##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933805.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1302087
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.03494697358932	30.0	16.0	33.0	16.0	34.0
2	27.06869279856108	31.0	25.0	33.0	16.0	34.0
3	28.10156157000262	31.0	25.0	34.0	16.0	34.0
4	31.889614134846596	35.0	28.0	35.0	19.0	37.0
5	33.48310289558225	35.0	33.0	37.0	28.0	37.0
6	32.29424301141168	35.0	32.0	37.0	17.0	37.0
7	33.18213836709836	35.0	32.0	35.0	28.0	37.0
8	32.740914393585065	35.0	32.0	37.0	25.0	37.0
9	34.36696165463598	35.0	32.0	39.0	28.0	39.0
10	33.228185981428275	35.0	31.0	37.0	27.0	39.0
11	33.6833637076478	35.0	32.0	38.0	27.0	39.0
12	33.97749459137523	35.0	32.0	39.0	26.0	39.0
13	34.13391731888883	35.0	32.0	38.0	27.0	39.0
14	34.317847424941654	37.0	32.0	39.0	25.0	40.0
15	34.77143616363576	37.0	32.0	39.0	27.0	40.0
16	34.69237539427089	36.0	32.0	39.0	27.0	40.0
17	34.14859606155349	36.0	32.0	38.0	26.0	40.0
18	34.39295377344217	36.0	32.0	39.0	27.0	40.0
19	34.2231156597063	36.0	32.0	39.0	27.0	40.0
20	34.205385661633976	36.0	32.0	39.0	26.0	40.0
21	33.939413418611814	36.0	32.0	39.0	25.0	40.0
22	34.851328674658454	37.0	33.0	39.0	27.0	40.0
23	35.51369685742965	37.0	34.0	39.0	29.0	40.0
24	35.7798726198787	38.0	34.0	39.0	30.0	40.0
25	33.80089732867312	36.0	32.0	39.0	24.0	40.0
26	34.499508865383035	36.0	33.0	39.0	27.0	40.0
27	34.911633400840344	37.0	33.0	39.0	27.0	40.0
28	35.17603048029817	37.0	34.0	39.0	27.0	40.0
29	35.64543152646482	38.0	34.0	40.0	29.0	40.0
30	33.57980687926383	36.0	32.0	39.0	23.0	40.0
31	34.2609679691142	36.0	32.0	39.0	25.0	40.0
32	34.508145769061514	37.0	33.0	39.0	26.0	40.0
33	35.151112790466385	37.0	34.0	39.0	27.0	40.0
34	35.02637842171836	38.0	34.0	40.0	26.0	40.0
35	35.22192526305846	38.0	34.0	40.0	27.0	41.0
36	35.21911208698036	38.0	34.0	40.0	27.0	41.0
37	35.4028386736063	38.0	34.0	40.0	29.0	41.0
38	35.06687264368664	37.0	34.0	40.0	28.0	41.0
39	34.794344771125125	37.0	34.0	40.0	26.0	41.0
40	34.4187861486982	37.0	33.0	40.0	25.0	41.0
41	33.674041749898436	36.0	32.0	39.0	23.0	40.0
42	34.65682247038792	37.0	33.0	40.0	26.0	41.0
43	34.903401232022134	37.0	34.0	40.0	27.0	40.0
44	35.49094338550343	38.0	35.0	40.0	29.0	41.0
45	35.80832540375566	38.0	35.0	40.0	30.0	41.0
46	35.47104072154933	38.0	35.0	40.0	29.0	41.0
47	34.97601696353623	37.0	34.0	40.0	26.0	41.0
48	35.113807295518654	37.0	34.0	40.0	27.0	41.0
49	35.440114216638364	38.0	34.0	40.0	28.0	41.0
50	35.097190126312604	37.0	34.0	40.0	27.0	41.0
51	34.551294959553395	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	12.0
10	28.0
11	26.0
12	17.0
13	35.0
14	70.0
15	145.0
16	332.0
17	898.0
18	2143.0
19	3934.0
20	5660.0
21	6812.0
22	7674.0
23	8661.0
24	10784.0
25	14109.0
26	18572.0
27	23730.0
28	30148.0
29	39740.0
30	53436.0
31	72190.0
32	95903.0
33	118722.0
34	137328.0
35	164024.0
36	181709.0
37	180424.0
38	111671.0
39	13144.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23378391766449	3.1344295734463214	41.61311801745966	16.018668491429526
2	17.992960531823144	24.20183904762124	40.53776744564687	17.267432974908743
3	16.541598218859416	24.182408702337092	42.37312867726965	16.902864401533847
4	15.671840668096678	3.4377887191869667	60.567304642470134	20.32306597024623
5	31.774912121847464	4.3388805817122815	41.85933812410384	22.02686917233641
6	18.70136173696535	21.204881087054858	44.53788418131814	15.555872994661646
7	66.03675484049838	1.737979105850838	27.93177414412401	4.293491909526783
8	64.17274728954364	17.089180676867212	12.16815773446782	6.569914299121334
9	60.03308534683166	3.922625753885877	12.293725380869327	23.75056351841313
10	43.06670752415161	21.29074324526702	19.895982372913636	15.746566857667728
11	35.38020116935351	18.838756550061557	27.084211730859764	18.69683054972517
12	26.72171675164563	16.023276478453436	29.865439098923495	27.38956767097744
13	27.252403257232427	17.58453928193738	38.27017703118148	16.89288042964871
14	21.27415449198095	25.619639855094167	29.232301681838464	23.87390397108642
15	15.131554189543403	20.862814850313384	42.40722778124657	21.598403178896646
16	19.482876336220237	22.994623247140936	26.631246606409558	30.89125381022927
17	20.554847717548828	25.518417740135646	33.52717598747242	20.399558554843107
18	19.186966769501577	22.528141360753928	32.10353839643588	26.181353473308622
19	19.18573797296187	26.1537823509489	28.713365543162627	25.9471141329266
20	21.237444195357146	26.350696996437257	37.14329380448464	15.26856500372095
21	22.545958910579706	30.573533104930778	29.512467292892104	17.36804069159741
22	18.47126958490485	20.230829429984325	36.143437420080225	25.1544635650306
23	20.427744075472685	28.215395745445583	31.304052647787746	20.052807531293993
24	22.53850933155772	23.243224147080802	26.964096869103216	27.25416965225826
25	18.740299227317376	33.288405459850225	24.277102835678416	23.694192477153983
26	18.042649991897623	21.962510953569154	32.455819004413684	27.53902005011954
27	21.730882805834018	27.06793017670862	27.218380953039233	23.98280606441812
28	16.500279935211704	25.27465522657088	34.18926692302435	24.035797915193072
29	21.998606851923107	21.642255855407512	30.567696321367162	25.791440971302226
30	22.15297441722404	29.97334279506669	28.87295549375733	19.00072729395194
31	26.437634351621664	22.5010310370966	25.15822675443346	25.903107856848273
32	26.504987761954464	28.659528894766634	23.140082037528984	21.69540130574992
33	25.872003944436894	21.810524181563903	22.43429202503366	29.883179848965547
34	24.184559096281586	26.089961730667767	29.145825125356446	20.579654047694202
35	19.157475652548563	30.447735059178072	24.377249753664692	26.01753953460867
36	23.997782022245826	25.171052318316672	27.405234826858727	23.425930832578775
37	18.45314483594414	30.054136167552553	27.7089011717343	23.783817824769006
38	30.64464970466643	23.038245524300606	21.422608473934538	24.894496297098428
39	21.871810408981887	26.311068308031643	24.929516998480132	26.887604284506335
40	25.059001433851964	26.223286155226187	26.908570625465117	21.80914178545673
41	19.747681990527514	29.71445072410676	22.898239518557514	27.63962776680821
42	18.996426506062956	26.121910440700198	31.092238844255416	23.789424208981426
43	21.923573463217128	20.523743805137446	26.252393273260545	31.300289458384885
44	22.044149123676068	22.36432742205398	30.033861024647358	25.557662429622596
45	21.398339742275287	19.493013907672836	27.559602392159665	31.549043957892213
46	26.35131139470711	24.42670881438798	25.428408393601963	23.793571397302944
47	17.33056239713629	21.97833170901791	39.020587718024984	21.670518175820817
48	21.279914475760837	27.149184347896878	25.900266264850195	25.67063491149209
49	20.426515278932975	20.754757554602726	35.31699494734223	23.501732219122072
50	23.11266451473673	19.809659415999086	28.440803110698443	28.63687295856575
51	20.173075992618006	20.283898080543004	27.281894374185445	32.26113155265355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	722.5
2	1250.0
3	6999.5
4	12749.0
5	8699.0
6	4649.0
7	4427.5
8	4206.0
9	4211.5
10	4217.0
11	4102.5
12	3988.0
13	3823.5
14	3659.0
15	3522.5
16	3386.0
17	3405.5
18	3425.0
19	3471.0
20	3517.0
21	3827.5
22	4138.0
23	4445.0
24	4752.0
25	5658.5
26	7695.0
27	8825.0
28	10526.0
29	12227.0
30	14209.0
31	16191.0
32	18324.5
33	20458.0
34	22833.5
35	25209.0
36	26932.5
37	28656.0
38	31556.0
39	34456.0
40	37960.5
41	41465.0
42	44972.0
43	48479.0
44	55907.5
45	63336.0
46	169103.5
47	274871.0
48	211285.0
49	147699.0
50	136253.5
51	124808.0
52	104276.5
53	83745.0
54	75635.5
55	67526.0
56	62804.5
57	58083.0
58	54378.5
59	50674.0
60	46062.5
61	41451.0
62	36498.5
63	31546.0
64	26732.5
65	21919.0
66	18065.0
67	14211.0
68	12049.0
69	9887.0
70	8316.0
71	6745.0
72	5352.0
73	3959.0
74	3157.0
75	1864.0
76	1373.0
77	1040.5
78	708.0
79	501.0
80	294.0
81	209.0
82	124.0
83	87.5
84	51.0
85	39.5
86	28.0
87	27.5
88	27.0
89	15.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1302087.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.82624596376096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32620441490366	22.168716544316926
2	9.570210267448505	5.708868907236595
3	3.375602954190567	3.02044691963058
4	1.7634959906554144	2.1039386069357873
5	1.1820717527449816	1.7628381422092925
6	0.9243440985313887	1.6541828662768856
7	0.7947888033241696	1.6593896436032947
8	0.7621646690490615	1.8186008707156625
9	0.6489543859290643	1.7420285820583552
>10	6.5108151118942805	32.78372781729729
>50	0.08356003295203114	1.7115130786866286
>100	0.045553223958205366	2.6967113615721656
>500	0.005206082731331338	1.0122566565174456
>1k	0.004425170321631637	2.8744269208655764
>5k	7.809124096997007E-4	1.8408971914929553
>10k+	0.0018221289559659684	15.441455890584576
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGC	57089	4.384422853465245	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCG	48767	3.74529505324913	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	37899	2.91063500365183	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCGAACT	17804	1.3673433495611276	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	14193	1.0900193305055654	RNA PCR Primer, Index 33 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCT	11711	0.8994022672832154	RNA PCR Primer, Index 33 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTA	11425	0.8774375291359179	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTC	9037	0.694039645584358	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCT	7767	0.5965039202449606	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6907	0.5304561062356048	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGT	4018	0.3085815310344086	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	3714	0.28523439677993867	Illumina Paired End PCR Primer 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	3651	0.2803960104048347	TruSeq Adapter, Index 1 (95% over 24bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATG	3537	0.27164083505940845	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTT	2708	0.2079738143457388	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTAT	2519	0.1934586552204269	No Hit
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2370	0.18201548744438734	RNA PCR Primer, Index 33 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	2125	0.16319954043009416	TruSeq Adapter, Index 1 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCGCACTCGTATGC	2010	0.15436756530093612	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTAGCGAAC	1915	0.14707158584641425	No Hit
TGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCTT	1518	0.11658207170488608	RNA PCR Primer, Index 33 (96% over 26bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1354	0.1039869071728694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.385611714117413	0.0
2	0.0	0.0	0.0	2.0525510200163275	0.0
3	0.0	0.0	0.0	2.5270968836951755	0.0
4	0.0	0.0	0.0	4.036980631862541	0.0
5	0.0	0.0	0.0	8.170191392741039	0.0
6	0.0	0.0	0.0	8.955622780966248	0.0
7	0.0	0.0	0.0	10.206076859687563	0.0
8	0.0	0.0	0.0	11.733778157680709	0.0
9	0.0	0.0	0.0	12.192964064613195	0.0
10	0.0	0.0	0.0	16.95485785512028	0.0
11	0.0	0.0	0.0	18.308377243609684	0.0
12	0.0	0.0	0.0	24.366190584807313	0.0
13	0.0	0.0	0.0	25.050246258506537	0.0
14	0.0	0.0	0.0	25.426181199873742	0.0
15	0.0	0.0	0.0	26.650600151909973	0.0
16	0.0	0.0	0.0	27.387340477249214	0.0
17	7.6799783731809E-5	0.0	0.0	28.045437824047088	0.0
18	7.6799783731809E-5	0.0	0.0	28.58979469113815	0.0
19	7.6799783731809E-5	0.0	0.0	30.6479520953669	0.0
20	1.53599567463618E-4	0.0	0.0	31.33246856776851	0.0
21	1.53599567463618E-4	0.0	0.0	32.01990343195194	0.0
22	1.53599567463618E-4	0.0	0.0	32.82238437216561	0.0
23	1.53599567463618E-4	0.0	0.0	33.46719535637788	0.0
24	1.53599567463618E-4	0.0	0.0	34.00771223428235	0.0
25	2.3039935119542704E-4	0.0	0.0	34.47834130899087	0.0
26	2.3039935119542704E-4	0.0	0.0	34.961488748447685	0.0
27	2.3039935119542704E-4	0.0	0.0	35.45700095308531	0.0
28	2.3039935119542704E-4	0.0	0.0	35.89460612078916	0.0
29	2.3039935119542704E-4	0.0	0.0	36.40394228649852	0.0
30	2.3039935119542704E-4	0.0	0.0	36.993841425342545	0.0
31	2.3039935119542704E-4	0.0	0.0	37.49211842219452	0.0
32	2.3039935119542704E-4	0.0	0.0	37.95022913215477	0.0
33	2.3039935119542704E-4	0.0	0.0	38.40780224362888	0.0
34	2.3039935119542704E-4	0.0	0.0	38.909228031613864	0.0
35	2.3039935119542704E-4	0.0	0.0	39.43569054909541	0.0
36	2.3039935119542704E-4	0.0	0.0	39.91584279698668	0.0
37	2.3039935119542704E-4	0.0	0.0	40.4219533717793	0.0
38	2.3039935119542704E-4	0.0	0.0	40.99626215452577	0.0
39	2.3039935119542704E-4	0.0	0.0	41.97599699559246	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCG	20	7.033632E-4	45.0	1
CGTTTTT	4060	0.0	43.66995	1
TACGGCT	6800	0.0	43.44485	7
TGATACC	5600	0.0	43.151787	4
GATACCT	5615	0.0	42.996437	5
ACGGCTG	6880	0.0	42.972385	8
CTCGAAT	75	0.0	42.0	43
CCGATGA	1745	0.0	41.647564	18
CGGCTGT	7315	0.0	41.555023	9
ACCGGTG	400	0.0	41.062504	17
CGATGAA	1740	0.0	40.99138	19
ATACCTG	5915	0.0	40.85376	6
ATGATAC	21915	0.0	40.657085	3
AATGATC	1625	0.0	40.56923	2
GAATCTG	4540	0.0	40.539646	1
AATGATA	21805	0.0	40.335934	2
GAATGAT	23020	0.0	40.17159	1
ATACGGC	16295	0.0	39.863457	6
ACCACCT	2520	0.0	39.73214	14
TGATACG	16455	0.0	39.694622	4
>>END_MODULE