SRR2933817.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	802699
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	49182	6.127078768006438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	41535	5.174417807920529	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	31420	3.9142941501110626	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	15976	1.990285275053289	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	11824	1.4730303638100957	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	8522	1.061668196920639	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	7967	0.992526463842611	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	6990	0.8708120976854339	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6783	0.8450240999428179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	6122	0.7626769187453828	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGT	3328	0.41460123906968865	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	3159	0.39354726989818095	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	3097	0.38582332854532025	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2772	0.34533492629242096	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATG	2734	0.3406008977213127	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2031	0.2530213691558106	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	2013	0.2507789345694962	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT	1931	0.24056339923184156	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAAGCTGA	1798	0.22399429923296277	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGCTGATCGTATGCCGTCTTC	1195	0.14887274059142966	No Hit
TGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCTT	1159	0.14438787141880083	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCAAGCTGATCG	1066	0.13280195938950964	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	1043	0.12993662630699676	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	870	0.1083843383385304	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	817	0.10178161427882682	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAG	20	7.032012E-4	45.000004	26
TCTACGG	20	7.032012E-4	45.000004	2
ACGGACG	40	6.8102963E-9	45.000004	43
CGGTGTA	20	7.032012E-4	45.000004	19
AGACACG	20	7.032012E-4	45.000004	24
GGACGCA	20	7.032012E-4	45.000004	8
CTCGAAT	20	7.032012E-4	45.000004	43
ACCAACG	20	7.032012E-4	45.000004	36
CCAACGC	20	7.032012E-4	45.000004	37
CCCTACG	25	3.889751E-5	45.0	31
GCGCGAC	45	3.8380676E-10	45.0	9
TCGGGTC	70	0.0	45.0	5
TATGCGG	35	1.2113196E-7	45.0	2
CGGTGAT	25	3.889751E-5	45.0	31
TCGTAAC	25	3.889751E-5	45.0	34
CGAGCGT	25	3.889751E-5	45.0	28
CGATCGA	140	0.0	45.0	41
TACCGCG	35	1.2113196E-7	45.0	29
CGGGTCC	60	0.0	44.999996	6
CGAATAC	60	0.0	44.999996	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content