##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933816.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421609
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.54935259920922	28.0	16.0	31.0	16.0	33.0
2	25.361372741094236	28.0	16.0	31.0	16.0	34.0
3	26.427633186198587	30.0	16.0	33.0	16.0	34.0
4	30.9083463588301	33.0	28.0	35.0	19.0	37.0
5	33.161758880858805	35.0	32.0	35.0	28.0	37.0
6	31.268480985937206	35.0	28.0	36.0	17.0	37.0
7	33.017981115203895	35.0	32.0	35.0	28.0	37.0
8	31.791681392000644	35.0	30.0	36.0	17.0	37.0
9	34.053931486282316	35.0	32.0	38.0	28.0	39.0
10	33.368720781577245	35.0	32.0	37.0	27.0	39.0
11	34.00505444618118	35.0	32.0	38.0	27.0	39.0
12	34.23498312417429	35.0	32.0	39.0	27.0	39.0
13	34.37460063708318	35.0	33.0	37.0	27.0	39.0
14	34.34535553083544	36.0	32.0	38.0	27.0	40.0
15	34.92841471600464	37.0	33.0	39.0	27.0	40.0
16	34.54114119954745	36.0	32.0	39.0	27.0	40.0
17	33.976774689344865	36.0	32.0	38.0	27.0	40.0
18	34.232862676081396	36.0	32.0	38.0	27.0	40.0
19	34.06092374688396	36.0	32.0	39.0	27.0	40.0
20	33.82168075159686	35.0	31.0	39.0	25.0	40.0
21	33.7926040478263	36.0	32.0	39.0	25.0	40.0
22	35.09764971810374	37.0	33.0	39.0	27.0	40.0
23	35.342739362774516	37.0	33.0	39.0	29.0	40.0
24	35.659722634004495	37.0	34.0	39.0	30.0	40.0
25	33.7774573123439	36.0	32.0	39.0	24.0	40.0
26	34.725487359140814	37.0	33.0	39.0	27.0	40.0
27	34.86810765424837	37.0	33.0	39.0	27.0	40.0
28	34.938922081834114	37.0	33.0	39.0	27.0	40.0
29	35.42676508328807	37.0	34.0	40.0	27.0	40.0
30	33.78765396374366	36.0	32.0	39.0	24.0	40.0
31	34.58910744315231	37.0	33.0	39.0	25.0	40.0
32	34.79557836763447	37.0	33.0	39.0	27.0	40.0
33	35.121505945081815	37.0	34.0	39.0	27.0	40.0
34	35.2941920120301	38.0	34.0	40.0	27.0	40.0
35	35.52994599261401	38.0	34.0	40.0	29.0	41.0
36	35.49388888757119	38.0	34.0	40.0	29.0	41.0
37	35.36660270535022	38.0	34.0	40.0	28.0	41.0
38	35.24937560630821	37.0	34.0	40.0	29.0	41.0
39	35.266673624139905	37.0	34.0	40.0	29.0	40.0
40	34.89851971850696	37.0	33.0	40.0	27.0	40.0
41	34.21901097936714	37.0	33.0	39.0	25.0	40.0
42	35.22492641286121	37.0	34.0	40.0	29.0	40.0
43	35.3017464048443	38.0	34.0	40.0	29.0	40.0
44	35.93988505937966	38.0	35.0	40.0	30.0	41.0
45	36.24062342122678	38.0	35.0	40.0	31.0	41.0
46	35.96884554172231	38.0	35.0	40.0	30.0	41.0
47	35.23168385874116	38.0	34.0	40.0	26.0	41.0
48	35.65237933725324	38.0	35.0	40.0	29.0	41.0
49	35.9595525712212	38.0	35.0	40.0	30.0	41.0
50	35.58917385539682	38.0	35.0	40.0	28.0	41.0
51	35.25415965977956	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	8.0
12	10.0
13	7.0
14	17.0
15	34.0
16	108.0
17	293.0
18	659.0
19	1094.0
20	1682.0
21	2109.0
22	2525.0
23	2726.0
24	3070.0
25	3829.0
26	5206.0
27	7043.0
28	9430.0
29	12489.0
30	17175.0
31	23437.0
32	31604.0
33	41133.0
34	50028.0
35	58390.0
36	61150.0
37	53909.0
38	29469.0
39	2967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.32603431141176	2.977166047214362	34.85148561819126	12.845314023182617
2	14.63227777395644	35.388476052456184	33.387807186279225	16.591438987308145
3	13.643446890365244	35.68851708573584	35.85502207021197	14.813013953686946
4	14.883221183608509	3.0862718774978712	62.96592340296341	19.06458353593021
5	38.19700243590625	3.768894876532522	36.218629108961146	21.815473578600077
6	15.822005697221833	29.562936275079515	38.47593386289192	16.139124164806727
7	55.13829163988435	1.7418982991349803	37.89672421603903	5.22308584494164
8	49.96975871008446	25.170477859817986	14.587923882080316	10.271839548017239
9	46.890602430213775	3.9906643359131326	14.431380734282238	34.68735249959085
10	41.16444383303013	18.883135796436985	22.118835224105748	17.83358514642714
11	34.24974324551896	19.114867092495654	27.405012701341764	19.230376960643632
12	23.99687862450754	14.667618575504793	29.943857934721507	31.391644865266162
13	23.52345419571214	19.84136961023128	41.68032466100107	14.954851533055507
14	19.62861324117844	30.092099551954536	23.893939645500925	26.385347561366103
15	11.471292121373121	22.199478663880516	44.90606225199178	21.42316696275459
16	12.87140454781563	23.64750278101274	24.528176580670717	38.95291609050091
17	13.871620387610323	28.191286239145747	35.78220578782711	22.15488758541682
18	13.40293969056638	23.248080567540068	32.6394835024869	30.709496239406654
19	14.19419414670939	27.932515672103776	28.26528845446848	29.608001726718356
20	17.950755320688124	24.709624320163943	43.08328332649445	14.256337032653477
21	20.444772289016598	35.63941946210826	26.285966381173075	17.629841867702066
22	14.817520498850831	20.994570799010457	34.97553420349186	29.21237449864685
23	17.625572509125753	33.31143310508077	28.32245042207353	20.74054396371994
24	22.15725945129255	21.921021610069992	24.278893477131653	31.6428254615058
25	13.36712451584288	39.11728639568889	20.76900635422868	26.746582734239542
26	15.286438382482348	20.68883728762906	31.234627344292935	32.79009698559566
27	21.018052271180167	28.150727332670794	24.984760761748444	25.846459634400592
28	14.814437073212384	25.839344036773408	34.697551522856486	24.648667367157724
29	20.33946144413426	20.50051113709622	26.53809572376301	32.62193169500651
30	16.936071099051492	28.66328754841571	36.71648375627655	17.68415759625625
31	27.63484650469985	23.97932681702715	19.66134499026349	28.724481688009508
32	22.96416822221537	35.08985813870197	21.155857678559993	20.790115960522666
33	20.08875522106976	20.236996838302787	26.561577195932724	33.112670744694725
34	24.868064960662604	26.767455153945956	21.2623544563802	27.10212542901124
35	17.224727176127644	20.06788280136335	28.87035143936681	33.837038583142196
36	28.035929024285533	20.82640550842131	22.015421871923987	29.12224359536917
37	15.89411041984398	26.84714984737043	38.40216883415676	18.856570898628824
38	28.01221036552825	21.243616715961945	25.74589252126971	24.998280397240098
39	21.694271232350353	36.035521063354906	24.410532033234585	17.859675671060153
40	16.31677691889879	19.670358080591257	35.800706341657786	28.21215865885216
41	24.480502076568573	31.106546587003596	21.15063957363339	23.26231176279444
42	19.140720430541094	18.097336631808144	31.206402140371765	31.555540797278997
43	30.7151887175084	17.46262532346321	22.526796154731045	29.295389804297344
44	21.14803052117009	18.58285757656976	33.98883799918882	26.280273903071325
45	20.33898707095911	15.554222039852089	27.468578706811286	36.63821218237751
46	30.791799985294432	26.09052463301305	23.735736191589837	19.381939190102678
47	13.444210156804054	17.957633731727736	47.7743596555102	20.823796455958007
48	21.517567224608584	25.75277093230932	25.08793692734263	27.641724915739463
49	18.225891762272628	15.106888135689703	40.30725150554186	26.359968596495804
50	27.331010486019036	15.098823791712226	29.67014461266244	27.900021109606293
51	19.568130661347364	15.67400126657638	27.071765545801917	37.68610252627435
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	184.5
2	321.0
3	1204.0
4	2087.0
5	1496.0
6	905.0
7	934.5
8	964.0
9	1156.5
10	1349.0
11	1340.0
12	1331.0
13	1272.5
14	1214.0
15	1150.5
16	1087.0
17	1122.0
18	1157.0
19	1092.5
20	1028.0
21	1135.0
22	1242.0
23	1498.5
24	1755.0
25	1691.0
26	2103.5
27	2580.0
28	2829.5
29	3079.0
30	3463.0
31	3847.0
32	4605.0
33	5363.0
34	5736.5
35	6110.0
36	6592.0
37	7074.0
38	7981.5
39	8889.0
40	10168.5
41	11448.0
42	12943.5
43	14439.0
44	17137.0
45	19835.0
46	74601.5
47	129368.0
48	93281.0
49	57194.0
50	51811.5
51	46429.0
52	34759.5
53	23090.0
54	19231.5
55	15373.0
56	13691.0
57	12009.0
58	11499.5
59	10990.0
60	9964.5
61	8939.0
62	8058.5
63	7178.0
64	5882.5
65	4587.0
66	3658.0
67	2729.0
68	2230.5
69	1732.0
70	1484.5
71	1237.0
72	1009.5
73	782.0
74	670.0
75	421.0
76	284.0
77	243.5
78	203.0
79	144.5
80	86.0
81	58.0
82	30.0
83	30.5
84	31.0
85	16.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	421609.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.74900116871387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.9599539797259	17.56187983976119
2	11.972851326409993	5.926322229403167
3	4.4065150949718	3.271705417062371
4	2.3385710887740188	2.315091944367546
5	1.3614089253555604	1.6846755542361127
6	0.9526891968569281	1.4146863627859918
7	0.7693133919132309	1.3327816624897448
8	0.6447465962625917	1.2765467411541738
9	0.5969134973237327	1.3295711558597532
>10	5.755879885454604	24.659830962423605
>50	0.13353322426248837	2.2610846305321113
>100	0.08570042751174627	4.3154950769034555
>500	0.002989549796921381	0.4476296470785102
>1k	0.011958199187685524	6.345735988611607
>5k	0.003986066395895175	7.437557722835611
>10k+	0.002989549796921381	18.419405064495063
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	30494	7.232767801446364	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCG	24581	5.830283509128126	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTC	19610	4.651228982303509	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGATACGT	9431	2.23690670739951	No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	8953	2.1235315185396897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTA	5960	1.4136320619341618	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCT	5813	1.3787656335609533	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTC	4594	1.0896351833096543	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT	4194	0.9947605482805159	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	2494	0.5915433494066777	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	2380	0.5645040784233734	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGATACGTCGT	2040	0.48386063864860573	No Hit
TCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	1939	0.4599047933037482	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATG	1863	0.441878612648212	No Hit
TGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCTT	1445	0.34273461904276237	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTAT	1331	0.3156953480594579	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1197	0.28391234532469656	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT	1182	0.28035454651110386	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGATACG	1071	0.254026835290518	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	702	0.16650498447613785	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTGATACGTCG	562	0.13329886221593942	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	551	0.1306898097526381	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	448	0.10625959123263498	No Hit
TCCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	442	0.1048364717071979	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7023094858031969	0.0
2	0.0	0.0	0.0	4.078897746490232	0.0
3	0.0	0.0	0.0	4.894582421153249	0.0
4	0.0	0.0	0.0	7.462838791392024	0.0
5	0.0	0.0	0.0	14.043817850188207	0.0
6	0.0	0.0	0.0	15.096214739248925	0.0
7	0.0	0.0	0.0	16.881280997322165	0.0
8	0.0	0.0	0.0	19.115815838845943	0.0
9	0.0	0.0	0.0	19.56860503452251	0.0
10	0.0	0.0	0.0	26.756781757505177	0.0
11	0.0	0.0	0.0	28.490852899250253	0.0
12	0.0	0.0	0.0	37.806830499348926	0.0
13	0.0	0.0	0.0	38.75842308869118	0.0
14	0.0	0.0	0.0	39.25343149695571	0.0
15	0.0	0.0	0.0	41.12246180702974	0.0
16	0.0	0.0	0.0	42.163236553299384	0.0
17	0.0	0.0	0.0	43.03679475533018	0.0
18	0.0	0.0	0.0	43.737681121607935	0.0
19	0.0	0.0	0.0	46.89249992291436	0.0
20	0.0	0.0	0.0	47.720043926956016	0.0
21	0.0	0.0	0.0	48.4294690103864	0.0
22	0.0	0.0	0.0	49.27907136707233	0.0
23	0.0	0.0	0.0	49.95932250023126	0.0
24	0.0	0.0	0.0	50.52691000429308	0.0
25	0.0	0.0	0.0	51.011719389291976	0.0
26	0.0	0.0	0.0	51.480874459511064	0.0
27	0.0	0.0	0.0	51.96022855299579	0.0
28	0.0	0.0	0.0	52.38858753015234	0.0
29	0.0	0.0	0.0	52.83900485995318	0.0
30	0.0	0.0	0.0	53.309820236285276	0.0
31	0.0	0.0	0.0	53.79889897986049	0.0
32	0.0	0.0	0.0	54.232950435118795	0.0
33	0.0	0.0	0.0	54.67696372705516	0.0
34	0.0	0.0	0.0	55.10389958468628	0.0
35	0.0	0.0	0.0	55.50948864943585	0.0
36	0.0	0.0	0.0	55.93428982777882	0.0
37	0.0	0.0	0.0	56.35363571460761	0.0
38	0.0	0.0	0.0	56.77535346731213	0.0
39	0.0	0.0	0.0	57.21604614702248	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCATGGG	65	0.0	45.000004	3
GCAAGGC	20	7.0281996E-4	45.0	15
GTCGAAT	25	3.8865895E-5	45.0	43
CCGATCA	20	7.0281996E-4	45.0	40
GCGCGTT	20	7.0281996E-4	45.0	32
TTAGCGG	35	1.2094824E-7	45.0	2
GCAACTA	20	7.0281996E-4	45.0	9
TTTGTCG	40	6.7975634E-9	45.0	1
GTTTACG	35	1.2094824E-7	45.0	1
TAGGTAG	20	7.0281996E-4	45.0	29
TTGTCTA	20	7.0281996E-4	45.0	43
GGGTACG	20	7.0281996E-4	45.0	7
TTCGTTG	45	3.8380676E-10	45.0	1
GTGATCC	25	3.8865895E-5	45.0	14
ATAGATC	20	7.0281996E-4	45.0	32
ATTCGGC	35	1.2094824E-7	45.0	28
CTCTTCG	35	1.2094824E-7	45.0	1
CGATTGA	25	3.8865895E-5	45.0	20
GCGTTAG	25	3.8865895E-5	45.0	43
TCGGCGA	35	1.2094824E-7	45.0	30
>>END_MODULE