##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933810.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	426230
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.63150177134411	28.0	16.0	31.0	16.0	33.0
2	25.529432466039463	28.0	16.0	31.0	16.0	34.0
3	26.593719353400747	30.0	16.0	33.0	16.0	34.0
4	31.063540811299063	33.0	28.0	35.0	19.0	37.0
5	33.23156042512259	35.0	32.0	35.0	28.0	37.0
6	31.38930858925932	35.0	28.0	36.0	17.0	37.0
7	33.15207282453136	35.0	32.0	35.0	28.0	37.0
8	31.97282453135631	35.0	31.0	37.0	17.0	37.0
9	34.13345611524294	35.0	32.0	38.0	28.0	39.0
10	33.56392323393473	35.0	32.0	37.0	27.0	39.0
11	34.25486005208455	35.0	32.0	39.0	27.0	39.0
12	34.425178894024356	37.0	33.0	39.0	27.0	39.0
13	34.64402083382212	35.0	33.0	38.0	29.0	39.0
14	34.5488914435868	36.0	33.0	39.0	27.0	40.0
15	35.111507871337075	37.0	33.0	39.0	27.0	40.0
16	34.65037421110668	36.0	32.0	39.0	27.0	40.0
17	34.110907256645476	36.0	32.0	38.0	27.0	40.0
18	34.44056260704315	36.0	32.0	39.0	27.0	40.0
19	34.14743682988058	36.0	32.0	39.0	27.0	40.0
20	34.04701217652441	36.0	32.0	39.0	25.0	40.0
21	33.95213382446097	36.0	32.0	39.0	25.0	40.0
22	35.20288107359876	37.0	33.0	39.0	28.0	40.0
23	35.541102221805126	37.0	34.0	39.0	29.0	40.0
24	35.84949909673181	38.0	34.0	39.0	30.0	40.0
25	33.84559040893414	36.0	32.0	39.0	24.0	40.0
26	34.83155104051803	37.0	33.0	39.0	27.0	40.0
27	35.00652230016658	37.0	33.0	39.0	27.0	40.0
28	34.9890129742158	37.0	33.0	39.0	27.0	40.0
29	35.513366023039204	38.0	34.0	40.0	29.0	40.0
30	33.244023179973254	36.0	31.0	39.0	22.0	40.0
31	34.146237946648526	36.0	32.0	39.0	25.0	40.0
32	34.5289843511719	36.0	33.0	39.0	26.0	40.0
33	34.86365577270488	37.0	33.0	39.0	27.0	40.0
34	35.200922037397646	38.0	34.0	39.0	27.0	40.0
35	35.4591816624827	38.0	34.0	40.0	29.0	40.0
36	35.64099429885273	38.0	35.0	40.0	30.0	41.0
37	35.927306853107474	38.0	35.0	40.0	30.0	41.0
38	34.98455763320273	37.0	34.0	40.0	27.0	40.0
39	35.1477864064003	37.0	34.0	40.0	28.0	40.0
40	34.406501184806324	37.0	33.0	39.0	25.0	40.0
41	34.050756633742346	36.0	33.0	39.0	25.0	40.0
42	35.11514909790489	37.0	34.0	39.0	29.0	40.0
43	35.288351359594586	38.0	34.0	40.0	29.0	40.0
44	35.888548436290264	38.0	35.0	40.0	30.0	41.0
45	36.09145062524928	38.0	35.0	40.0	31.0	41.0
46	35.72158459047932	38.0	35.0	40.0	30.0	41.0
47	35.22045139947915	38.0	34.0	40.0	26.0	41.0
48	35.54055322243859	38.0	35.0	40.0	28.0	41.0
49	35.884649133097156	38.0	35.0	40.0	30.0	41.0
50	35.54075968373883	38.0	35.0	40.0	28.0	41.0
51	34.9959270816226	37.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	5.0
11	8.0
12	10.0
13	9.0
14	14.0
15	20.0
16	67.0
17	177.0
18	520.0
19	1165.0
20	1770.0
21	2195.0
22	2301.0
23	2471.0
24	2981.0
25	3771.0
26	5279.0
27	6799.0
28	9183.0
29	12483.0
30	17381.0
31	23940.0
32	32009.0
33	41845.0
34	51547.0
35	60470.0
36	62164.0
37	54134.0
38	28557.0
39	2950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.861647467329846	3.541749759519508	34.90580203176689	12.69080074138376
2	14.876709757642587	34.02787227553199	33.80334561152429	17.292072355301126
3	13.875841681721138	34.25849893250123	36.76606526992469	15.099594115852943
4	15.017713441099875	3.088473359453816	63.128827159045585	18.76498604040072
5	37.62334889613589	3.7951340825375968	36.45895408582221	22.122562935504305
6	15.470755226051663	28.654482321751168	39.570185111324875	16.3045773408723
7	53.898833962883884	1.5970250803556765	38.594186237477416	5.909954719283016
8	49.1384933017385	24.197499002885767	15.670178072871455	10.993829622504283
9	45.59674354221899	3.8448724866855923	16.362761889120897	34.19562208197452
10	39.130516387865704	18.07380991483471	23.975787720244938	18.819885977054643
11	32.329024235741265	19.36278535063229	28.47570560495507	19.832484808671374
12	21.67538652840016	14.913075100297963	32.240339722684936	31.171198648616944
13	23.04155033667269	19.964338502686342	42.16596673157685	14.82814442906412
14	19.38249302020036	29.82474250991249	25.355324590010085	25.43743987987706
15	11.08486028669967	22.50686249208174	44.8605213147831	21.54775590643549
16	12.762592966238884	24.23761818736363	24.643971564648197	38.35581728174929
17	13.098561809351757	28.444971025033432	35.848250944325834	22.608216221288977
18	12.237993571545879	23.60345353447669	34.35609881988598	29.802454074091457
19	13.182319405016072	27.690448818712905	29.08500105576801	30.042230720503017
20	16.319827323276165	25.93224315510405	42.63965464655233	15.108274875067451
21	19.528658236163572	34.91542125143702	27.631560425122586	17.92436008727682
22	13.608145836754804	21.79902869342843	35.953593130469464	28.6392323393473
23	16.466696384581095	32.76869295920043	28.97590502780189	21.788705628416583
24	21.61978274640452	22.66804307533491	24.75682143443681	30.955352743823756
25	12.837200572460878	38.54843629026582	21.208033221500127	27.406329915773174
26	14.273514299791193	24.846679023062666	30.649414635290807	30.230392041855335
27	20.246111254487015	29.18236632803885	22.381343406142225	28.19017901133191
28	11.806301761959505	31.612275062759544	32.746169908265486	23.83525326701546
29	22.642704643033102	25.16247096637965	23.52603054688783	28.668793843699415
30	14.6732984538864	34.51352556131666	26.247565868193227	24.56561011660371
31	21.934870844379795	28.018675363066887	18.827159045585717	31.219294746967602
32	26.164042887642825	33.810853295169274	20.64308002721535	19.38202378997255
33	15.493043661872699	25.738920301245805	18.514417098749504	40.25361893813199
34	22.229782042559183	28.70234380498792	23.274757759894893	25.79311639255801
35	15.084813363676888	21.688055744551065	26.893226661661547	36.333904230110505
36	14.387068014921523	37.28761466813692	19.580742791450625	28.744574525490936
37	19.546019754592592	20.57175703258804	30.304530417849517	29.577692794969852
38	18.789386012246908	36.083804518687096	19.687492668277688	25.43931680078831
39	27.08115336789996	23.91009548835136	23.265607770452572	25.743143373296107
40	16.483823287896207	27.734556460127163	31.76125566008962	24.02036459188701
41	15.891185510170565	24.723506088262205	20.57105318724632	38.81425521432091
42	17.64258733547615	17.95462543697065	39.10001642305797	25.30277080449523
43	21.59725969546958	21.97921310090796	21.100579499331346	35.32294770429111
44	19.887853975553107	18.267367383806864	30.387349553058208	31.457429087581822
45	17.483049058020317	18.58175163644042	27.721418013748444	36.213781291790816
46	30.836168265959696	23.22595781620252	24.604086995284234	21.333786922553553
47	12.167609037374188	20.800506768646034	44.614175445182184	22.417708748797597
48	19.617342749219905	23.58961124275626	24.912840485184056	31.880205522839784
49	17.59941815451751	14.59306008493067	43.13656945780448	24.67095230274734
50	23.779180254792013	15.343593834314806	29.15444712948408	31.7227787814091
51	18.502920958168126	15.698331886540132	27.230837810571757	38.567909344719986
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	179.5
2	292.0
3	1284.0
4	2276.0
5	1588.5
6	901.0
7	968.5
8	1036.0
9	1264.5
10	1493.0
11	1609.0
12	1725.0
13	1581.0
14	1437.0
15	1318.5
16	1200.0
17	1196.0
18	1192.0
19	1261.0
20	1330.0
21	1366.5
22	1403.0
23	1555.5
24	1708.0
25	1902.0
26	2392.5
27	2689.0
28	2956.0
29	3223.0
30	3650.0
31	4077.0
32	4580.0
33	5083.0
34	5461.5
35	5840.0
36	6504.0
37	7168.0
38	7978.0
39	8788.0
40	10041.5
41	11295.0
42	12419.5
43	13544.0
44	17137.5
45	20731.0
46	74091.5
47	127452.0
48	96409.0
49	65366.0
50	57957.5
51	50549.0
52	37820.0
53	25091.0
54	20477.0
55	15863.0
56	13863.5
57	11864.0
58	10472.0
59	9080.0
60	8242.0
61	7404.0
62	6248.5
63	5093.0
64	4161.5
65	3230.0
66	2600.5
67	1971.0
68	1473.0
69	975.0
70	807.0
71	639.0
72	592.0
73	545.0
74	385.5
75	188.5
76	151.0
77	112.5
78	74.0
79	63.0
80	52.0
81	31.0
82	10.0
83	5.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	426230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.045633291433553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96044760901135	22.221884869306386
2	10.619963745892658	6.381670725548197
3	4.060136837173628	3.6596814756827905
4	2.2609231586735716	2.7172347250246305
5	1.5945852817574515	2.3955162313800815
6	1.2450689063100768	2.244533026893529
7	0.9938445381212734	2.0902481978760155
8	0.8380878633598519	2.0144704486808944
9	0.7327190084271012	1.9813505969577145
>10	3.4961077794890523	15.780978641631952
>50	0.10534700959941655	2.2307697904249326
>100	0.07154162592199183	4.435084030603402
>500	0.005503201993999371	0.9901934520818846
>1k	0.010220232274570262	6.834035533309418
>5k	0.0031446868537139266	7.220711745393876
>10k+	0.002358515140285445	16.801636509204304
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	28903	6.781080637214649	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG	23652	5.549116674096146	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC	18575	4.357975740797222	RNA PCR Primer, Index 40 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGACTCT	9915	2.3262088543743986	No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	9401	2.205616685826901	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTA	5775	1.3549022828050583	No Hit
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	5478	1.2852215939750837	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC	4680	1.097998733078385	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT	4271	1.002041151490979	No Hit
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3343	0.7843183257865473	RNA PCR Primer, Index 40 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2699	0.6332261924313164	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACAGACTCTCGT	2176	0.5105224878586678	No Hit
TCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	2131	0.49996480773291413	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATG	1824	0.4279379677638834	No Hit
TGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCTT	1658	0.3889918588555475	Illumina PCR Primer Index 8 (96% over 25bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTAT	1395	0.3272880838983647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.31978040025338433	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTT	1222	0.2866996691926894	RNA PCR Primer, Index 40 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCACAGACTC	1113	0.2611266217769749	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	1057	0.24798817539825913	RNA PCR Primer, Index 40 (95% over 24bp)
TCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	702	0.16469980996175773	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCACAGACTCTCG	649	0.15226520892475892	No Hit
TGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	614	0.1440536799380616	TruSeq Adapter, Index 19 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCACAGCCTCTCGTATGCCGTCTTCTGC	567	0.13302676958449663	Illumina PCR Primer Index 3 (95% over 23bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	562	0.13185369401496844	No Hit
TTCCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT	553	0.1297421579898177	Illumina PCR Primer Index 8 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCC	545	0.1278652370785726	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC	473	0.11097294887736668	No Hit
TTCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTG	444	0.10416911057410318	TruSeq Adapter, Index 19 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8941181990943856	0.0
2	0.0	0.0	0.0	4.788259859700162	0.0
3	0.0	0.0	0.0	5.88766628346198	0.0
4	0.0	0.0	0.0	8.693428430659504	0.0
5	0.0	0.0	0.0	15.406001454613707	0.0
6	0.0	0.0	0.0	16.711399948384674	0.0
7	0.0	0.0	0.0	18.76123219857823	0.0
8	0.0	0.0	0.0	21.15031790347934	0.0
9	0.0	0.0	0.0	21.748351828824813	0.0
10	0.0	0.0	0.0	28.863055158013278	0.0
11	0.0	0.0	0.0	30.997114234098962	0.0
12	0.0	0.0	0.0	40.23438049879173	0.0
13	0.0	0.0	0.0	41.287333130000235	0.0
14	0.0	0.0	0.0	41.847828637120806	0.0
15	0.0	0.0	0.0	43.700114961405816	0.0
16	0.0	0.0	0.0	44.8814020599207	0.0
17	0.0	0.0	0.0	45.970954648898484	0.0
18	0.0	0.0	0.0	46.89627665814232	0.0
19	0.0	0.0	0.0	50.25338432301809	0.0
20	0.0	0.0	0.0	51.2441639490416	0.0
21	0.0	0.0	0.0	52.10402834150576	0.0
22	0.0	0.0	0.0	53.060554160899045	0.0
23	0.0	0.0	0.0	53.85097247964714	0.0
24	0.0	0.0	0.0	54.490533280153905	0.0
25	0.0	0.0	0.0	55.03671726532623	0.0
26	0.0	0.0	0.0	55.61363583042019	0.0
27	0.0	0.0	0.0	56.167562114351405	0.0
28	0.0	0.0	0.0	56.63585388170706	0.0
29	0.0	0.0	0.0	57.15740327991929	0.0
30	0.0	0.0	0.0	57.70593341623067	0.0
31	0.0	0.0	0.0	58.25540201299768	0.0
32	0.0	0.0	0.0	58.75395913004716	0.0
33	0.0	0.0	0.0	59.22811627525045	0.0
34	0.0	0.0	0.0	59.67834267883537	0.0
35	0.0	0.0	0.0	60.143584449710254	0.0
36	0.0	0.0	0.0	60.61797621002745	0.0
37	0.0	0.0	0.0	61.08767566806654	0.0
38	0.0	0.0	0.0	61.55854820167515	0.0
39	0.0	0.0	0.0	62.093001431152196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCC	30	2.1622764E-6	45.000004	34
ACTGACG	30	2.1622764E-6	45.000004	43
TTTCGCG	70	0.0	45.000004	1
GGCAATT	30	2.1622764E-6	45.000004	8
CGACCAG	35	1.2095188E-7	45.000004	34
CGTTCCC	30	2.1622764E-6	45.000004	34
TGTTGCG	30	2.1622764E-6	45.000004	1
AAACCGA	30	2.1622764E-6	45.000004	30
CGGGAAC	30	2.1622764E-6	45.000004	6
TATACGG	30	2.1622764E-6	45.000004	27
GTTATTC	30	2.1622764E-6	45.000004	42
CCACCGG	120	0.0	45.000004	15
TGCGTTC	30	2.1622764E-6	45.000004	32
CGGGTAC	25	3.8866623E-5	45.0	6
CTCCGGG	45	3.8380676E-10	45.0	3
TTTGTCG	110	0.0	45.0	1
TTGCTAG	20	7.0282863E-4	45.0	2
CGCGCAA	20	7.0282863E-4	45.0	41
CGACACT	20	7.0282863E-4	45.0	12
CGACACC	20	7.0282863E-4	45.0	34
>>END_MODULE