SRR2933810.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933810.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426230
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	28903	6.781080637214649	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	23652	5.549116674096146	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	18575	4.357975740797222	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCT	9915	2.3262088543743986	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	9401	2.205616685826901	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTA	5775	1.3549022828050583	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	5478	1.2852215939750837	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	4680	1.097998733078385	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	4271	1.002041151490979	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3343	0.7843183257865473	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2699	0.6332261924313164	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAGACTCTCGT	2176	0.5105224878586678	No Hit
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2131	0.49996480773291413	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATG	1824	0.4279379677638834	No Hit
TGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCTT	1658	0.3889918588555475	Illumina PCR Primer Index 8 (96% over 25bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT	1395	0.3272880838983647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.31978040025338433	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1222	0.2866996691926894	RNA PCR Primer, Index 40 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAGACTC	1113	0.2611266217769749	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1057	0.24798817539825913	RNA PCR Primer, Index 40 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	702	0.16469980996175773	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACAGACTCTCG	649	0.15226520892475892	No Hit
TGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	614	0.1440536799380616	TruSeq Adapter, Index 19 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAGCCTCTCGTATGCCGTCTTCTGC	567	0.13302676958449663	Illumina PCR Primer Index 3 (95% over 23bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	562	0.13185369401496844	No Hit
TTCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	553	0.1297421579898177	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	545	0.1278652370785726	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	473	0.11097294887736668	No Hit
TTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	444	0.10416911057410318	TruSeq Adapter, Index 19 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.1622764E-6	45.000004	34
ACTGACG	30	2.1622764E-6	45.000004	43
TTTCGCG	70	0.0	45.000004	1
GGCAATT	30	2.1622764E-6	45.000004	8
CGACCAG	35	1.2095188E-7	45.000004	34
CGTTCCC	30	2.1622764E-6	45.000004	34
TGTTGCG	30	2.1622764E-6	45.000004	1
AAACCGA	30	2.1622764E-6	45.000004	30
CGGGAAC	30	2.1622764E-6	45.000004	6
TATACGG	30	2.1622764E-6	45.000004	27
GTTATTC	30	2.1622764E-6	45.000004	42
CCACCGG	120	0.0	45.000004	15
TGCGTTC	30	2.1622764E-6	45.000004	32
CGGGTAC	25	3.8866623E-5	45.0	6
CTCCGGG	45	3.8380676E-10	45.0	3
TTTGTCG	110	0.0	45.0	1
TTGCTAG	20	7.0282863E-4	45.0	2
CGCGCAA	20	7.0282863E-4	45.0	41
CGACACT	20	7.0282863E-4	45.0	12
CGACACC	20	7.0282863E-4	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content