##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933811.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262124
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.3455654575697	25.0	16.0	31.0	16.0	33.0
2	24.052498054355954	25.0	16.0	31.0	16.0	34.0
3	25.176740779173215	28.0	16.0	31.0	16.0	34.0
4	30.156570935892937	32.0	28.0	35.0	19.0	37.0
5	32.920217149135524	35.0	32.0	35.0	28.0	37.0
6	30.58000793517572	35.0	28.0	35.0	17.0	37.0
7	32.926935343577846	33.0	32.0	35.0	28.0	37.0
8	31.118543132258015	35.0	28.0	35.0	17.0	37.0
9	33.90037920983962	34.0	32.0	37.0	28.0	39.0
10	33.57316766110696	35.0	32.0	37.0	28.0	39.0
11	34.36355694251576	35.0	32.0	38.0	27.0	39.0
12	34.509583250675256	35.0	34.0	39.0	27.0	39.0
13	34.59738902198959	35.0	33.0	38.0	29.0	39.0
14	34.450630236071476	36.0	32.0	38.0	27.0	40.0
15	35.040282461735664	37.0	33.0	39.0	27.0	40.0
16	34.45888587080923	36.0	32.0	38.0	27.0	40.0
17	33.89900581404221	36.0	32.0	38.0	27.0	40.0
18	34.31501121606568	36.0	32.0	38.0	27.0	40.0
19	33.92770597121973	35.0	32.0	39.0	27.0	40.0
20	33.4893294776518	35.0	31.0	38.0	25.0	40.0
21	33.73851688513833	36.0	32.0	38.0	25.0	40.0
22	35.14149028703972	37.0	33.0	39.0	28.0	40.0
23	35.17354381895591	37.0	33.0	39.0	27.0	40.0
24	35.50634051059804	37.0	34.0	39.0	29.0	40.0
25	33.625921319680764	36.0	32.0	39.0	24.0	40.0
26	34.790011597564515	37.0	33.0	39.0	27.0	40.0
27	34.746207901603825	37.0	33.0	39.0	27.0	40.0
28	34.84379530298637	37.0	33.0	39.0	27.0	40.0
29	35.21839282171796	37.0	33.0	40.0	27.0	40.0
30	33.775220887824084	36.0	32.0	39.0	24.0	40.0
31	34.165951992186905	36.0	32.0	39.0	25.0	40.0
32	34.37161038287223	36.0	33.0	39.0	26.0	40.0
33	34.561978300346404	37.0	33.0	39.0	25.0	40.0
34	34.84646579481467	37.0	34.0	39.0	25.0	40.0
35	34.88042682089393	37.0	33.0	40.0	25.0	40.0
36	35.08645145045856	38.0	34.0	40.0	26.0	40.0
37	35.552417939601106	38.0	35.0	40.0	30.0	40.0
38	35.43301643496971	38.0	35.0	40.0	30.0	40.0
39	34.97717873983306	37.0	34.0	39.0	28.0	40.0
40	34.73395415910027	37.0	33.0	39.0	27.0	40.0
41	33.75497474477728	36.0	32.0	39.0	24.0	40.0
42	34.75289939112786	37.0	33.0	39.0	27.0	40.0
43	35.153351085745676	37.0	34.0	40.0	29.0	40.0
44	35.778417848041386	38.0	35.0	40.0	30.0	40.0
45	36.01511116875982	38.0	35.0	40.0	31.0	40.0
46	35.67587096183485	38.0	35.0	40.0	30.0	40.0
47	34.737380018617145	37.0	33.0	40.0	26.0	41.0
48	35.23388167432208	37.0	34.0	40.0	28.0	41.0
49	35.67391387282355	38.0	35.0	40.0	29.0	41.0
50	35.40549510918497	38.0	34.0	40.0	28.0	41.0
51	34.99390746364316	37.0	34.0	40.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	7.0
13	7.0
14	6.0
15	14.0
16	33.0
17	136.0
18	298.0
19	656.0
20	1112.0
21	1396.0
22	1497.0
23	1703.0
24	1841.0
25	2493.0
26	3316.0
27	4663.0
28	6255.0
29	8604.0
30	11856.0
31	16031.0
32	21697.0
33	27895.0
34	34271.0
35	38571.0
36	35687.0
37	27655.0
38	13245.0
39	1172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	57.33431505699592	3.0477941737498284	27.87077871541713	11.747112053837116
2	12.654697776624804	43.76134959027026	26.40734919351147	17.176603439593475
3	10.582014619035265	43.977277929529535	32.44647571378432	12.994231737650882
4	14.453846271230411	2.5270482672323022	66.87331186766569	16.145793593871602
5	43.10974958416627	3.0638171247195984	33.08777525140773	20.738658039706397
6	13.481405746898414	35.52822328363675	34.69693732737178	16.293433642093056
7	45.54981611756268	1.157467458149578	47.09336039431719	6.199356029970549
8	38.08083197265416	30.93688483313241	17.521859883108757	13.46042331110467
9	35.91582609757214	3.367871694312615	17.650806488532144	43.0654957195831
10	39.68350856846378	14.28942027437396	25.676778929056475	20.350292228105783
11	33.22435183348339	18.02925333048481	28.886710106667074	19.85968472936473
12	20.782911904289573	13.2139750652363	32.3587309822832	33.644382048190934
13	20.709664128427768	20.568509560360745	46.43451191039355	12.287314400817934
14	18.793395492209793	31.73574338862523	22.15287421220491	27.317986906960062
15	7.4010773527033	22.26579786665853	49.469716622667136	20.863408157971037
16	8.386107338511545	24.889365338542063	22.314629717233064	44.409897605713326
17	10.05707222535899	29.600112923654454	37.17820573469045	23.164609116296102
18	9.017106407654392	23.546107948909675	35.260792601974636	32.17599304146129
19	9.776289084555401	28.14011689124231	29.04655811753216	33.03703590667013
20	13.131189818559156	25.332666982039036	47.65607117242221	13.880072026979597
21	17.923196655018238	38.45775281927637	26.11741008072515	17.50164044498024
22	11.077581602600297	21.159832750911782	36.23056263447834	31.532023012009585
23	14.036486548351162	36.16990432009278	27.250843112420075	22.542766019135982
24	21.647388258991928	21.09535944820009	22.06932596786254	35.187926324945444
25	9.885397750682882	42.99224794372129	18.011322885351973	29.111031420243854
26	12.239627046741237	18.729685187163327	30.517617616090092	38.51307015000534
27	19.46902992476843	27.517892295249574	19.810089881124966	33.20298789885703
28	7.949291175169004	23.117303261052022	35.20929025957181	33.72411530420717
29	18.541224763852224	14.174589125757276	26.223466756191726	41.060719354198774
30	10.757122583204895	29.77255039599579	25.807633028642936	33.662693992156385
31	19.07723062367429	22.696128549846637	17.47417252903206	40.75246829744701
32	19.187865285132226	34.97924646350582	22.376432528116464	23.456455723245487
33	11.221406662495612	23.29012223222597	15.493812088935007	49.99465901634341
34	20.949626894141705	29.07097404281943	20.2327905876608	29.746608475378068
35	10.502662861851643	27.92724054264394	19.56821962124796	42.00187697425646
36	13.268147899467428	28.064580122384825	15.615891715371353	43.0513802627764
37	9.934611100090034	20.884009094932168	38.918984907906186	30.262394897071616
38	15.060429415085991	23.645679144221816	16.074453312172864	45.21943812851933
39	16.952282126016694	30.497779676794188	33.45286963421892	19.0970685629702
40	14.069295447955929	26.987227419084096	24.063801864766294	34.87967526819368
41	14.277593810562939	43.56373319497642	15.855091483420061	26.303581511040576
42	14.595763836962657	27.426332575422318	33.45172513772108	24.526178449893944
43	21.95487631807847	26.969678472783876	17.914803680700736	33.160641528436926
44	19.030306267262823	16.99119500694328	32.167981565976405	31.810517159817493
45	14.69647952877264	14.755611847827746	26.546596267415424	44.00131235598419
46	30.36349208771421	24.523126459233037	26.335245914147503	18.778135538905254
47	9.208237322793792	15.674261036761228	49.95460163891898	25.162900001525994
48	20.08858402893287	27.316842410462222	21.764889899436906	30.829683661167994
49	15.276739253177885	15.778028719232118	43.9959713723276	24.949260655262396
50	25.280783140803585	16.34722497749157	28.63301338297905	29.738978498725793
51	18.795684485205474	13.625993804458957	25.385313820939707	42.19300788939586
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	79.5
2	98.0
3	526.5
4	955.0
5	679.5
6	404.0
7	502.5
8	601.0
9	818.0
10	1035.0
11	1021.0
12	1007.0
13	933.5
14	860.0
15	809.0
16	758.0
17	730.0
18	702.0
19	706.0
20	710.0
21	751.5
22	793.0
23	830.0
24	867.0
25	978.5
26	1096.0
27	1102.0
28	1254.0
29	1406.0
30	1643.5
31	1881.0
32	1902.5
33	1924.0
34	2177.5
35	2431.0
36	2602.0
37	2773.0
38	3251.5
39	3730.0
40	4159.0
41	4588.0
42	5693.0
43	6798.0
44	9142.0
45	11486.0
46	54611.0
47	97736.0
48	70238.0
49	42740.0
50	38259.0
51	33778.0
52	23696.5
53	13615.0
54	10845.5
55	8076.0
56	6778.0
57	5480.0
58	4760.0
59	4040.0
60	3698.0
61	3356.0
62	2744.0
63	2132.0
64	1687.5
65	1243.0
66	926.5
67	610.0
68	558.0
69	506.0
70	422.0
71	338.0
72	271.5
73	205.0
74	156.5
75	80.0
76	52.0
77	29.5
78	7.0
79	11.5
80	16.0
81	10.0
82	4.0
83	10.0
84	16.0
85	8.0
86	0.0
87	2.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262124.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.034243335215397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.41543331086427	19.633837420457493
2	10.38656545822221	5.4081274511299995
3	4.047360862811758	3.1610993270360592
4	2.0969490929339702	2.1836993178800874
5	1.4389965124117112	1.8731592681326397
6	1.0272266346238388	1.6045840899726846
7	0.8425896075730488	1.535532801269628
8	0.725359749128103	1.5107353771497458
9	0.5788224260719206	1.356228349941249
>10	3.18132528354972	12.969434313531
>50	0.13041821752000235	2.384367703834826
>100	0.09964537967820403	5.5012131662877115
>500	0.005861492922247297	1.1956173414109352
>1k	0.016119105536180067	10.490454899208009
>5k	0.0029307464611236485	6.226442447086112
>10k+	0.004396119691685472	22.965466725671817
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	24136	9.207855823961179	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	20644	7.875661900474585	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	15418	5.881949001236056	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	8713	3.323999328562054	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTCAAAT	7608	2.902443118524057	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTA	4480	1.7091147701088036	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	4220	1.6099250736292747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTC	3639	1.3882742518807893	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCT	3538	1.3497428697868183	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2361	0.900718743800644	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	2043	0.779402115029528	No Hit
TCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	2032	0.7752056278707787	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGT	1595	0.6084906380186477	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATG	1401	0.5344798644916147	No Hit
TGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCTT	1139	0.4345271703468588	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTAT	1050	0.40057377424425084	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTT	989	0.37730234545482294	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTCAAA	902	0.34411194701744213	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	644	0.2456852482031405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	599	0.22851780073552974	No Hit
TCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	485	0.1850269338175825	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	451	0.17205597350872107	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCCCTCAAATCG	448	0.17091147701088033	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	446	0.17014847934565322	No Hit
TGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	434	0.16557049335429033	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGC	407	0.15527002487372388	No Hit
GCACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCT	351	0.13390609024736383	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCC	333	0.12703911126031955	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	318	0.12131662877111597	No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	313	0.11940913460804808	No Hit
GTTCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	303	0.11559414628191236	No Hit
TTCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTG	277	0.10567517663395949	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	272	0.10376768247089163	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGT	272	0.10376768247089163	No Hit
GCATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	269	0.10262318597305092	No Hit
GAATCTGTCTCTTATACACATCTGCCGCCCCTCAAATCGTATGCCGTCTTC	265	0.10109719064259662	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.814988326135722E-4	0.0	0.0	0.958706566357907	0.0
2	3.814988326135722E-4	0.0	0.0	6.359967038500862	0.0
3	3.814988326135722E-4	0.0	0.0	7.600219743327585	0.0
4	3.814988326135722E-4	0.0	0.0	10.903236636095894	0.0
5	3.814988326135722E-4	0.0	0.0	19.636889411118403	0.0
6	3.814988326135722E-4	0.0	0.0	21.09535944820009	0.0
7	3.814988326135722E-4	0.0	0.0	23.59036181349285	0.0
8	3.814988326135722E-4	0.0	0.0	26.494330927347363	0.0
9	3.814988326135722E-4	0.0	0.0	27.09519158871374	0.0
10	3.814988326135722E-4	0.0	0.0	36.76847598846348	0.0
11	3.814988326135722E-4	0.0	0.0	38.925088889228	0.0
12	3.814988326135722E-4	0.0	0.0	50.82937846210191	0.0
13	3.814988326135722E-4	0.0	0.0	51.96395599029467	0.0
14	3.814988326135722E-4	0.0	0.0	52.60525552791808	0.0
15	3.814988326135722E-4	0.0	0.0	54.87479208313623	0.0
16	3.814988326135722E-4	0.0	0.0	56.109703804306356	0.0
17	3.814988326135722E-4	0.0	0.0	57.12754268971937	0.0
18	3.814988326135722E-4	0.0	0.0	57.941661198516734	0.0
19	3.814988326135722E-4	0.0	0.0	61.909249057697885	0.0
20	3.814988326135722E-4	0.0	0.0	62.84659168942943	0.0
21	3.814988326135722E-4	0.0	0.0	63.58936991652806	0.0
22	3.814988326135722E-4	0.0	0.0	64.40959240664723	0.0
23	3.814988326135722E-4	0.0	0.0	65.026857517816	0.0
24	3.814988326135722E-4	0.0	0.0	65.58727930292534	0.0
25	3.814988326135722E-4	0.0	0.0	66.02333246860265	0.0
26	3.814988326135722E-4	0.0	0.0	66.50363949886314	0.0
27	3.814988326135722E-4	0.0	0.0	66.95151912835146	0.0
28	3.814988326135722E-4	0.0	0.0	67.32576948314538	0.0
29	3.814988326135722E-4	0.0	0.0	67.77250461613588	0.0
30	3.814988326135722E-4	0.0	0.0	68.20092780516092	0.0
31	3.814988326135722E-4	0.0	0.0	68.59997558407471	0.0
32	3.814988326135722E-4	0.0	0.0	68.93874654743557	0.0
33	3.814988326135722E-4	0.0	0.0	69.31757488822085	0.0
34	3.814988326135722E-4	0.0	0.0	69.66016083990783	0.0
35	3.814988326135722E-4	0.0	0.0	70.030214707543	0.0
36	3.814988326135722E-4	0.0	0.0	70.37165616273214	0.0
37	3.814988326135722E-4	0.0	0.0	70.7024156506081	0.0
38	3.814988326135722E-4	0.0	0.0	71.05759106377134	0.0
39	3.814988326135722E-4	0.0	0.0	71.41848895942378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTG	30	2.1590677E-6	45.000004	1
AACGTCC	20	7.0233113E-4	45.000004	21
CGGCGCC	40	6.7811925E-9	45.000004	31
CTCACCA	20	7.0233113E-4	45.000004	28
TCTTGCG	60	0.0	45.000004	1
ACCGGTG	60	0.0	45.000004	17
ACCGGTC	20	7.0233113E-4	45.000004	17
ACTGCGA	25	3.8825397E-5	45.000004	30
CGAGTTG	20	7.0233113E-4	45.000004	20
TTGTGCG	25	3.8825397E-5	45.000004	1
CAACGTC	20	7.0233113E-4	45.000004	20
CACGGGA	25	3.8825397E-5	45.000004	4
TCTCTCG	25	3.8825397E-5	45.000004	1
GCGAACA	20	7.0233113E-4	45.000004	33
GAGTCAT	20	7.0233113E-4	45.000004	9
TTTCGTG	30	2.1590677E-6	45.000004	1
TTGCTAG	20	7.0233113E-4	45.000004	1
TATGGGT	20	7.0233113E-4	45.000004	4
CGTGAAT	30	2.1590677E-6	45.000004	19
GGGTAAT	25	3.8825397E-5	45.000004	7
>>END_MODULE