FastQCFastQC Report
Sat 14 Jan 2017
SRR2933809.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933809.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559018
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC321745.755449735071143No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG294465.267451137530455No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC227024.061049912525179Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT111411.9929590818184746No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC104051.8612996361476735Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA69021.2346650733965632No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT67241.202823522677266Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC57141.0221495551127155No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT51650.9239416262088163No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT42090.7529274549298949Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC39570.7078484055969575Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC28170.503919372900336Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGT23320.41716009144607147No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATG19690.3522247941926736No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.3481104365154611No Hit
TGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCTT17640.31555334533056184Illumina PCR Primer Index 11 (96% over 26bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTAT15040.26904321506642004No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT14220.25437463552157535No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG13580.24292598807194046Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTACAGC12680.22682632759589136No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC7860.14060370149082854No Hit
TCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG7350.13148056055440074Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC6820.12199964938517185No Hit
TTCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT6710.12003191310476587Illumina PCR Primer Index 11 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG6620.11842194705716094Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTTACAGCTCG6390.11430758937994842No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC6150.11001434658633531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGTA207.030173E-445.0000045
GCGAACA207.030173E-445.00000433
GTCCTCG351.2104283E-745.0000041
GTCTAGT207.030173E-445.00000418
GTCACCG207.030173E-445.00000436
ATTGCGT207.030173E-445.00000410
TTCGAAT207.030173E-445.00000412
TGCCCCG253.8882266E-545.01
TTCGTCG502.1827873E-1145.01
GCGGGTC253.8882266E-545.05
CGATCAC253.8882266E-545.034
CGGGTAT650.044.9999966
CTAGAAC302.1634933E-644.9999964
CGTTTTT9750.044.307691
ACCGGTG1850.043.78378317
TACGGCT36100.043.691147
TGATACC33300.043.581084
GATACCT33600.043.3928575
GCGACTG8350.043.38323611
GGCGACT8300.043.37349310
Produced by FastQC (version 0.11.5)