FastQCFastQC Report
Sat 14 Jan 2017
SRR2933822.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933822.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences698741
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC366845.250013953668097No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG304654.359984600875003No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC232093.3215454653440974No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT118641.6979109569926483No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC99281.4208411986701797No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA67330.9635902287113538No Hit
GAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT62170.889743123703919No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC53930.7718167389633641No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT47640.6817976904174794No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC36840.5272339822623834No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT35580.5092015496442888No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACAACCATCGT23170.33159639981051636No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG22900.3277323071066389No Hit
TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC21480.3074100417751356No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20500.2933848164055065No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT16500.23613899857028572No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAACCATCGTAT15130.2165323059617226No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG14310.20479691330550234No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGACAACCA12930.18504710615235115No Hit
TGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCTT10400.148839126371574Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC7570.1083377102531553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG351.2110104E-745.00000420
CTTCGAA207.0313853E-445.038
GCGCGAA253.8892304E-545.018
CGCGCGA406.8084773E-945.041
TACGGCT40850.043.9534877
TGATACC35350.043.7906654
GATACCT35650.043.422165
ACGGCTG41500.043.3192758
CGGCGAA1000.042.7531
CGTTTTT13300.042.4624061
CGGCTGT43350.041.989629
GGCGACT7750.041.80645410
ATACCTG37100.041.7250676
ACCACCT16850.041.6617214
GAATCTG29800.040.9983251
ACCGGTG2800.040.98214317
GCGACTG7750.040.93548611
GGCGAAC1050.040.71428732
CACCGGT3100.040.64516416
CGGGATC1500.040.5000046
Produced by FastQC (version 0.11.5)