453902679537837
50	25.544523953277597	18.59887801315632	29.147039690387743	26.70955834317834
51	21.416910671527965	16.75567285515257	26.63872931345353	35.188687159865935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	106.0
1	286.5
2	467.0
3	1821.0
4	3175.0
5	2225.5
6	1276.0
7	1400.5
8	1525.0
9	1633.0
10	1741.0
11	1851.5
12	1962.0
13	1837.5
14	1713.0
15	1620.5
16	1528.0
17	1501.0
18	1474.0
19	1457.0
20	1440.0
21	1641.5
22	1843.0
23	2081.5
24	2320.0
25	2686.5
26	3448.5
27	3844.0
28	4341.0
29	4838.0
30	5628.0
31	6418.0
32	7800.5
33	9183.0
34	10980.0
35	12777.0
36	13322.5
37	13868.0
38	15050.0
39	16232.0
40	17806.0
41	19380.0
42	21896.0
43	24412.0
44	29354.0
45	34296.0
46	113446.0
47	192596.0
48	144185.5
49	95775.0
50	88748.0
51	81721.0
52	63688.5
53	45656.0
54	39690.5
55	33725.0
56	30409.5
57	27094.0
58	24679.0
59	22264.0
60	20282.0
61	18300.0
62	15900.5
63	13501.0
64	11731.0
65	9961.0
66	8318.5
67	6676.0
68	5442.0
69	4208.0
70	3628.5
71	3049.0
72	2336.0
73	1623.0
74	1355.0
75	791.5
76	496.0
77	403.0
78	310.0
79	210.0
80	110.0
81	83.5
82	57.0
83	33.5
84	10.0
85	11.5
86	13.0
87	6.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	727103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.53491170896023
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42763733940392	19.218609620896743
2	11.369232411331623	6.033631564666673
3	4.208791100895401	3.3503970079115097
4	2.179643233728095	2.313465630560303
5	1.2687933303180672	1.6833659498453766
6	0.8826716187344607	1.4052968082674355
7	0.6481322646772392	1.2038692693257291
8	0.5698400421346921	1.2096524165019387
9	0.4871034974820341	1.1632723468930355
>10	5.769900297356251	29.392496068555285
>50	0.10201520559783371	1.8578985548857667
>100	0.06888655635729184	3.6453323129092166
>500	0.006836070478204532	1.1542273260243077
>1k	0.005258515752465025	3.3408685767649895
>5k	0.0026292578762325126	5.230580940926885
>10k+	0.0026292578762325126	17.79703560506484
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	40462	5.564823690728824	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCG	33830	4.652710826389109	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTC	29236	4.020888374824475	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGGGAT	12169	1.6736280829538592	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	11849	1.6296178120568887	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCT	9024	1.2410896392945703	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTA	8536	1.1739739761766903	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTC	8148	1.1206115227141136	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCT	6364	0.8752542624635025	No Hit
CCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	5414	0.744598770738121	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCT	4875	0.6704689706960362	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTAGGGATCGT	2929	0.4028315108038338	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGC	2919	0.40145618983830356	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATG	2748	0.37793820132773487	No Hit
TGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTGCTT	2269	0.312060327078832	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTAT	1777	0.24439453557473975	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1746	0.24013104058159573	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTT	1698	0.23352949994705016	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	1521	0.2091863188571633	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTAGGGA	1461	0.20093439306398134	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGC	990	0.13615677558750275	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTAGGGATCGTATGCCGTCTTCTG	767	0.10548711805617636	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3753209655303306E-4	0.0	0.0	0.7542260174968334	0.0
2	1.3753209655303306E-4	0.0	0.0	3.723406449980264	0.0
3	1.3753209655303306E-4	0.0	0.0	4.5349833517397125	0.0
4	1.3753209655303306E-4	0.0	0.0	6.838783501099569	0.0
5	1.3753209655303306E-4	0.0	0.0	12.558743396740214	0.0
6	1.3753209655303306E-4	0.0	0.0	13.545811253701332	0.0
7	1.3753209655303306E-4	0.0	0.0	15.224940620517314	0.0
8	1.3753209655303306E-4	0.0	0.0	17.476341041090464	0.0
9	2.750641931060661E-4	0.0	0.0	17.942987444694907	0.0
10	2.750641931060661E-4	0.0	0.0	23.78493830997809	0.0
11	2.750641931060661E-4	0.0	0.0	25.573955821939947	0.0
12	2.750641931060661E-4	0.0	0.0	33.184844513088244	0.0
13	2.750641931060661E-4	0.0	0.0	34.10465917483493	0.0
14	2.750641931060661E-4	0.0	0.0	34.54476188380463	0.0
15	2.750641931060661E-4	0.0	0.0	36.135183048343904	0.0
16	2.750641931060661E-4	0.0	0.0	37.10285887969105	0.0
17	2.750641931060661E-4	0.0	0.0	37.972749390388984	0.0
18	2.750641931060661E-4	0.0	0.0	38.736465122547976	0.0
19	2.750641931060661E-4	0.0	0.0	41.27351970766178	0.0
20	2.750641931060661E-4	0.0	0.0	42.174492472180695	0.0
21	2.750641931060661E-4	0.0	0.0	42.993221042960904	0.0
22	2.750641931060661E-4	0.0	0.0	43.95635831512179	0.0
23	2.750641931060661E-4	0.0	0.0	44.72131183614976	0.0
24	2.750641931060661E-4	0.0	0.0	45.33690550032114	0.0
25	2.750641931060661E-4	0.0	0.0	45.87190535591244	0.0
26	2.750641931060661E-4	0.0	0.0	46.42162114583491	0.0
27	2.750641931060661E-4	0.0	0.0	47.003656978447346	0.0
28	2.750641931060661E-4	0.0	0.0	47.484744252189856	0.0
29	2.750641931060661E-4	0.0	0.0	48.02208215342255	0.0
30	2.750641931060661E-4	0.0	0.0	48.651291495152684	0.0
31	2.750641931060661E-4	0.0	0.0	49.16648672884034	0.0
32	2.750641931060661E-4	0.0	0.0	49.63725909534138	0.0
33	2.750641931060661E-4	0.0	0.0	50.11078210377347	0.0
34	2.750641931060661E-4	0.0	0.0	50.62556474117147	0.0
35	2.750641931060661E-4	0.0	0.0	51.15602603757652	0.0
36	2.750641931060661E-4	0.0	0.0	51.628861385525845	0.0
37	2.750641931060661E-4	0.0	0.0	52.10293452234415	0.0
38	2.750641931060661E-4	0.0	0.0	52.63972229519064	0.0
39	2.750641931060661E-4	0.0	0.0	53.42956912569471	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	35	1.2111013E-7	45.000004	2
CACCGTT	25	3.8893857E-5	45.000004	16
ATCGGAA	25	3.8893857E-5	45.000004	23
CGGGTAT	40	6.8084773E-9	45.0	6
TCGTTCG	20	7.031575E-4	45.0	1
ACGTTTA	20	7.031575E-4	45.0	23
GCAACTA	20	7.031575E-4	45.0	9
TTCACGC	20	7.031575E-4	45.0	43
TTTCACG	20	7.031575E-4	45.0	1
TGCGCCG	20	7.031575E-4	45.0	1
CTACGAT	20	7.031575E-4	45.0	14
CACACCG	20	7.031575E-4	45.0	17
GCATATC	75	0.0	45.0	28
CCGATCG	55	1.8189894E-12	44.999996	40
CGATCGA	60	0.0	44.999996	41
TACGGCT	4530	0.0	43.956955	7
GATACCT	3705	0.0	43.42105	5
ACGGCTG	4620	0.0	43.149353	8
TGATACC	3735	0.0	43.012047	4
CGGCTGT	4785	0.0	42.695927	9
>>END_MODULE