FastQCFastQC Report
Sat 14 Jan 2017
SRR2933815.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933815.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397840
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC319618.033631610697768No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG271396.8215865674643075No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC212395.338578322943897No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAATCGGT103642.6050673637643276No Hit
GCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC95732.4062437160667605No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTA62341.5669615926000402No Hit
GAACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCT55971.4068469736577518No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTC51601.2970038206314096No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT49621.2472350693746228No Hit
CTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCT31120.7822240096521214Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC29220.7344661170319727No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGT22480.5650512768952343No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATG20080.504725517796099No Hit
TCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGC18920.47556806756485015No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTAT14830.37276291976674036No Hit
TGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTGCTT14140.355419264025739Illumina Single End Adapter 1 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13850.3481299014679268No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGAATCGG12520.31469937663382264No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTT11610.29182585964206714No Hit
CGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG8690.21842951940478583No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAGAATCGGTCG6190.1555901870098532No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC5390.13548160064347478No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGC5050.12693545143776394No Hit
TCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTCTG4770.11989744620953147No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCC4760.11964608887995173No Hit
GAATGACTCTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCT4740.11914337422079227No Hit
GAACTGTCTCTTATACACATCTGACGCAGCATCGGTCGTATGCCGTCTTCT4710.1183893022320531No Hit
GTTCCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCGTCTTC4670.11738387291373416No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAGAATCGGTCGTATGCCG4070.10230243313895032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCG207.0277194E-445.01
ATGGGCG207.0277194E-445.05
CGGGTCA207.0277194E-445.06
CGGGTAA453.8380676E-1045.06
CCGGGTA253.8861916E-545.05
CCGGGAT700.045.05
CGAACCC253.8861916E-545.034
ACACGAT253.8861916E-545.035
CTTCCGT207.0277194E-445.012
CGCGGGC253.8861916E-545.04
CGACGGA207.0277194E-445.041
TTAGCGG253.8861916E-545.02
CGTGAGT253.8861916E-545.023
TATGGGA253.8861916E-545.04
CGTTGAC207.0277194E-445.011
GATCCGT253.8861916E-545.05
CCTACCC207.0277194E-445.036
TCCTCGG351.209246E-745.02
CACGATC253.8861916E-545.036
AGGAACG453.8380676E-1045.043
Produced by FastQC (version 0.11.5)