##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933809.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559018
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.24112103724746	28.0	16.0	31.0	16.0	34.0
2	26.074580782729715	30.0	16.0	33.0	16.0	34.0
3	27.13088666196795	31.0	23.0	33.0	16.0	34.0
4	31.333588542766066	35.0	28.0	35.0	19.0	37.0
5	33.32749929340379	35.0	32.0	35.0	28.0	37.0
6	31.707297439438445	35.0	30.0	37.0	17.0	37.0
7	33.215697884504614	35.0	32.0	35.0	28.0	37.0
8	32.16475676990723	35.0	32.0	37.0	17.0	37.0
9	34.24877374252707	35.0	32.0	39.0	28.0	39.0
10	33.57935880418877	35.0	32.0	37.0	27.0	39.0
11	34.246113720846196	35.0	32.0	39.0	27.0	39.0
12	34.28098200773499	37.0	32.0	39.0	27.0	39.0
13	34.3865349595183	35.0	33.0	38.0	27.0	39.0
14	34.23208912772039	36.0	32.0	39.0	25.0	40.0
15	34.81338346886862	37.0	32.0	39.0	27.0	40.0
16	34.53844777806797	36.0	32.0	39.0	27.0	40.0
17	34.02907240911742	36.0	32.0	38.0	27.0	40.0
18	34.37696460579087	36.0	32.0	39.0	27.0	40.0
19	34.14674482753686	36.0	32.0	39.0	27.0	40.0
20	33.979007831590394	36.0	32.0	39.0	25.0	40.0
21	33.840541807240555	36.0	32.0	39.0	25.0	40.0
22	35.0036277901606	37.0	33.0	39.0	27.0	40.0
23	35.475460539732175	37.0	34.0	39.0	29.0	40.0
24	35.76143165336358	37.0	34.0	39.0	30.0	40.0
25	33.79472575122804	36.0	32.0	39.0	24.0	40.0
26	34.70105434887606	37.0	33.0	39.0	27.0	40.0
27	34.970362313914755	37.0	33.0	39.0	27.0	40.0
28	35.151302820302746	37.0	34.0	39.0	27.0	40.0
29	35.53574303510799	37.0	34.0	40.0	29.0	40.0
30	33.85239115735093	36.0	32.0	39.0	24.0	40.0
31	34.66213073639847	37.0	33.0	39.0	25.0	40.0
32	34.85594918231613	37.0	33.0	39.0	27.0	40.0
33	35.314857482227765	37.0	34.0	39.0	29.0	40.0
34	35.39734856480471	38.0	34.0	40.0	29.0	40.0
35	35.567332357813164	38.0	34.0	40.0	29.0	41.0
36	35.50434154177504	38.0	34.0	40.0	29.0	41.0
37	35.51766848294688	38.0	35.0	40.0	30.0	41.0
38	35.372630219420486	38.0	34.0	40.0	30.0	41.0
39	35.282046374177575	37.0	34.0	40.0	29.0	40.0
40	34.87432605032396	37.0	33.0	40.0	27.0	40.0
41	34.10235806360439	37.0	33.0	39.0	24.0	40.0
42	35.22400530931026	37.0	34.0	40.0	29.0	40.0
43	35.250045615704686	38.0	34.0	40.0	28.0	40.0
44	35.81242106694239	38.0	35.0	40.0	30.0	41.0
45	36.01383318605125	38.0	35.0	40.0	31.0	41.0
46	35.7344146342336	38.0	35.0	40.0	29.0	41.0
47	35.001180641768244	37.0	34.0	40.0	26.0	41.0
48	35.34102300820367	38.0	34.0	40.0	28.0	41.0
49	35.71291443209342	38.0	35.0	40.0	29.0	41.0
50	35.23729826230998	38.0	34.0	40.0	27.0	41.0
51	34.77033834330916	37.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	7.0
12	13.0
13	12.0
14	19.0
15	35.0
16	108.0
17	298.0
18	783.0
19	1485.0
20	2199.0
21	2736.0
22	3083.0
23	3390.0
24	3997.0
25	5199.0
26	7078.0
27	9464.0
28	12388.0
29	16571.0
30	22790.0
31	31221.0
32	42302.0
33	53026.0
34	62621.0
35	73410.0
36	79879.0
37	75499.0
38	44575.0
39	4823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.5205699995349	3.0600088011477267	38.70036385232676	13.719057346990615
2	15.748508992554802	30.864122443284476	36.50759009548887	16.879778468671848
3	14.366621468360592	30.77235437857099	39.20142106336469	15.65960308970373
4	15.096293858158413	2.80187757818174	63.25019945690479	18.85162910675506
5	35.62425539070298	3.4546293679273297	38.885509947801324	22.035605293568363
6	15.755843282327223	25.816521113810285	42.20454439749704	16.223091206365446
7	59.09756036478253	1.2323395668833563	34.32125620284141	5.348843865492704
8	55.052252342500594	21.291622094458496	13.964129956459361	9.691995606581541
9	51.695294248127965	3.7869979141995427	14.36268599579978	30.155021841872713
10	39.21537410244393	22.829139669921183	21.092701845021093	16.8627843826138
11	31.886987538862794	18.48151580092233	30.20081643167125	19.430680228543626
12	23.15274284548977	15.572486038016663	31.973389050084254	29.30138206640931
13	24.23249340808346	19.620477337044605	41.763234815336894	14.383794439535041
14	19.378803544787466	29.095127527199484	27.482835973081365	24.043232954931685
15	11.186938524340897	22.190698689487636	45.40301027873879	21.219352507432674
16	14.81973746820317	24.629797251609073	24.791867167067966	35.758598113119795
17	16.107173650937895	27.581222787101666	34.2516698925616	22.059933669398838
18	15.240833032210055	23.081725454278754	33.64757485447696	28.029866659034237
19	15.728652744634342	26.782500742373234	29.00443277318441	28.48441373980802
20	17.24756626799137	27.216833804993755	40.897967507307456	14.637632419707417
21	19.799720223677948	33.6624223191382	29.369358410641517	17.16849904654233
22	14.581820263390446	20.700406784754694	36.92099359949054	27.796779352364325
23	16.60805197685942	31.03138002711898	30.800975997195078	21.559591998826512
24	21.44904099689098	23.521961725740496	25.194179793852793	29.834817483515735
25	15.251208368961287	36.93637771950098	22.369583805888183	25.44283010564955
26	18.6024421396091	20.500592109735287	31.775363226228855	29.121602524426766
27	20.612574192602025	26.2453087378224	28.84826606656673	24.293851003008847
28	13.443574267733776	24.878984218755033	37.47321195381902	24.204229559692177
29	24.691870387000062	21.693755836126925	27.977811090161676	25.63656268671134
30	16.78228608023355	28.423950570464633	35.16380510108798	19.629958248213832
31	21.881585208347495	24.309056237902894	27.74239827697856	26.066960276771052
32	26.825075400076564	33.27012725887181	21.22275132464429	18.68204601640734
33	17.284595487086282	20.31186831193271	23.472589433613944	38.93094676736706
34	28.80336590235019	26.384481358382022	25.028532176065887	19.7836205632019
35	22.04115073217678	21.86423335205664	27.974054502717266	28.120561413049312
36	24.38901788493394	21.20307396184023	28.199628634498353	26.208279518727483
37	15.736702574872366	27.038843114175215	38.742938510030086	18.48151580092233
38	20.307396184022696	21.871209871596264	28.05419503486471	29.767198909516328
39	20.254803959800938	33.778697644798555	24.687040488857246	21.279457906543257
40	24.27274255927358	20.217774740706023	27.065496996518895	28.443985703501497
41	19.235337681434228	28.987080916893554	22.171200211799977	29.606381189872238
42	25.27288924506903	18.426419185070962	33.04186985034471	23.258821719515293
43	24.56378864365727	17.68082601991349	23.95611590324462	33.79926943318462
44	21.279100136310458	18.607093152635514	33.63236961958292	26.481437091471115
45	18.890626062130377	16.05279257555213	30.746952692042118	34.30962867027538
46	28.315009534576703	24.624967353466257	25.927429885978626	21.132593225978415
47	13.17953983592657	18.646805648476438	44.594449552608324	23.57920496298867
48	20.40023755943458	26.569985224089386	25.749081424927283	27.280695791548755
49	21.57819605093217	16.012006769012803	38.91305825572701	23.496738924328017
50	23.458457509418302	16.31181822410012	29.04486080949093	31.18486345699065
51	20.109370360167294	17.144707326061056	26.498252292412765	36.247670021358886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	179.0
2	314.0
3	1730.0
4	3146.0
5	2124.0
6	1102.0
7	1192.0
8	1282.0
9	1534.0
10	1786.0
11	1813.5
12	1841.0
13	1773.5
14	1706.0
15	1622.0
16	1538.0
17	1477.0
18	1416.0
19	1592.0
20	1768.0
21	1860.0
22	1952.0
23	2019.0
24	2086.0
25	2551.5
26	3459.0
27	3901.0
28	4403.5
29	4906.0
30	5400.0
31	5894.0
32	6618.0
33	7342.0
34	8594.0
35	9846.0
36	10800.5
37	11755.0
38	12593.0
39	13431.0
40	15088.0
41	16745.0
42	18966.5
43	21188.0
44	25255.5
45	29323.0
46	92700.0
47	156077.0
48	117750.5
49	79424.0
50	72207.0
51	64990.0
52	48659.0
53	32328.0
54	27510.0
55	22692.0
56	19807.0
57	16922.0
58	15074.5
59	13227.0
60	11096.5
61	8966.0
62	7538.5
63	6111.0
64	5007.5
65	3904.0
66	3301.0
67	2698.0
68	2232.0
69	1766.0
70	1445.5
71	1125.0
72	863.5
73	602.0
74	532.0
75	322.5
76	183.0
77	153.0
78	123.0
79	90.0
80	57.0
81	41.5
82	26.0
83	14.5
84	3.0
85	2.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559018.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.675625333882227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46246754316242	21.065821953702088
2	10.840672640248409	6.217261339980623
3	4.011610483858324	3.4510631766178586
4	2.086209744915844	2.39293476052403
5	1.3136667468458298	1.8835107723065465
6	0.9975139155763597	1.7162601185040913
7	0.8050787887745116	1.6160296397807516
8	0.6904607499830813	1.583951501941293
9	0.638476492397565	1.6477841412435488
>10	4.956170595315648	22.94460370461794
>50	0.10294116525333616	2.03156072681086
>100	0.07767952960834569	4.526189806131558
>500	0.004420786237873332	0.8674608558944572
>1k	0.0069469498023723795	4.445238866134728
>5k	0.0025261635644990466	4.4378138358441905
>10k+	0.0031577044556238085	19.172514799965427
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	32174	5.755449735071143	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCG	29446	5.267451137530455	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTC	22702	4.061049912525179	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTACAGCT	11141	1.9929590818184746	No Hit
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	10405	1.8612996361476735	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTA	6902	1.2346650733965632	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	6724	1.202823522677266	Illumina PCR Primer Index 11 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTC	5714	1.0221495551127155	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCT	5165	0.9239416262088163	No Hit
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	4209	0.7529274549298949	Illumina PCR Primer Index 11 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	3957	0.7078484055969575	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	2817	0.503919372900336	Illumina PCR Primer Index 11 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTACAGCTCGT	2332	0.41716009144607147	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATG	1969	0.3522247941926736	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1946	0.3481104365154611	No Hit
TGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCTT	1764	0.31555334533056184	Illumina PCR Primer Index 11 (96% over 26bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTAT	1504	0.26904321506642004	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTT	1422	0.25437463552157535	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	1358	0.24292598807194046	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTTACAGC	1268	0.22682632759589136	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	786	0.14060370149082854	No Hit
TCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	735	0.13148056055440074	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGC	682	0.12199964938517185	No Hit
TTCCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCT	671	0.12003191310476587	Illumina PCR Primer Index 11 (95% over 22bp)
TGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	662	0.11842194705716094	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTTACAGCTCG	639	0.11430758937994842	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCC	615	0.11001434658633531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8849446708334973	0.0
2	0.0	0.0	0.0	4.187879460053165	0.0
3	0.0	0.0	0.0	5.136507232325256	0.0
4	0.0	0.0	0.0	7.552171844198219	0.0
5	0.0	0.0	0.0	13.57899030084899	0.0
6	0.0	0.0	0.0	14.697380048585197	0.0
7	0.0	0.0	0.0	16.49284996189747	0.0
8	0.0	0.0	0.0	18.679541624777734	0.0
9	0.0	0.0	0.0	19.202243934900128	0.0
10	0.0	0.0	0.0	25.824392058931913	0.0
11	0.0	0.0	0.0	27.84454167844327	0.0
12	0.0	0.0	0.0	35.76718459870702	0.0
13	0.0	0.0	0.0	36.68969514398463	0.0
14	0.0	0.0	0.0	37.16284627686407	0.0
15	0.0	0.0	0.0	38.8134192458919	0.0
16	0.0	0.0	0.0	39.81678586378256	0.0
17	0.0	0.0	0.0	40.76666583186946	0.0
18	0.0	0.0	0.0	41.599197163597594	0.0
19	0.0	0.0	0.0	44.546329456296576	0.0
20	0.0	0.0	0.0	45.48493966205024	0.0
21	0.0	0.0	0.0	46.36326558357692	0.0
22	0.0	0.0	0.0	47.38147966612882	0.0
23	0.0	0.0	0.0	48.25443903416348	0.0
24	0.0	0.0	0.0	48.90647528344347	0.0
25	0.0	0.0	0.0	49.44581390939111	0.0
26	0.0	0.0	0.0	49.94257787763542	0.0
27	0.0	0.0	0.0	50.56545585294212	0.0
28	0.0	0.0	0.0	51.09459802725494	0.0
29	0.0	0.0	0.0	51.68241451974713	0.0
30	0.0	0.0	0.0	52.30690246110143	0.0
31	0.0	0.0	0.0	52.8219127112186	0.0
32	0.0	0.0	0.0	53.32100218597612	0.0
33	0.0	0.0	0.0	53.803276459791995	0.0
34	0.0	0.0	0.0	54.31399346711555	0.0
35	0.0	0.0	0.0	54.84188344561356	0.0
36	1.7888511640054525E-4	0.0	0.0	55.30519589709097	0.0
37	1.7888511640054525E-4	0.0	0.0	55.81358739790132	0.0
38	1.7888511640054525E-4	0.0	0.0	56.35847146245738	0.0
39	1.7888511640054525E-4	0.0	0.0	57.25558032120612	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGGTA	20	7.030173E-4	45.000004	5
GCGAACA	20	7.030173E-4	45.000004	33
GTCCTCG	35	1.2104283E-7	45.000004	1
GTCTAGT	20	7.030173E-4	45.000004	18
GTCACCG	20	7.030173E-4	45.000004	36
ATTGCGT	20	7.030173E-4	45.000004	10
TTCGAAT	20	7.030173E-4	45.000004	12
TGCCCCG	25	3.8882266E-5	45.0	1
TTCGTCG	50	2.1827873E-11	45.0	1
GCGGGTC	25	3.8882266E-5	45.0	5
CGATCAC	25	3.8882266E-5	45.0	34
CGGGTAT	65	0.0	44.999996	6
CTAGAAC	30	2.1634933E-6	44.999996	4
CGTTTTT	975	0.0	44.30769	1
ACCGGTG	185	0.0	43.783783	17
TACGGCT	3610	0.0	43.69114	7
TGATACC	3330	0.0	43.58108	4
GATACCT	3360	0.0	43.392857	5
GCGACTG	835	0.0	43.383236	11
GGCGACT	830	0.0	43.373493	10
>>END_MODULE