##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933814.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	257569
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.74058601772729	25.0	16.0	31.0	16.0	33.0
2	24.363184234127555	27.0	16.0	31.0	16.0	34.0
3	25.613866575558394	30.0	16.0	33.0	16.0	34.0
4	30.318617535495342	32.0	28.0	35.0	19.0	37.0
5	32.90920102962701	35.0	32.0	35.0	28.0	37.0
6	30.758503546622457	35.0	28.0	35.0	17.0	37.0
7	32.938478621262654	33.0	32.0	35.0	28.0	37.0
8	31.041010370036766	35.0	28.0	35.0	17.0	37.0
9	33.95710275693115	35.0	32.0	37.0	28.0	39.0
10	33.358560230462516	34.0	32.0	37.0	27.0	39.0
11	34.08009892494827	35.0	32.0	37.0	27.0	39.0
12	34.3043029246532	35.0	33.0	39.0	27.0	39.0
13	34.44120992821341	35.0	33.0	37.0	27.0	39.0
14	34.2051411466442	36.0	32.0	38.0	25.0	40.0
15	34.857742973727426	36.0	32.0	39.0	27.0	40.0
16	34.55835911930395	36.0	32.0	38.0	27.0	40.0
17	33.89412545764436	36.0	32.0	38.0	27.0	40.0
18	34.24918371387861	36.0	32.0	38.0	27.0	40.0
19	33.92264985304909	36.0	32.0	39.0	27.0	40.0
20	33.36426743901634	35.0	31.0	38.0	25.0	40.0
21	33.5876056513012	36.0	32.0	38.0	25.0	40.0
22	34.94895736676386	37.0	33.0	39.0	27.0	40.0
23	35.088679150053	37.0	33.0	39.0	27.0	40.0
24	35.48367621879962	37.0	34.0	39.0	29.0	40.0
25	33.635538438243735	36.0	32.0	39.0	24.0	40.0
26	34.657101592194714	37.0	33.0	39.0	27.0	40.0
27	34.31052261724043	36.0	33.0	39.0	27.0	40.0
28	34.467664975210525	37.0	33.0	39.0	25.0	40.0
29	34.948072167069796	37.0	33.0	39.0	27.0	40.0
30	33.31170676595398	36.0	31.0	39.0	23.0	40.0
31	34.08516552846034	36.0	32.0	39.0	25.0	40.0
32	34.01996358257399	36.0	32.0	39.0	25.0	40.0
33	34.5582193509312	37.0	33.0	39.0	25.0	40.0
34	34.58119960088365	37.0	33.0	39.0	25.0	40.0
35	34.828570208371346	37.0	33.0	40.0	25.0	40.0
36	34.477184754376495	37.0	33.0	40.0	25.0	40.0
37	34.66854318648595	37.0	33.0	40.0	25.0	40.0
38	33.95674945354449	37.0	32.0	39.0	23.0	40.0
39	34.21314676843875	37.0	33.0	39.0	24.0	40.0
40	34.00035330338667	37.0	32.0	39.0	24.0	40.0
41	33.43713335067496	36.0	31.0	39.0	23.0	40.0
42	33.95369784407285	37.0	33.0	39.0	23.0	40.0
43	34.365420528091505	37.0	33.0	39.0	25.0	40.0
44	35.00127732762871	37.0	34.0	40.0	27.0	40.0
45	34.91722218124075	37.0	34.0	40.0	26.0	40.0
46	34.863050289437005	37.0	34.0	40.0	26.0	40.0
47	33.76463782520412	37.0	32.0	40.0	22.0	41.0
48	34.375188784364575	37.0	33.0	40.0	26.0	40.0
49	34.61788491627486	37.0	34.0	40.0	26.0	41.0
50	34.39121555777287	37.0	33.0	40.0	26.0	41.0
51	33.93645974476742	36.0	32.0	40.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	4.0
11	11.0
12	1.0
13	5.0
14	14.0
15	35.0
16	98.0
17	355.0
18	754.0
19	1334.0
20	1768.0
21	2005.0
22	2098.0
23	2132.0
24	2369.0
25	2928.0
26	4021.0
27	5226.0
28	6839.0
29	9069.0
30	12092.0
31	16184.0
32	21403.0
33	26298.0
34	30371.0
35	33991.0
36	33208.0
37	27501.0
38	14135.0
39	1319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	55.143281994339375	3.0818926190651825	29.714367800472886	12.060457586122554
2	12.981764109811353	42.49307952432164	28.9902899805489	15.534866385318107
3	11.84459309932484	42.23023733446183	32.386661438294205	13.53850812791912
4	13.513660417208593	2.3748976002546893	67.41882757629995	16.692614406236775
5	42.72214435743432	3.03685614340235	33.236530793690235	21.004468705473098
6	13.920153434613638	34.72156975412414	35.97676739048566	15.381509420776569
7	48.49729587023283	1.1096055814170183	44.671524911771215	5.7215736365789365
8	42.14831753821306	29.808323206597066	16.023667444451778	12.019691810738093
9	39.77147871055911	3.1634241698341024	16.215072466018814	40.850024653587965
10	42.82852361891376	15.553502168351004	23.15690164577259	18.46107256696264
11	36.426743901634126	17.493953076651305	27.174465871281093	18.904837150433476
12	21.704475305646255	13.623145642526858	30.888422131545333	33.783956920281554
13	23.104100260512716	20.971079594205825	42.99158672045161	12.93323342482985
14	18.361681724120526	33.801816212354744	22.173864090787323	25.6626379727374
15	8.448221641579538	21.830266841118302	46.69583684371955	23.025674673582614
16	9.320609234806984	24.04404256723441	23.81497773412173	42.82037046383687
17	11.400828515854004	30.018364011197	35.72207835570274	22.858729117246252
18	10.90736851096211	23.07498184952382	32.88827459826299	33.12937504125108
19	11.359674494989692	27.32859932678234	28.07519538453774	33.236530793690235
20	17.069600767173068	23.936110323835553	44.61406458075312	14.380224328238258
21	19.114877955033407	38.074069472646165	25.72436900403387	17.086683568286556
22	12.053469167485217	20.666695138001856	36.519922816798605	30.759912877714324
23	17.1534617908211	34.8310549794424	27.20863147350807	20.80685175622843
24	21.844631923872825	22.907259802227752	22.58462780847074	32.66348046542868
25	12.24021524329403	42.0097139019059	19.49497027980852	26.25510057499156
26	18.848929801334787	19.616491115002194	31.699078693476313	29.83550039018671
27	26.73303076068937	28.382297559100667	20.181388288186856	24.70328339202311
28	13.995861303184778	26.561814504074636	37.861310949687265	21.581013243053317
29	31.125640119734904	18.947155907737347	23.983476272377498	25.943727700150248
30	26.947730511047524	30.704005528615635	27.583676607045106	14.76458735329174
31	28.678917105707598	21.49016380076795	22.764385465642217	27.06653362788224
32	30.439610356836422	41.00260512716981	14.895037834522013	13.66274668147176
33	29.083080650233526	21.359325074057825	16.973704133649626	32.58389014205902
34	39.482624073549225	24.137997973358598	19.76130667898699	16.61807127410519
35	27.465261735690245	29.39523001603454	17.661286878467518	25.478221369807702
36	30.91288159677601	27.373247556965318	24.765402668799428	16.948468177459244
37	26.7489488253633	27.28084513276054	30.70788798341415	15.262318058462004
38	27.000143650827546	24.62369306865345	25.56790607565351	22.80825720486549
39	31.164076422240257	33.00474824221859	21.960329076868724	13.870846258672435
40	30.780878133626327	25.876561232135852	23.466333293214632	19.87622734102318
41	28.705317798337532	31.263855510562216	18.770115968924834	21.260710722175418
42	19.486428879251775	28.599326782337936	31.126804856174463	20.78743948223583
43	29.002713835904164	27.91912070163723	18.47466115875746	24.603504303701143
44	28.08956046729226	16.669707922925507	30.33128986795771	24.90944174182452
45	19.95931187371151	15.748013153756856	29.185189211434604	35.107485761097024
46	33.36581653848095	28.889346155787383	22.30353808105789	15.441299224673777
47	16.917796784550937	18.23627843412833	45.995053752586685	18.850871028734048
48	25.81405370987968	23.583971673609792	23.13748937177999	27.46448524473054
49	19.888651196378447	17.120849170513534	41.49567688658185	21.494822746526175
50	26.54977889419922	18.407882936222915	28.42578105284409	26.61655711673377
51	21.603143235404882	15.33297873579507	25.95343383714655	37.1104441916535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	94.5
2	143.0
3	623.0
4	1103.0
5	787.0
6	471.0
7	583.0
8	695.0
9	869.0
10	1043.0
11	1077.0
12	1111.0
13	1082.0
14	1053.0
15	914.5
16	776.0
17	767.5
18	759.0
19	715.5
20	672.0
21	719.0
22	766.0
23	869.0
24	972.0
25	1031.5
26	1240.5
27	1390.0
28	1663.0
29	1936.0
30	1855.5
31	1775.0
32	1939.5
33	2104.0
34	2404.5
35	2705.0
36	3047.0
37	3389.0
38	3528.5
39	3668.0
40	4445.5
41	5223.0
42	6330.5
43	7438.0
44	9150.5
45	10863.0
46	49910.0
47	88957.0
48	63267.0
49	37577.0
50	33863.0
51	30149.0
52	21808.0
53	13467.0
54	11198.5
55	8930.0
56	8198.5
57	7467.0
58	6601.5
59	5736.0
60	5160.5
61	4585.0
62	3997.0
63	3409.0
64	2793.0
65	2177.0
66	1793.5
67	1410.0
68	1186.0
69	962.0
70	777.5
71	593.0
72	504.5
73	416.0
74	321.5
75	211.0
76	195.0
77	130.5
78	66.0
79	52.5
80	39.0
81	20.0
82	1.0
83	7.0
84	13.0
85	7.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	257569.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.91150332532253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.24662870842074	19.756647733228768
2	9.955049445609829	5.159005936273387
3	3.491159724303267	2.7138359041654856
4	1.7845370092897814	1.8496014660149318
5	1.24213365298172	1.6092775139865436
6	0.7836379982019779	1.2183143157755787
7	0.6577764459094996	1.1930783595851984
8	0.5483967635600839	1.1367827650066584
9	0.5124363200479473	1.1950195869844586
>10	4.547497752472281	20.52576202881558
>50	0.11687144141444411	2.0771133172082044
>100	0.08240934971531315	4.1383085697424775
>500	0.010488462691039856	2.0371240327834483
>1k	0.013485166317051244	9.425047268887173
>5k	0.0029967036260113876	5.245584678280383
>10k+	0.004495055439017081	20.719496523261725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	21385	8.302629586635039	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	17440	6.771001168618894	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	14542	5.645865768007797	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGGAAT	6894	2.676564338099694	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	6617	2.5690203401806895	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTA	4512	1.7517636050922276	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	4492	1.7439986954951876	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTC	3461	1.3437176057677749	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCT	3297	1.2800453470720468	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCT	2247	0.8723875932274459	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	2094	0.8129860348100898	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGT	1557	0.6044982121295653	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGC	1403	0.5447084082323571	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATG	1213	0.470941767060477	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTAT	992	0.3851395160131848	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTT	905	0.35136215926606074	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGGTAGGAA	823	0.3195260299181967	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	681	0.26439517177921257	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	646	0.25080657998439254	No Hit
TGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTGCTT	616	0.2391592155888325	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	584	0.2267353602335685	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGGTAGGAATCG	440	0.17082801113488036	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCG	418	0.16228661057813634	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGC	359	0.1393801272668683	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	338	0.13122697218997628	No Hit
TCCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	308	0.11957960779441625	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	296	0.11492066203619225	No Hit
GCACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCT	291	0.11297943463693225	No Hit
TGCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTCTG	288	0.11181469819737623	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGT	271	0.10521452503989223	No Hit
GACTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCCGTCTTC	264	0.10249680668092821	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGGAATCGTATGCC	262	0.10172031572122421	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9772138727874861	0.0
2	0.0	0.0	0.0	5.225784158807931	0.0
3	0.0	0.0	0.0	6.265117308371737	0.0
4	0.0	0.0	0.0	9.475907426747785	0.0
5	0.0	0.0	0.0	17.3475845307471	0.0
6	0.0	0.0	0.0	18.62025321370196	0.0
7	0.0	0.0	0.0	20.762203526045447	0.0
8	0.0	0.0	0.0	23.2951170365999	0.0
9	0.0	0.0	0.0	23.786635814092534	0.0
10	0.0	0.0	0.0	32.0655824264566	0.0
11	0.0	0.0	0.0	33.91634862891109	0.0
12	0.0	0.0	0.0	44.460319370731725	0.0
13	0.0	0.0	0.0	45.48489919206116	0.0
14	0.0	0.0	0.0	46.04474917400774	0.0
15	0.0	0.0	0.0	48.25308946340592	0.0
16	0.0	0.0	0.0	49.383660300734945	0.0
17	0.0	0.0	0.0	50.21217615473912	0.0
18	0.0	0.0	0.0	50.86792277020915	0.0
19	0.0	0.0	0.0	54.56130202004123	0.0
20	0.0	0.0	0.0	55.41544207571563	0.0
21	0.0	0.0	0.0	56.03896431635795	0.0
22	0.0	0.0	0.0	56.71528794226013	0.0
23	0.0	0.0	0.0	57.224277766346106	0.0
24	0.0	0.0	0.0	57.654842003501976	0.0
25	0.0	0.0	0.0	58.056287829668946	0.0
26	0.0	0.0	0.0	58.49073452162333	0.0
27	0.0	0.0	0.0	58.87781526503578	0.0
28	0.0	0.0	0.0	59.189576385356936	0.0
29	0.0	0.0	0.0	59.50910241527513	0.0
30	0.0	0.0	0.0	59.823581253955254	0.0
31	0.0	0.0	0.0	60.17300218582205	0.0
32	0.0	0.0	0.0	60.48748102450217	0.0
33	0.0	0.0	0.0	60.786041798508364	0.0
34	0.0	0.0	0.0	61.08110836319588	0.0
35	0.0	0.0	0.0	61.395587201876005	0.0
36	0.0	0.0	0.0	61.687159557244854	0.0
37	0.0	0.0	0.0	61.9868850676906	0.0
38	3.882454798520008E-4	0.0	0.0	62.27612795018034	0.0
39	3.882454798520008E-4	0.0	0.0	62.55721767759319	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCC	20	7.023083E-4	45.000004	35
ACTGCGA	20	7.023083E-4	45.000004	30
GATGTGA	20	7.023083E-4	45.000004	10
CAAAATA	20	7.023083E-4	45.000004	37
CGCGGGT	20	7.023083E-4	45.000004	4
GGGTAAT	20	7.023083E-4	45.000004	7
TCCCTTG	20	7.023083E-4	45.000004	1
GAATCTA	20	7.023083E-4	45.000004	9
ATGCTCC	40	6.7793735E-9	45.000004	3
GGTTTCG	20	7.023083E-4	45.000004	1
CGATTTC	20	7.023083E-4	45.000004	10
CGCGATA	20	7.023083E-4	45.000004	43
TACGGGC	20	7.023083E-4	45.000004	29
AGGCGAA	20	7.023083E-4	45.000004	31
TCGGCGA	20	7.023083E-4	45.000004	30
GGGACGG	20	7.023083E-4	45.000004	7
TCTCGTG	20	7.023083E-4	45.000004	1
TAGGGTG	40	6.7793735E-9	45.000004	5
GCGATGT	20	7.023083E-4	45.000004	9
CGTACCT	20	7.023083E-4	45.000004	31
>>END_MODULE