SRR2933811.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933811.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262124
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	24136	9.207855823961179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	20644	7.875661900474585	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	15418	5.881949001236056	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	8713	3.323999328562054	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT	7608	2.902443118524057	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA	4480	1.7091147701088036	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	4220	1.6099250736292747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC	3639	1.3882742518807893	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	3538	1.3497428697868183	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2361	0.900718743800644	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	2043	0.779402115029528	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2032	0.7752056278707787	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGT	1595	0.6084906380186477	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATG	1401	0.5344798644916147	No Hit
TGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCTT	1139	0.4345271703468588	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTAT	1050	0.40057377424425084	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT	989	0.37730234545482294	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTCAAA	902	0.34411194701744213	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	644	0.2456852482031405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.22851780073552974	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	485	0.1850269338175825	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	451	0.17205597350872107	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCCTCAAATCG	448	0.17091147701088033	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	446	0.17014847934565322	No Hit
TGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	434	0.16557049335429033	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	407	0.15527002487372388	No Hit
GCACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	351	0.13390609024736383	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	333	0.12703911126031955	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	318	0.12131662877111597	No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	313	0.11940913460804808	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	303	0.11559414628191236	No Hit
TTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	277	0.10567517663395949	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	272	0.10376768247089163	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGT	272	0.10376768247089163	No Hit
GCATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	269	0.10262318597305092	No Hit
GAATCTGTCTCTTATACACATCTGCCGCCCCTCAAATCGTATGCCGTCTTC	265	0.10109719064259662	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	30	2.1590677E-6	45.000004	1
AACGTCC	20	7.0233113E-4	45.000004	21
CGGCGCC	40	6.7811925E-9	45.000004	31
CTCACCA	20	7.0233113E-4	45.000004	28
TCTTGCG	60	0.0	45.000004	1
ACCGGTG	60	0.0	45.000004	17
ACCGGTC	20	7.0233113E-4	45.000004	17
ACTGCGA	25	3.8825397E-5	45.000004	30
CGAGTTG	20	7.0233113E-4	45.000004	20
TTGTGCG	25	3.8825397E-5	45.000004	1
CAACGTC	20	7.0233113E-4	45.000004	20
CACGGGA	25	3.8825397E-5	45.000004	4
TCTCTCG	25	3.8825397E-5	45.000004	1
GCGAACA	20	7.0233113E-4	45.000004	33
GAGTCAT	20	7.0233113E-4	45.000004	9
TTTCGTG	30	2.1590677E-6	45.000004	1
TTGCTAG	20	7.0233113E-4	45.000004	1
TATGGGT	20	7.0233113E-4	45.000004	4
CGTGAAT	30	2.1590677E-6	45.000004	19
GGGTAAT	25	3.8825397E-5	45.000004	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content