SRR2933799.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933799.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465626
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGC	14777	3.173577076881445	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCG	12317	2.6452560638795943	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	11025	2.367780149733907	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	8301	1.7827612719221004	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	5677	1.2192188580534593	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAGTTAT	4787	1.0280783289592934	No Hit
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	4659	1.0005884551120428	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3903	0.8382263877017176	TruSeq Adapter, Index 22 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	3679	0.7901191084690288	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTA	3156	0.6777972020462775	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTC	2782	0.5974752268988416	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCT	2585	0.5551665929308072	No Hit
TGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCTT	1490	0.3199993127531538	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAGTTATCGT	1054	0.22636192996095578	No Hit
CGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	1037	0.22271093109061782	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.2121874637584671	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATG	978	0.21003981736415064	No Hit
TTCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCT	832	0.17868418000713018	No Hit
TGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	827	0.17761035680997198	No Hit
TCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	789	0.16944930051156937	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTAT	743	0.1595701270977136	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTT	646	0.13873795707284386	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGGAGTTA	572	0.12284537375490201	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCC	551	0.11833531632683741	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTC	531	0.11404002353820447	No Hit
TTCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	500	0.10738231971582342	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCCTC	25	3.887218E-5	45.000004	9
GCACCTA	65	0.0	45.000004	9
GCCGATG	25	3.887218E-5	45.000004	9
TCGCTCG	25	3.887218E-5	45.000004	1
GATTGTA	25	3.887218E-5	45.000004	20
TACCGGG	25	3.887218E-5	45.000004	3
GGCTAAT	25	3.887218E-5	45.000004	8
CTATGCG	20	7.028958E-4	45.0	1
TCGTTAG	20	7.028958E-4	45.0	1
CCGGGTC	20	7.028958E-4	45.0	5
TGCCCCG	20	7.028958E-4	45.0	1
GGTAATC	40	6.7993824E-9	45.0	8
CCGGCAG	40	6.7993824E-9	45.0	11
TATGGCG	20	7.028958E-4	45.0	1
CGGTTCG	20	7.028958E-4	45.0	33
CGCATCC	20	7.028958E-4	45.0	45
CACGAGG	20	7.028958E-4	45.0	15
CGATTAT	20	7.028958E-4	45.0	10
CGTTAGG	20	7.028958E-4	45.0	2
CTTTGCG	85	0.0	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content