SRR2933821.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933821.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408783
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	30466	7.452853959191062	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	26643	6.517638942911031	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	23451	5.736784553173688	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	12951	3.168184586932431	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAACAAT	10405	2.5453602522609793	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	7599	1.8589324898540303	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTA	6940	1.6977222634013645	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC	5973	1.4611664379389555	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	5690	1.391936553134548	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	5505	1.3466802680150594	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4523	1.1064550140294485	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4119	1.0076250724712135	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAACAATCGT	2477	0.6059449634647233	No Hit
TGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCTT	2192	0.5362258215238892	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATG	2062	0.5044241076561403	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT	1705	0.4170917088039376	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	1426	0.3488403382723841	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAACAA	1243	0.3040733102893222	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1194	0.29208651044686296	No Hit
GCTGTCTCTTATACACATCTGACGCCGGACCAATCGTATGCCGTCTTCTGC	1184	0.2896402247647285	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1169	0.28597079624152666	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	952	0.23288639693920735	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	873	0.21356074005034453	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	763	0.18665159754686472	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	760	0.18591771184222436	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCGGAACAATCG	726	0.17760034052296694	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	715	0.17490942627261896	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	690	0.16879371206728264	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	657	0.1607209693162387	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	600	0.14677714092807187	No Hit
TTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	585	0.14310771240487005	No Hit
GCACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	567	0.13870439817702793	No Hit
TGTCTCTTATACACATCTGACGCCGGAACCATCGTATGCCGTCTTCTGCTT	561	0.13723662676774717	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	542	0.1325886839716916	No Hit
TTGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	537	0.13136554113062432	No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	520	0.1272068554709956	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	494	0.12084651269744584	No Hit
GCCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	467	0.11424154135568261	No Hit
GCATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	450	0.1100828556960539	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	448	0.10959359855962698	No Hit
GACTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	446	0.10910434142320008	No Hit
TGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	432	0.10567954146821174	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	421	0.10298862721786375	No Hit
TCTGTCTCTTATACACATCTGACGCCGGACCAATCGTATGCCGTCTTCTGC	413	0.10103159867215614	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCTT	20	7.0279464E-4	45.000004	18
CCTTAAG	20	7.0279464E-4	45.000004	25
ACCGGTG	40	6.7957444E-9	45.000004	17
CGACGAA	20	7.0279464E-4	45.000004	19
GACCGTG	20	7.0279464E-4	45.000004	15
TTCACCC	20	7.0279464E-4	45.000004	22
ACCGACG	20	7.0279464E-4	45.000004	17
TTCGTTG	40	6.7957444E-9	45.000004	1
TTCGTCG	20	7.0279464E-4	45.000004	1
GCGTTGG	40	6.7957444E-9	45.000004	2
GCACCGA	65	0.0	45.000004	9
TTTCACC	20	7.0279464E-4	45.000004	21
TAGTTGG	20	7.0279464E-4	45.000004	2
CTCGGGT	105	0.0	45.000004	4
ATGAGAC	20	7.0279464E-4	45.000004	11
TCTCGTG	20	7.0279464E-4	45.000004	1
TCTAACC	20	7.0279464E-4	45.000004	24
CGGGCTA	20	7.0279464E-4	45.000004	6
AGACCGT	20	7.0279464E-4	45.000004	14
TCATGAC	20	7.0279464E-4	45.000004	36

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content