##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933821.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	408783
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.826458047423696	25.0	16.0	31.0	16.0	33.0
2	24.587069423141372	28.0	16.0	31.0	16.0	34.0
3	25.67425749113833	30.0	16.0	33.0	16.0	34.0
4	30.41380634713283	32.0	28.0	35.0	19.0	37.0
5	33.03214174757757	35.0	32.0	35.0	28.0	37.0
6	31.10155265752245	35.0	28.0	35.0	17.0	37.0
7	33.12454774293452	35.0	32.0	35.0	28.0	37.0
8	31.56980353879687	35.0	28.0	36.0	17.0	37.0
9	34.0957256050276	35.0	32.0	38.0	28.0	39.0
10	33.896319073934094	35.0	32.0	38.0	28.0	39.0
11	34.67360188657552	35.0	33.0	39.0	28.0	39.0
12	34.70923937639285	37.0	34.0	39.0	27.0	39.0
13	34.89918367446787	37.0	34.0	39.0	30.0	39.0
14	34.55299266358924	36.0	33.0	39.0	27.0	40.0
15	35.2624644371219	37.0	34.0	39.0	27.0	40.0
16	34.42649278468038	36.0	32.0	38.0	27.0	40.0
17	34.06399483344464	36.0	32.0	38.0	27.0	40.0
18	34.44208051704694	36.0	32.0	39.0	27.0	40.0
19	33.89887299618624	35.0	32.0	39.0	27.0	40.0
20	34.04550825254475	36.0	32.0	39.0	25.0	40.0
21	34.07423743159573	36.0	32.0	39.0	26.0	40.0
22	35.44573037528469	37.0	34.0	39.0	30.0	40.0
23	35.69846593424873	37.0	34.0	39.0	30.0	40.0
24	35.93952292536627	38.0	34.0	39.0	30.0	40.0
25	33.9898943938471	36.0	32.0	39.0	25.0	40.0
26	35.184687230144114	37.0	33.0	39.0	28.0	40.0
27	35.01501530151694	37.0	33.0	39.0	28.0	40.0
28	35.134643563944685	37.0	33.0	39.0	27.0	40.0
29	35.474868084044594	38.0	34.0	40.0	27.0	40.0
30	33.13710941991228	36.0	31.0	39.0	21.0	40.0
31	34.225070514184786	36.0	31.0	39.0	25.0	40.0
32	34.4639258481884	36.0	33.0	39.0	26.0	40.0
33	34.77287460584222	37.0	33.0	39.0	27.0	40.0
34	35.368576971155846	38.0	34.0	40.0	30.0	40.0
35	35.544371952845395	38.0	34.0	40.0	29.0	40.0
36	35.62675306947696	38.0	35.0	40.0	30.0	40.0
37	35.72457758761984	38.0	35.0	40.0	30.0	40.0
38	35.297025561239096	37.0	34.0	40.0	30.0	40.0
39	35.310962050770215	37.0	34.0	40.0	29.0	40.0
40	34.56581120056362	37.0	33.0	39.0	26.0	40.0
41	34.05244591873928	37.0	32.0	39.0	25.0	40.0
42	34.98499692991147	37.0	33.0	39.0	28.0	40.0
43	35.294794548696984	37.0	34.0	40.0	28.0	40.0
44	35.82092454921071	38.0	35.0	40.0	30.0	40.0
45	36.06709672368959	38.0	35.0	40.0	31.0	41.0
46	35.696095483422745	38.0	35.0	40.0	30.0	41.0
47	34.97626858259761	37.0	33.0	40.0	26.0	41.0
48	35.36131639525127	37.0	34.0	40.0	29.0	41.0
49	35.73205588295012	38.0	34.0	40.0	30.0	41.0
50	35.50690953390919	38.0	34.0	40.0	28.0	41.0
51	35.011509774134446	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	7.0
11	7.0
12	4.0
13	12.0
14	10.0
15	27.0
16	55.0
17	139.0
18	328.0
19	614.0
20	940.0
21	1331.0
22	1721.0
23	2170.0
24	2805.0
25	3704.0
26	5212.0
27	6814.0
28	9330.0
29	12476.0
30	17281.0
31	23734.0
32	32241.0
33	42683.0
34	52361.0
35	61799.0
36	61780.0
37	47242.0
38	20435.0
39	1518.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.5694977530866	3.1992524150955397	31.483941357639626	11.747308474178233
2	14.14662547121578	39.34752668286108	28.698845108529465	17.807002737393677
3	12.606688634312091	39.02999880132001	34.029056981332396	14.3342555830355
4	15.780010421177007	2.926246932969326	63.37641242419573	17.917330221657945
5	39.41039622489193	3.4358082405579493	35.103710281494095	22.05008525305602
6	14.2997629549174	30.989547021280238	37.22806476785972	17.482625255942636
7	46.825332756009914	1.1318963851236474	44.592607813925724	7.450163044940713
8	38.96541685931167	25.627044177473135	19.52356140054748	15.883977562667722
9	36.474119520625855	3.875895034773951	20.886142525496414	38.76384291910378
10	36.127236210899184	16.481605154813188	28.02685043164711	19.36430820264052
11	29.005853961637346	20.00670282276905	30.09078166166401	20.89666155392959
12	17.602737882935447	15.169172886348015	35.80726204367598	31.420827187040558
13	18.50076935684703	23.080705410939302	45.7360017417554	12.682523490458264
14	16.99165571953824	32.350171117683466	23.85568871503952	26.802484447738777
15	7.140707906150697	25.679150062502597	46.78741532793683	20.392726703409878
16	7.490037501559507	27.542241238016256	23.597116318437898	41.370604941986336
17	9.556170388690333	30.96777507870924	35.16780296636602	24.308251566234407
18	8.35455486162585	25.54876303564483	35.962356555923314	30.134325546806007
19	9.213935021759712	28.748749336445005	28.888676877463105	33.148638764332176
20	11.462316192209558	27.21492821374666	46.53593715981339	14.786818434230387
21	17.426360685253545	36.206985124136764	27.653302607985164	18.713351582624522
22	10.70152134506572	23.26564460850867	34.67585491568877	31.356979130736846
23	13.176428569681226	35.204497251598035	28.360523798690256	23.258550380030478
24	21.962263597067395	22.014369482096857	22.296915478383397	33.72645144245235
25	10.329441292813058	40.66729780837266	18.60767204115631	30.395588857657973
26	15.120491801273536	20.00523505135977	28.24897317158492	36.62529997578177
27	26.85507958990467	27.670426607760106	18.900492437307815	26.57400136502741
28	15.355090598190237	25.43329835144808	31.431590844041953	27.780020206319733
29	22.67853604479638	22.639884731018658	19.06243654946512	35.61914267471984
30	24.79799795979774	34.42486600470176	22.685141016138147	18.09199501936235
31	28.03321077442066	25.060973670627202	13.790446275896992	33.115369279055145
32	20.66695532837716	48.04798633994075	13.0705043996448	18.21455393203729
33	15.762886421402063	33.855370697900845	14.370215982562875	36.011526898134214
34	22.437576905106134	26.541710394023234	18.113522333365133	32.90719036750549
35	18.738792953718722	34.8218981709122	16.031243960732223	30.408064914636864
36	24.160006653897057	30.36770120088164	15.88862550546378	29.58366663975752
37	23.048414439935126	28.401621398150116	29.889941607160768	18.66002255475399
38	26.707568563271955	36.38067140756832	14.83525489073665	22.076505138423073
39	19.609915285126828	34.16776137950942	23.102966610646725	23.119356724717026
40	17.853726793922448	36.6020602618015	23.866941629177337	21.67727131509872
41	18.134071133095063	33.758742413456524	15.732063221807168	32.37512323164124
42	18.034018048695764	28.876200820484215	30.177869431948	22.911911698872018
43	24.77598138865853	28.85834293500464	17.76884068075238	28.59683499558445
44	23.00266889767921	17.94962119266212	29.734113209208797	29.313596700449875
45	20.74939515586509	15.694879679438726	25.344742809754813	38.21098235494137
46	31.03284627785402	26.874405246793533	24.175662882262717	17.91708559308973
47	11.187598310105852	19.80194871117439	47.644593830956765	21.365859147762993
48	20.080091393233086	23.452051577487325	22.782992443423527	33.68486458585607
49	16.943219263031974	16.768799093895783	43.27454908839164	23.013432554680602
50	25.24933766815156	16.14646401636076	29.736804123459148	28.867394192028534
51	18.380656729854223	14.324225811738748	25.838892517545986	41.45622494086104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54.0
1	110.5
2	167.0
3	658.0
4	1149.0
5	908.0
6	667.0
7	950.0
8	1233.0
9	1640.0
10	2047.0
11	2067.0
12	2087.0
13	1953.0
14	1819.0
15	1651.0
16	1483.0
17	1465.0
18	1447.0
19	1432.5
20	1418.0
21	1416.0
22	1414.0
23	1592.5
24	1771.0
25	1871.5
26	2094.0
27	2216.0
28	2460.5
29	2705.0
30	2764.0
31	2823.0
32	2931.0
33	3039.0
34	3634.0
35	4229.0
36	4494.0
37	4759.0
38	5103.5
39	5448.0
40	6722.0
41	7996.0
42	9636.5
43	11277.0
44	15154.5
45	19032.0
46	82016.5
47	145001.0
48	109573.5
49	74146.0
50	65155.5
51	56165.0
52	39555.0
53	22945.0
54	17079.5
55	11214.0
56	8952.0
57	6690.0
58	5322.0
59	3954.0
60	3270.5
61	2587.0
62	2128.5
63	1670.0
64	1325.0
65	980.0
66	733.5
67	487.0
68	385.5
69	284.0
70	229.0
71	174.0
72	151.5
73	129.0
74	90.5
75	37.0
76	22.0
77	14.5
78	7.0
79	14.0
80	21.0
81	11.5
82	2.0
83	1.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	408783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.18300173930912
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28538480213645	17.72260588136004
2	11.292182568584607	5.461577413933554
3	4.606700655498908	3.3421154989321966
4	2.4925143643279113	2.4110591683117937
5	1.5224164441207413	1.8408299758062345
6	1.1026139030509023	1.5998708361159832
7	0.8689406813951606	1.4709515806674935
8	0.6625799142186615	1.2818536974384942
9	0.5442259448086105	1.18449152728954
>10	3.2987375576596265	13.183033541022986
>50	0.15780529254673464	2.6720778505955485
>100	0.12948126567937202	6.495867000340033
>500	0.015173585821801408	2.458027853408777
>1k	0.011127296269321033	5.698377867964177
>5k	0.005057861940600469	7.756438012343958
>10k+	0.005057861940600469	25.420822294469193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	30466	7.452853959191062	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	26643	6.517638942911031	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	23451	5.736784553173688	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	12951	3.168184586932431	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAACAAT	10405	2.5453602522609793	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	7599	1.8589324898540303	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTA	6940	1.6977222634013645	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC	5973	1.4611664379389555	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	5690	1.391936553134548	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	5505	1.3466802680150594	No Hit
CCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4523	1.1064550140294485	No Hit
TCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	4119	1.0076250724712135	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAACAATCGT	2477	0.6059449634647233	No Hit
TGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCTT	2192	0.5362258215238892	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATG	2062	0.5044241076561403	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT	1705	0.4170917088039376	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	1426	0.3488403382723841	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAACAA	1243	0.3040733102893222	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1194	0.29208651044686296	No Hit
GCTGTCTCTTATACACATCTGACGCCGGACCAATCGTATGCCGTCTTCTGC	1184	0.2896402247647285	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	1169	0.28597079624152666	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	952	0.23288639693920735	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	873	0.21356074005034453	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	763	0.18665159754686472	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	760	0.18591771184222436	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCGGAACAATCG	726	0.17760034052296694	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	715	0.17490942627261896	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	690	0.16879371206728264	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	657	0.1607209693162387	No Hit
GGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	600	0.14677714092807187	No Hit
TTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	585	0.14310771240487005	No Hit
GCACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	567	0.13870439817702793	No Hit
TGTCTCTTATACACATCTGACGCCGGAACCATCGTATGCCGTCTTCTGCTT	561	0.13723662676774717	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	542	0.1325886839716916	No Hit
TTGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	537	0.13136554113062432	No Hit
GGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTG	520	0.1272068554709956	No Hit
CGTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	494	0.12084651269744584	No Hit
GCCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	467	0.11424154135568261	No Hit
GCATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	450	0.1100828556960539	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGT	448	0.10959359855962698	No Hit
GACTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	446	0.10910434142320008	No Hit
TGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	432	0.10567954146821174	No Hit
ACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	421	0.10298862721786375	No Hit
TCTGTCTCTTATACACATCTGACGCCGGACCAATCGTATGCCGTCTTCTGC	413	0.10103159867215614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.5203665514466111	0.0
2	0.0	0.0	0.0	7.8134364687376925	0.0
3	0.0	0.0	0.0	9.49085456097734	0.0
4	0.0	0.0	0.0	13.719259362546877	0.0
5	0.0	0.0	0.0	23.032024325864825	0.0
6	0.0	0.0	0.0	24.780629331454588	0.0
7	0.0	0.0	0.0	27.616119065616722	0.0
8	0.0	0.0	0.0	30.98587759275704	0.0
9	0.0	0.0	0.0	31.70655335471387	0.0
10	0.0	0.0	0.0	40.13425215823554	0.0
11	0.0	0.0	0.0	42.79581098039791	0.0
12	0.0	0.0	0.0	53.17809204394508	0.0
13	0.0	0.0	0.0	54.45847796997429	0.0
14	0.0	0.0	0.0	55.138300761039474	0.0
15	0.0	0.0	0.0	57.494073872935026	0.0
16	0.0	0.0	0.0	58.84858225513292	0.0
17	0.0	0.0	0.0	60.05778126781202	0.0
18	0.0	0.0	0.0	61.13463622508764	0.0
19	0.0	0.0	0.0	64.88210126154952	0.0
20	0.0	0.0	0.0	65.98855627557897	0.0
21	0.0	0.0	0.0	66.8599232355553	0.0
22	0.0	0.0	0.0	67.7892671661982	0.0
23	0.0	0.0	0.0	68.52633304222533	0.0
24	0.0	0.0	0.0	69.11882343443833	0.0
25	0.0	0.0	0.0	69.58851028540815	0.0
26	0.0	0.0	0.0	70.05330456501372	0.0
27	0.0	0.0	0.0	70.50415501623111	0.0
28	0.0	0.0	0.0	70.93616906769607	0.0
29	2.446285682134531E-4	0.0	0.0	71.34910209084038	0.0
30	2.446285682134531E-4	0.0	0.0	71.8187889418102	0.0
31	2.446285682134531E-4	0.0	0.0	72.26450219309511	0.0
32	2.446285682134531E-4	0.0	0.0	72.64538887380345	0.0
33	2.446285682134531E-4	0.0	0.0	72.98909201214336	0.0
34	2.446285682134531E-4	0.0	0.0	73.33303977905148	0.0
35	2.446285682134531E-4	0.0	0.0	73.70316280275843	0.0
36	2.446285682134531E-4	0.0	0.0	74.07059491221504	0.0
37	2.446285682134531E-4	0.0	0.0	74.41258565057744	0.0
38	2.446285682134531E-4	0.0	0.0	74.75384250323522	0.0
39	2.446285682134531E-4	0.0	0.0	75.09950267012083	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCCCTT	20	7.0279464E-4	45.000004	18
CCTTAAG	20	7.0279464E-4	45.000004	25
ACCGGTG	40	6.7957444E-9	45.000004	17
CGACGAA	20	7.0279464E-4	45.000004	19
GACCGTG	20	7.0279464E-4	45.000004	15
TTCACCC	20	7.0279464E-4	45.000004	22
ACCGACG	20	7.0279464E-4	45.000004	17
TTCGTTG	40	6.7957444E-9	45.000004	1
TTCGTCG	20	7.0279464E-4	45.000004	1
GCGTTGG	40	6.7957444E-9	45.000004	2
GCACCGA	65	0.0	45.000004	9
TTTCACC	20	7.0279464E-4	45.000004	21
TAGTTGG	20	7.0279464E-4	45.000004	2
CTCGGGT	105	0.0	45.000004	4
ATGAGAC	20	7.0279464E-4	45.000004	11
TCTCGTG	20	7.0279464E-4	45.000004	1
TCTAACC	20	7.0279464E-4	45.000004	24
CGGGCTA	20	7.0279464E-4	45.000004	6
AGACCGT	20	7.0279464E-4	45.000004	14
TCATGAC	20	7.0279464E-4	45.000004	36
>>END_MODULE