FastQCFastQC Report
Sat 14 Jan 2017
SRR2933825.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933825.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences936138
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC455554.866269716644341No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG372223.9761231784202757No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC270992.894765515340687No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT134261.434190258273887No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC98751.0548658424292146Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA81430.8698503853064399No Hit
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT74090.7914431419299292No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC64970.6940216079253272No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT58460.6244805787180949No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.3464232837466271No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT30440.32516573411185107Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGT29560.31576541065526664No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATG27140.28991452114965954No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC26470.28275745669976005Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC19440.20766169090454611Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTAT19090.2039229258934046No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACGAAGG16120.1721968342274323No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT15680.16749667249914008No Hit
TGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCTT13440.14356857642783435Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACGCAGGTCGTATGCCGTCTTCTGC12810.13683879940777963Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG11280.12049505521621812No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGG207.0326135E-445.00000437
CGATCGA207.0326135E-445.00000441
TCGAATA302.1650685E-644.99999644
TACGGCT48950.044.1266637
ACGGCTG49450.043.8169868
GCGAACC1350.043.33333633
TGATACC42150.043.291824
GATACCT42600.042.9401445
CGGCTGT51950.042.574599
TAAGGCG1350.041.66666829
CGTTTTT16750.041.5074651
TCGTTCA556.002665E-1140.90909216
TTATACG1050.040.71428726
ATACCTG45150.040.564786
GCGACTG9850.040.43147311
ATGATAC162200.040.3113443
ACCACCT18300.040.20491814
AATGATC9600.040.078122
TTCGGGA7850.039.8407634
CCGGTGA2600.039.8076918
Produced by FastQC (version 0.11.5)