FastQCFastQC Report
Sat 14 Jan 2017
SRR2933812.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933812.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences336202
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC280978.35717812505577No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG224416.674856187649092No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC191525.69657527319885No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC107053.1840976555761116TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT86252.5654219784534296No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT60911.8117084371895469No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA53821.6008233145549402No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC45881.3646557724225317No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT41651.2388385553922938No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT35021.0416356833094391Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC34021.0118916603708485TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC30600.910167101920869TruSeq Adapter, Index 14 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCTT20390.6064806277178602TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT19640.5841726105139172No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG17150.5101099933968269No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT13130.39053902118369316No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT11050.3286714534714249No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA10200.303389033973623No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG9200.2736450110350325No Hit
TCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG8100.240926585802583No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT7110.2114800030933784No Hit
TGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG6820.20285423644118714No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6250.18590014336619057No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC5390.16032028363900275No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCCTTGTATCG5060.1505047560692679No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC4960.14753035377540882No Hit
GCACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT4650.13830970666444578No Hit
TTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG4430.13176602161795586No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC4260.1267095377183955No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG3850.11451448831357339No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC3830.11391960785480158No Hit
GAATCTGTCTCTTATACACATCTGCCGCCCCTTGTATCGTATGCCGTCTTC3820.11362216762541569No Hit
GCCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT3810.11332472739602975No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC3810.11332472739602975No Hit
CGTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT3680.109458004414013No Hit
GGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT3650.10856568372585527No Hit
GCATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC3570.10618616189076804No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGT3460.1029143193675231No Hit
TTTCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC3430.10202199867936539No Hit
GGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG3420.10172455844997949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTG502.1827873E-1145.0000041
ACGCCGT253.884898E-545.00000425
CAAGTCA253.884898E-545.00000425
AGCCACG253.884898E-545.00000421
ATCCGCT253.884898E-545.00000410
ACGATAC253.884898E-545.0000043
GCGGGCA253.884898E-545.0000045
AACGATA253.884898E-545.0000042
CGGGATA253.884898E-545.0000046
TAGGGAG253.884898E-545.0000045
GGGCCGA253.884898E-545.0000047
GTTTTCG253.884898E-545.0000041
TTCGGGT253.884898E-545.0000044
GAACCCG253.884898E-545.0000049
CTCCTCG253.884898E-545.0000041
ATACGTC253.884898E-545.00000418
CGCCGTT253.884898E-545.00000426
TTTTCCG1100.045.0000041
TGATGGG253.884898E-545.0000043
CCCATCG253.884898E-545.00000440
Produced by FastQC (version 0.11.5)