##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933808.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507596
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.210559184863552	28.0	16.0	31.0	16.0	34.0
2	25.946362855499256	30.0	16.0	33.0	16.0	34.0
3	26.98208811732165	31.0	16.0	33.0	16.0	34.0
4	31.277687373422957	33.0	28.0	35.0	19.0	37.0
5	33.359311342090955	35.0	32.0	35.0	28.0	37.0
6	31.790130339876594	35.0	30.0	37.0	17.0	37.0
7	33.32079646017699	35.0	32.0	35.0	29.0	37.0
8	32.2840053901134	35.0	32.0	37.0	17.0	37.0
9	34.4442529097944	35.0	32.0	39.0	29.0	39.0
10	33.63831669280294	35.0	32.0	37.0	27.0	39.0
11	34.44765522186936	35.0	32.0	39.0	27.0	39.0
12	34.64491839967218	37.0	34.0	39.0	27.0	39.0
13	34.908478396204856	37.0	34.0	39.0	30.0	39.0
14	34.63714844088606	37.0	33.0	39.0	27.0	40.0
15	35.29349325053783	37.0	34.0	39.0	27.0	40.0
16	34.83139150032703	36.0	32.0	39.0	27.0	40.0
17	34.21442052340838	36.0	32.0	38.0	27.0	40.0
18	34.65025729123161	36.0	32.0	39.0	27.0	40.0
19	34.41892568105344	36.0	32.0	39.0	27.0	40.0
20	34.31427552620588	36.0	32.0	39.0	26.0	40.0
21	34.03684426197212	36.0	32.0	39.0	25.0	40.0
22	35.301893631943514	37.0	34.0	39.0	29.0	40.0
23	35.62759162798761	37.0	34.0	39.0	29.0	40.0
24	35.95567143949125	38.0	34.0	39.0	30.0	40.0
25	33.94884908470516	36.0	32.0	39.0	24.0	40.0
26	34.8496560256582	37.0	33.0	39.0	27.0	40.0
27	35.15470571084091	37.0	33.0	39.0	29.0	40.0
28	35.24870960370058	37.0	34.0	39.0	27.0	40.0
29	35.70980267772008	38.0	34.0	40.0	29.0	40.0
30	34.010476835908875	37.0	32.0	39.0	24.0	40.0
31	34.881374163705	37.0	33.0	39.0	27.0	40.0
32	35.34882071568728	37.0	34.0	39.0	30.0	40.0
33	35.73314013506805	38.0	35.0	40.0	30.0	40.0
34	35.944006650958634	38.0	35.0	40.0	30.0	40.0
35	35.98542738713465	38.0	35.0	40.0	30.0	41.0
36	35.90785782393872	38.0	35.0	40.0	30.0	41.0
37	35.91285392319877	38.0	35.0	40.0	30.0	41.0
38	35.86841503873159	38.0	35.0	40.0	30.0	41.0
39	35.59556812898447	38.0	34.0	40.0	30.0	41.0
40	35.46734804844798	38.0	34.0	40.0	29.0	41.0
41	34.97260813717996	37.0	33.0	40.0	27.0	40.0
42	35.820672345723764	38.0	35.0	40.0	30.0	41.0
43	35.79956106825113	38.0	35.0	40.0	30.0	41.0
44	36.33491201664316	39.0	35.0	40.0	31.0	41.0
45	36.59789872260617	39.0	36.0	40.0	32.0	41.0
46	36.30824907997699	39.0	35.0	40.0	31.0	41.0
47	35.77042175273249	38.0	35.0	40.0	28.0	41.0
48	36.037663811377556	38.0	35.0	40.0	30.0	41.0
49	36.3398371933585	39.0	35.0	40.0	31.0	41.0
50	35.96755293579934	38.0	35.0	40.0	29.0	41.0
51	35.45220805522502	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	2.0
11	10.0
12	8.0
13	9.0
14	16.0
15	30.0
16	68.0
17	233.0
18	556.0
19	1159.0
20	1730.0
21	2086.0
22	2341.0
23	2437.0
24	2985.0
25	3911.0
26	5404.0
27	7329.0
28	9861.0
29	13489.0
30	18327.0
31	25224.0
32	34437.0
33	45175.0
34	56336.0
35	68818.0
36	77817.0
37	76663.0
38	46345.0
39	4787.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.764931165730225	2.76755529988416	39.671904427930876	12.795609106454739
2	13.593487734339908	32.32767791708366	37.74044712724292	16.3383872213335
3	12.764481989613788	32.23547860897249	40.26844183169292	14.731597569720803
4	14.760163594669775	2.746672550611116	65.1900330183847	17.303130836334407
5	36.17778705899968	3.1875743701684014	39.74913119882741	20.885507372004508
6	14.85334793812402	26.585906902339655	42.23949755317221	16.321247606364118
7	57.5045902646987	0.8953971268489113	35.90808438206763	5.691928226384762
8	53.89423872528546	21.748201325463555	14.003459444124855	10.354100505126123
9	51.300049645781286	2.43087021962348	14.796806909431911	31.472273225163317
10	39.38289505827469	14.53892465661668	25.12628153098133	20.9518987541273
11	29.590067691628775	22.204075682235477	28.683638168937502	19.522218457198246
12	19.75251972040757	18.260388182727997	32.555024074263784	29.43206802260065
13	21.265534007360184	21.872709792827365	42.68296046462147	14.17879573519098
14	18.19695190663441	30.60800321515536	25.99488569649879	25.20015918171144
15	9.613944948344747	24.909573755506347	44.567727090047995	20.908754206100916
16	11.238465236132672	26.611320814190815	25.261231372981662	36.88898257669485
17	12.316290908517798	29.635182310341296	36.36159465401619	21.686932127124724
18	11.223886713055265	25.084910046572475	35.404731321759826	28.28647191861244
19	11.888588562557624	28.83040843505465	30.347559870448155	28.933443131939573
20	14.355511075737398	27.3357158054831	43.251916878777614	15.056856240001892
21	18.275754734079857	35.26899345148504	28.846563014680964	17.608688799754134
22	12.929573913112002	22.577601084326908	35.98905428726783	28.503770715293264
23	14.867138432926975	33.053451957856254	30.056383423037218	22.02302618617956
24	20.017691234761504	23.529145225730698	26.20056107613141	30.252602463376387
25	12.355495315171908	38.00010244367568	23.608933088519215	26.035469152633194
26	17.33386393903813	21.858131269749958	32.69154997281302	28.116454818398886
27	19.559649800234833	27.317394148102036	28.282334770171552	24.84062128149158
28	12.395684757169088	24.460003624930064	36.68626230309143	26.45804931480942
29	23.792937690604337	16.959353501603637	32.24257086344258	27.005137944349443
30	13.965831094019654	27.69052553605623	36.05721873300814	22.28642463691597
31	20.09531201979527	19.102790408119844	28.60050118598255	32.20139638610233
32	20.58645064184903	27.987218181388346	32.11353123350066	19.312799943261965
33	16.7071844537782	16.991268646719043	27.275628649555944	39.025918249946805
34	26.56600918840968	18.6841503873159	33.235880503392465	21.513959920881963
35	16.329915917383115	17.811212066288938	38.43272996635119	27.426142049976754
36	28.33060150198189	18.002111915775536	26.38633874183406	27.280947840408516
37	13.433320987557035	17.030473053373154	50.192081891898276	19.34412406717153
38	17.621100245076793	19.388647664678206	25.926721250758476	37.06353083948652
39	20.43790731211436	19.84353698610706	41.239686679958076	18.478869021820504
40	16.18491871488349	17.803725797681622	37.32614126194848	28.685214225486412
41	23.08981946272232	21.39358860195904	32.307583196085076	23.209008739233564
42	18.644748973593174	15.918762165186488	43.15518640808832	22.281302453132017
43	32.02133192538948	16.294060630895437	24.952521296464116	26.73208614725096
44	20.04192310420098	16.002884183484504	37.1876452927131	26.767547419601417
45	17.317512352343204	14.16796034641723	31.868651447213924	36.64587585402565
46	29.14089157518972	20.74346527553409	31.3678594787981	18.747783670478096
47	12.92189063743607	15.841338387221334	47.35143696955847	23.885334005784127
48	19.81832008132452	21.2137211483148	31.313091513723514	27.654867256637168
49	17.274367804316817	13.037533786712267	45.73322090796618	23.954877501004738
50	25.987990449097314	13.252074484432502	32.31585749296685	28.444077573503336
51	19.239119299600468	14.019811030819787	30.297126060883066	36.443943608696685
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	183.5
2	276.0
3	1584.0
4	2892.0
5	1934.5
6	977.0
7	1083.5
8	1190.0
9	1360.0
10	1530.0
11	1524.0
12	1518.0
13	1561.0
14	1604.0
15	1469.5
16	1335.0
17	1377.0
18	1419.0
19	1376.0
20	1333.0
21	1529.0
22	1725.0
23	2045.0
24	2365.0
25	2661.5
26	3562.5
27	4167.0
28	5037.0
29	5907.0
30	6816.0
31	7725.0
32	8518.5
33	9312.0
34	9983.0
35	10654.0
36	11502.5
37	12351.0
38	13593.0
39	14835.0
40	16563.0
41	18291.0
42	20404.0
43	22517.0
44	25849.0
45	29181.0
46	91296.0
47	153411.0
48	115175.0
49	76939.0
50	67663.0
51	58387.0
52	41305.5
53	24224.0
54	19471.0
55	14718.0
56	11856.0
57	8994.0
58	7459.0
59	5924.0
60	4800.0
61	3676.0
62	3017.5
63	2359.0
64	1761.0
65	1163.0
66	967.5
67	772.0
68	571.5
69	371.0
70	322.0
71	273.0
72	185.0
73	97.0
74	84.5
75	60.0
76	48.0
77	27.5
78	7.0
79	6.0
80	5.0
81	3.0
82	1.0
83	1.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	507596.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.455429719927327
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.96749245712687	21.49288845910228
2	11.740750493528482	6.916577020426606
3	4.41392402995036	3.900420871599037
4	2.264524712851113	2.6681019411369826
5	1.4727575815414073	2.1690353718791533
6	1.0263955196265844	1.8139752655925485
7	0.8161954195330933	1.6828970772448542
8	0.7151081355000456	1.6851053941895888
9	0.6018343335510392	1.5954560023456743
>10	3.7755162915348763	17.745746747467518
>50	0.11086239520397702	2.2880399174080024
>100	0.0757109040417404	4.5975843436559725
>500	0.005407921717267171	1.1005131514675859
>1k	0.00743589236124236	5.158692731721044
>5k	0.0027039608586335853	4.8060586099100995
>10k+	0.003379951073291982	20.378907094853087
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	31364	6.178929699997636	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	27951	5.506544574819344	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	21878	4.310120647128819	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	10807	2.1290553905074114	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	10347	2.0384321389451454	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTA	6824	1.3443762362193556	No Hit
GAACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	6471	1.274832740998747	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTC	5537	1.0908281389136243	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	5305	1.0451224989952639	No Hit
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	4391	0.8650580382824135	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	4313	0.8496914869305511	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	3244	0.6390909305825894	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGT	2314	0.455874356771921	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATG	1890	0.3723433596797453	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1890	0.3723433596797453	No Hit
TGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCTT	1724	0.33964018628988407	TruSeq Adapter, Index 23 (96% over 26bp)
GAATGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTT	1701	0.3351090237117708	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTAT	1651	0.3252586702810897	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	1524	0.3002387725671597	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGTCTCTTG	1266	0.24941094886484527	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	801	0.1578026619595111	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	746	0.1469672731857619	TruSeq Adapter, Index 20 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCT	744	0.14657325904853466	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTCTCTTGTCG	712	0.14026903285289877	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCC	710	0.1398750187156715	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	658	0.12963065114776318	No Hit
TGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	617	0.12155336133460469	TruSeq Adapter, Index 20 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTG	539	0.10618680998274219	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9531201979527025	0.0
2	0.0	0.0	0.0	4.717728272090403	0.0
3	0.0	0.0	0.0	5.778020315368916	0.0
4	0.0	0.0	0.0	8.459483526268922	0.0
5	0.0	0.0	0.0	14.865759383446678	0.0
6	0.0	0.0	0.0	16.199891252098126	0.0
7	0.0	0.0	0.0	18.27949786838352	0.0
8	0.0	0.0	0.0	20.717263335408475	0.0
9	0.0	0.0	0.0	21.265928021497412	0.0
10	0.0	0.0	0.0	28.29297315187669	0.0
11	0.0	0.0	0.0	30.63243209166345	0.0
12	0.0	0.0	0.0	39.078913151403874	0.0
13	0.0	0.0	0.0	40.0310483140135	0.0
14	0.0	0.0	0.0	40.53459838138993	0.0
15	0.0	0.0	0.0	42.28776428498254	0.0
16	0.0	0.0	0.0	43.45936532202776	0.0
17	0.0	0.0	0.0	44.63451248630801	0.0
18	0.0	0.0	0.0	45.63195927469878	0.0
19	0.0	0.0	0.0	48.85992009393297	0.0
20	0.0	0.0	0.0	49.896965303115074	0.0
21	0.0	0.0	0.0	50.97892812394109	0.0
22	0.0	0.0	0.0	52.18067124248418	0.0
23	0.0	0.0	0.0	53.23485606663567	0.0
24	0.0	0.0	0.0	54.02032324919818	0.0
25	0.0	0.0	0.0	54.73526190119701	0.0
26	0.0	0.0	0.0	55.38952237606285	0.0
27	0.0	0.0	0.0	55.99571312618697	0.0
28	0.0	0.0	0.0	56.55403115863797	0.0
29	0.0	0.0	0.0	57.14564338568468	0.0
30	0.0	0.0	0.0	57.81999858154911	0.0
31	0.0	0.0	0.0	58.45180025059299	0.0
32	0.0	0.0	0.0	58.985295392398676	0.0
33	0.0	0.0	0.0	59.53258102900732	0.0
34	0.0	0.0	0.0	60.07494148890062	0.0
35	0.0	0.0	0.0	60.608830644843536	0.0
36	0.0	0.0	0.0	61.159662408687225	0.0
37	0.0	0.0	0.0	61.689611423257865	0.0
38	0.0	0.0	0.0	62.202420822859125	0.0
39	0.0	0.0	0.0	62.721534448656016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTTCG	35	1.2101373E-7	45.000004	1
GTTTGCG	85	0.0	45.000004	1
ATGGGCG	25	3.887716E-5	45.000004	5
GTTCTCG	25	3.887716E-5	45.000004	1
CGACGAA	25	3.887716E-5	45.000004	19
GAGTCGC	25	3.887716E-5	45.000004	17
GGCACGT	25	3.887716E-5	45.000004	8
CGTTGTA	25	3.887716E-5	45.000004	23
TAGCGGG	65	0.0	45.000004	3
TCGGGTT	35	1.2101373E-7	45.000004	5
CGACCCG	50	2.1827873E-11	45.000004	37
GACCCGT	50	2.1827873E-11	45.000004	38
CCGAGGA	65	0.0	45.000004	18
GCGATTC	35	1.2101373E-7	45.000004	9
CGCGTTG	25	3.887716E-5	45.000004	21
CCACGTA	25	3.887716E-5	45.000004	34
ACGACCC	50	2.1827873E-11	45.000004	36
AGTCGCG	25	3.887716E-5	45.000004	18
CTTCGTG	20	7.0295593E-4	45.0	1
CTTCGAA	40	6.8030204E-9	45.0	13
>>END_MODULE