##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933825.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	936138
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.898790562929825	30.0	16.0	33.0	16.0	34.0
2	26.98126878729418	31.0	23.0	33.0	16.0	34.0
3	28.01958365112836	31.0	25.0	34.0	16.0	34.0
4	31.88809983143511	35.0	28.0	35.0	19.0	37.0
5	33.500082252830246	35.0	33.0	37.0	28.0	37.0
6	32.19420854617589	35.0	32.0	37.0	17.0	37.0
7	33.195397473449425	35.0	32.0	35.0	28.0	37.0
8	32.704442080120664	35.0	32.0	37.0	25.0	37.0
9	34.35366260102677	35.0	32.0	39.0	28.0	39.0
10	33.29779049670027	35.0	31.0	37.0	27.0	39.0
11	33.81728548568694	35.0	32.0	39.0	27.0	39.0
12	34.08916420442285	35.0	32.0	39.0	27.0	39.0
13	34.278962076104165	35.0	33.0	38.0	27.0	39.0
14	34.38963058865253	37.0	32.0	39.0	25.0	40.0
15	34.795048379619246	37.0	32.0	39.0	27.0	40.0
16	34.68536155994095	36.0	32.0	39.0	27.0	40.0
17	34.15963351557142	36.0	32.0	38.0	27.0	40.0
18	34.432825074935536	36.0	32.0	39.0	27.0	40.0
19	34.224903806917354	36.0	32.0	39.0	27.0	40.0
20	34.27567303111294	36.0	32.0	39.0	26.0	40.0
21	34.01087339687098	36.0	32.0	39.0	25.0	40.0
22	34.992978599309076	37.0	33.0	39.0	27.0	40.0
23	35.59685644637863	37.0	34.0	39.0	29.0	40.0
24	35.88946394655489	38.0	34.0	40.0	30.0	40.0
25	33.8806276425057	36.0	32.0	39.0	24.0	40.0
26	34.65107922122593	37.0	33.0	39.0	27.0	40.0
27	35.00722543043867	37.0	33.0	39.0	27.0	40.0
28	35.262558511672424	37.0	34.0	39.0	27.0	40.0
29	35.813105546404486	38.0	34.0	40.0	30.0	40.0
30	33.584280309099725	36.0	31.0	39.0	23.0	40.0
31	34.54198526285654	37.0	33.0	39.0	25.0	40.0
32	34.74662496341352	37.0	33.0	39.0	27.0	40.0
33	35.27359214132959	37.0	34.0	39.0	29.0	40.0
34	35.45200921231699	38.0	34.0	40.0	29.0	41.0
35	35.677077524894834	38.0	35.0	40.0	30.0	41.0
36	35.48943531829709	38.0	34.0	40.0	28.0	41.0
37	35.696620583717355	38.0	35.0	40.0	30.0	41.0
38	35.368899670775036	38.0	34.0	40.0	29.0	41.0
39	35.18492359032536	37.0	34.0	40.0	28.0	41.0
40	34.828985683734665	37.0	33.0	40.0	26.0	41.0
41	34.3899724185964	37.0	33.0	40.0	24.0	41.0
42	35.18658573842745	37.0	34.0	40.0	29.0	41.0
43	35.21637835447338	38.0	34.0	40.0	29.0	41.0
44	35.71675116275592	38.0	35.0	40.0	30.0	41.0
45	36.02438208896552	38.0	35.0	40.0	31.0	41.0
46	35.763160986948506	38.0	35.0	40.0	30.0	41.0
47	35.37972927068445	38.0	34.0	40.0	27.0	41.0
48	35.62728144782073	38.0	34.0	40.0	29.0	41.0
49	35.93679457515879	38.0	35.0	40.0	30.0	41.0
50	35.59250452390566	38.0	34.0	40.0	28.0	41.0
51	34.94064016202739	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	10.0
11	17.0
12	17.0
13	24.0
14	43.0
15	76.0
16	236.0
17	676.0
18	1443.0
19	2600.0
20	3947.0
21	4756.0
22	5381.0
23	5616.0
24	6642.0
25	8360.0
26	11143.0
27	14876.0
28	19786.0
29	26639.0
30	36423.0
31	49672.0
32	66838.0
33	85083.0
34	100106.0
35	120730.0
36	135254.0
37	135094.0
38	84395.0
39	10240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.411990539856305	3.207112626557196	42.24921966633124	14.131677167255255
2	16.43197904582444	24.74133087215774	41.28258867816497	17.544101403852853
3	16.41648987649257	24.784807368144442	42.0277779558142	16.770924799548784
4	15.76925624213524	3.461882756602125	60.312582119302924	20.45627888195971
5	32.39639882154127	4.304814033828346	40.88852284599066	22.41026429863973
6	18.047766461782345	21.994834094973175	43.84428364194168	16.1131158013028
7	66.79677568905439	1.541439403164918	27.474795382732033	4.1869895250486575
8	64.90154229397803	17.849718738049305	10.669473945080748	6.579265022891924
9	60.963234053099015	3.936064981872331	10.836650152007502	24.264050813021157
10	42.09144378286108	24.129134807047677	18.12478502101186	15.654636389079387
11	34.25136037635477	20.33183141801743	26.595010564681704	18.8217976409461
12	26.420143184017743	16.510279467343487	29.0205076601954	28.049069688443375
13	25.99061249516631	18.24720286966238	38.19415513524716	17.568029499924158
14	20.6514424155413	26.3586137941201	28.190822293294364	24.799121497044236
15	14.417211992249007	21.68173923075444	41.44175324578214	22.459295531214416
16	18.35701573913248	23.17938167236027	26.1142053842489	32.349397204258345
17	19.26051500953919	26.24901456836492	33.324787584736434	21.165682837359448
18	18.323153210317283	23.45220469631614	31.11955715930771	27.105084934058866
19	18.760161429190994	26.400487962244885	28.09948960516505	26.739861003399074
20	22.136800343539093	25.459066932439452	37.02691269876877	15.377220025252686
21	23.315793184338208	31.355526642439468	27.852837936287173	17.47584223693515
22	18.66060345803717	20.585746973202667	35.2915916243118	25.462057944448365
23	20.807829614864477	29.46456612166155	29.621807895844416	20.10579636762956
24	22.490807979165464	23.07298710232893	26.47216542860134	27.96403948990427
25	17.932612499439184	34.4197116237136	23.265800555046372	24.381875321800845
26	17.31507534145607	22.480980368279035	32.19012581478371	28.013818475481177
27	21.661443077836815	28.421237039838143	25.638314009259318	24.279005873065724
28	14.443169703612075	26.64040985410271	34.054914980483645	24.861505461801574
29	22.477562068840278	22.2295217158154	29.985429498642297	25.307486716702027
30	18.54074933396572	32.386143923225	28.667675065001102	20.405431677808185
31	23.425392410093384	23.56650408379961	23.68731960458821	29.320783901518794
32	30.156237648722733	29.576622250138335	22.301946935174087	17.965193165964845
33	22.387831708572882	26.1103597973803	21.80319568268781	29.69861281135901
34	24.999519301641424	25.33857187722323	28.853972384413407	20.807936436721935
35	24.68311295984139	27.22312308655348	23.840608970045015	24.253154983560115
36	22.166496819913302	26.52066255188872	26.86494939848612	24.44789122971186
37	23.37572024637393	29.543934761755214	27.73757715208655	19.342767839784305
38	23.204164343291268	26.670106330476916	20.967314648054025	29.15841467817779
39	28.241135388158582	27.646671751387082	24.172931768606766	19.93926109184757
40	24.47951049952037	28.021936936648228	26.227329731300298	21.2712228325311
41	23.91784117298945	29.67479153714516	22.56515599195845	23.842211297906932
42	26.697666369701906	20.63093261890875	29.928066161185633	22.74333485020371
43	28.22115970081334	21.3667215731014	25.33248303134794	25.079635694737313
44	22.661722951103364	23.347519276004178	29.428246690124748	24.562511082767713
45	20.220523042542872	18.878947334687833	27.246837538909862	33.65369208385943
46	29.831712845755646	24.84441396460778	24.48228786781436	20.84158532182221
47	16.523311733953754	23.590859467300763	38.83754318273588	21.048285616009604
48	21.537957010611684	27.6378055372178	25.381086976492785	25.443150475677733
49	22.876007597170503	19.47116771245265	34.624595946324156	23.028228744052694
50	25.72783072581179	20.129724463700864	27.804554456714715	26.337890353772625
51	20.492598313496515	20.391972123768078	26.622463782049227	32.49296578068618
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	398.5
2	677.0
3	3329.0
4	5981.0
5	4090.5
6	2200.0
7	2115.0
8	2030.0
9	2156.0
10	2282.0
11	2199.0
12	2116.0
13	2035.5
14	1955.0
15	1913.5
16	1872.0
17	1760.5
18	1649.0
19	1731.5
20	1814.0
21	2182.5
22	2551.0
23	2754.0
24	2957.0
25	3829.0
26	5669.5
27	6638.0
28	7769.5
29	8901.0
30	10467.0
31	12033.0
32	14147.5
33	16262.0
34	17969.0
35	19676.0
36	21211.5
37	22747.0
38	24687.5
39	26628.0
40	29848.0
41	33068.0
42	35915.0
43	38762.0
44	43066.0
45	47370.0
46	126423.5
47	205477.0
48	155053.5
49	104630.0
50	96003.5
51	87377.0
52	72844.5
53	58312.0
54	52027.5
55	45743.0
56	43460.0
57	41177.0
58	39192.0
59	37207.0
60	33407.5
61	29608.0
62	25840.5
63	22073.0
64	18210.5
65	14348.0
66	12077.0
67	9806.0
68	7771.5
69	5737.0
70	5010.5
71	4284.0
72	3322.0
73	2360.0
74	1932.0
75	1180.5
76	857.0
77	621.5
78	386.0
79	255.0
80	124.0
81	114.0
82	104.0
83	60.5
84	17.0
85	10.5
86	4.0
87	5.0
88	6.0
89	6.5
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	936138.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.920820525496225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30040789723363	21.199079412415596
2	10.2091870233527	5.905161312272169
3	3.635366815536293	3.1541337364940962
4	1.8086354282862878	2.0922888247008697
5	1.224670872019095	1.7709243246233606
6	0.8982326745070527	1.5586575581732967
7	0.7463284660402583	1.510910213359345
8	0.6780498486686363	1.5687806384548406
9	0.6273784756780721	1.6329870266980433
>10	6.7193368846169275	33.907505812751275
>50	0.08724940709227928	1.7161854979501494
>100	0.05354791722146717	3.1210164898237758
>500	0.0037446095959067847	0.6690599907745327
>1k	0.004493531515088142	2.7496654525356727
>5k	0.0018723047979533924	4.090384566454208
>10k+	0.0014978438383627139	13.353259142518844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGC	45555	4.866269716644341	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCG	37222	3.9761231784202757	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTC	27099	2.894765515340687	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGAAGGT	13426	1.434190258273887	No Hit
GCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	9875	1.0548658424292146	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTA	8143	0.8698503853064399	No Hit
GAACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCT	7409	0.7914431419299292	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTC	6497	0.6940216079253272	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCT	5846	0.6244805787180949	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3243	0.3464232837466271	No Hit
CTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCT	3044	0.32516573411185107	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACGAAGGTCGT	2956	0.31576541065526664	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATG	2714	0.28991452114965954	No Hit
CCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	2647	0.28275745669976005	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGC	1944	0.20766169090454611	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTAT	1909	0.2039229258934046	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACGAAGG	1612	0.1721968342274323	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTT	1568	0.16749667249914008	No Hit
TGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTGCTT	1344	0.14356857642783435	Illumina Single End Adapter 1 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTACGCAGGTCGTATGCCGTCTTCTGC	1281	0.13683879940777963	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTACGAAGGTCGTATGCCGTCTTCTG	1128	0.12049505521621812	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.39577498189369514	0.0
2	0.0	0.0	0.0	2.2636619814599985	0.0
3	0.0	0.0	0.0	2.76358827437835	0.0
4	0.0	0.0	0.0	4.19713760150747	0.0
5	0.0	0.0	0.0	8.253056707451252	0.0
6	0.0	0.0	0.0	8.935968842200616	0.0
7	0.0	0.0	0.0	10.115495792287035	0.0
8	0.0	0.0	0.0	11.558445442872738	0.0
9	0.0	0.0	0.0	11.926553563683987	0.0
10	0.0	0.0	0.0	16.87881487558458	0.0
11	0.0	0.0	0.0	18.217079105858325	0.0
12	0.0	0.0	0.0	24.643802516295676	0.0
13	0.0	0.0	0.0	25.338999164653075	0.0
14	0.0	0.0	0.0	25.68232461453333	0.0
15	0.0	0.0	0.0	26.90928046933251	0.0
16	0.0	0.0	0.0	27.670493025600926	0.0
17	0.0	0.0	0.0	28.362805483806873	0.0
18	0.0	0.0	0.0	28.985897378377974	0.0
19	1.0682185746118628E-4	0.0	0.0	31.127141511187453	0.0
20	2.1364371492237256E-4	0.0	0.0	31.866455586676324	0.0
21	2.1364371492237256E-4	0.0	0.0	32.58771676825425	0.0
22	2.1364371492237256E-4	0.0	0.0	33.47797012833578	0.0
23	2.1364371492237256E-4	0.0	0.0	34.20767023665314	0.0
24	2.1364371492237256E-4	0.0	0.0	34.76602808560276	0.0
25	2.1364371492237256E-4	0.0	0.0	35.25463126163023	0.0
26	2.1364371492237256E-4	0.0	0.0	35.72539518746168	0.0
27	2.1364371492237256E-4	0.0	0.0	36.25576570975647	0.0
28	2.1364371492237256E-4	0.0	0.0	36.71937257113802	0.0
29	2.1364371492237256E-4	0.0	0.0	37.22816507822564	0.0
30	2.1364371492237256E-4	0.0	0.0	37.827969807870204	0.0
31	2.1364371492237256E-4	0.0	0.0	38.34391937940774	0.0
32	2.1364371492237256E-4	0.0	0.0	38.821840369689085	0.0
33	2.1364371492237256E-4	0.0	0.0	39.30841393042479	0.0
34	2.1364371492237256E-4	0.0	0.0	39.82393621453247	0.0
35	2.1364371492237256E-4	0.0	0.0	40.33700159591855	0.0
36	2.1364371492237256E-4	0.0	0.0	40.85231023631131	0.0
37	2.1364371492237256E-4	0.0	0.0	41.37328043514952	0.0
38	2.1364371492237256E-4	0.0	0.0	41.93067688738199	0.0
39	2.1364371492237256E-4	0.0	0.0	42.78129933834541	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGG	20	7.0326135E-4	45.000004	37
CGATCGA	20	7.0326135E-4	45.000004	41
TCGAATA	30	2.1650685E-6	44.999996	44
TACGGCT	4895	0.0	44.126663	7
ACGGCTG	4945	0.0	43.816986	8
GCGAACC	135	0.0	43.333336	33
TGATACC	4215	0.0	43.29182	4
GATACCT	4260	0.0	42.940144	5
CGGCTGT	5195	0.0	42.57459	9
TAAGGCG	135	0.0	41.666668	29
CGTTTTT	1675	0.0	41.507465	1
TCGTTCA	55	6.002665E-11	40.909092	16
TTATACG	105	0.0	40.714287	26
ATACCTG	4515	0.0	40.56478	6
GCGACTG	985	0.0	40.431473	11
ATGATAC	16220	0.0	40.311344	3
ACCACCT	1830	0.0	40.204918	14
AATGATC	960	0.0	40.07812	2
TTCGGGA	785	0.0	39.840763	4
CCGGTGA	260	0.0	39.80769	18
>>END_MODULE