SRR2933814.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933814.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257569
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	21385	8.302629586635039	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	17440	6.771001168618894	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	14542	5.645865768007797	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGGAAT	6894	2.676564338099694	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	6617	2.5690203401806895	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTA	4512	1.7517636050922276	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	4492	1.7439986954951876	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTC	3461	1.3437176057677749	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCT	3297	1.2800453470720468	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	2247	0.8723875932274459	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	2094	0.8129860348100898	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGT	1557	0.6044982121295653	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1403	0.5447084082323571	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATG	1213	0.470941767060477	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTAT	992	0.3851395160131848	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	905	0.35136215926606074	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAGGAA	823	0.3195260299181967	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	681	0.26439517177921257	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.25080657998439254	No Hit
TGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCTT	616	0.2391592155888325	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	584	0.2267353602335685	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGGTAGGAATCG	440	0.17082801113488036	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	418	0.16228661057813634	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	359	0.1393801272668683	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	338	0.13122697218997628	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	308	0.11957960779441625	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	296	0.11492066203619225	No Hit
GCACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	291	0.11297943463693225	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	288	0.11181469819737623	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	271	0.10521452503989223	No Hit
GACTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	264	0.10249680668092821	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	262	0.10172031572122421	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCC	20	7.023083E-4	45.000004	35
ACTGCGA	20	7.023083E-4	45.000004	30
GATGTGA	20	7.023083E-4	45.000004	10
CAAAATA	20	7.023083E-4	45.000004	37
CGCGGGT	20	7.023083E-4	45.000004	4
GGGTAAT	20	7.023083E-4	45.000004	7
TCCCTTG	20	7.023083E-4	45.000004	1
GAATCTA	20	7.023083E-4	45.000004	9
ATGCTCC	40	6.7793735E-9	45.000004	3
GGTTTCG	20	7.023083E-4	45.000004	1
CGATTTC	20	7.023083E-4	45.000004	10
CGCGATA	20	7.023083E-4	45.000004	43
TACGGGC	20	7.023083E-4	45.000004	29
AGGCGAA	20	7.023083E-4	45.000004	31
TCGGCGA	20	7.023083E-4	45.000004	30
GGGACGG	20	7.023083E-4	45.000004	7
TCTCGTG	20	7.023083E-4	45.000004	1
TAGGGTG	40	6.7793735E-9	45.000004	5
GCGATGT	20	7.023083E-4	45.000004	9
CGTACCT	20	7.023083E-4	45.000004	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content