##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933822.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	698741
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.522901904997703	30.0	16.0	31.0	16.0	34.0
2	26.50100967311207	30.0	16.0	33.0	16.0	34.0
3	27.566199779317373	31.0	25.0	33.0	16.0	34.0
4	31.584694471914485	35.0	28.0	35.0	19.0	37.0
5	33.33990992370563	35.0	33.0	37.0	28.0	37.0
6	31.903439185620996	35.0	31.0	37.0	17.0	37.0
7	33.08663868300272	35.0	32.0	35.0	28.0	37.0
8	32.41088328865774	35.0	32.0	37.0	23.0	37.0
9	34.26234613397525	35.0	32.0	39.0	28.0	39.0
10	33.406004513832734	35.0	32.0	37.0	27.0	39.0
11	33.98161121216588	35.0	32.0	39.0	27.0	39.0
12	34.253564625519324	35.0	32.0	39.0	27.0	39.0
13	34.48257938206002	35.0	33.0	38.0	27.0	39.0
14	34.48535151078869	37.0	32.0	39.0	27.0	40.0
15	34.96227929948293	37.0	33.0	39.0	27.0	40.0
16	34.73823204878489	36.0	32.0	39.0	27.0	40.0
17	34.14561618682745	36.0	32.0	38.0	27.0	40.0
18	34.43590400448807	36.0	32.0	39.0	27.0	40.0
19	34.22201502416489	36.0	32.0	39.0	27.0	40.0
20	34.26656944418605	36.0	32.0	39.0	26.0	40.0
21	33.97892638330941	36.0	32.0	39.0	25.0	40.0
22	35.03009269529053	37.0	33.0	39.0	27.0	40.0
23	35.560493802424645	37.0	34.0	39.0	29.0	40.0
24	35.8550249663323	38.0	34.0	40.0	30.0	40.0
25	33.86884267561228	36.0	32.0	39.0	24.0	40.0
26	34.74248970648638	37.0	33.0	39.0	27.0	40.0
27	34.88653449561426	37.0	33.0	39.0	27.0	40.0
28	35.081754183595926	37.0	33.0	39.0	27.0	40.0
29	35.510478131382015	38.0	34.0	40.0	28.0	40.0
30	33.18837165702313	36.0	31.0	39.0	22.0	40.0
31	34.095461694676565	36.0	31.0	39.0	25.0	40.0
32	34.482915701239804	37.0	33.0	39.0	26.0	40.0
33	35.05497315886716	37.0	34.0	39.0	27.0	40.0
34	35.344861686948384	38.0	34.0	40.0	29.0	40.0
35	35.34528530600036	38.0	34.0	40.0	27.0	41.0
36	35.539726450859476	38.0	35.0	40.0	29.0	41.0
37	35.359493717987064	38.0	35.0	40.0	27.0	41.0
38	35.1874256698834	37.0	34.0	40.0	28.0	41.0
39	35.12410893306676	37.0	34.0	40.0	28.0	41.0
40	34.57998886568843	37.0	33.0	40.0	26.0	41.0
41	34.146062704206564	37.0	33.0	39.0	24.0	40.0
42	35.04039837364631	37.0	34.0	40.0	27.0	41.0
43	35.20803273315864	38.0	34.0	40.0	28.0	40.0
44	35.70363696992162	38.0	35.0	40.0	29.0	41.0
45	35.88838782896667	38.0	35.0	40.0	30.0	41.0
46	35.58853709743668	38.0	35.0	40.0	29.0	41.0
47	35.157687898663454	37.0	34.0	40.0	26.0	41.0
48	35.26695299116554	37.0	34.0	40.0	28.0	41.0
49	35.536105652881396	38.0	34.0	40.0	29.0	41.0
50	35.320041331480475	37.0	34.0	40.0	28.0	41.0
51	34.78218825000966	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	10.0
11	23.0
12	20.0
13	25.0
14	39.0
15	61.0
16	173.0
17	429.0
18	1100.0
19	2170.0
20	3439.0
21	4274.0
22	4770.0
23	4764.0
24	5287.0
25	6316.0
26	8460.0
27	11268.0
28	15085.0
29	20442.0
30	27517.0
31	37860.0
32	51006.0
33	64984.0
34	77842.0
35	91263.0
36	99358.0
37	95902.0
38	58030.0
39	6813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52491266434916	3.5005817606237506	39.43335227215807	14.541153302869017
2	15.673475579649685	27.953705307116657	38.44614814358969	17.926670969643972
3	16.374164389952785	27.862684456758657	39.79900993358054	15.964141219708017
4	15.607070430960828	3.4536401899988696	61.04808505583614	19.89120432320416
5	34.0177547904016	4.648503522764515	38.550335532049786	22.783406154784107
6	17.038502105930522	24.36897219427513	42.75647199749263	15.836053702301712
7	62.683168727754634	1.8639238287147883	30.71137946678383	4.741527976746749
8	59.66903330418567	20.000973178903198	12.139691244681504	8.190302272229625
9	55.233197994679	4.430969414990677	12.654188032475552	27.681644557854767
10	40.78277931307881	22.032627253875184	20.509602270369136	16.67499116267687
11	33.41252338133872	20.48155181962988	27.25101289318932	18.85491190584208
12	24.015193040053468	16.526152036305298	30.088688083281216	29.369966840360018
13	24.99237915050069	19.541718605320142	39.02418778918083	16.441714454998348
14	20.295646026209997	28.590851259622664	26.068171182169074	25.045331531998265
15	13.638959213785936	22.260179379770186	41.38271548399192	22.718145922451953
16	15.80013195161011	24.4124790158299	25.29463706867065	34.49275196388934
17	16.339101326528713	28.20272461469987	34.07056405735459	21.387610001416835
18	16.060457308215774	23.436724050828563	32.028176391538494	28.474642249417165
19	16.01394508122466	27.332445069059926	28.385911231772575	28.267698617942845
20	20.817441655778033	24.902216987410213	38.792199112403594	15.488142244408158
21	22.071554410003134	33.1109810358917	27.240565531434395	17.576899022670776
22	17.521084350281434	20.52835027571017	35.3149450225477	26.635620351460698
23	20.121904969080102	30.40354008137493	28.64280183930813	20.83175311023684
24	22.086295208095706	22.68751940991011	26.115828325516894	29.110357056477294
25	16.03083259748605	35.664287625887134	23.045735115014004	25.259144661612815
26	19.633168799311907	21.97724192511961	30.73256041938286	27.657028856185622
27	21.62589571815594	29.97662939486877	23.96596163671518	24.43151325026011
28	14.611565658806338	27.166145968248607	33.04772440718378	25.174563965761276
29	24.956886743442848	23.451894192554896	25.78838224749943	25.802836816502822
30	17.067125014848134	34.524952736421646	27.37366205790128	21.034260190828935
31	21.970944885157735	23.24366253017928	20.730857356302263	34.05453522836072
32	23.354433187690432	35.981715685783435	19.861007154296082	20.802843972230054
33	17.901482809796477	28.674430153662083	20.327131226019368	33.09695581052207
34	26.195256897763265	23.121299594556497	23.01353434248169	27.669909165198547
35	17.83064111022539	29.730615492721913	21.934736905376955	30.504006491675746
36	23.318940780632595	29.661204938596704	19.88562285596523	27.13423142480547
37	15.93895305986052	33.34754937809575	27.74618921746398	22.967308344579752
38	17.860552050044294	30.276311251236155	19.853565197977506	32.00957150074205
39	20.486131485056696	31.712608820721844	23.450892390742776	24.350367303478684
40	18.151360804647215	28.543623459908606	25.14107516232767	28.163940573116502
41	17.452246254334582	29.482597986950815	21.076765210571587	31.98839054814302
42	17.857689759152535	21.928439865415083	29.462132607074725	30.75173776835766
43	23.44359354896879	28.58283684512573	21.822105758786158	26.151463847119317
44	20.334000724159594	23.46964039608381	29.276799271833198	26.919559607923393
45	18.058050121575807	18.86221074761607	26.87490787001192	36.204831260796205
46	26.941169904156187	28.34211818112863	23.387921991124035	21.328789923591145
47	14.330488693235404	24.48518120448063	39.221828975256926	21.96250112702704
48	20.554254008280605	25.311095241298275	24.5490102913669	29.585640459054215
49	18.53533712777696	19.40346995524808	35.75387733079925	26.307315586175704
50	22.89761156136537	22.050373457404103	27.601786641974634	27.45022833925589
51	19.90980921400061	19.191946658346943	26.108958827376664	34.78928530027578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	230.0
2	384.0
3	2140.5
4	3897.0
5	2740.5
6	1584.0
7	1623.0
8	1662.0
9	1838.5
10	2015.0
11	1959.0
12	1903.0
13	1942.5
14	1982.0
15	1838.0
16	1694.0
17	1664.0
18	1634.0
19	1707.0
20	1780.0
21	1899.5
22	2019.0
23	2420.5
24	2822.0
25	3441.0
26	4674.0
27	5288.0
28	5934.5
29	6581.0
30	7383.0
31	8185.0
32	9613.0
33	11041.0
34	12722.0
35	14403.0
36	15396.0
37	16389.0
38	17539.0
39	18689.0
40	20742.0
41	22795.0
42	25382.0
43	27969.0
44	32194.0
45	36419.0
46	101028.0
47	165637.0
48	125673.0
49	85709.0
50	78117.0
51	70525.0
52	56591.5
53	42658.0
54	36819.5
55	30981.0
56	28613.5
57	26246.0
58	24494.5
59	22743.0
60	20754.0
61	18765.0
62	16366.5
63	13968.0
64	11635.0
65	9302.0
66	7772.0
67	6242.0
68	5312.5
69	4383.0
70	3582.0
71	2781.0
72	2191.5
73	1602.0
74	1320.0
75	759.5
76	481.0
77	392.0
78	303.0
79	176.0
80	49.0
81	33.0
82	17.0
83	16.5
84	16.0
85	19.0
86	22.0
87	11.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	698741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.635621088399294
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48741498231736	21.329933912879966
2	10.1382003851319	5.806273294938017
3	3.7060548565126714	3.183755478117566
4	1.7509436593581844	2.00557436706065
5	1.1213501325682556	1.6055278751825441
6	0.7650118935300719	1.3143954426747602
7	0.6463669681779756	1.2956383709361368
8	0.5592852502343623	1.2812384404833423
9	0.5054133738113369	1.3025543278923861
>10	6.175433029804931	32.48912014461645
>50	0.07657314298770966	1.51065304921978
>100	0.05426043907074096	3.2763004995181233
>500	0.003549748350422306	0.6218029757528856
>1k	0.006085282886438239	4.027924087210074
>5k	0.002028427628812746	4.105322729451151
>10k+	0.002028427628812746	14.843985004066202
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	36684	5.250013953668097	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCG	30465	4.359984600875003	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTC	23209	3.3215454653440974	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACAACCAT	11864	1.6979109569926483	No Hit
GCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	9928	1.4208411986701797	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACAACCATCGTA	6733	0.9635902287113538	No Hit
GAACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCT	6217	0.889743123703919	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTC	5393	0.7718167389633641	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCT	4764	0.6817976904174794	No Hit
CCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	3684	0.5272339822623834	No Hit
CTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCT	3558	0.5092015496442888	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGACAACCATCGT	2317	0.33159639981051636	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGACAACCATCGTATG	2290	0.3277323071066389	No Hit
TCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGC	2148	0.3074100417751356	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2050	0.2933848164055065	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTT	1650	0.23613899857028572	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGACAACCATCGTAT	1513	0.2165323059617226	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTG	1431	0.20479691330550234	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGACAACCA	1293	0.18504710615235115	No Hit
TGTCTCTTATACACATCTGACGCGACAACCATCGTATGCCGTCTTCTGCTT	1040	0.148839126371574	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGACAACCATCGTATGC	757	0.1083377102531553	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5866265182664249	0.0
2	0.0	0.0	0.0	3.15166850091808	0.0
3	0.0	0.0	0.0	3.833036847701795	0.0
4	0.0	0.0	0.0	5.556135964541941	0.0
5	0.0	0.0	0.0	10.385250042576576	0.0
6	0.0	0.0	0.0	11.21302456847387	0.0
7	0.0	0.0	0.0	12.579052896566825	0.0
8	0.0	0.0	0.0	14.260935024565612	0.0
9	0.0	0.0	0.0	14.647916753131703	0.0
10	0.0	0.0	0.0	20.128917581764917	0.0
11	0.0	0.0	0.0	21.52686045330101	0.0
12	0.0	0.0	0.0	28.561226548892936	0.0
13	0.0	0.0	0.0	29.315869542505734	0.0
14	0.0	0.0	0.0	29.714157320094284	0.0
15	0.0	0.0	0.0	31.071885004601132	0.0
16	0.0	0.0	0.0	31.924704575801336	0.0
17	0.0	0.0	0.0	32.7228543909689	0.0
18	0.0	0.0	0.0	33.366869841615134	0.0
19	0.0	0.0	0.0	35.8418927757209	0.0
20	0.0	0.0	0.0	36.60540887109816	0.0
21	0.0	0.0	0.0	37.33858468302275	0.0
22	0.0	0.0	0.0	38.209866030474814	0.0
23	0.0	0.0	0.0	38.88221816094948	0.0
24	0.0	0.0	0.0	39.47285188646437	0.0
25	0.0	0.0	0.0	39.95357364173564	0.0
26	0.0	0.0	0.0	40.45790929686393	0.0
27	0.0	0.0	0.0	40.98385524822502	0.0
28	0.0	0.0	0.0	41.39745628208449	0.0
29	0.0	0.0	0.0	41.85399167932038	0.0
30	0.0	0.0	0.0	42.38151189067194	0.0
31	0.0	0.0	0.0	42.851213826009925	0.0
32	0.0	0.0	0.0	43.296300059678764	0.0
33	0.0	0.0	0.0	43.764141505937104	0.0
34	0.0	0.0	0.0	44.22482722496605	0.0
35	0.0	0.0	0.0	44.68007459130064	0.0
36	0.0	0.0	0.0	45.13789801943782	0.0
37	0.0	0.0	0.0	45.59242981304947	0.0
38	0.0	0.0	0.0	46.05454667752429	0.0
39	0.0	0.0	0.0	46.55415955268118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAAAG	35	1.2110104E-7	45.000004	20
CTTCGAA	20	7.0313853E-4	45.0	38
GCGCGAA	25	3.8892304E-5	45.0	18
CGCGCGA	40	6.8084773E-9	45.0	41
TACGGCT	4085	0.0	43.953487	7
TGATACC	3535	0.0	43.790665	4
GATACCT	3565	0.0	43.42216	5
ACGGCTG	4150	0.0	43.319275	8
CGGCGAA	100	0.0	42.75	31
CGTTTTT	1330	0.0	42.462406	1
CGGCTGT	4335	0.0	41.98962	9
GGCGACT	775	0.0	41.806454	10
ATACCTG	3710	0.0	41.725067	6
ACCACCT	1685	0.0	41.66172	14
GAATCTG	2980	0.0	40.998325	1
ACCGGTG	280	0.0	40.982143	17
GCGACTG	775	0.0	40.935486	11
GGCGAAC	105	0.0	40.714287	32
CACCGGT	310	0.0	40.645164	16
CGGGATC	150	0.0	40.500004	6
>>END_MODULE