SRR2933808.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507596
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	31364	6.178929699997636	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	27951	5.506544574819344	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	21878	4.310120647128819	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	10807	2.1290553905074114	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	10347	2.0384321389451454	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTA	6824	1.3443762362193556	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	6471	1.274832740998747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC	5537	1.0908281389136243	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	5305	1.0451224989952639	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	4391	0.8650580382824135	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	4313	0.8496914869305511	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	3244	0.6390909305825894	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGT	2314	0.455874356771921	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATG	1890	0.3723433596797453	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1890	0.3723433596797453	No Hit
TGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCTT	1724	0.33964018628988407	TruSeq Adapter, Index 23 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	1701	0.3351090237117708	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTAT	1651	0.3252586702810897	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	1524	0.3002387725671597	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCTCTTG	1266	0.24941094886484527	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	801	0.1578026619595111	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	746	0.1469672731857619	TruSeq Adapter, Index 20 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	744	0.14657325904853466	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCG	712	0.14026903285289877	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC	710	0.1398750187156715	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	658	0.12963065114776318	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	617	0.12155336133460469	TruSeq Adapter, Index 20 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	539	0.10618680998274219	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	35	1.2101373E-7	45.000004	1
GTTTGCG	85	0.0	45.000004	1
ATGGGCG	25	3.887716E-5	45.000004	5
GTTCTCG	25	3.887716E-5	45.000004	1
CGACGAA	25	3.887716E-5	45.000004	19
GAGTCGC	25	3.887716E-5	45.000004	17
GGCACGT	25	3.887716E-5	45.000004	8
CGTTGTA	25	3.887716E-5	45.000004	23
TAGCGGG	65	0.0	45.000004	3
TCGGGTT	35	1.2101373E-7	45.000004	5
CGACCCG	50	2.1827873E-11	45.000004	37
GACCCGT	50	2.1827873E-11	45.000004	38
CCGAGGA	65	0.0	45.000004	18
GCGATTC	35	1.2101373E-7	45.000004	9
CGCGTTG	25	3.887716E-5	45.000004	21
CCACGTA	25	3.887716E-5	45.000004	34
ACGACCC	50	2.1827873E-11	45.000004	36
AGTCGCG	25	3.887716E-5	45.000004	18
CTTCGTG	20	7.0295593E-4	45.0	1
CTTCGAA	40	6.8030204E-9	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content