##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933800.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773932
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.616303499532258	30.0	16.0	33.0	16.0	34.0
2	27.93516226231762	31.0	25.0	33.0	16.0	34.0
3	28.876517575187485	31.0	28.0	34.0	16.0	34.0
4	32.475742054857534	35.0	30.0	35.0	26.0	37.0
5	33.79982220660213	35.0	33.0	37.0	28.0	37.0
6	32.91643839510448	35.0	32.0	37.0	26.0	37.0
7	33.561625569171454	35.0	32.0	35.0	29.0	37.0
8	33.48320524283787	35.0	33.0	37.0	28.0	37.0
9	34.774219182047	37.0	32.0	39.0	29.0	39.0
10	33.57333719241484	35.0	31.0	39.0	27.0	39.0
11	34.10829116769949	35.0	32.0	39.0	27.0	39.0
12	34.217479055007416	37.0	32.0	39.0	26.0	39.0
13	34.50803558969005	37.0	33.0	39.0	27.0	39.0
14	34.37918706036189	37.0	32.0	39.0	25.0	40.0
15	34.785249866913375	37.0	32.0	39.0	27.0	40.0
16	34.25874237013071	36.0	32.0	39.0	27.0	40.0
17	34.269103745548705	36.0	32.0	38.0	27.0	40.0
18	34.272116930169574	36.0	32.0	39.0	27.0	40.0
19	33.875159574743	36.0	32.0	39.0	25.0	40.0
20	34.91916990123163	37.0	33.0	39.0	27.0	40.0
21	34.36279027097988	37.0	32.0	39.0	25.0	40.0
22	35.31377304466025	37.0	34.0	39.0	28.0	40.0
23	36.197633642232134	38.0	34.0	39.0	30.0	40.0
24	36.31772041988185	38.0	35.0	40.0	31.0	40.0
25	34.236321795713316	37.0	32.0	39.0	25.0	40.0
26	34.97685197149104	37.0	33.0	39.0	27.0	40.0
27	35.56619832233323	37.0	34.0	39.0	30.0	40.0
28	35.788801858561214	38.0	34.0	39.0	30.0	40.0
29	36.27070982980417	38.0	35.0	40.0	30.0	41.0
30	33.748096732012634	36.0	32.0	39.0	24.0	40.0
31	34.717838259692066	37.0	33.0	39.0	26.0	40.0
32	35.2247432590977	37.0	34.0	39.0	29.0	40.0
33	36.00852917310565	38.0	35.0	39.0	30.0	40.0
34	36.294307510220534	38.0	35.0	40.0	31.0	41.0
35	36.40423835685822	38.0	35.0	40.0	31.0	41.0
36	36.324828279487086	38.0	35.0	40.0	31.0	41.0
37	36.5321643245143	38.0	35.0	40.0	31.0	41.0
38	36.1598848477644	38.0	35.0	40.0	30.0	41.0
39	36.1466433743533	38.0	35.0	40.0	30.0	41.0
40	35.594854586707875	38.0	34.0	40.0	30.0	41.0
41	35.01070636696764	37.0	33.0	39.0	28.0	40.0
42	35.98729604151269	38.0	35.0	40.0	30.0	41.0
43	36.100680679956376	38.0	35.0	40.0	31.0	40.0
44	36.507954187189576	38.0	35.0	40.0	31.0	41.0
45	36.82789573244161	38.0	35.0	40.0	32.0	41.0
46	36.626757906379375	38.0	35.0	40.0	32.0	41.0
47	36.50785340314136	38.0	35.0	40.0	31.0	41.0
48	36.60547050645276	38.0	35.0	40.0	32.0	41.0
49	36.79601179431785	39.0	35.0	40.0	32.0	41.0
50	36.569467343384176	38.0	35.0	40.0	31.0	41.0
51	35.83710196761473	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	14.0
11	14.0
12	18.0
13	18.0
14	31.0
15	58.0
16	91.0
17	157.0
18	338.0
19	562.0
20	906.0
21	1364.0
22	1967.0
23	2562.0
24	3533.0
25	4868.0
26	6915.0
27	9237.0
28	12656.0
29	18071.0
30	25786.0
31	36529.0
32	51354.0
33	66709.0
34	84140.0
35	110502.0
36	132314.0
37	128449.0
38	68319.0
39	6438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.78961975987554	3.478083345823664	45.85803403916623	15.87426285513456
2	17.518593364791737	16.748887499160134	44.83132885059669	20.901190285451435
3	18.718828010729624	16.742039352294515	46.068517647545264	18.470614989430594
4	19.112402640025223	3.7507171172661162	54.4400541649654	22.696826077743264
5	26.166510752882683	4.307355168154308	44.54680772987808	24.979326349084932
6	19.84412067210039	15.13337089046583	46.372420315996756	18.650088121437026
7	69.14225022353385	1.559826961541841	23.473509300558707	5.824413514365603
8	66.41720461229151	10.896952187013847	12.548776895127736	10.137066305566897
9	62.36762402898446	3.5924344774476316	15.222526010037058	18.81741548353085
10	39.5360315893386	17.739930639901182	24.061803879410597	18.66223389134963
11	29.316658311066092	20.970059385062253	28.148597034364776	21.56468526950688
12	23.242352041264606	17.17980391042107	34.7839345058739	24.79390954244042
13	23.7989900921528	20.545861910348712	38.89501920065329	16.7601287968452
14	20.42479184217735	24.336246595308115	31.97050903696966	23.26845252554488
15	14.453466195996548	23.0717944212153	41.687771018642465	20.786968364145686
16	18.328612849707724	23.317552446468166	27.98734255722725	30.36649214659686
17	18.61197107756237	24.641699787578236	32.50518133376059	24.241147801098805
18	18.40303799300197	21.094618131825534	34.75085666440979	25.751487210762704
19	18.676188605717297	23.598843309231302	30.87441790751642	26.850550177534977
20	18.854369634541534	26.31652393233514	36.66950067964627	18.15960575347705
21	22.044701601691106	27.69093408723247	30.61754779489671	19.646816516179715
22	17.06622804070642	22.124424368032333	33.435366414620404	27.373981176640843
23	18.538062775541004	26.095057446907482	30.72711814474657	24.639761632804948
24	22.392664988655334	23.211858406164882	26.64394287870252	27.751533726477263
25	18.122910023102808	29.614358884243057	24.10651064951443	28.156220443139706
26	17.31108159373175	25.987296041512693	28.339311464056273	28.362310900699285
27	19.73816821116067	28.2606223802608	28.099367903123273	23.90184150545526
28	14.69483107042996	24.747781458836176	34.64322446933322	25.91416300140064
29	22.442281750851496	26.443537675144587	26.168965748928848	24.94521482507507
30	19.723825865838343	30.71419711292465	29.14752200451719	20.414455016719817
31	22.26862308316493	25.13799661985808	26.619909759513753	25.97347053746324
32	25.230381997384782	33.5479086017893	21.317376720435387	19.904332680390525
33	21.983714331491655	26.3944377542213	23.04827814329941	28.573569770987632
34	21.019030095667322	25.966493180279404	27.657856245768365	25.356620478284913
35	19.34511042313795	31.519952657339406	22.496808505139988	26.63812841438266
36	23.722109952812392	29.44871125628608	25.58687326535148	21.242305525550048
37	20.058480590025997	28.31191887659381	31.047301313293673	20.58229922008652
38	21.90205341037714	28.61414181090845	27.474636014533576	22.009168764180835
39	26.35231519048185	22.94064594822284	26.651437077159233	24.055601784136073
40	20.81113069365267	29.33888248579979	26.88995932459182	22.960027495955718
41	21.12162308833334	26.07929378808474	22.188512685869043	30.610570437712877
42	20.909201325181023	25.732880924939145	29.65803197180114	23.699885778078695
43	27.637441015489735	22.153496689631645	25.871006755115438	24.338055539763186
44	21.63484647229989	21.60060573797181	30.245680499061933	26.518867290666364
45	19.6494007225441	19.980695978458055	27.708765111146715	32.66113818785113
46	26.15604471710693	23.531524733439113	28.02520118046547	22.28722936898849
47	14.986975599923507	23.650269015882532	38.51371955158851	22.84903583260545
48	19.43568685620959	25.748903004398322	27.45241183979988	27.362998299592213
49	20.98168831370198	20.322715690784204	35.88700299251097	22.808593003002848
50	24.283399575156473	19.208147485825627	28.195629590196553	28.312823348821343
51	19.867766160334497	19.343301478682882	25.47278055436395	35.31615180661867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	139.0
1	368.5
2	598.0
3	1717.5
4	2837.0
5	1944.5
6	1052.0
7	1116.5
8	1181.0
9	1252.5
10	1324.0
11	1455.5
12	1587.0
13	1505.0
14	1423.0
15	1334.0
16	1245.0
17	1407.5
18	1570.0
19	1424.0
20	1278.0
21	1381.5
22	1485.0
23	2010.5
24	2536.0
25	2950.5
26	3739.5
27	4114.0
28	4653.0
29	5192.0
30	6162.5
31	7133.0
32	8623.5
33	10114.0
34	11391.0
35	12668.0
36	13547.5
37	14427.0
38	16809.0
39	19191.0
40	22963.5
41	26736.0
42	33523.0
43	40310.0
44	93146.5
45	145983.0
46	129506.0
47	113029.0
48	111114.5
49	109200.0
50	86337.0
51	63474.0
52	53138.5
53	42803.0
54	37691.5
55	32580.0
56	29870.5
57	27161.0
58	25056.5
59	22952.0
60	19213.0
61	15474.0
62	13839.0
63	12204.0
64	11010.5
65	9817.0
66	8135.5
67	6454.0
68	5301.0
69	4148.0
70	3435.5
71	2723.0
72	2293.0
73	1863.0
74	1566.0
75	953.0
76	637.0
77	502.5
78	368.0
79	279.5
80	191.0
81	122.0
82	53.0
83	44.0
84	35.0
85	20.0
86	5.0
87	3.0
88	1.0
89	1.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.229428873609155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.48759785293976	20.70340968019789
2	13.056057203191033	7.893543051872716
3	5.453235478005036	4.945445820401857
4	2.8573860732188816	3.4550859625924613
5	1.6891738178371518	2.5531379890735497
6	1.1865938272477625	2.1522032221589944
7	0.8283441157016082	1.7528258669932102
8	0.6648639480494873	1.607876594255116
9	0.56162079220315	1.5279728212660633
>10	4.9967806939752615	27.38590758260481
>50	0.11823489759053477	2.4997038454394604
>100	0.08241921684354318	4.799436597057336
>500	0.008630284486234888	1.731778334534645
>1k	0.005178170691740933	3.2860219964795303
>5k	0.002157571121558722	4.220524857929229
>10k+	0.0017260568972469776	9.485125777143129
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGC	24405	3.153377816138886	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCG	19385	2.5047420186786433	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTC	16396	2.1185323775215394	No Hit
GCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	12528	1.6187468666497833	No Hit
CCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	7725	0.9981497082431015	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGACAGT	7134	0.921786410175571	No Hit
CTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCT	6746	0.8716528067065323	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	5425	0.7009659763390065	No Hit
TCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGC	5325	0.6880449445170893	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTA	4418	0.5708511858923005	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTC	4227	0.5461720151124388	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCT	3992	0.5158075903309334	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGACAGTCGT	1681	0.21720254492642765	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1611	0.20815782265108562	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1606	0.20751177105998975	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATG	1552	0.20053441387615448	No Hit
TGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTGCTT	1419	0.18334944155300464	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	1398	0.18063602487040206	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCT	1162	0.1501423897706775	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTAT	1116	0.14419871513259563	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	1009	0.13037321108314426	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGACAG	989	0.1277890047187608	No Hit
TGCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	921	0.11900270307985714	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCC	906	0.11706454830656957	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	889	0.11486797289684365	No Hit
TTCTGTCTCTTATACACATCTGACGCAATGACAGTCGTATGCCGTCTTCTG	853	0.11021640144095346	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9944026090147455	0.0
2	0.0	0.0	0.0	4.784916504292367	0.0
3	0.0	0.0	0.0	5.872479752743135	0.0
4	0.0	0.0	0.0	8.014399197862344	0.0
5	0.0	0.0	0.0	12.428998930138565	0.0
6	0.0	0.0	0.0	13.607267821979192	0.0
7	0.0	0.0	0.0	15.274210137324726	0.0
8	0.0	0.0	0.0	17.435769550813248	0.0
9	0.0	0.0	0.0	18.121617919920613	0.0
10	0.0	0.0	0.0	22.166805352408222	0.0
11	0.0	0.0	0.0	24.917046975703293	0.0
12	0.0	0.0	0.0	30.477613020265345	0.0
13	0.0	0.0	0.0	31.51866055415721	0.0
14	0.0	0.0	0.0	31.950093806691026	0.0
15	0.0	0.0	0.0	33.02383155109234	0.0
16	0.0	0.0	0.0	34.16514629192229	0.0
17	0.0	0.0	0.0	35.59149899474372	0.0
18	0.0	0.0	0.0	37.026121157931186	0.0
19	0.0	0.0	0.0	39.01492637596068	0.0
20	0.0	0.0	0.0	40.18337528361665	0.0
21	0.0	0.0	0.0	41.4790963547185	0.0
22	1.292103182191717E-4	0.0	0.0	42.95997581182843	0.0
23	1.292103182191717E-4	0.0	0.0	44.22081009701111	0.0
24	1.292103182191717E-4	0.0	0.0	45.270773142860094	0.0
25	1.292103182191717E-4	0.0	0.0	46.11645467560457	0.0
26	1.292103182191717E-4	0.0	0.0	46.91006445010673	0.0
27	1.292103182191717E-4	0.0	0.0	47.79954828072751	0.0
28	1.292103182191717E-4	0.0	0.0	48.56395652331213	0.0
29	2.584206364383434E-4	0.0	0.0	49.48018688980427	0.0
30	2.584206364383434E-4	0.0	0.0	50.49151605050573	0.0
31	2.584206364383434E-4	0.0	0.0	51.30773763069624	0.0
32	2.584206364383434E-4	0.0	0.0	52.047079071546335	0.0
33	2.584206364383434E-4	0.0	0.0	52.80644811172041	0.0
34	2.584206364383434E-4	0.0	0.0	53.64528149759927	0.0
35	2.584206364383434E-4	0.0	0.0	54.546911098132654	0.0
36	2.584206364383434E-4	0.0	0.0	55.284314384209466	0.0
37	2.584206364383434E-4	0.0	0.0	56.02753213460614	0.0
38	3.876309546575151E-4	0.0	0.0	56.81274323842405	0.0
39	3.876309546575151E-4	0.0	0.0	58.106526154752615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	35	1.2112469E-7	45.000004	27
GTAGCGG	30	2.1645792E-6	45.000004	2
TTCCACG	35	1.2112469E-7	45.000004	18
TACTCCG	35	1.2112469E-7	45.000004	1
AACACGT	30	2.1645792E-6	45.000004	41
GTCGAAT	20	7.0318574E-4	45.0	43
TTAGCCG	25	3.889621E-5	45.0	37
CGCATAG	25	3.889621E-5	45.0	27
CGACAAT	20	7.0318574E-4	45.0	20
TAGCCGA	25	3.889621E-5	45.0	38
CCGTACA	20	7.0318574E-4	45.0	30
ATCGGTT	20	7.0318574E-4	45.0	18
TAGGATA	25	3.889621E-5	45.0	17
TACGGCT	2820	0.0	43.08511	7
ATACGGC	5335	0.0	42.216496	6
TCGGCGT	80	0.0	42.1875	4
AATGATC	625	0.0	42.120003	2
ACGGCTG	2890	0.0	42.119377	8
GATACGG	5385	0.0	41.8663	5
TGATACG	5400	0.0	41.75	4
>>END_MODULE