##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933812.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	336202
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.896312336036072	25.0	16.0	31.0	16.0	33.0
2	24.6384286827562	28.0	16.0	31.0	16.0	34.0
3	25.730944491704392	30.0	16.0	33.0	16.0	34.0
4	30.496766824706576	32.0	28.0	35.0	19.0	37.0
5	33.057694481293986	35.0	32.0	35.0	28.0	37.0
6	31.025808888703814	35.0	28.0	35.0	17.0	37.0
7	33.08836056894367	35.0	32.0	35.0	28.0	37.0
8	31.60033848698104	35.0	28.0	36.0	17.0	37.0
9	34.04632334132456	35.0	32.0	38.0	28.0	39.0
10	33.696494964336914	35.0	32.0	37.0	28.0	39.0
11	34.43195757312568	35.0	32.0	39.0	27.0	39.0
12	34.44234418593584	37.0	34.0	39.0	27.0	39.0
13	34.65051070487385	35.0	33.0	38.0	29.0	39.0
14	34.32564946074086	36.0	32.0	38.0	27.0	40.0
15	35.024541793326634	37.0	33.0	39.0	27.0	40.0
16	34.33738645219243	36.0	32.0	38.0	27.0	40.0
17	33.878778234513774	36.0	32.0	38.0	27.0	40.0
18	34.279486737140175	36.0	32.0	38.0	27.0	40.0
19	33.8817199183824	35.0	32.0	39.0	27.0	40.0
20	33.778195846544634	35.0	31.0	39.0	25.0	40.0
21	33.777618812499625	36.0	32.0	39.0	25.0	40.0
22	35.183606879197626	37.0	33.0	39.0	28.0	40.0
23	35.38531894515797	37.0	33.0	39.0	29.0	40.0
24	35.6732529848127	37.0	34.0	39.0	30.0	40.0
25	33.67634041439373	36.0	32.0	39.0	24.0	40.0
26	34.86718103997002	37.0	33.0	39.0	27.0	40.0
27	34.959197149332844	37.0	33.0	39.0	27.0	40.0
28	35.13336922445435	37.0	33.0	39.0	27.0	40.0
29	35.49295066656355	38.0	34.0	40.0	27.0	40.0
30	34.04798603220683	37.0	32.0	39.0	24.0	40.0
31	34.51489580668765	37.0	33.0	39.0	25.0	40.0
32	34.86034883790102	37.0	33.0	39.0	27.0	40.0
33	35.154874153038946	37.0	34.0	39.0	27.0	40.0
34	35.52125805319421	38.0	35.0	40.0	29.0	40.0
35	35.580689585427805	38.0	34.0	40.0	30.0	40.0
36	35.70040927775563	38.0	35.0	40.0	30.0	41.0
37	36.06617152783148	38.0	35.0	40.0	30.0	41.0
38	35.71319325881464	38.0	35.0	40.0	30.0	40.0
39	35.35039946222807	37.0	34.0	40.0	29.0	40.0
40	35.09151938417975	37.0	33.0	40.0	28.0	40.0
41	34.339768948429814	37.0	33.0	39.0	25.0	40.0
42	35.182928715474624	37.0	34.0	40.0	29.0	40.0
43	35.381868638497096	38.0	35.0	40.0	30.0	40.0
44	35.98116608467529	38.0	35.0	40.0	30.0	40.0
45	36.23929661334555	38.0	35.0	40.0	31.0	41.0
46	35.906883361788445	38.0	35.0	40.0	30.0	41.0
47	35.177369557587404	37.0	34.0	40.0	28.0	41.0
48	35.5018887454566	38.0	34.0	40.0	29.0	41.0
49	35.81392734130077	38.0	35.0	40.0	30.0	41.0
50	35.514485339171095	38.0	34.0	40.0	28.0	41.0
51	35.05948804587718	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	5.0
12	13.0
13	7.0
14	12.0
15	21.0
16	50.0
17	131.0
18	341.0
19	792.0
20	1161.0
21	1491.0
22	1597.0
23	1848.0
24	2242.0
25	3030.0
26	4179.0
27	5497.0
28	7568.0
29	10214.0
30	13743.0
31	18611.0
32	25991.0
33	33728.0
34	41620.0
35	49577.0
36	50153.0
37	40820.0
38	20114.0
39	1642.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.54994913772077	2.8807086216024893	32.53371485000089	11.035627390675844
2	12.75304727515006	39.84895985151784	30.51707009476446	16.880922778567648
3	11.682559889590188	39.83022111706653	34.24221152759353	14.245007465749756
4	14.741435208594833	2.54579092331396	64.67153675468914	18.04123711340206
5	39.81296958376214	3.202538949798038	34.509312853582074	22.475178612857746
6	13.294983373091176	31.7612625742857	37.90697259385726	17.036781458765862
7	48.36318641768936	1.13235495327214	44.01044610085603	6.494012528182462
8	41.19636409063598	27.101861380955494	17.541834968263128	14.159939560145387
9	38.329040279355866	3.6272835973611106	18.7220777984664	39.32159832481663
10	38.42035442977733	16.724766658140048	26.05576409420527	18.799114817877346
11	31.659835456065107	19.497801916704837	29.013509735218708	19.82885289201135
12	18.578414167673007	14.481472448111552	33.95518170623613	32.98493167797931
13	19.658717080802614	22.57214412763755	44.97266524291943	12.7964735486404
14	18.080201783451617	32.5988542602364	23.12419319337779	26.19675076293419
15	7.900607372948406	24.0313858930048	47.12999922665541	20.93800750739139
16	8.521960012135562	26.31572685468855	22.596534226447197	42.56577890672869
17	10.036823100397974	30.416535297232024	35.57147191271914	23.975169689650865
18	9.649258481508141	24.51532114621567	34.55809305120136	31.277327321074832
19	10.324150361984758	28.767824105745948	28.593821571555196	32.314203960714096
20	13.203669222669703	26.58758722434727	45.7436303174877	14.465113235495327
21	17.69412436570871	37.24219368117977	26.731548295370043	18.332133657741476
22	11.367273246441128	21.85739525642322	35.41947995550294	31.355851541632706
23	14.53679633077733	35.42423899917312	27.671756860458892	22.367207809590663
24	21.965960940149078	21.94395036317452	22.679520050445863	33.41056864623054
25	9.966329766033516	41.13568628384126	18.700067221491842	30.197916728633384
26	12.672143532757094	19.463893730554847	29.15866056715903	38.705302169529034
27	19.336886752607064	27.755040124686943	19.845806985086348	33.062266137619645
28	8.055871172687848	22.955247143086595	34.87040529205656	34.11847639216899
29	17.84730608384245	14.445184740126471	27.030773166132267	40.67673600989881
30	12.451145442323366	26.812749477992398	31.77197042254359	28.96413465714065
31	24.100689466451716	17.04273026335358	20.93503310509753	37.921547165097174
32	19.054615974919837	28.134276417153973	32.173217291985175	20.637890315941014
33	14.875878192277261	19.821714326506086	26.553679038197274	38.748728443019374
34	28.684838281747282	17.37437611911886	26.25534648812321	27.68543911101064
35	12.093324846372122	18.847300135037866	31.313317588830525	37.74605742975949
36	15.769091201123134	24.352323900512193	21.664653987781154	38.213930910583514
37	13.311045145478015	16.009422906466945	40.97328391859656	29.706248029458482
38	15.809840512549004	20.93295102349183	29.08816723279457	34.1690412311646
39	25.957311378278536	20.324090873938882	36.4902647812922	17.228332966490385
40	16.94665706926193	16.521614981469472	44.17165870518319	22.360069244085402
41	25.48854557676635	28.81244014015384	20.19054021094461	25.508474072135208
42	16.701566320247945	15.034711274769336	44.24780340390598	24.015919001076732
43	22.747633862975235	24.405268261342883	22.404090398034516	30.443007477647367
44	20.492144603541917	14.753035377540883	33.348403638288886	31.40641638062831
45	16.69264311336637	11.658467231009928	29.224989738312086	42.423899917311616
46	31.434970642649358	20.74615855943748	29.285667545106808	18.53320325280635
47	10.518676272003141	14.070707491329618	53.419372877020365	21.991243359646877
48	22.493917347309058	21.32884396880447	25.84963801524084	30.327600668645637
49	16.760459485666352	10.460377987043504	49.019042123485285	23.760120403804855
50	25.47991981011416	13.995157673065597	32.03639478646766	28.488527730352587
51	18.98471752101415	12.15370521293746	27.579847829578647	41.281729436469746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	76.0
2	109.0
3	566.0
4	1023.0
5	745.0
6	467.0
7	665.0
8	863.0
9	1091.5
10	1320.0
11	1461.5
12	1603.0
13	1498.0
14	1393.0
15	1258.5
16	1124.0
17	1065.0
18	1006.0
19	1013.5
20	1021.0
21	1127.0
22	1233.0
23	1225.0
24	1217.0
25	1299.5
26	1627.0
27	1872.0
28	1776.5
29	1681.0
30	2044.0
31	2407.0
32	2499.0
33	2591.0
34	2769.0
35	2947.0
36	3502.5
37	4058.0
38	4523.0
39	4988.0
40	6082.5
41	7177.0
42	8497.5
43	9818.0
44	12944.5
45	16071.0
46	68562.0
47	121053.0
48	88765.0
49	56477.0
50	50383.5
51	44290.0
52	30313.5
53	16337.0
54	13024.0
55	9711.0
56	8135.5
57	6560.0
58	5840.0
59	5120.0
60	4251.0
61	3382.0
62	2845.5
63	2309.0
64	1819.5
65	1330.0
66	1129.5
67	929.0
68	722.5
69	516.0
70	423.5
71	331.0
72	271.0
73	211.0
74	180.5
75	106.0
76	62.0
77	35.5
78	9.0
79	6.5
80	4.0
81	3.0
82	2.0
83	3.0
84	4.0
85	2.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	336202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.06855997287345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.68705876769378	18.722970119154557
2	10.784162504004462	5.406868489776979
3	4.053108055196308	3.0481674707467534
4	2.152323774041599	2.158226304424126
5	1.3704156334167843	1.7177173247036006
6	0.9527651546611929	1.4330670251812898
7	0.7119042251515763	1.2492489634208006
8	0.6774955209359167	1.3587069678348136
9	0.5647773519535839	1.2742339426892166
>10	3.7730923933033544	15.574565291104753
>50	0.12695625348536443	2.2409146881934077
>100	0.11627769010809079	6.208469907971994
>500	0.008305549293435056	1.4256310194466422
>1k	0.01305157746111223	8.290551513673329
>5k	0.0035595211257578815	5.977953730197917
>10k+	0.004746028167677175	23.912707241479826
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	28097	8.35717812505577	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	22441	6.674856187649092	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	19152	5.69657527319885	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	10705	3.1840976555761116	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	8625	2.5654219784534296	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	6091	1.8117084371895469	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	5382	1.6008233145549402	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	4588	1.3646557724225317	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	4165	1.2388385553922938	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	3502	1.0416356833094391	Illumina Paired End PCR Primer 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	3402	1.0118916603708485	TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	3060	0.910167101920869	TruSeq Adapter, Index 14 (95% over 21bp)
TGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCTT	2039	0.6064806277178602	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	1964	0.5841726105139172	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	1715	0.5101099933968269	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	1313	0.39053902118369316	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	1105	0.3286714534714249	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	1020	0.303389033973623	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	920	0.2736450110350325	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	810	0.240926585802583	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	711	0.2114800030933784	No Hit
TGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	682	0.20285423644118714	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	625	0.18590014336619057	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	539	0.16032028363900275	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCCTTGTATCG	506	0.1505047560692679	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	496	0.14753035377540882	No Hit
GCACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	465	0.13830970666444578	No Hit
TTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	443	0.13176602161795586	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	426	0.1267095377183955	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	385	0.11451448831357339	No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	383	0.11391960785480158	No Hit
GAATCTGTCTCTTATACACATCTGCCGCCCCTTGTATCGTATGCCGTCTTC	382	0.11362216762541569	No Hit
GCCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	381	0.11332472739602975	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	381	0.11332472739602975	No Hit
CGTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	368	0.109458004414013	No Hit
GGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	365	0.10856568372585527	No Hit
GCATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	357	0.10618616189076804	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGT	346	0.1029143193675231	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	343	0.10202199867936539	No Hit
GGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	342	0.10172455844997949	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.1844069934146733	0.0
2	0.0	0.0	0.0	6.666825301455672	0.0
3	0.0	0.0	0.0	8.093646081819859	0.0
4	0.0	0.0	0.0	11.95769210177215	0.0
5	0.0	0.0	0.0	20.75567664677783	0.0
6	0.0	0.0	0.0	22.3770233371604	0.0
7	0.0	0.0	0.0	24.80562281009631	0.0
8	0.0	0.0	0.0	27.909411603738228	0.0
9	0.0	0.0	0.0	28.538497688889418	0.0
10	0.0	0.0	0.0	36.87188059559431	0.0
11	0.0	0.0	0.0	39.18358605838157	0.0
12	0.0	0.0	0.0	50.260557640942054	0.0
13	0.0	0.0	0.0	51.4306875033462	0.0
14	0.0	0.0	0.0	52.05055294138643	0.0
15	0.0	0.0	0.0	54.14007055282241	0.0
16	0.0	0.0	0.0	55.47795670460021	0.0
17	0.0	0.0	0.0	56.60019869007323	0.0
18	0.0	0.0	0.0	57.61179291021469	0.0
19	0.0	0.0	0.0	61.19951695706748	0.0
20	0.0	0.0	0.0	62.17541834968263	0.0
21	0.0	0.0	0.0	62.97969672994212	0.0
22	0.0	0.0	0.0	63.93358754558272	0.0
23	0.0	0.0	0.0	64.62840792142819	0.0
24	0.0	0.0	0.0	65.22031397790614	0.0
25	0.0	0.0	0.0	65.69205418171218	0.0
26	0.0	0.0	0.0	66.17598943492305	0.0
27	0.0	0.0	0.0	66.65754516629883	0.0
28	0.0	0.0	0.0	67.06355107941059	0.0
29	0.0	0.0	0.0	67.45081825807104	0.0
30	0.0	0.0	0.0	67.89816836306744	0.0
31	0.0	0.0	0.0	68.35176471288095	0.0
32	0.0	0.0	0.0	68.74498069612912	0.0
33	0.0	0.0	0.0	69.1566379735992	0.0
34	2.974402293859049E-4	0.0	0.0	69.50523792243949	0.0
35	2.974402293859049E-4	0.0	0.0	69.85056602875652	0.0
36	2.974402293859049E-4	0.0	0.0	70.19827365690864	0.0
37	2.974402293859049E-4	0.0	0.0	70.52248350693928	0.0
38	2.974402293859049E-4	0.0	0.0	70.88446826610193	0.0
39	2.974402293859049E-4	0.0	0.0	71.23990934021808	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTG	50	2.1827873E-11	45.000004	1
ACGCCGT	25	3.884898E-5	45.000004	25
CAAGTCA	25	3.884898E-5	45.000004	25
AGCCACG	25	3.884898E-5	45.000004	21
ATCCGCT	25	3.884898E-5	45.000004	10
ACGATAC	25	3.884898E-5	45.000004	3
GCGGGCA	25	3.884898E-5	45.000004	5
AACGATA	25	3.884898E-5	45.000004	2
CGGGATA	25	3.884898E-5	45.000004	6
TAGGGAG	25	3.884898E-5	45.000004	5
GGGCCGA	25	3.884898E-5	45.000004	7
GTTTTCG	25	3.884898E-5	45.000004	1
TTCGGGT	25	3.884898E-5	45.000004	4
GAACCCG	25	3.884898E-5	45.000004	9
CTCCTCG	25	3.884898E-5	45.000004	1
ATACGTC	25	3.884898E-5	45.000004	18
CGCCGTT	25	3.884898E-5	45.000004	26
TTTTCCG	110	0.0	45.000004	1
TGATGGG	25	3.884898E-5	45.000004	3
CCCATCG	25	3.884898E-5	45.000004	40
>>END_MODULE