SRR2933804.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933804.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359906
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	31580	8.774513345151234	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	23579	6.551432874139358	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	22297	6.195228754174701	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	11251	3.1260940356648685	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT	10049	2.7921179419070534	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	7709	2.141948175356899	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACACTATCGTA	6725	1.8685434530127312	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	5466	1.518729890582541	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	4669	1.2972831794968687	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	4152	1.1536345601351463	Illumina PCR Primer Index 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3073	0.8538340566703528	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3059	0.8499441520841554	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACACTATCGT	2399	0.6665629358777014	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATG	2222	0.6173834278950615	No Hit
TGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCTT	2043	0.5676482192572505	TruSeq Adapter, Index 13 (95% over 24bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCACACTATCGTAT	1502	0.41733119203347546	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCACACTA	1236	0.34342300489572275	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1080	0.3000783537923791	No Hit
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	978	0.2717376203786544	No Hit
TCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	803	0.22311381305118558	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	705	0.19588448094780306	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.19143887570643445	No Hit
TGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	687	0.19088317505126337	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCACACTATCG	631	0.17532355670647337	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	619	0.1719893527754469	No Hit
GCACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	561	0.1558740337754858	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	522	0.1450378709996499	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	463	0.1286447016721033	No Hit
GCTGTCTCTTATACACATCTGACGCGCACCCTATCGTATGCCGTCTTCTGC	441	0.12253199446522148	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	437	0.12142059315487932	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	430	0.11947564086178057	No Hit
TTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	418	0.11614143693075415	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	411	0.11419648463765537	No Hit
GACTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	401	0.11141798136180003	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	401	0.11141798136180003	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	399	0.11086228070662896	No Hit
GCCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	391	0.10863947808594467	No Hit
GCATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	385	0.10697237612043145	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT	378	0.1050274238273327	No Hit
ACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	377	0.10474957349974716	No Hit
GGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	375	0.10419387284457608	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	25	3.8854476E-5	45.000004	1
TTGATAA	20	7.026822E-4	45.000004	21
TTCTAGG	25	3.8854476E-5	45.000004	2
CGGGTAC	20	7.026822E-4	45.000004	6
CCGGGAC	20	7.026822E-4	45.000004	5
GGAGCAC	20	7.026822E-4	45.000004	8
ATGATTA	25	3.8854476E-5	45.000004	25
ATTAGGC	20	7.026822E-4	45.000004	28
GAAGATC	20	7.026822E-4	45.000004	9
CGACGAA	20	7.026822E-4	45.000004	19
ACTGACG	20	7.026822E-4	45.000004	43
TTTCGCG	40	6.7921064E-9	45.000004	1
GACCGAT	25	3.8854476E-5	45.000004	9
GGTTTCG	20	7.026822E-4	45.000004	1
GTAGGCT	20	7.026822E-4	45.000004	7
TTTCCCG	20	7.026822E-4	45.000004	1
TACGGAG	20	7.026822E-4	45.000004	29
TCTAGAC	20	7.026822E-4	45.000004	28
AATGCTA	165	0.0	45.000004	2
CACTCTA	20	7.026822E-4	45.000004	27

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content