##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933820.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	997064
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.3513726300418	30.0	16.0	31.0	16.0	34.0
2	26.19673461282325	30.0	16.0	33.0	16.0	34.0
3	27.265767292771578	31.0	25.0	33.0	16.0	34.0
4	31.399128842280938	35.0	28.0	35.0	19.0	37.0
5	33.32762992144937	35.0	32.0	35.0	28.0	37.0
6	31.75966838638242	35.0	30.0	37.0	17.0	37.0
7	33.126831376922645	35.0	32.0	35.0	28.0	37.0
8	32.30233264865645	35.0	32.0	37.0	19.0	37.0
9	34.24693399821877	35.0	32.0	38.0	28.0	39.0
10	33.14971255606461	34.0	31.0	37.0	27.0	39.0
11	33.85962084680622	35.0	32.0	37.0	27.0	39.0
12	34.04723768985742	35.0	32.0	39.0	27.0	39.0
13	34.28074927988575	35.0	33.0	37.0	27.0	39.0
14	34.32245974180193	36.0	32.0	39.0	25.0	40.0
15	34.77836126868486	37.0	32.0	39.0	27.0	40.0
16	34.58739258462847	36.0	32.0	39.0	27.0	40.0
17	33.98508821901102	36.0	32.0	38.0	26.0	40.0
18	34.34530882671523	36.0	32.0	39.0	27.0	40.0
19	34.18958562339027	36.0	32.0	39.0	27.0	40.0
20	33.81396179182079	36.0	31.0	39.0	25.0	40.0
21	33.71045690146269	36.0	32.0	39.0	25.0	40.0
22	34.83163768825271	37.0	33.0	39.0	27.0	40.0
23	35.247169690210455	37.0	33.0	39.0	28.0	40.0
24	35.53143128224467	37.0	34.0	39.0	29.0	40.0
25	33.60646558295154	36.0	32.0	39.0	24.0	40.0
26	34.477204071152904	36.0	32.0	39.0	27.0	40.0
27	34.70955124244783	37.0	33.0	39.0	27.0	40.0
28	34.88998599889275	37.0	33.0	39.0	27.0	40.0
29	35.30274586185039	37.0	34.0	40.0	27.0	40.0
30	33.321011489733856	36.0	31.0	39.0	23.0	40.0
31	34.2742160984651	36.0	32.0	39.0	25.0	40.0
32	34.427275480811666	37.0	33.0	39.0	25.0	40.0
33	34.84748120481734	37.0	33.0	39.0	25.0	40.0
34	35.15873504609534	38.0	34.0	40.0	27.0	40.0
35	35.1850212223087	38.0	34.0	40.0	27.0	41.0
36	35.103508902136674	38.0	34.0	40.0	26.0	41.0
37	35.47898530084328	38.0	35.0	40.0	29.0	41.0
38	35.149591199762504	38.0	34.0	40.0	27.0	41.0
39	34.90598998660066	37.0	34.0	40.0	27.0	41.0
40	34.44994503863343	37.0	33.0	40.0	25.0	40.0
41	34.15819947365465	37.0	33.0	39.0	24.0	40.0
42	34.83838249099356	37.0	33.0	40.0	27.0	40.0
43	34.84649932200942	37.0	34.0	40.0	27.0	40.0
44	35.528056373512634	38.0	35.0	40.0	29.0	41.0
45	35.79214473694768	38.0	35.0	40.0	30.0	41.0
46	35.47414107820561	38.0	35.0	40.0	29.0	41.0
47	34.93571726589266	37.0	34.0	40.0	26.0	41.0
48	35.33278906870572	37.0	34.0	40.0	28.0	41.0
49	35.536959513130554	38.0	35.0	40.0	29.0	41.0
50	35.08311101393692	38.0	34.0	40.0	26.0	41.0
51	34.59586044627025	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	10.0
11	18.0
12	24.0
13	26.0
14	32.0
15	92.0
16	235.0
17	817.0
18	1910.0
19	3622.0
20	5611.0
21	6868.0
22	7196.0
23	7095.0
24	8082.0
25	10432.0
26	13739.0
27	17474.0
28	22996.0
29	30189.0
30	40581.0
31	55491.0
32	73777.0
33	92780.0
34	109306.0
35	129124.0
36	141931.0
37	133148.0
38	76507.0
39	7945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.99175980679274	2.929601309444529	41.13346786164178	11.945171022120947
2	15.635505845161394	30.242491956383944	38.007489990612434	16.114512207842225
3	13.97703657939711	30.140492485938715	40.39098794059358	15.49148299407059
4	14.068003658742068	2.9214774578161484	64.30560124525607	18.704917638185712
5	35.064850400776685	3.602276283167379	40.412450956006836	20.920422360049105
6	15.818944420819525	25.846685869713475	43.47393948633187	14.860430223135124
7	62.0602087729574	1.1177818073864867	32.648756749817466	4.1732526698386465
8	59.54061123458474	21.175872361252637	12.028916900018455	7.254599504144167
9	56.857132541140786	2.642558551908403	11.681496874824484	28.818812032126324
10	43.616558214919	15.989344716086428	22.011124662007656	18.382972406986912
11	34.23180457824172	20.02068071858978	26.422075212824854	19.325439490343648
12	23.034930556112748	15.160210377668836	30.71257211172001	31.092286954498405
13	24.78366483997015	19.19034284659761	40.96376962762671	15.062222685805526
14	21.22622018245569	27.91515890654963	27.81045148556161	23.04816942543307
15	13.219412194202176	21.420390265820448	43.11057264127478	22.249624898702592
16	15.86146927378784	22.914577198655252	25.50397968435326	35.71997384320365
17	17.232594898622356	27.49913746760489	34.1128553432879	21.15541229048486
18	16.72540579140356	22.361352932208966	32.241160045894745	28.672081230492726
19	16.894201375237696	27.343380164161978	28.18264424349891	27.57977421710141
20	18.96217293975111	25.621524796803413	40.037750836455835	15.378551426989642
21	20.213045501592678	33.76944709667584	28.66285413975432	17.354653261977166
22	16.53765455376987	19.665337430696525	36.907861481309126	26.889146534224484
23	18.768504328709092	30.67797052145098	29.913826996060433	20.639698153779495
24	20.85282389094381	24.46703521539239	25.835152006290468	28.84498888737333
25	15.797882583264464	36.86333073904985	22.89943273450852	24.43935394317717
26	18.138354207954556	20.732169650092672	31.97718501520464	29.152291126748132
27	21.27476270329688	26.257792879895376	25.335083806054577	27.13236061075317
28	14.496762494684393	25.319939341907844	34.34423467299993	25.83906349040784
29	24.264039219147417	18.570723644620607	29.068545248850626	28.096691887381354
30	16.735334943393802	29.668707324705334	29.08469265764284	24.51126507425802
31	22.23899368546051	19.959400800751006	26.247562844511485	31.554042669277
32	24.116606356261986	30.820388661109018	26.093009074643152	18.969995907985844
33	19.985477361533462	23.33451012171736	23.261796634920128	33.41821588182905
34	27.35621785562411	22.07942519236478	29.138550785105068	21.425806166906035
35	19.24169361244614	25.93394205387016	24.12222284627667	30.702141487407026
36	28.69825808573973	23.380244397551213	21.675539383630337	26.245958133078716
37	15.519063971821268	20.793449567931447	35.45479527893897	28.232691181308322
38	19.591821588182906	27.216006194186132	21.535829194515095	31.65634302311587
39	21.47655516596728	22.665846926576428	36.8008472876365	19.05675061981979
40	17.50048141342983	32.24196240161113	27.17608899729606	23.08146718766298
41	22.988093041168874	23.457671724182198	28.76605714377412	24.78817809087481
42	18.788061749295935	25.303591344186533	32.73270321664407	23.175643689873468
43	29.901390482456492	20.59095504400921	23.568196224114	25.939458249420298
44	20.892941676762977	18.57764396267441	32.10887164715605	28.42054271340656
45	17.949098553352645	17.88771834104932	27.993789766755196	36.169393338842845
46	29.03765455376987	23.457270546324008	26.922143413060745	20.582931486845375
47	14.292863848258486	22.32073367406706	41.66863912446944	21.71776335320501
48	20.279440437123395	25.294364253448126	27.722292651223995	26.703902658204488
49	19.138490608426338	19.79531905675062	37.63319104891963	23.432999285903414
50	25.583513194739755	18.191209390771306	29.212768688870522	27.012508725618417
51	20.0485625797341	18.738315694880168	26.95173028010238	34.26139144528335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	275.5
2	483.0
3	3463.0
4	6443.0
5	4568.0
6	2693.0
7	2577.5
8	2462.0
9	2553.0
10	2644.0
11	2684.0
12	2724.0
13	2660.0
14	2596.0
15	2412.5
16	2229.0
17	2318.5
18	2408.0
19	2392.5
20	2377.0
21	2464.5
22	2552.0
23	2945.0
24	3338.0
25	3747.5
26	5106.5
27	6056.0
28	6891.5
29	7727.0
30	9162.0
31	10597.0
32	11954.5
33	13312.0
34	15688.5
35	18065.0
36	19618.5
37	21172.0
38	24100.0
39	27028.0
40	30280.0
41	33532.0
42	37303.5
43	41075.0
44	47878.0
45	54681.0
46	159112.5
47	263544.0
48	194899.5
49	126255.0
50	113477.5
51	100700.0
52	81645.0
53	62590.0
54	55188.0
55	47786.0
56	42587.0
57	37388.0
58	32730.5
59	28073.0
60	24036.0
61	19999.0
62	16869.5
63	13740.0
64	11748.0
65	9756.0
66	7894.5
67	6033.0
68	4968.5
69	3904.0
70	3327.0
71	2750.0
72	2334.0
73	1918.0
74	1461.0
75	783.5
76	563.0
77	415.5
78	268.0
79	224.5
80	181.0
81	152.5
82	124.0
83	87.0
84	50.0
85	32.0
86	14.0
87	8.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	997064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.903673213711535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.7007411298705	23.3943096591329
2	10.441434907499508	6.4535738452721025
3	3.759267837231648	3.4852555429386896
4	1.8877163406631128	2.3334947564814477
5	1.1211296631304812	1.7323512369791443
6	0.8105925486481586	1.5030172339735355
7	0.6353438142696419	1.3744120330179437
8	0.516975870497667	1.2781162688987167
9	0.48473082673968154	1.3481966759557809
>10	4.490192448758925	25.4655395851811
>50	0.0849970793303479	1.793501243616814
>100	0.05337024829750102	3.1638757829940136
>500	0.005600581611465477	1.0838789286186141
>1k	0.004941689657175421	3.624977386198173
>5k	6.58891954290056E-4	1.6831379342403652
>10k+	0.0023061218400151962	20.282361886500638
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	59623	5.979856859740197	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	47007	4.71454189500373	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	38057	3.8169064372999126	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCTATAGT	18188	1.8241557211974357	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	12779	1.281662962457776	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTA	11952	1.1987194402766523	No Hit
GAACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCT	11610	1.1644187334012661	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTC	8862	0.8888095448236022	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCT	7670	0.7692585430824902	No Hit
CTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCT	4333	0.4345759148861056	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGT	4209	0.4221394012821645	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3851	0.38623398297401174	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATG	3764	0.3775083645583433	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2773	0.27811655019136183	No Hit
TCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGC	2355	0.23619346401033436	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTT	2245	0.22516107291006396	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTAT	2201	0.22074811646995582	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCCTATAG	2066	0.20720836375598758	No Hit
TGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTGCTT	1852	0.18574534834273426	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTCTG	1494	0.14983993003458154	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTCTAGTCGTATGCCGTCTTCTGC	1225	0.12286071907119303	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	1182	0.11854805709563278	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	1040	0.10430624312982918	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCCTATAGTCG	1015	0.10179888151613137	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.48392079144367867	0.0
2	0.0	0.0	0.0	2.580977750675985	0.0
3	0.0	0.0	0.0	3.1174528415427694	0.0
4	0.0	0.0	0.0	5.013519693821059	0.0
5	0.0	0.0	0.0	10.06434892845394	0.0
6	0.0	0.0	0.0	10.883955292739483	0.0
7	0.0	0.0	0.0	12.269523320468897	0.0
8	0.0	0.0	0.0	14.037915319377692	0.0
9	0.0	0.0	0.0	14.41331750018053	0.0
10	0.0	0.0	0.0	20.27302159139233	0.0
11	0.0	0.0	0.0	21.85426411945472	0.0
12	0.0	0.0	0.0	29.642630763922877	0.0
13	0.0	0.0	0.0	30.486809272022658	0.0
14	0.0	0.0	0.0	30.857999085314482	0.0
15	0.0	0.0	0.0	32.403035311675076	0.0
16	0.0	0.0	0.0	33.34329591681176	0.0
17	0.0	0.0	0.0	34.221474248393285	0.0
18	0.0	0.0	0.0	35.01259698474722	0.0
19	0.0	0.0	0.0	37.69507273354569	0.0
20	0.0	0.0	0.0	38.551286577391224	0.0
21	0.0	0.0	0.0	39.50267986809272	0.0
22	0.0	0.0	0.0	40.58575979074563	0.0
23	0.0	0.0	0.0	41.65901085587284	0.0
24	0.0	0.0	0.0	42.37541421613858	0.0
25	0.0	0.0	0.0	43.02943441945552	0.0
26	0.0	0.0	0.0	43.648652443574335	0.0
27	0.0	0.0	0.0	44.335769820192084	0.0
28	0.0	0.0	0.0	44.93081687835485	0.0
29	0.0	0.0	0.0	45.58784591560823	0.0
30	2.0058892909582534E-4	0.0	0.0	46.269547391140385	0.0
31	2.0058892909582534E-4	0.0	0.0	46.888564826330104	0.0
32	2.0058892909582534E-4	0.0	0.0	47.47438479375446	0.0
33	3.00883393643738E-4	0.0	0.0	48.05147914276315	0.0
34	3.00883393643738E-4	0.0	0.0	48.64813091235869	0.0
35	3.00883393643738E-4	0.0	0.0	49.2765760272159	0.0
36	3.00883393643738E-4	0.0	0.0	49.84695064709989	0.0
37	3.00883393643738E-4	0.0	0.0	50.43868798793257	0.0
38	3.00883393643738E-4	0.0	0.0	51.067835164041625	0.0
39	3.00883393643738E-4	0.0	0.0	52.00408399059639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTCCG	30	2.1652104E-6	45.000004	1
CCGATCA	30	2.1652104E-6	45.000004	40
CGTAACC	70	0.0	45.000004	35
ATCGAAT	35	1.2117198E-7	45.000004	43
ACGTTGT	20	7.0328376E-4	45.0	15
GCGAAAT	20	7.0328376E-4	45.0	19
CACGCGA	20	7.0328376E-4	45.0	41
GTTCGCG	75	0.0	45.0	1
GCGATCG	25	3.8904327E-5	45.0	9
CGCAATT	25	3.8904327E-5	45.0	10
CGATCGT	20	7.0328376E-4	45.0	10
TACCGCG	20	7.0328376E-4	45.0	29
TACGGCT	7280	0.0	44.041893	7
ACGGCTG	7280	0.0	44.010986	8
TGATACC	5280	0.0	43.551136	4
CGTTTTT	2475	0.0	43.363636	1
GGCGACT	1600	0.0	43.3125	10
GATACCT	5315	0.0	43.306683	5
GCGACTG	1615	0.0	42.63158	11
CGGCTGT	7670	0.0	42.56519	9
>>END_MODULE