SRR2933800.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933800.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773932
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	24405	3.153377816138886	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	19385	2.5047420186786433	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	16396	2.1185323775215394	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	12528	1.6187468666497833	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	7725	0.9981497082431015	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAGT	7134	0.921786410175571	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	6746	0.8716528067065323	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	5425	0.7009659763390065	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	5325	0.6880449445170893	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTA	4418	0.5708511858923005	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	4227	0.5461720151124388	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	3992	0.5158075903309334	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCGT	1681	0.21720254492642765	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1611	0.20815782265108562	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1606	0.20751177105998975	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG	1552	0.20053441387615448	No Hit
TGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT	1419	0.18334944155300464	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1398	0.18063602487040206	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	1162	0.1501423897706775	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT	1116	0.14419871513259563	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1009	0.13037321108314426	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGACAG	989	0.1277890047187608	No Hit
TGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	921	0.11900270307985714	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	906	0.11706454830656957	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	889	0.11486797289684365	No Hit
TTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	853	0.11021640144095346	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.2112469E-7	45.000004	27
GTAGCGG	30	2.1645792E-6	45.000004	2
TTCCACG	35	1.2112469E-7	45.000004	18
TACTCCG	35	1.2112469E-7	45.000004	1
AACACGT	30	2.1645792E-6	45.000004	41
GTCGAAT	20	7.0318574E-4	45.0	43
TTAGCCG	25	3.889621E-5	45.0	37
CGCATAG	25	3.889621E-5	45.0	27
CGACAAT	20	7.0318574E-4	45.0	20
TAGCCGA	25	3.889621E-5	45.0	38
CCGTACA	20	7.0318574E-4	45.0	30
ATCGGTT	20	7.0318574E-4	45.0	18
TAGGATA	25	3.889621E-5	45.0	17
TACGGCT	2820	0.0	43.08511	7
ATACGGC	5335	0.0	42.216496	6
TCGGCGT	80	0.0	42.1875	4
AATGATC	625	0.0	42.120003	2
ACGGCTG	2890	0.0	42.119377	8
GATACGG	5385	0.0	41.8663	5
TGATACG	5400	0.0	41.75	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content