FastQCFastQC Report
Sat 14 Jan 2017
SRR2933802.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933802.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171823
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC325342.7763578629195704No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG265532.2659565480452253No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC215871.842172409997073No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC133911.1427493742655674No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT105460.8999652677921496No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC73610.6281665405099576No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT69620.594117029619661No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA65940.5627129694501645No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT65540.5592994846491322No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC59740.5098039550341646No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC54090.4615884822195843No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT50370.4298430735699845No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT29540.2520858525562308Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGT23470.20028622070056654No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG20230.17263699381220543No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG19690.1680287893308119No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.15215608500601202No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG17550.14976664564528944No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT14820.1264696118782444No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG14480.12356814979736701No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTAT14460.12339747555731541No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCACATCGTATGCCGTCTTCTGC14310.1221174187569283No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC13700.11691185443535414No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTAACA13360.11401039235447676No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC253.8908518E-545.0000045
ATCCGGC650.045.0000046
CATTGCG207.033343E-445.01
TAGCGCG207.033343E-445.01
TACGGGT900.045.04
TCCGTCG207.033343E-445.01
CGATAGC207.033343E-445.010
TCGCGAG406.8157533E-945.01
ATGCGAA302.1655396E-644.99999626
TACGGCT36000.043.6257
ACGGCTG36650.042.8512958
TCCGTTG1700.042.3529431
CCCGCTT750.042.040
CTACGGC1450.041.8965536
ATACGGC70600.041.876776
GATACGG71200.041.650285
TGATACG71550.041.6037754
ACGGGTA650.041.5384645
CGTTTTT18700.041.3903731
ATGATAC102100.041.363863
Produced by FastQC (version 0.11.5)