##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933802.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1171823
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.03152438550873	31.0	16.0	33.0	16.0	34.0
2	28.303384555517344	31.0	25.0	33.0	16.0	34.0
3	29.193566775869733	31.0	28.0	34.0	16.0	34.0
4	32.6577392660837	35.0	32.0	37.0	28.0	37.0
5	33.879955419888496	35.0	33.0	37.0	28.0	37.0
6	33.025708660778974	35.0	33.0	37.0	26.0	37.0
7	33.61104108726318	35.0	32.0	35.0	30.0	37.0
8	33.71740186017854	35.0	35.0	37.0	28.0	37.0
9	34.80190011631449	37.0	33.0	39.0	29.0	39.0
10	33.74001363687178	35.0	32.0	39.0	27.0	39.0
11	34.34516560948198	37.0	32.0	39.0	27.0	39.0
12	34.45981005663825	37.0	33.0	39.0	27.0	39.0
13	34.95563237792739	37.0	34.0	39.0	30.0	39.0
14	34.700153521478924	37.0	33.0	39.0	27.0	40.0
15	35.17780074294497	37.0	33.0	39.0	27.0	40.0
16	34.71515834729306	36.0	32.0	39.0	27.0	40.0
17	34.50640241742994	36.0	32.0	39.0	27.0	40.0
18	34.61435728774738	37.0	32.0	39.0	27.0	40.0
19	34.42860909881441	37.0	32.0	39.0	27.0	40.0
20	35.28054236860004	37.0	33.0	39.0	28.0	40.0
21	34.495213867623356	37.0	32.0	39.0	25.0	40.0
22	35.53574387940841	37.0	34.0	39.0	29.0	40.0
23	36.305114338940264	38.0	34.0	39.0	30.0	40.0
24	36.43402800593605	38.0	35.0	40.0	31.0	40.0
25	34.188565167265025	37.0	32.0	39.0	24.0	40.0
26	34.98671642389678	37.0	33.0	39.0	27.0	40.0
27	35.793478195939144	37.0	34.0	39.0	30.0	40.0
28	35.97091881623761	38.0	35.0	40.0	30.0	40.0
29	36.45376477505562	38.0	35.0	40.0	30.0	41.0
30	34.12211997887053	37.0	32.0	39.0	24.0	40.0
31	35.0323982376178	37.0	33.0	39.0	27.0	40.0
32	35.66342783850462	37.0	34.0	39.0	30.0	40.0
33	36.321613417726056	38.0	35.0	40.0	30.0	40.0
34	36.57830747476368	38.0	35.0	40.0	31.0	41.0
35	36.77410154946609	39.0	35.0	40.0	31.0	41.0
36	36.7070129191866	39.0	35.0	40.0	31.0	41.0
37	37.008456055223355	39.0	36.0	40.0	32.0	41.0
38	36.43239123997395	38.0	35.0	40.0	30.0	41.0
39	36.41101599814989	38.0	35.0	40.0	31.0	41.0
40	35.943455624270904	38.0	35.0	40.0	30.0	41.0
41	35.480575991425326	37.0	34.0	40.0	29.0	41.0
42	36.44221866271613	38.0	35.0	40.0	31.0	41.0
43	36.55129144930591	38.0	35.0	40.0	31.0	41.0
44	36.96106323224583	39.0	36.0	40.0	32.0	41.0
45	37.166676195978404	39.0	36.0	40.0	33.0	41.0
46	36.943119396018	39.0	35.0	40.0	32.0	41.0
47	36.80481096547857	39.0	35.0	40.0	31.0	41.0
48	36.877333863561304	39.0	35.0	40.0	32.0	41.0
49	37.066050930899976	39.0	35.0	40.0	32.0	41.0
50	36.92131149499541	39.0	35.0	40.0	32.0	41.0
51	36.213745591271035	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	8.0
10	17.0
11	24.0
12	19.0
13	33.0
14	32.0
15	69.0
16	122.0
17	230.0
18	499.0
19	906.0
20	1396.0
21	2091.0
22	2763.0
23	3593.0
24	4822.0
25	6811.0
26	9399.0
27	12389.0
28	16906.0
29	24018.0
30	33740.0
31	47686.0
32	66980.0
33	91201.0
34	119328.0
35	161002.0
36	205844.0
37	217789.0
38	128566.0
39	13537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.844655720189824	3.8864231202152544	49.96710254023005	14.30181861936487
2	17.917040372138114	14.950722080041098	46.62709299954003	20.505144548280757
3	17.771625919614138	15.049713139271034	48.411236167919554	18.76742477319527
4	17.094902557809498	3.763793678738171	56.35825547032274	22.783048293129593
5	24.62880486216775	4.658382707968696	47.3594561636015	23.353356266262054
6	18.441607648936742	14.324091607691605	49.986730077835986	17.247570665535665
7	73.51827025071192	1.2134085096469347	20.52639349116718	4.741927748473959
8	73.03210467792492	9.655383108199787	9.838943253375296	7.473568960500007
9	68.67538868924744	3.6809313351931134	11.657989303845376	15.985690671714073
10	34.874038143985906	23.409252079878957	24.00823332534009	17.708476450795043
11	27.44561251997955	24.036223900708553	27.147188611249312	21.370974968062583
12	20.276867752211725	19.861019966325973	34.09149675334927	25.770615528113034
13	21.73749789857342	22.861387769313286	38.25902034692953	17.14209398518377
14	21.369524237022144	25.698420324571202	29.52869161980948	23.403363818597178
15	16.335060841099722	25.135109995280857	38.06607311855118	20.46375604506824
16	17.068533387721523	25.15482287000682	29.186489768505997	28.590153973765663
17	17.814209142507018	26.515267237458218	32.386887780833796	23.283635839200972
18	17.291348608108905	24.255454962054852	33.84154432879368	24.611652101042562
19	18.869061283146003	26.889385171651348	29.948464913216416	24.29308863198623
20	19.84463523928102	25.92533172671982	36.173722481978935	18.05631055202023
21	21.962958569681597	28.94737515819369	29.47100372667203	19.618662545452683
22	18.739860883426935	23.723975378534128	31.29645006114405	26.239713676894887
23	18.36267081291287	28.597748977447957	29.159437901457814	23.880142308181355
24	21.750639815057394	23.455931484533075	27.82007180265279	26.973356897756744
25	17.399385402061572	30.252094386268237	25.634844170151975	26.713676041518212
26	19.89728824233694	23.883385118742336	30.277354173795874	25.941972465124852
27	19.830298603116685	25.799459474681758	28.098270813936914	26.271971108264648
28	16.034930189968964	23.57830491465008	35.90832403869867	24.47844085668228
29	21.688343717438556	21.154986717277268	30.688764429440283	26.467905135843893
30	18.225619398151427	27.111261683718446	30.53430424219357	24.128814675936553
31	19.869895026808656	26.764878313533696	28.72165847572543	24.643568183932217
32	19.300781773356558	29.661561515689655	27.656736554923395	23.380920156030392
33	16.361003325587568	28.73505640356948	25.881297772786503	29.022642498056445
34	21.843315927405417	26.450069677758503	28.609525500011518	23.097088894824562
35	17.088502273807563	24.32543140047601	26.494615654411973	32.09145067130446
36	21.463480406170557	27.51447957584038	27.633695532516427	23.388344485472633
37	15.58921441207418	24.67821505466269	34.59507109862155	25.13749943464158
38	16.84640086429435	28.813737228233272	30.304235366603997	24.035626540868375
39	18.70751811493715	29.08792539487619	32.17303295804913	20.031523532137534
40	18.62354638883176	27.802492355927473	27.81392753001093	25.760033725229835
41	17.764116253051867	31.81444637970069	24.591256529356396	25.83018083789105
42	18.617316779069874	23.218694290861333	29.767976904361838	28.396012025706952
43	21.477731705214868	25.82301251980888	27.111944380678654	25.5873113942976
44	19.186771381002078	22.286471591699428	31.013472171138474	27.51328485616002
45	19.328431000244915	20.38925673928571	29.6765808488142	30.605731411655174
46	23.530772138795705	24.712776588273144	28.938670771951053	22.8177805009801
47	15.425964501464811	23.84404470641044	36.99517759934734	23.734813192777406
48	18.25651143560077	25.745014392105293	28.68769430195516	27.31077987033878
49	18.23022760263282	20.772164396841504	35.79900718794562	25.198600812580057
50	20.906997046482275	21.27992026099505	30.19372379617058	27.619358896352093
51	18.110670297476666	20.99284618922824	27.483587538391035	33.41289597490406
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	148.0
1	372.0
2	596.0
3	2045.5
4	3495.0
5	2531.0
6	1567.0
7	1622.0
8	1677.0
9	1694.0
10	1711.0
11	1810.0
12	1909.0
13	1913.0
14	1917.0
15	2000.5
16	2084.0
17	2090.5
18	2097.0
19	2262.0
20	2427.0
21	2707.5
22	2988.0
23	3612.0
24	4236.0
25	4813.0
26	6451.5
27	7513.0
28	8814.5
29	10116.0
30	12492.0
31	14868.0
32	17666.0
33	20464.0
34	23321.0
35	26178.0
36	29999.0
37	33820.0
38	37826.5
39	41833.0
40	48978.5
41	56124.0
42	67288.5
43	78453.0
44	149728.0
45	221003.0
46	195906.5
47	170810.0
48	159880.0
49	148950.0
50	121103.5
51	93257.0
52	78100.5
53	62944.0
54	53238.0
55	43532.0
56	38284.5
57	33037.0
58	29102.5
59	25168.0
60	22097.5
61	19027.0
62	16070.5
63	13114.0
64	10584.5
65	8055.0
66	6510.5
67	4966.0
68	3719.5
69	2473.0
70	2001.0
71	1529.0
72	1193.5
73	858.0
74	769.5
75	562.0
76	443.0
77	326.0
78	209.0
79	139.5
80	70.0
81	48.0
82	26.0
83	29.0
84	32.0
85	17.5
86	3.0
87	10.0
88	17.0
89	10.5
90	4.0
91	3.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1171823.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.23457210022326
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.10904906696135	22.599351970270554
2	12.965393307832723	8.358678107781719
3	5.12920604458194	4.960132861829325
4	2.609471367884039	3.36460771806105
5	1.4293255267486003	2.303684837333367
6	0.9753249667940592	1.886350977796257
7	0.7206324084209852	1.6260494128902645
8	0.5818044153227792	1.5003373099160289
9	0.42522169294904	1.2336155387950343
>10	4.860274676399545	29.5383977568575
>50	0.11087516971572398	2.4984491962371314
>100	0.07222743637066065	4.4237048706315365
>500	0.004530872388493816	1.016684923870743
>1k	0.0034647847676717424	1.9318216324283866
>5k	0.0018656533364386303	3.770772181309006
>10k+	0.001332609526027593	8.987360703992081
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGC	32534	2.7763578629195704	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCG	26553	2.2659565480452253	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTC	21587	1.842172409997073	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	13391	1.1427493742655674	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTTAACAT	10546	0.8999652677921496	No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	7361	0.6281665405099576	No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCT	6962	0.594117029619661	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTA	6594	0.5627129694501645	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	6554	0.5592994846491322	No Hit
TCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC	5974	0.5098039550341646	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTC	5409	0.4615884822195843	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCT	5037	0.4298430735699845	No Hit
TGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGCTT	2954	0.2520858525562308	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCTTAACATCGT	2347	0.20028622070056654	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	2023	0.17263699381220543	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATG	1969	0.1680287893308119	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	0.15215608500601202	No Hit
TCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1755	0.14976664564528944	No Hit
TTCCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCT	1482	0.1264696118782444	No Hit
TGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG	1448	0.12356814979736701	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCTTAACATCGTAT	1446	0.12339747555731541	No Hit
GCTGTCTCTTATACACATCTGACGCGCTTCACATCGTATGCCGTCTTCTGC	1431	0.1221174187569283	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCC	1370	0.11691185443535414	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCTTAACA	1336	0.11401039235447676	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6845743768470153	0.0
2	0.0	0.0	0.0	3.3930892293460704	0.0
3	0.0	0.0	0.0	4.256188861287072	0.0
4	0.0	0.0	0.0	5.919750679070132	0.0
5	0.0	0.0	0.0	9.403894615483738	0.0
6	0.0	0.0	0.0	10.369740139935809	0.0
7	0.0	0.0	0.0	11.662597508326769	0.0
8	0.0	0.0	0.0	13.421480889178655	0.0
9	0.0	0.0	0.0	13.94715754853762	0.0
10	0.0	0.0	0.0	17.541813055384644	0.0
11	8.533712002580594E-5	0.0	0.0	19.838320292399107	0.0
12	8.533712002580594E-5	0.0	0.0	24.69169831962677	0.0
13	8.533712002580594E-5	0.0	0.0	25.559150144689088	0.0
14	8.533712002580594E-5	0.0	0.0	25.89179423854968	0.0
15	8.533712002580594E-5	0.0	0.0	26.859943865242446	0.0
16	8.533712002580594E-5	0.0	0.0	27.868457949707423	0.0
17	8.533712002580594E-5	0.0	0.0	29.217040457475232	0.0
18	8.533712002580594E-5	0.0	0.0	30.60965691917636	0.0
19	8.533712002580594E-5	0.0	0.0	32.50866385111062	0.0
20	8.533712002580594E-5	0.0	0.0	33.60720859720282	0.0
21	8.533712002580594E-5	0.0	0.0	34.97285852897579	0.0
22	8.533712002580594E-5	0.0	0.0	36.47445049294987	0.0
23	8.533712002580594E-5	0.0	0.0	37.985941562846946	0.0
24	8.533712002580594E-5	0.0	0.0	39.11256222142764	0.0
25	8.533712002580594E-5	0.0	0.0	40.09794994636562	0.0
26	8.533712002580594E-5	0.0	0.0	40.95379592310443	0.0
27	8.533712002580594E-5	0.0	0.0	41.81006858544337	0.0
28	1.706742400516119E-4	0.0	0.0	42.70687637979456	0.0
29	1.706742400516119E-4	0.0	0.0	43.604964230946145	0.0
30	1.706742400516119E-4	0.0	0.0	44.53411479378712	0.0
31	1.706742400516119E-4	0.0	0.0	45.4378348948604	0.0
32	1.706742400516119E-4	0.0	0.0	46.27576007639379	0.0
33	1.706742400516119E-4	0.0	0.0	47.090985584000315	0.0
34	1.706742400516119E-4	0.0	0.0	47.893666534963046	0.0
35	1.706742400516119E-4	0.0	0.0	48.7149509780914	0.0
36	1.706742400516119E-4	0.0	0.0	49.48605719464458	0.0
37	1.706742400516119E-4	0.0	0.0	50.2794363995245	0.0
38	1.706742400516119E-4	0.0	0.0	51.070852850643824	0.0
39	1.706742400516119E-4	0.0	0.0	51.8515168246399	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTC	25	3.8908518E-5	45.000004	5
ATCCGGC	65	0.0	45.000004	6
CATTGCG	20	7.033343E-4	45.0	1
TAGCGCG	20	7.033343E-4	45.0	1
TACGGGT	90	0.0	45.0	4
TCCGTCG	20	7.033343E-4	45.0	1
CGATAGC	20	7.033343E-4	45.0	10
TCGCGAG	40	6.8157533E-9	45.0	1
ATGCGAA	30	2.1655396E-6	44.999996	26
TACGGCT	3600	0.0	43.625	7
ACGGCTG	3665	0.0	42.851295	8
TCCGTTG	170	0.0	42.352943	1
CCCGCTT	75	0.0	42.0	40
CTACGGC	145	0.0	41.896553	6
ATACGGC	7060	0.0	41.87677	6
GATACGG	7120	0.0	41.65028	5
TGATACG	7155	0.0	41.603775	4
ACGGGTA	65	0.0	41.538464	5
CGTTTTT	1870	0.0	41.390373	1
ATGATAC	10210	0.0	41.36386	3
>>END_MODULE