##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933804.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359906
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	23.534964685223365	25.0	16.0	31.0	16.0	33.0
2	24.250251454546465	26.0	16.0	31.0	16.0	34.0
3	25.36954371419204	28.0	16.0	31.0	16.0	34.0
4	30.23895683873011	32.0	28.0	35.0	19.0	37.0
5	32.968916883853005	35.0	32.0	35.0	28.0	37.0
6	30.804901835479264	35.0	28.0	35.0	17.0	37.0
7	33.016809944818924	35.0	32.0	35.0	28.0	37.0
8	31.485443421337795	35.0	28.0	35.0	17.0	37.0
9	34.033166993603885	35.0	32.0	37.0	28.0	39.0
10	33.715959167115855	35.0	32.0	37.0	28.0	39.0
11	34.46472412240974	35.0	32.0	39.0	27.0	39.0
12	34.56244963962813	37.0	34.0	39.0	27.0	39.0
13	34.7154479225131	35.0	34.0	38.0	30.0	39.0
14	34.41678660539141	36.0	32.0	38.0	27.0	40.0
15	35.080779425738946	37.0	33.0	39.0	27.0	40.0
16	34.3784210321584	36.0	32.0	38.0	27.0	40.0
17	33.93414947236223	36.0	32.0	38.0	27.0	40.0
18	34.290325807294124	36.0	32.0	38.0	27.0	40.0
19	33.88716776047079	35.0	32.0	39.0	27.0	40.0
20	33.708412752218635	35.0	31.0	38.0	25.0	40.0
21	33.84563191500003	36.0	32.0	38.0	25.0	40.0
22	35.26507199101988	37.0	34.0	39.0	29.0	40.0
23	35.37110523303307	37.0	33.0	39.0	29.0	40.0
24	35.631256494751405	37.0	34.0	39.0	30.0	40.0
25	33.65725494990359	36.0	32.0	39.0	24.0	40.0
26	34.79515762449084	37.0	33.0	39.0	27.0	40.0
27	34.74429712202631	37.0	33.0	39.0	27.0	40.0
28	34.522814290398046	37.0	33.0	39.0	25.0	40.0
29	35.13563541591416	37.0	33.0	39.0	27.0	40.0
30	33.1120737081349	36.0	31.0	39.0	21.0	40.0
31	33.99213127872278	36.0	31.0	39.0	25.0	40.0
32	34.340813990319695	36.0	32.0	39.0	25.0	40.0
33	34.60831439320267	37.0	33.0	39.0	25.0	40.0
34	34.82155062710819	37.0	34.0	39.0	26.0	40.0
35	35.1416258689769	38.0	34.0	40.0	27.0	40.0
36	35.29283479575223	38.0	34.0	40.0	27.0	40.0
37	35.723816774379976	38.0	35.0	40.0	30.0	40.0
38	34.72315271209705	37.0	33.0	39.0	27.0	40.0
39	34.920301412035364	37.0	33.0	40.0	27.0	40.0
40	34.30162320161376	37.0	33.0	39.0	25.0	40.0
41	33.61176807277456	36.0	32.0	39.0	24.0	40.0
42	34.7628603024123	37.0	33.0	39.0	27.0	40.0
43	34.8678905047429	37.0	34.0	39.0	27.0	40.0
44	35.5213583546815	38.0	34.0	40.0	28.0	40.0
45	35.70113029513262	38.0	35.0	40.0	29.0	40.0
46	35.267019721816254	38.0	34.0	40.0	28.0	40.0
47	34.41898717998588	37.0	33.0	40.0	25.0	41.0
48	34.92094602479536	37.0	34.0	40.0	28.0	41.0
49	35.2722960995371	37.0	34.0	40.0	28.0	41.0
50	34.90146038132179	37.0	34.0	40.0	26.0	41.0
51	34.38373630892511	36.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	7.0
11	9.0
12	14.0
13	11.0
14	11.0
15	22.0
16	44.0
17	146.0
18	402.0
19	784.0
20	1268.0
21	1604.0
22	1865.0
23	2123.0
24	2683.0
25	3812.0
26	5351.0
27	7101.0
28	9395.0
29	12374.0
30	16351.0
31	22026.0
32	29876.0
33	38279.0
34	46356.0
35	52862.0
36	50425.0
37	37297.0
38	16069.0
39	1337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	56.32804121076058	3.4206153829055364	29.48631031435986	10.765033091974015
2	13.419059421071058	42.211577467449835	27.059843403555373	17.309519707923734
3	11.942284929953932	42.24186315315666	32.16784382588787	13.64800809100154
4	14.687168316171444	2.672086600390102	65.40207720904903	17.238667874389424
5	40.66895244869494	3.104977410768367	34.079731930003945	22.14633821053275
6	14.044500508466099	33.267575422471424	36.07636438403361	16.61155968502887
7	46.31237045228476	1.2333776041521953	45.87642328830306	6.577828655259984
8	37.84488171911555	28.43325757281068	18.875762004523402	14.846098703550373
9	35.53650119753492	3.7104132745772502	19.333937194711954	41.41914833317589
10	38.83875234088901	14.662995337671504	26.945646918917713	19.55260540252177
11	32.17284513178441	18.14223713969759	28.750284796585774	20.934632931932224
12	18.665707156868738	13.448511555795124	34.58180747195101	33.30397381538513
13	20.31891660600268	21.994909781998633	45.714436547320695	11.971737064677999
14	18.19558440259401	32.83273965980006	23.032402905203025	25.939273032402905
15	7.421382249809673	23.73425283268409	47.764416264246776	21.07994865325946
16	8.009869243635839	25.521663990041844	23.19522319716815	43.27324356915417
17	9.549160058459709	30.761365467649888	35.569287536190004	24.1201869377004
18	8.595577734186149	24.830094524681446	34.24949848015871	32.3248292609737
19	9.67724905947664	28.67193100420665	28.469933816051967	33.18088612026474
20	12.10927297683284	26.604724566970265	46.84806588386968	14.437936572327217
21	17.685451201146964	37.15164515178964	26.7942184903836	18.368685156679803
22	10.117086128044544	22.294710285463427	36.250020838774574	31.338182747717457
23	14.398759676137656	35.191411090673675	27.63138152739882	22.778447705789844
24	22.904869604841263	22.67675448589354	21.507560307413602	32.910815601851596
25	10.614438214422654	41.863708857312744	17.600151150578206	29.921701777686394
26	18.235039149111156	20.547865275933162	28.461320455896814	32.75577511905887
27	21.379193456069085	28.890599212016472	18.408695603852117	31.32151172806233
28	11.906720088022983	29.822509210738357	32.103382549888025	26.16738815135063
29	26.409673637005216	16.384000266736315	19.603174162142338	37.60315193411613
30	13.31681050051958	36.23557262174012	24.255500047234555	26.19211683050574
31	21.040493906742316	27.808927886725975	14.877773640895123	36.272804565636584
32	20.44089289981273	34.967463726639735	17.778253210560536	26.813390162986998
33	11.056498085611242	31.561296560768643	15.046428789739544	42.335776563880565
34	28.49966380110362	22.19857407211883	21.191922335276434	28.109839791501113
35	12.659138775124616	21.975460259067646	25.591126571938226	39.77427439386951
36	24.571138019371723	36.42951215039483	17.153923524475836	21.845426305757616
37	11.289614510455507	18.683489577834212	31.79496868626808	38.2319272254422
38	16.39872633409835	40.171322511989246	17.227553861286	26.202397292626404
39	20.231949453468406	23.803993264908062	22.489205514773303	33.474851766850236
40	15.508771734841876	28.59441076281029	31.809139052974945	24.08767844937289
41	15.236756264135634	32.32177290737025	18.362572449472918	34.07889837902119
42	16.78132623518363	16.713808605580347	41.687551749623516	24.81731340961251
43	26.523870121642872	28.347123971259165	18.410918406472803	26.718087500625167
44	18.586241963179276	16.583774652270314	30.639111323512253	34.19087206103816
45	16.464854712063705	16.340377765305387	26.89896806388335	40.29579945874756
46	31.590470845165125	22.396125655032147	24.558634754630376	21.454768745172352
47	9.918423143820887	18.886042466644067	49.313709690863725	21.88182469867132
48	18.971898217868	23.2519046639956	23.52003023011564	34.256166888020765
49	16.61767239223575	11.71027990642001	47.88805966002234	23.7839880413219
50	25.169905475318554	15.305107444721678	30.39015742999561	29.134829649964157
51	18.09528043433563	12.659138775124616	26.575828132901368	42.669752657638384
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	92.5
2	138.0
3	610.0
4	1082.0
5	802.0
6	522.0
7	768.0
8	1014.0
9	1216.5
10	1419.0
11	1550.0
12	1681.0
13	1678.0
14	1675.0
15	1497.0
16	1319.0
17	1309.5
18	1300.0
19	1264.5
20	1229.0
21	1284.0
22	1339.0
23	1322.5
24	1306.0
25	1456.0
26	1627.5
27	1649.0
28	1669.5
29	1690.0
30	1712.5
31	1735.0
32	2143.5
33	2552.0
34	2873.0
35	3194.0
36	3250.0
37	3306.0
38	3841.5
39	4377.0
40	5410.0
41	6443.0
42	7652.5
43	8862.0
44	11726.5
45	14591.0
46	73678.0
47	132765.0
48	97784.0
49	62803.0
50	55247.5
51	47692.0
52	33488.0
53	19284.0
54	15082.5
55	10881.0
56	8988.5
57	7096.0
58	6186.5
59	5277.0
60	4507.0
61	3737.0
62	3087.0
63	2437.0
64	2038.0
65	1639.0
66	1266.0
67	893.0
68	690.5
69	488.0
70	414.5
71	341.0
72	258.5
73	176.0
74	162.5
75	104.0
76	59.0
77	62.5
78	66.0
79	51.5
80	37.0
81	19.0
82	1.0
83	4.5
84	8.0
85	4.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.76787272232194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23789483528559	17.88244708340511
2	10.408921933085502	4.947958633643229
3	4.104416543919945	2.926597500458453
4	2.1229337635313645	2.018304779581335
5	1.3934675364148605	1.655987952409796
6	0.9258609805709476	1.3203447566864681
7	0.6955647518178205	1.1572466143937583
8	0.5868462275841108	1.1158469155835136
9	0.4757896705711814	1.0177657499458193
>10	3.7420214631409134	15.339005184687112
>50	0.14145098314278368	2.3411668602357283
>100	0.1332678684155152	6.520313637449779
>500	0.01052114750648804	1.7212827793923968
>1k	0.011690163896097823	7.067123082138115
>5k	0.0035070491688293467	5.529221518952171
>10k+	0.005845081948048911	27.439386951037214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	31580	8.774513345151234	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	23579	6.551432874139358	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	22297	6.195228754174701	No Hit
GCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	11251	3.1260940356648685	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCACACTAT	10049	2.7921179419070534	No Hit
GAACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	7709	2.141948175356899	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCACACTATCGTA	6725	1.8685434530127312	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTC	5466	1.518729890582541	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCT	4669	1.2972831794968687	No Hit
CTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCT	4152	1.1536345601351463	Illumina PCR Primer Index 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3073	0.8538340566703528	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	3059	0.8499441520841554	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCACACTATCGT	2399	0.6665629358777014	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATG	2222	0.6173834278950615	No Hit
TGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGCTT	2043	0.5676482192572505	TruSeq Adapter, Index 13 (95% over 24bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCACACTATCGTAT	1502	0.41733119203347546	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCACACTA	1236	0.34342300489572275	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTT	1080	0.3000783537923791	No Hit
CGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	978	0.2717376203786544	No Hit
TCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	803	0.22311381305118558	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	705	0.19588448094780306	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	689	0.19143887570643445	No Hit
TGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	687	0.19088317505126337	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGCACACTATCG	631	0.17532355670647337	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	619	0.1719893527754469	No Hit
GCACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	561	0.1558740337754858	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGC	522	0.1450378709996499	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCG	463	0.1286447016721033	No Hit
GCTGTCTCTTATACACATCTGACGCGCACCCTATCGTATGCCGTCTTCTGC	441	0.12253199446522148	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCC	437	0.12142059315487932	No Hit
GGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	430	0.11947564086178057	No Hit
TTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	418	0.11614143693075415	No Hit
TGCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	411	0.11419648463765537	No Hit
GACTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	401	0.11141798136180003	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	401	0.11141798136180003	No Hit
GTTCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	399	0.11086228070662896	No Hit
GCCCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCT	391	0.10863947808594467	No Hit
GCATCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTC	385	0.10697237612043145	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGT	378	0.1050274238273327	No Hit
ACTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTGC	377	0.10474957349974716	No Hit
GGCTGTCTCTTATACACATCTGACGCGCACACTATCGTATGCCGTCTTCTG	375	0.10419387284457608	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.29533822720377	0.0
2	0.0	0.0	0.0	6.806777325190466	0.0
3	0.0	0.0	0.0	8.249098375686986	0.0
4	0.0	0.0	0.0	12.636910748917773	0.0
5	0.0	0.0	0.0	22.18857146032575	0.0
6	0.0	0.0	0.0	23.82566559045973	0.0
7	0.0	0.0	0.0	26.335209749212293	0.0
8	0.0	0.0	0.0	29.594671942118218	0.0
9	0.0	0.0	0.0	30.255955721771794	0.0
10	0.0	0.0	0.0	38.65814962795841	0.0
11	0.0	0.0	0.0	41.27772251643485	0.0
12	0.0	0.0	0.0	52.966885797958355	0.0
13	0.0	0.0	0.0	54.26194617483454	0.0
14	0.0	0.0	0.0	54.89461137074681	0.0
15	0.0	0.0	0.0	57.2902368951893	0.0
16	0.0	0.0	0.0	58.64392369118603	0.0
17	0.0	0.0	0.0	59.753380049235076	0.0
18	0.0	0.0	0.0	60.60165709935372	0.0
19	0.0	0.0	0.0	64.55296660794762	0.0
20	0.0	0.0	0.0	65.5104388368074	0.0
21	0.0	0.0	0.0	66.27258228537451	0.0
22	0.0	0.0	0.0	67.09668635699322	0.0
23	0.0	0.0	0.0	67.73518640978477	0.0
24	0.0	0.0	0.0	68.24615316221458	0.0
25	0.0	0.0	0.0	68.64570193328258	0.0
26	0.0	0.0	0.0	69.05136341155746	0.0
27	0.0	0.0	0.0	69.42590565314276	0.0
28	0.0	0.0	0.0	69.77349641295227	0.0
29	0.0	0.0	0.0	70.1322011858652	0.0
30	0.0	0.0	0.0	70.55203303084694	0.0
31	0.0	0.0	0.0	70.94296844175979	0.0
32	0.0	0.0	0.0	71.29305985451757	0.0
33	0.0	0.0	0.0	71.6150883841892	0.0
34	0.0	0.0	0.0	71.96045634137802	0.0
35	0.0	0.0	0.0	72.29665523775653	0.0
36	0.0	0.0	0.0	72.60173489744544	0.0
37	0.0	0.0	0.0	72.9312653859619	0.0
38	0.0	0.0	0.0	73.23134373975427	0.0
39	0.0	0.0	0.0	73.56226347990864	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTCG	25	3.8854476E-5	45.000004	1
TTGATAA	20	7.026822E-4	45.000004	21
TTCTAGG	25	3.8854476E-5	45.000004	2
CGGGTAC	20	7.026822E-4	45.000004	6
CCGGGAC	20	7.026822E-4	45.000004	5
GGAGCAC	20	7.026822E-4	45.000004	8
ATGATTA	25	3.8854476E-5	45.000004	25
ATTAGGC	20	7.026822E-4	45.000004	28
GAAGATC	20	7.026822E-4	45.000004	9
CGACGAA	20	7.026822E-4	45.000004	19
ACTGACG	20	7.026822E-4	45.000004	43
TTTCGCG	40	6.7921064E-9	45.000004	1
GACCGAT	25	3.8854476E-5	45.000004	9
GGTTTCG	20	7.026822E-4	45.000004	1
GTAGGCT	20	7.026822E-4	45.000004	7
TTTCCCG	20	7.026822E-4	45.000004	1
TACGGAG	20	7.026822E-4	45.000004	29
TCTAGAC	20	7.026822E-4	45.000004	28
AATGCTA	165	0.0	45.000004	2
CACTCTA	20	7.026822E-4	45.000004	27
>>END_MODULE