SRR2933820.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997064
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	59623	5.979856859740197	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	47007	4.71454189500373	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	38057	3.8169064372999126	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTATAGT	18188	1.8241557211974357	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	12779	1.281662962457776	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTA	11952	1.1987194402766523	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT	11610	1.1644187334012661	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC	8862	0.8888095448236022	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT	7670	0.7692585430824902	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	4333	0.4345759148861056	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGT	4209	0.4221394012821645	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3851	0.38623398297401174	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATG	3764	0.3775083645583433	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2773	0.27811655019136183	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2355	0.23619346401033436	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTT	2245	0.22516107291006396	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTAT	2201	0.22074811646995582	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCCTATAG	2066	0.20720836375598758	No Hit
TGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCTT	1852	0.18574534834273426	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG	1494	0.14983993003458154	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTCTAGTCGTATGCCGTCTTCTGC	1225	0.12286071907119303	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	1182	0.11854805709563278	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	1040	0.10430624312982918	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCCTATAGTCG	1015	0.10179888151613137	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCCG	30	2.1652104E-6	45.000004	1
CCGATCA	30	2.1652104E-6	45.000004	40
CGTAACC	70	0.0	45.000004	35
ATCGAAT	35	1.2117198E-7	45.000004	43
ACGTTGT	20	7.0328376E-4	45.0	15
GCGAAAT	20	7.0328376E-4	45.0	19
CACGCGA	20	7.0328376E-4	45.0	41
GTTCGCG	75	0.0	45.0	1
GCGATCG	25	3.8904327E-5	45.0	9
CGCAATT	25	3.8904327E-5	45.0	10
CGATCGT	20	7.0328376E-4	45.0	10
TACCGCG	20	7.0328376E-4	45.0	29
TACGGCT	7280	0.0	44.041893	7
ACGGCTG	7280	0.0	44.010986	8
TGATACC	5280	0.0	43.551136	4
CGTTTTT	2475	0.0	43.363636	1
GGCGACT	1600	0.0	43.3125	10
GATACCT	5315	0.0	43.306683	5
GCGACTG	1615	0.0	42.63158	11
CGGCTGT	7670	0.0	42.56519	9

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content