##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933819.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359742
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.557035319756938	28.0	16.0	31.0	16.0	33.0
2	25.39550566795092	28.0	16.0	31.0	16.0	34.0
3	26.598086962323	30.0	16.0	33.0	16.0	34.0
4	31.060001334289574	33.0	28.0	35.0	19.0	37.0
5	33.15587559973537	35.0	32.0	35.0	28.0	37.0
6	31.34754073752856	35.0	28.0	36.0	17.0	37.0
7	32.98503927814934	35.0	32.0	35.0	28.0	37.0
8	31.841466940196028	35.0	30.0	36.0	17.0	37.0
9	34.168237236686295	35.0	32.0	37.0	28.0	39.0
10	33.30779836660718	35.0	32.0	37.0	27.0	39.0
11	33.835034552540435	35.0	32.0	38.0	27.0	39.0
12	34.105030827648704	35.0	32.0	39.0	27.0	39.0
13	34.235749509370606	35.0	32.0	37.0	27.0	39.0
14	34.273482106620854	36.0	32.0	39.0	25.0	40.0
15	34.79603160042475	36.0	32.0	39.0	27.0	40.0
16	34.60738529279317	36.0	32.0	39.0	27.0	40.0
17	34.00156501047973	36.0	32.0	38.0	27.0	40.0
18	34.38037538013354	36.0	32.0	38.0	27.0	40.0
19	34.067170360980924	36.0	32.0	39.0	27.0	40.0
20	33.65286510888359	35.0	31.0	39.0	25.0	40.0
21	33.83341672643172	36.0	32.0	39.0	25.0	40.0
22	34.9615252041741	37.0	33.0	39.0	27.0	40.0
23	35.273634993967896	37.0	33.0	39.0	28.0	40.0
24	35.70894418777902	37.0	34.0	39.0	30.0	40.0
25	33.77496928354209	36.0	32.0	39.0	24.0	40.0
26	34.71495683017273	37.0	33.0	39.0	27.0	40.0
27	34.610634843860325	37.0	33.0	39.0	27.0	40.0
28	34.777401582245055	37.0	33.0	39.0	27.0	40.0
29	35.17876700524265	37.0	33.0	40.0	27.0	40.0
30	33.48543400548171	36.0	31.0	39.0	23.0	40.0
31	34.28796748781071	36.0	32.0	39.0	25.0	40.0
32	34.43506457405585	37.0	33.0	39.0	26.0	40.0
33	34.91099732586131	37.0	34.0	39.0	26.0	40.0
34	34.94583340282758	37.0	34.0	40.0	25.0	40.0
35	35.367605117000515	38.0	34.0	40.0	27.0	41.0
36	35.10648464733059	38.0	34.0	40.0	25.0	41.0
37	35.241411900751096	38.0	34.0	40.0	27.0	41.0
38	34.7786608180307	37.0	34.0	40.0	25.0	41.0
39	34.77420484680688	37.0	33.0	40.0	26.0	40.0
40	34.57040601319835	37.0	33.0	40.0	25.0	40.0
41	33.96691239832992	37.0	32.0	39.0	24.0	40.0
42	34.78608836332705	37.0	33.0	40.0	26.0	40.0
43	35.009946016867644	38.0	34.0	40.0	27.0	40.0
44	35.65595343329386	38.0	35.0	40.0	30.0	41.0
45	35.83011991927548	38.0	35.0	40.0	30.0	41.0
46	35.65565043836972	38.0	35.0	40.0	30.0	41.0
47	34.94882165551979	37.0	34.0	40.0	26.0	41.0
48	35.4742760089175	38.0	35.0	40.0	29.0	41.0
49	35.68429596766572	38.0	35.0	40.0	30.0	41.0
50	35.34743788604055	38.0	35.0	40.0	28.0	41.0
51	35.05832235324205	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	8.0
12	8.0
13	7.0
14	12.0
15	28.0
16	66.0
17	246.0
18	772.0
19	1661.0
20	2382.0
21	2717.0
22	2979.0
23	2789.0
24	2837.0
25	3274.0
26	4409.0
27	5834.0
28	7961.0
29	10760.0
30	14860.0
31	20095.0
32	26874.0
33	34210.0
34	40763.0
35	48020.0
36	50760.0
37	46281.0
38	26342.0
39	2778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.0592090998549	2.8781738023361187	34.90390335295851	12.158713744850475
2	13.894680076276888	35.9843999310617	33.92486837789305	16.19605161476836
3	13.935264717491979	35.95215459968533	35.706978890427024	14.405601792395661
4	14.370020737083799	2.9471120970028517	64.8103362965681	17.87253086934525
5	39.05993739958081	3.7335090147939356	36.2387488811426	20.967804704482656
6	15.663447693068921	30.643349956357614	38.60739085233306	15.085811498240407
7	56.491874732447144	1.3317877812432244	37.370949180245844	4.80538830606379
8	52.229931450873124	25.86742721172396	12.913143308259809	8.989498029143109
9	49.42681143708547	3.317655430836544	12.408337086022762	34.84719604605523
10	41.80273640553508	20.316782583073426	20.393782210584252	17.486698800807247
11	36.098648475852144	18.274207626576825	26.632697877923622	18.994446019647416
12	24.935926302739187	14.744455748842226	28.989942792334507	31.32967515608408
13	25.05656831840597	19.53594520517482	40.6399586370232	14.76752783939601
14	19.9934953383258	31.03112786385799	23.222754084871937	25.75262271294428
15	11.266685569102302	21.369203484719606	44.9702842592747	22.39382668690339
16	13.19167625687298	23.653896403533643	23.400381384436624	39.754045955156755
17	14.415330987207497	28.835109606328977	35.043725781254345	21.705833625209177
18	13.993361909368382	23.127963929705178	31.83336947034263	31.04530469058381
19	14.821733353347676	27.12193738846173	27.179478626348885	30.876850631841705
20	19.007232961400113	24.879496972830527	41.74324932868556	14.370020737083799
21	20.692329502810345	36.44639769612667	25.66589389062161	17.195378910441374
22	15.398257640197697	19.782232822411615	36.179817758282326	28.63969177910836
23	18.70173624430842	33.112063645612686	27.882204468758164	20.303995641320725
24	21.179067220396842	23.762029454442352	24.022493898404967	31.03640942675584
25	14.700813360686269	40.84176993512017	19.6763235874599	24.78109311673366
26	15.342940218267536	23.903241767711304	30.9627455231805	29.79107249084066
27	25.140239393787773	28.81453930872681	22.056918569419196	23.988302728066223
28	11.88546235913516	28.739207543183724	37.204440960466115	22.170889137215006
29	22.724897287500486	26.825613912192626	25.368736483368636	25.08075231693825
30	23.865436896442453	30.81180401509971	28.977155850581802	16.345603237876034
31	22.32989197813989	22.590078445107885	27.350990432031846	27.729039144720385
32	27.357661879902818	35.62275186105598	18.09602437302289	18.923561886018312
33	25.133289968922174	18.457394466033993	21.347799256133563	35.06151630891027
34	22.94894674516737	27.343485053176998	28.47957703020498	21.22799117145065
35	25.02460096402422	21.000328012853657	20.294822400498134	33.68024862262399
36	18.315904175770413	29.807751110518094	26.878429541171172	24.99791517254032
37	23.06875482985028	25.589172240105405	31.606540242729515	19.735532687314798
38	25.40153776873426	20.642849597767288	26.88815873598301	27.067453897515442
39	20.089675378465678	31.265184493331333	29.921443701319276	18.72369642688371
40	26.870924162316328	19.1464994357067	26.026152075654217	27.956424326322754
41	17.374674071973804	21.876789476902893	29.567023033173772	31.18151341794953
42	17.363554992188845	18.12437802647453	32.28702792556888	32.225039055767745
43	22.416064846473304	21.453430514090655	21.00060598984828	35.12989864958776
44	25.061571904309197	17.423320046032988	31.88813093828355	25.62697711137426
45	18.289774338275762	15.864147083187397	29.813588627405196	36.032489951131645
46	29.498640692496288	27.2667634026608	22.891961461269467	20.34263444357345
47	15.748786630418465	19.39667873086823	44.52607702186567	20.32845761684763
48	21.636339376553195	23.673632770151944	27.73876833953222	26.951259513762643
49	18.736761345631038	15.948930066547693	39.26675228358101	26.04755630424026
50	24.151197246915846	16.666944643661292	28.56297012859216	30.618887980830706
51	19.76249645579332	17.107816157134835	26.33859821761151	36.791089169460335
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	143.5
2	239.0
3	847.0
4	1455.0
5	1058.0
6	661.0
7	697.5
8	734.0
9	780.0
10	826.0
11	870.0
12	914.0
13	915.0
14	916.0
15	832.0
16	748.0
17	704.0
18	660.0
19	653.5
20	647.0
21	756.0
22	865.0
23	970.5
24	1076.0
25	1156.0
26	1710.0
27	2184.0
28	2487.5
29	2791.0
30	3299.0
31	3807.0
32	4384.0
33	4961.0
34	5760.0
35	6559.0
36	7155.0
37	7751.0
38	8077.0
39	8403.0
40	9095.5
41	9788.0
42	11340.0
43	12892.0
44	15089.5
45	17287.0
46	62496.0
47	107705.0
48	77491.0
49	47277.0
50	42357.0
51	37437.0
52	28526.0
53	19615.0
54	16993.5
55	14372.0
56	13104.0
57	11836.0
58	10684.5
59	9533.0
60	8925.5
61	8318.0
62	6744.5
63	5171.0
64	4429.0
65	3687.0
66	3217.5
67	2748.0
68	2312.0
69	1876.0
70	1557.0
71	1238.0
72	935.5
73	633.0
74	543.5
75	308.5
76	163.0
77	155.5
78	148.0
79	106.0
80	64.0
81	39.0
82	14.0
83	7.5
84	1.0
85	1.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.7033040345581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94456762749445	20.012675750954852
2	10.027794260016865	5.355504778424538
3	3.6934095333270878	2.958787130777057
4	1.835253947930004	1.9602937660879185
5	1.1305081040567129	1.5094150808079123
6	0.7880245255717603	1.262571509581867
7	0.6672704371089805	1.247282774877551
8	0.5673360190708181	1.2119796965603127
9	0.5236147111791221	1.258401854662508
>10	5.627556915774024	27.404362014999638
>50	0.10513933564431675	1.9038644361792616
>100	0.06870491240123669	3.8041151714284127
>500	0.005204917606154294	1.1169115643989302
>1k	0.010409835212308588	7.112319384447743
>5k	0.002081967042461718	4.044287294783484
>10k+	0.0031229505636925763	17.837227791028017
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	25867	7.190430919937066	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG	21896	6.08658427428546	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTC	16405	4.5602125968054885	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTAGTCCT	7606	2.1142930211095727	No Hit
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	6943	1.9299942736739109	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTA	4587	1.2750804743399438	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCT	4359	1.2117017195656887	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTC	3813	1.0599262805010259	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCT	3482	0.9679158952805066	No Hit
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	1985	0.5517843343284909	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1793	0.4984127513606974	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGT	1633	0.45393643222086943	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATG	1440	0.4002868722584519	No Hit
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1435	0.3988969872853323	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTAT	1059	0.2943776373067365	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGTAGTCC	901	0.2504572721561564	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTT	860	0.23906021537657543	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	842	0.23405662947334477	No Hit
TGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCTT	813	0.22599529662925094	TruSeq Adapter, Index 22 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	602	0.16734215076360281	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	498	0.1384325433227146	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	450	0.1250896475807662	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	441	0.1225878546291509	No Hit
GTGCTTGGGAGGCAGAGGCAGGCAGATTTCTGAGCTCGAGGTCAGCCTGGT	404	0.11230270582806567	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCG	399	0.11091282085494605	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.779769946239249E-4	0.0	0.0	0.6237803759360875	0.0
2	2.779769946239249E-4	0.0	0.0	3.6634588121487064	0.0
3	2.779769946239249E-4	0.0	0.0	4.408993111730073	0.0
4	2.779769946239249E-4	0.0	0.0	6.620300103963396	0.0
5	2.779769946239249E-4	0.0	0.0	12.797226901501631	0.0
6	2.779769946239249E-4	0.0	0.0	13.672298480577748	0.0
7	2.779769946239249E-4	0.0	0.0	15.312918702848153	0.0
8	2.779769946239249E-4	0.0	0.0	17.29294883555437	0.0
9	2.779769946239249E-4	0.0	0.0	17.678502927097753	0.0
10	2.779769946239249E-4	0.0	0.0	25.001250896475806	0.0
11	2.779769946239249E-4	0.0	0.0	26.452290808412695	0.0
12	2.779769946239249E-4	0.0	0.0	35.48543122571176	0.0
13	2.779769946239249E-4	0.0	0.0	36.33326105931473	0.0
14	2.779769946239249E-4	0.0	0.0	36.76801707890655	0.0
15	2.779769946239249E-4	0.0	0.0	38.42170221992428	0.0
16	2.779769946239249E-4	0.0	0.0	39.349867404973565	0.0
17	2.779769946239249E-4	0.0	0.0	40.03674855868928	0.0
18	2.779769946239249E-4	0.0	0.0	40.64607413090493	0.0
19	2.779769946239249E-4	0.0	0.0	43.5039556126335	0.0
20	2.779769946239249E-4	0.0	0.0	44.305363288134274	0.0
21	2.779769946239249E-4	0.0	0.0	44.93108950303273	0.0
22	2.779769946239249E-4	0.0	0.0	45.6999738701625	0.0
23	2.779769946239249E-4	0.0	0.0	46.282613650894255	0.0
24	2.779769946239249E-4	0.0	0.0	46.75128286383019	0.0
25	5.559539892478499E-4	0.0	0.0	47.17769957358329	0.0
26	5.559539892478499E-4	0.0	0.0	47.579654307809484	0.0
27	5.559539892478499E-4	0.0	0.0	48.0608324855035	0.0
28	5.559539892478499E-4	0.0	0.0	48.45139016295011	0.0
29	5.559539892478499E-4	0.0	0.0	48.866687792918256	0.0
30	5.559539892478499E-4	0.0	0.0	49.322292087106874	0.0
31	5.559539892478499E-4	0.0	0.0	49.71396167253198	0.0
32	5.559539892478499E-4	0.0	0.0	50.10702114293021	0.0
33	5.559539892478499E-4	0.0	0.0	50.46116383408109	0.0
34	5.559539892478499E-4	0.0	0.0	50.84421613267286	0.0
35	5.559539892478499E-4	0.0	0.0	51.25312029176465	0.0
36	5.559539892478499E-4	0.0	0.0	51.631446981447816	0.0
37	5.559539892478499E-4	0.0	0.0	52.01338737206109	0.0
38	5.559539892478499E-4	0.0	0.0	52.394771808685114	0.0
39	8.339309838717747E-4	0.0	0.0	52.97824552040073	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTG	60	0.0	45.000004	1
TACGGAG	30	2.1613287E-6	45.000004	29
GCGATAC	30	2.1613287E-6	45.000004	33
ACACGCG	35	1.2088094E-7	45.0	36
CATATAA	20	7.026817E-4	45.0	19
GGGTCTA	20	7.026817E-4	45.0	8
GCGAACC	25	3.8854447E-5	45.0	33
CGTTGAT	20	7.026817E-4	45.0	25
TGGCGAA	20	7.026817E-4	45.0	34
ATTAAGT	20	7.026817E-4	45.0	28
TTCGTAC	25	3.8854447E-5	45.0	33
CATTCTA	25	3.8854447E-5	45.0	45
CCTAAGG	20	7.026817E-4	45.0	32
TCGATGA	20	7.026817E-4	45.0	19
ATACTAT	25	3.8854447E-5	45.0	45
AGAGTTA	25	3.8854447E-5	45.0	26
TACGCAG	20	7.026817E-4	45.0	41
TCTACGC	20	7.026817E-4	45.0	39
ACGGCCT	185	0.0	45.0	8
CTACGCA	20	7.026817E-4	45.0	40
>>END_MODULE