##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933817.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	802699
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.15194985916265	28.0	16.0	31.0	16.0	34.0
2	25.906349702690548	30.0	16.0	33.0	16.0	34.0
3	26.98249032327186	31.0	19.0	33.0	16.0	34.0
4	31.250945871366476	33.0	28.0	35.0	19.0	37.0
5	33.27074283137266	35.0	32.0	35.0	28.0	37.0
6	31.562458655112316	35.0	28.0	37.0	17.0	37.0
7	33.143960563050406	35.0	32.0	35.0	28.0	37.0
8	32.084788943302534	35.0	31.0	37.0	17.0	37.0
9	34.18965265934055	35.0	32.0	38.0	28.0	39.0
10	33.436583327000534	35.0	32.0	37.0	27.0	39.0
11	34.1828929648598	35.0	32.0	39.0	27.0	39.0
12	34.36673896441879	35.0	33.0	39.0	27.0	39.0
13	34.57649878721663	35.0	33.0	38.0	27.0	39.0
14	34.57697468166772	37.0	33.0	39.0	27.0	40.0
15	35.03766667206512	37.0	33.0	39.0	27.0	40.0
16	34.966935302024794	36.0	33.0	39.0	27.0	40.0
17	34.1521217791476	36.0	32.0	38.0	27.0	40.0
18	34.552278002090446	36.0	32.0	39.0	27.0	40.0
19	34.30635144680634	36.0	32.0	39.0	27.0	40.0
20	33.698245544095606	35.0	31.0	39.0	25.0	40.0
21	33.58165140357718	36.0	32.0	39.0	25.0	40.0
22	34.650450542482304	37.0	32.0	39.0	27.0	40.0
23	35.00234209834571	37.0	33.0	39.0	27.0	40.0
24	35.34146049764607	37.0	34.0	39.0	27.0	40.0
25	33.37342640267398	36.0	31.0	39.0	24.0	40.0
26	34.172560324604866	36.0	32.0	39.0	25.0	40.0
27	34.37446664316263	37.0	33.0	39.0	26.0	40.0
28	34.378096895598475	37.0	33.0	39.0	25.0	40.0
29	34.93708476028997	37.0	33.0	40.0	26.0	40.0
30	32.772406593256	36.0	30.0	39.0	19.0	40.0
31	33.845567267431505	36.0	31.0	39.0	25.0	40.0
32	34.00230223284195	36.0	33.0	39.0	25.0	40.0
33	34.356864777457055	37.0	33.0	39.0	24.0	40.0
34	34.65644282601573	38.0	34.0	40.0	24.0	40.0
35	34.50141086509389	38.0	34.0	40.0	23.0	41.0
36	34.5558409814887	38.0	34.0	40.0	23.0	41.0
37	34.47331814291534	38.0	34.0	40.0	23.0	41.0
38	34.272199168056765	37.0	33.0	40.0	23.0	41.0
39	34.09957655360228	37.0	33.0	40.0	23.0	41.0
40	34.192054555941894	37.0	33.0	40.0	23.0	41.0
41	33.486346687861825	37.0	32.0	39.0	21.0	40.0
42	34.29945346885943	37.0	33.0	40.0	23.0	40.0
43	34.491973952876485	38.0	34.0	40.0	23.0	40.0
44	34.99832066565425	38.0	35.0	40.0	24.0	41.0
45	35.15355319989187	38.0	35.0	40.0	25.0	41.0
46	34.82290248274882	38.0	35.0	40.0	24.0	41.0
47	34.33146173098509	37.0	33.0	40.0	23.0	41.0
48	34.71601683819215	37.0	34.0	40.0	24.0	41.0
49	35.10100299115858	38.0	34.0	40.0	26.0	41.0
50	34.780768382668974	38.0	34.0	40.0	24.0	41.0
51	34.38629050241747	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	12.0
11	12.0
12	17.0
13	26.0
14	34.0
15	71.0
16	303.0
17	925.0
18	2067.0
19	3928.0
20	6210.0
21	7937.0
22	8344.0
23	8171.0
24	8571.0
25	10548.0
26	13050.0
27	15522.0
28	18897.0
29	24296.0
30	31755.0
31	42402.0
32	56660.0
33	72029.0
34	87275.0
35	103085.0
36	108734.0
37	103140.0
38	61793.0
39	6881.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.809824105922644	2.8390467659732974	37.194764164400354	14.156364963703705
2	16.855134988333113	32.83322889401881	34.79486083824697	15.516775279401122
3	12.6629035292183	32.83023898123705	40.16748494765784	14.33937254188681
4	13.01982436754001	2.7929522772546123	67.05078740598904	17.13643594921633
5	36.915954797501925	3.419089845633295	39.68062748302913	19.984327873835646
6	14.703020683967466	27.734555543236006	42.89515746251085	14.667266310285672
7	57.11281563824049	1.1238334668412444	37.41315237716753	4.350198517750738
8	54.303294260987	23.606108890131917	14.710246306523366	7.3803505423577205
9	51.49364830403427	2.8066560441709782	14.17031789001855	31.529377761776207
10	41.269018648335184	20.56711170687892	21.829602378973938	16.334267265811967
11	32.822265880485716	18.735291809258513	30.09260009044486	18.349842219810913
12	21.49087017674122	14.895122580195066	31.948339290319282	31.665667952744432
13	24.723588792311936	18.012231234871354	42.69670200162203	14.56747797119468
14	19.65344419265503	29.86947784910658	27.290678074844994	23.186399883393403
15	11.788976939051873	21.34100079855587	43.827013612823734	23.04300864956852
16	13.839558788537174	23.909958776577522	25.821758841109805	36.428723593775494
17	14.948816430567375	29.123370030360075	35.72248127878569	20.205332260286855
18	14.197102525355081	23.238349617976354	33.075785568438484	29.488762288230085
19	14.194984670468008	27.20260022748253	29.553294572436243	29.049120529613216
20	18.907710113006246	25.46919829226148	41.074798897220504	14.54829269751177
21	19.32380630846681	35.15489616904967	29.234245962683396	16.287051559800126
22	15.092955142587694	20.13195481743468	38.55953476957116	26.215555270406465
23	18.779891341586325	31.52863028358077	30.074162294957386	19.61731607987552
24	19.71735357836499	25.089105629881185	26.659183579399006	28.534357212354816
25	13.981828805068899	39.39994942064211	23.087483602197086	23.530738172091905
26	14.746000680205157	21.99280178497793	33.84269819695802	29.4184993378589
27	21.801696526344248	29.53373555965561	24.96776500282173	23.69680291117841
28	12.419474796903945	29.6262982761907	34.26614459467372	23.688082332231634
29	20.72134137453765	20.665155930180553	27.949330944725233	30.664171750556562
30	15.3236767455796	35.8904147133608	28.59390630859139	20.19200223246821
31	21.58617364665958	26.553166255346028	23.52338796983676	28.337272128157625
32	29.70964209498205	30.752498757317497	20.315086975317023	19.222772172383422
33	18.876191449098602	23.33539720368407	22.027684100765043	35.760727246452284
34	23.232618951811325	22.385975315778392	27.90597720938982	26.47542852302046
35	23.35196630368295	27.66927578083441	22.602619412756216	26.376138502726427
36	16.105788097406375	34.815541068320755	20.986945293316673	28.091725540956197
37	21.04462569406465	29.84692892354419	30.009380851352745	19.099064531038408
38	20.09607586405365	30.219671383669343	20.460596064028984	29.223656688248024
39	26.1214975974805	25.054846212590277	28.87994129804572	19.943714891883506
40	22.69729998417838	20.984827438429598	26.533856401963874	29.784016175428153
41	17.919668518336263	30.513804053574255	28.5164177356643	23.05010969242518
42	25.785506148631054	21.26924289179381	31.27959546480063	21.665655494774505
43	20.69069476852469	28.71437487775617	22.266378804508292	28.32855154921085
44	20.85912652189675	23.021331781900813	30.939866624973995	25.17967507122844
45	18.843925306995523	21.006379726398063	27.065562558318874	33.084132408287545
46	30.51442695207045	26.64099494331001	23.228258662337936	19.6163194422816
47	13.808787602824971	22.211314577444348	41.28234867615382	22.697549143576857
48	20.182783334724476	26.913949064344166	27.46284721919424	25.440420381737116
49	21.833215190251888	18.212306231850295	36.98522111027919	22.969257467618622
50	23.277218484139137	21.734423488754814	28.383615776274794	26.604742250831258
51	19.54481069491802	18.92203677841881	27.246078542517182	34.28707398414599
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	223.5
2	358.0
3	5216.0
4	10074.0
5	6797.0
6	3520.0
7	3511.0
8	3502.0
9	3722.5
10	3943.0
11	4305.5
12	4668.0
13	4667.5
14	4667.0
15	4436.0
16	4205.0
17	4037.0
18	3869.0
19	3629.0
20	3389.0
21	3357.0
22	3325.0
23	3432.0
24	3539.0
25	3791.5
26	4662.0
27	5280.0
28	5816.5
29	6353.0
30	7396.0
31	8439.0
32	9682.5
33	10926.0
34	12668.5
35	14411.0
36	16035.5
37	17660.0
38	18922.5
39	20185.0
40	22192.0
41	24199.0
42	27394.5
43	30590.0
44	36252.5
45	41915.0
46	128907.0
47	215899.0
48	160045.5
49	104192.0
50	93212.0
51	82232.0
52	64680.5
53	47129.0
54	40831.5
55	34534.0
56	30668.5
57	26803.0
58	23647.5
59	20492.0
60	17286.0
61	14080.0
62	11442.0
63	8804.0
64	7422.5
65	6041.0
66	4957.0
67	3873.0
68	3096.5
69	2320.0
70	1783.5
71	1247.0
72	1072.0
73	897.0
74	684.5
75	348.5
76	225.0
77	203.5
78	182.0
79	142.5
80	103.0
81	58.5
82	14.0
83	10.5
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	802699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.280373771871055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.32959064743449	25.73557680372404
2	9.252833762347278	6.158755321198107
3	3.3346960078469308	3.3293978867013636
4	1.8035055626167953	2.4008535689414217
5	1.2947144428821777	2.1544290293479333
6	0.9583092684370699	1.9135734385580403
7	0.7954429595976947	1.8530847306730227
8	0.726249745470486	1.9335890384787198
9	0.588108361047078	1.7615219466608323
>10	3.77751174911641	19.69469900467222
>50	0.0824347980103707	1.9014586056970688
>100	0.041787224639269614	2.8247532914067217
>500	0.006078141765711944	1.3276074973397478
>1k	0.004938490184640955	3.4547378794691888
>5k	0.0018994193017849827	4.5999115496818765
>10k+	0.0018994193017849827	18.956050407449744
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGC	49182	6.127078768006438	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCG	41535	5.174417807920529	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTC	31420	3.9142941501110626	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAGCTGAT	15976	1.990285275053289	No Hit
GCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	11824	1.4730303638100957	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTA	8522	1.061668196920639	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCT	7967	0.992526463842611	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTC	6990	0.8708120976854339	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6783	0.8450240999428179	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	6122	0.7626769187453828	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAAGCTGATCGT	3328	0.41460123906968865	No Hit
CTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCT	3159	0.39354726989818095	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	3097	0.38582332854532025	No Hit
CCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2772	0.34533492629242096	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATG	2734	0.3406008977213127	No Hit
TCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGC	2031	0.2530213691558106	No Hit
CGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTG	2013	0.2507789345694962	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTAT	1931	0.24056339923184156	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAAGCTGA	1798	0.22399429923296277	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGCTGATCGTATGCCGTCTTC	1195	0.14887274059142966	No Hit
TGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTTCTGCTT	1159	0.14438787141880083	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCAAGCTGATCG	1066	0.13280195938950964	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCC	1043	0.12993662630699676	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCTT	870	0.1083843383385304	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCAAGCTGATCGTATGCCGTCT	817	0.10178161427882682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4418841932031808	0.0
2	0.0	0.0	0.0	2.7032548938020353	0.0
3	0.0	0.0	0.0	3.367140111050344	0.0
4	0.0	0.0	0.0	5.206559370324368	0.0
5	0.0	0.0	0.0	10.654055878978298	0.0
6	0.0	0.0	0.0	11.799067894690289	0.0
7	0.0	0.0	0.0	13.356189555487175	0.0
8	0.0	0.0	0.0	15.170568295214022	0.0
9	0.0	0.0	0.0	15.684459554577744	0.0
10	0.0	0.0	0.0	21.99317552407565	0.0
11	0.0	0.0	0.0	23.546559793895344	0.0
12	0.0	0.0	0.0	31.418003510655925	0.0
13	0.0	0.0	0.0	32.243717757216594	0.0
14	0.0	0.0	0.0	32.6508442143319	0.0
15	0.0	0.0	0.0	34.055231163860924	0.0
16	0.0	0.0	0.0	34.89464917733795	0.0
17	0.0	0.0	0.0	35.693578788562085	0.0
18	0.0	0.0	0.0	36.3405211667138	0.0
19	0.0	0.0	0.0	39.1227595898338	0.0
20	0.0	0.0	0.0	39.87895836421872	0.0
21	0.0	0.0	0.0	40.54446311755714	0.0
22	0.0	0.0	0.0	41.36108304607331	0.0
23	0.0	0.0	0.0	42.09572953248976	0.0
24	1.245796992396901E-4	0.0	0.0	42.681752437713264	0.0
25	1.245796992396901E-4	0.0	0.0	43.17097691662753	0.0
26	1.245796992396901E-4	0.0	0.0	43.68412069779581	0.0
27	1.245796992396901E-4	0.0	0.0	44.20486384061772	0.0
28	1.245796992396901E-4	0.0	0.0	44.64201400524979	0.0
29	1.245796992396901E-4	0.0	0.0	45.12563239769827	0.0
30	1.245796992396901E-4	0.0	0.0	45.66219716232361	0.0
31	1.245796992396901E-4	0.0	0.0	46.137094975825306	0.0
32	1.245796992396901E-4	0.0	0.0	46.55505986677447	0.0
33	1.245796992396901E-4	0.0	0.0	46.986479365241514	0.0
34	1.245796992396901E-4	0.0	0.0	47.429235616339376	0.0
35	1.245796992396901E-4	0.0	0.0	47.8790929102939	0.0
36	1.245796992396901E-4	0.0	0.0	48.31562017642977	0.0
37	1.245796992396901E-4	0.0	0.0	48.756133992941315	0.0
38	2.491593984793802E-4	0.0	0.0	49.199388562836134	0.0
39	2.491593984793802E-4	0.0	0.0	49.82216247933534	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAG	20	7.032012E-4	45.000004	26
TCTACGG	20	7.032012E-4	45.000004	2
ACGGACG	40	6.8102963E-9	45.000004	43
CGGTGTA	20	7.032012E-4	45.000004	19
AGACACG	20	7.032012E-4	45.000004	24
GGACGCA	20	7.032012E-4	45.000004	8
CTCGAAT	20	7.032012E-4	45.000004	43
ACCAACG	20	7.032012E-4	45.000004	36
CCAACGC	20	7.032012E-4	45.000004	37
CCCTACG	25	3.889751E-5	45.0	31
GCGCGAC	45	3.8380676E-10	45.0	9
TCGGGTC	70	0.0	45.0	5
TATGCGG	35	1.2113196E-7	45.0	2
CGGTGAT	25	3.889751E-5	45.0	31
TCGTAAC	25	3.889751E-5	45.0	34
CGAGCGT	25	3.889751E-5	45.0	28
CGATCGA	140	0.0	45.0	41
TACCGCG	35	1.2113196E-7	45.0	29
CGGGTCC	60	0.0	44.999996	6
CGAATAC	60	0.0	44.999996	45
>>END_MODULE