SRR2933816.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933816.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421609
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	30494	7.232767801446364	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCG	24581	5.830283509128126	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTC	19610	4.651228982303509	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGATACGT	9431	2.23690670739951	No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	8953	2.1235315185396897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTA	5960	1.4136320619341618	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCT	5813	1.3787656335609533	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTC	4594	1.0896351833096543	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT	4194	0.9947605482805159	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	2494	0.5915433494066777	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	2380	0.5645040784233734	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGATACGTCGT	2040	0.48386063864860573	No Hit
TCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	1939	0.4599047933037482	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATG	1863	0.441878612648212	No Hit
TGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCTT	1445	0.34273461904276237	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTAT	1331	0.3156953480594579	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.28391234532469656	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT	1182	0.28035454651110386	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGATACG	1071	0.254026835290518	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	702	0.16650498447613785	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTGATACGTCG	562	0.13329886221593942	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	551	0.1306898097526381	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	448	0.10625959123263498	No Hit
TCCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	442	0.1048364717071979	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATGGG	65	0.0	45.000004	3
GCAAGGC	20	7.0281996E-4	45.0	15
GTCGAAT	25	3.8865895E-5	45.0	43
CCGATCA	20	7.0281996E-4	45.0	40
GCGCGTT	20	7.0281996E-4	45.0	32
TTAGCGG	35	1.2094824E-7	45.0	2
GCAACTA	20	7.0281996E-4	45.0	9
TTTGTCG	40	6.7975634E-9	45.0	1
GTTTACG	35	1.2094824E-7	45.0	1
TAGGTAG	20	7.0281996E-4	45.0	29
TTGTCTA	20	7.0281996E-4	45.0	43
GGGTACG	20	7.0281996E-4	45.0	7
TTCGTTG	45	3.8380676E-10	45.0	1
GTGATCC	25	3.8865895E-5	45.0	14
ATAGATC	20	7.0281996E-4	45.0	32
ATTCGGC	35	1.2094824E-7	45.0	28
CTCTTCG	35	1.2094824E-7	45.0	1
CGATTGA	25	3.8865895E-5	45.0	20
GCGTTAG	25	3.8865895E-5	45.0	43
TCGGCGA	35	1.2094824E-7	45.0	30

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content